This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 171.64 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 133,898 (3.7%) Reads that were too short: 64,473 (1.8%) Reads written (passing filters): 69,425 (1.9%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 18,943,005 bp (1.7%) === Adapter 1 === Sequence: TGGAAC; Type: regular 5'; Length: 6; Trimmed: 133898 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2055 887.3 0 2055 7 617 887.3 0 617 8 25180 887.3 0 25180 9 28571 887.3 0 28571 10 55 887.3 0 55 11 35 887.3 0 35 12 59 887.3 0 59 13 71 887.3 0 71 14 47 887.3 0 47 15 38 887.3 0 38 16 56 887.3 0 56 17 55 887.3 0 55 18 39 887.3 0 39 19 68 887.3 0 68 20 50 887.3 0 50 21 45 887.3 0 45 22 48 887.3 0 48 23 55 887.3 0 55 24 56 887.3 0 56 25 44 887.3 0 44 26 37 887.3 0 37 27 51 887.3 0 51 28 57 887.3 0 57 29 49 887.3 0 49 30 49 887.3 0 49 31 55 887.3 0 55 32 40 887.3 0 40 33 46 887.3 0 46 34 55 887.3 0 55 35 48 887.3 0 48 36 78 887.3 0 78 37 44 887.3 0 44 38 42 887.3 0 42 39 35 887.3 0 35 40 69 887.3 0 69 41 47 887.3 0 47 42 47 887.3 0 47 43 50 887.3 0 50 44 68 887.3 0 68 45 47 887.3 0 47 46 69 887.3 0 69 47 44 887.3 0 44 48 38 887.3 0 38 49 27 887.3 0 27 50 39 887.3 0 39 51 46 887.3 0 46 52 60 887.3 0 60 53 72 887.3 0 72 54 57 887.3 0 57 55 55 887.3 0 55 56 49 887.3 0 49 57 40 887.3 0 40 58 63 887.3 0 63 59 54 887.3 0 54 60 52 887.3 0 52 61 65 887.3 0 65 62 67 887.3 0 67 63 99 887.3 0 99 64 55 887.3 0 55 65 48 887.3 0 48 66 105 887.3 0 105 67 91 887.3 0 91 68 66 887.3 0 66 69 53 887.3 0 53 70 46 887.3 0 46 71 51 887.3 0 51 72 61 887.3 0 61 73 71 887.3 0 71 74 55 887.3 0 55 75 72 887.3 0 72 76 49 887.3 0 49 77 59 887.3 0 59 78 57 887.3 0 57 79 43 887.3 0 43 80 50 887.3 0 50 81 56 887.3 0 56 82 47 887.3 0 47 83 38 887.3 0 38 84 57 887.3 0 57 85 53 887.3 0 53 86 54 887.3 0 54 87 52 887.3 0 52 88 43 887.3 0 43 89 50 887.3 0 50 90 54 887.3 0 54 91 52 887.3 0 52 92 76 887.3 0 76 93 84 887.3 0 84 94 69 887.3 0 69 95 70 887.3 0 70 96 55 887.3 0 55 97 49 887.3 0 49 98 68 887.3 0 68 99 66 887.3 0 66 100 128 887.3 0 128 101 74 887.3 0 74 102 76 887.3 0 76 103 68 887.3 0 68 104 55 887.3 0 55 105 71 887.3 0 71 106 61 887.3 0 61 107 57 887.3 0 57 108 51 887.3 0 51 109 79 887.3 0 79 110 65 887.3 0 65 111 54 887.3 0 54 112 55 887.3 0 55 113 56 887.3 0 56 114 61 887.3 0 61 115 62 887.3 0 62 116 56 887.3 0 56 117 45 887.3 0 45 118 64 887.3 0 64 119 56 887.3 0 56 120 45 887.3 0 45 121 67 887.3 0 67 122 60 887.3 0 60 123 60 887.3 0 60 124 118 887.3 0 118 125 53 887.3 0 53 126 52 887.3 0 52 127 58 887.3 0 58 128 51 887.3 0 51 129 44 887.3 0 44 130 63 887.3 0 63 131 73 887.3 0 73 132 72 887.3 0 72 133 125 887.3 0 125 134 68 887.3 0 68 135 54 887.3 0 54 136 65 887.3 0 65 137 209 887.3 0 209 138 535 887.3 0 535 139 481 887.3 0 481 140 355 887.3 0 355 141 56 887.3 0 56 142 62 887.3 0 62 143 59 887.3 0 59 144 85 887.3 0 85 145 65 887.3 0 65 146 63 887.3 0 63 147 45 887.3 0 45 148 62 887.3 0 62 149 80 887.3 0 80 150 61 887.3 0 61 151 70 887.3 0 70 152 64 887.3 0 64 153 47 887.3 0 47 154 67 887.3 0 67 155 51 887.3 0 51 156 73 887.3 0 73 157 62 887.3 0 62 158 57 887.3 0 57 159 57 887.3 0 57 160 75 887.3 0 75 161 51 887.3 0 51 162 70 887.3 0 70 163 58 887.3 0 58 164 49 887.3 0 49 165 67 887.3 0 67 166 55 887.3 0 55 167 44 887.3 0 44 168 68 887.3 0 68 169 56 887.3 0 56 170 77 887.3 0 77 171 68 887.3 0 68 172 76 887.3 0 76 173 71 887.3 0 71 174 62 887.3 0 62 175 45 887.3 0 45 176 50 887.3 0 50 177 47 887.3 0 47 178 75 887.3 0 75 179 72 887.3 0 72 180 94 887.3 0 94 181 71 887.3 0 71 182 63 887.3 0 63 183 73 887.3 0 73 184 66 887.3 0 66 185 83 887.3 0 83 186 91 887.3 0 91 187 100 887.3 0 100 188 79 887.3 0 79 189 76 887.3 0 76 190 58 887.3 0 58 191 56 887.3 0 56 192 66 887.3 0 66 193 67 887.3 0 67 194 66 887.3 0 66 195 58 887.3 0 58 196 76 887.3 0 76 197 78 887.3 0 78 198 84 887.3 0 84 199 77 887.3 0 77 200 65 887.3 0 65 201 75 887.3 0 75 202 71 887.3 0 71 203 74 887.3 0 74 204 58 887.3 0 58 205 64 887.3 0 64 206 66 887.3 0 66 207 63 887.3 0 63 208 54 887.3 0 54 209 66 887.3 0 66 210 84 887.3 0 84 211 77 887.3 0 77 212 78 887.3 0 78 213 61 887.3 0 61 214 101 887.3 0 101 215 88 887.3 0 88 216 78 887.3 0 78 217 86 887.3 0 86 218 67 887.3 0 67 219 67 887.3 0 67 220 70 887.3 0 70 221 65 887.3 0 65 222 57 887.3 0 57 223 71 887.3 0 71 224 77 887.3 0 77 225 103 887.3 0 103 226 88 887.3 0 88 227 72 887.3 0 72 228 77 887.3 0 77 229 82 887.3 0 82 230 71 887.3 0 71 231 72 887.3 0 72 232 69 887.3 0 69 233 75 887.3 0 75 234 85 887.3 0 85 235 73 887.3 0 73 236 66 887.3 0 66 237 52 887.3 0 52 238 180 887.3 0 180 239 244 887.3 0 244 240 135 887.3 0 135 241 67 887.3 0 67 242 93 887.3 0 93 243 73 887.3 0 73 244 65 887.3 0 65 245 72 887.3 0 72 246 64 887.3 0 64 247 93 887.3 0 93 248 101 887.3 0 101 249 64 887.3 0 64 250 66 887.3 0 66 251 88 887.3 0 88 252 78 887.3 0 78 253 57 887.3 0 57 254 71 887.3 0 71 255 88 887.3 0 88 256 69 887.3 0 69 257 106 887.3 0 106 258 116 887.3 0 116 259 137 887.3 0 137 260 174 887.3 0 174 261 96 887.3 0 96 262 87 887.3 0 87 263 136 887.3 0 136 264 94 887.3 0 94 265 108 887.3 0 108 266 132 887.3 0 132 267 118 887.3 0 118 268 135 887.3 0 135 269 130 887.3 0 130 270 182 887.3 0 182 271 195 887.3 0 195 272 433 887.3 0 433 273 6153 887.3 0 6153 274 6133 887.3 0 6133 275 3450 887.3 0 3450 276 210 887.3 0 210 277 260 887.3 0 260 278 185 887.3 0 185 279 173 887.3 0 173 280 171 887.3 0 171 281 93 887.3 0 93 282 123 887.3 0 123 283 255 887.3 0 255 284 337 887.3 0 337 285 391 887.3 0 391 286 910 887.3 0 910 287 911 887.3 0 911 288 1821 887.3 0 1821 289 176 887.3 0 176 290 136 887.3 0 136 291 222 887.3 0 222 292 396 887.3 0 396 293 3657 887.3 0 3657 294 2865 887.3 0 2865 295 1470 887.3 0 1470 296 213 887.3 0 213 297 723 887.3 0 723 298 2896 887.3 0 2896 299 8842 887.3 0 8842 300 8532 887.3 0 8532 301 5994 887.3 0 5994 Finished in 5.45 s (79 us/read; 0.76 M reads/minute). === Summary === Total reads processed: 69,425 Reads with adapters: 31,557 (45.5%) Reads that were too short: 4 (0.0%) Reads written (passing filters): 31,553 (45.4%) Total basepairs processed: 18,943,005 bp Total written (filtered): 8,546,692 bp (45.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 31557 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 2 18 13 0.0 3 0 0 13 19 338 0.0 3 0 329 7 1 1 20 29093 0.0 4 28583 489 17 0 4 21 81 0.0 4 13 67 1 22 1628 0.0 4 1601 18 8 0 1 23 25 0.0 4 1 8 0 16 24 2 0.0 4 0 0 0 0 2 30 2 0.0 4 2 35 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 4 0.0 4 0 0 0 0 4 127 355 0.0 4 0 0 0 0 355 128 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 137 2 0.0 4 0 0 0 0 2 139 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 1 212 1 0.0 4 1 229 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.88 s (60 us/read; 1.01 M reads/minute). === Summary === Total reads processed: 31,553 Reads with adapters: 3,104 (9.8%) Reads written (passing filters): 31,553 (100.0%) Total basepairs processed: 8,546,692 bp Total written (filtered): 8,514,263 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3104 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.9% C: 2.6% G: 94.8% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 8 493.0 0 8 4 5 123.3 0 5 7 2 1.9 0 2 8 1 0.5 0 1 9 903 0.1 0 19 884 10 1871 0.0 1 51 1820 11 301 0.0 1 2 299 12 1 0.0 1 0 1 13 2 0.0 1 0 2 169 1 0.0 1 1 223 8 0.0 1 8 224 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.