This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 175.85 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 198,175 (5.5%) Reads that were too short: 24,878 (0.7%) Reads written (passing filters): 173,297 (4.8%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 46,711,891 bp (4.3%) === Adapter 1 === Sequence: GCGAGA; Type: regular 5'; Length: 6; Trimmed: 198175 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 5118 887.3 0 5118 7 68267 887.3 0 68267 8 61125 887.3 0 61125 9 90 887.3 0 90 10 45 887.3 0 45 11 43 887.3 0 43 12 29 887.3 0 29 13 33 887.3 0 33 14 41 887.3 0 41 15 43 887.3 0 43 16 27 887.3 0 27 17 28 887.3 0 28 18 20 887.3 0 20 19 28 887.3 0 28 20 39 887.3 0 39 21 27 887.3 0 27 22 27 887.3 0 27 23 28 887.3 0 28 24 29 887.3 0 29 25 33 887.3 0 33 26 35 887.3 0 35 27 39 887.3 0 39 28 39 887.3 0 39 29 40 887.3 0 40 30 136 887.3 0 136 31 86 887.3 0 86 32 46 887.3 0 46 33 36 887.3 0 36 34 73 887.3 0 73 35 28 887.3 0 28 36 30 887.3 0 30 37 52 887.3 0 52 38 21 887.3 0 21 39 33 887.3 0 33 40 35 887.3 0 35 41 52 887.3 0 52 42 51 887.3 0 51 43 58 887.3 0 58 44 47 887.3 0 47 45 61 887.3 0 61 46 69 887.3 0 69 47 110 887.3 0 110 48 139 887.3 0 139 49 184 887.3 0 184 50 2963 887.3 0 2963 51 3042 887.3 0 3042 52 3590 887.3 0 3590 53 57 887.3 0 57 54 65 887.3 0 65 55 31 887.3 0 31 56 16 887.3 0 16 57 43 887.3 0 43 58 61 887.3 0 61 59 28 887.3 0 28 60 41 887.3 0 41 61 54 887.3 0 54 62 37 887.3 0 37 63 50 887.3 0 50 64 82 887.3 0 82 65 63 887.3 0 63 66 68 887.3 0 68 67 72 887.3 0 72 68 52 887.3 0 52 69 54 887.3 0 54 70 31 887.3 0 31 71 54 887.3 0 54 72 61 887.3 0 61 73 117 887.3 0 117 74 741 887.3 0 741 75 777 887.3 0 777 76 1565 887.3 0 1565 77 130 887.3 0 130 78 314 887.3 0 314 79 75 887.3 0 75 80 91 887.3 0 91 81 50 887.3 0 50 82 116 887.3 0 116 83 40 887.3 0 40 84 44 887.3 0 44 85 56 887.3 0 56 86 53 887.3 0 53 87 58 887.3 0 58 88 33 887.3 0 33 89 64 887.3 0 64 90 52 887.3 0 52 91 78 887.3 0 78 92 47 887.3 0 47 93 107 887.3 0 107 94 86 887.3 0 86 95 39 887.3 0 39 96 81 887.3 0 81 97 28 887.3 0 28 98 43 887.3 0 43 99 57 887.3 0 57 100 45 887.3 0 45 101 37 887.3 0 37 102 30 887.3 0 30 103 37 887.3 0 37 104 36 887.3 0 36 105 35 887.3 0 35 106 31 887.3 0 31 107 41 887.3 0 41 108 35 887.3 0 35 109 35 887.3 0 35 110 36 887.3 0 36 111 39 887.3 0 39 112 50 887.3 0 50 113 46 887.3 0 46 114 113 887.3 0 113 115 135 887.3 0 135 116 159 887.3 0 159 117 85 887.3 0 85 118 111 887.3 0 111 119 96 887.3 0 96 120 91 887.3 0 91 121 59 887.3 0 59 122 98 887.3 0 98 123 105 887.3 0 105 124 161 887.3 0 161 125 1150 887.3 0 1150 126 1197 887.3 0 1197 127 924 887.3 0 924 128 88 887.3 0 88 129 96 887.3 0 96 130 356 887.3 0 356 131 358 887.3 0 358 132 231 887.3 0 231 133 90 887.3 0 90 134 83 887.3 0 83 135 178 887.3 0 178 136 528 887.3 0 528 137 131 887.3 0 131 138 107 887.3 0 107 139 33 887.3 0 33 140 52 887.3 0 52 141 53 887.3 0 53 142 52 887.3 0 52 143 61 887.3 0 61 144 59 887.3 0 59 145 77 887.3 0 77 146 59 887.3 0 59 147 55 887.3 0 55 148 42 887.3 0 42 149 45 887.3 0 45 150 54 887.3 0 54 151 60 887.3 0 60 152 37 887.3 0 37 153 57 887.3 0 57 154 50 887.3 0 50 155 48 887.3 0 48 156 52 887.3 0 52 157 70 887.3 0 70 158 64 887.3 0 64 159 64 887.3 0 64 160 60 887.3 0 60 161 71 887.3 0 71 162 64 887.3 0 64 163 111 887.3 0 111 164 1485 887.3 0 1485 165 738 887.3 0 738 166 858 887.3 0 858 167 71 887.3 0 71 168 78 887.3 0 78 169 101 887.3 0 101 170 61 887.3 0 61 171 69 887.3 0 69 172 53 887.3 0 53 173 31 887.3 0 31 174 51 887.3 0 51 175 56 887.3 0 56 176 55 887.3 0 55 177 57 887.3 0 57 178 65 887.3 0 65 179 118 887.3 0 118 180 102 887.3 0 102 181 116 887.3 0 116 182 76 887.3 0 76 183 101 887.3 0 101 184 67 887.3 0 67 185 69 887.3 0 69 186 67 887.3 0 67 187 74 887.3 0 74 188 59 887.3 0 59 189 102 887.3 0 102 190 147 887.3 0 147 191 372 887.3 0 372 192 239 887.3 0 239 193 213 887.3 0 213 194 274 887.3 0 274 195 284 887.3 0 284 196 2170 887.3 0 2170 197 1970 887.3 0 1970 198 1379 887.3 0 1379 199 289 887.3 0 289 200 160 887.3 0 160 201 130 887.3 0 130 202 135 887.3 0 135 203 88 887.3 0 88 204 314 887.3 0 314 205 256 887.3 0 256 206 183 887.3 0 183 207 95 887.3 0 95 208 127 887.3 0 127 209 115 887.3 0 115 210 121 887.3 0 121 211 142 887.3 0 142 212 117 887.3 0 117 213 109 887.3 0 109 214 127 887.3 0 127 215 163 887.3 0 163 216 1031 887.3 0 1031 217 1544 887.3 0 1544 218 842 887.3 0 842 219 437 887.3 0 437 220 176 887.3 0 176 221 160 887.3 0 160 222 157 887.3 0 157 223 150 887.3 0 150 224 116 887.3 0 116 225 107 887.3 0 107 226 96 887.3 0 96 227 88 887.3 0 88 228 84 887.3 0 84 229 89 887.3 0 89 230 83 887.3 0 83 231 72 887.3 0 72 232 71 887.3 0 71 233 74 887.3 0 74 234 94 887.3 0 94 235 85 887.3 0 85 236 90 887.3 0 90 237 78 887.3 0 78 238 195 887.3 0 195 239 184 887.3 0 184 240 244 887.3 0 244 241 236 887.3 0 236 242 152 887.3 0 152 243 108 887.3 0 108 244 104 887.3 0 104 245 138 887.3 0 138 246 174 887.3 0 174 247 1065 887.3 0 1065 248 1537 887.3 0 1537 249 2109 887.3 0 2109 250 1126 887.3 0 1126 251 250 887.3 0 250 252 173 887.3 0 173 253 136 887.3 0 136 254 213 887.3 0 213 255 150 887.3 0 150 256 166 887.3 0 166 257 100 887.3 0 100 258 130 887.3 0 130 259 91 887.3 0 91 260 70 887.3 0 70 261 72 887.3 0 72 262 118 887.3 0 118 263 121 887.3 0 121 264 100 887.3 0 100 265 91 887.3 0 91 266 77 887.3 0 77 267 74 887.3 0 74 268 83 887.3 0 83 269 183 887.3 0 183 270 324 887.3 0 324 271 162 887.3 0 162 272 85 887.3 0 85 273 76 887.3 0 76 274 61 887.3 0 61 275 68 887.3 0 68 276 107 887.3 0 107 277 122 887.3 0 122 278 132 887.3 0 132 279 98 887.3 0 98 280 71 887.3 0 71 281 64 887.3 0 64 282 76 887.3 0 76 283 95 887.3 0 95 284 98 887.3 0 98 285 109 887.3 0 109 286 126 887.3 0 126 287 111 887.3 0 111 288 103 887.3 0 103 289 455 887.3 0 455 290 755 887.3 0 755 291 833 887.3 0 833 292 372 887.3 0 372 293 152 887.3 0 152 294 102 887.3 0 102 295 117 887.3 0 117 296 140 887.3 0 140 297 469 887.3 0 469 298 554 887.3 0 554 299 610 887.3 0 610 300 439 887.3 0 439 301 206 887.3 0 206 Finished in 12.88 s (74 us/read; 0.81 M reads/minute). === Summary === Total reads processed: 173,297 Reads with adapters: 71,917 (41.5%) Reads that were too short: 197 (0.1%) Reads written (passing filters): 71,720 (41.4%) Total basepairs processed: 46,711,891 bp Total written (filtered): 19,814,777 bp (42.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 71917 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 8 0.0 2 0 0 0 8 15 24 0.0 3 0 0 18 6 16 522 0.0 3 0 453 46 23 17 70352 0.0 3 66889 2878 470 115 18 151 0.0 3 8 119 9 15 19 132 0.0 3 5 0 70 57 20 172 0.0 3 0 0 0 172 21 1 0.0 3 1 22 1 0.0 3 0 0 0 1 24 1 0.0 3 1 25 1 0.0 3 0 1 26 8 0.0 3 0 0 0 8 27 47 0.0 3 1 0 0 46 28 1 0.0 3 0 0 0 1 29 2 0.0 3 0 0 0 2 30 6 0.0 3 0 0 0 6 31 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 40 2 0.0 3 0 0 0 2 42 2 0.0 3 0 0 0 2 43 1 0.0 3 0 0 1 45 1 0.0 3 0 0 0 1 46 67 0.0 3 0 0 2 65 47 3 0.0 3 0 0 0 3 48 1 0.0 3 0 0 0 1 49 11 0.0 3 0 0 1 10 50 2 0.0 3 0 0 0 2 53 6 0.0 3 0 0 1 5 55 2 0.0 3 0 0 0 2 60 3 0.0 3 0 0 0 3 61 6 0.0 3 0 0 0 6 62 20 0.0 3 0 0 0 20 63 1 0.0 3 0 0 0 1 65 6 0.0 3 0 0 0 6 67 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 74 2 0.0 3 0 0 0 2 75 3 0.0 3 0 0 0 3 76 1 0.0 3 0 0 0 1 77 2 0.0 3 0 0 0 2 78 4 0.0 3 0 0 0 4 79 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 84 3 0.0 3 0 0 0 3 85 1 0.0 3 0 0 0 1 87 5 0.0 3 0 0 0 5 88 2 0.0 3 0 0 0 2 89 2 0.0 3 0 0 0 2 90 9 0.0 3 0 0 0 9 91 2 0.0 3 0 0 0 2 92 1 0.0 3 0 0 0 1 95 7 0.0 3 0 0 0 7 96 4 0.0 3 0 0 0 4 97 38 0.0 3 0 0 2 36 98 3 0.0 3 0 0 0 3 99 5 0.0 3 0 0 0 5 100 10 0.0 3 0 0 0 10 101 1 0.0 3 0 0 1 102 2 0.0 3 0 0 0 2 105 7 0.0 3 0 0 1 6 107 1 0.0 3 0 0 1 108 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 1 1 112 2 0.0 3 0 0 1 1 113 3 0.0 3 0 0 0 3 114 2 0.0 3 0 0 0 2 115 2 0.0 3 0 0 0 2 116 3 0.0 3 0 0 0 3 117 17 0.0 3 0 0 1 16 118 18 0.0 3 0 0 1 17 119 2 0.0 3 0 0 0 2 120 6 0.0 3 0 0 0 6 121 19 0.0 3 0 0 0 19 122 1 0.0 3 0 0 0 1 123 4 0.0 3 0 0 0 4 124 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 129 2 0.0 3 0 0 0 2 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 134 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 137 3 0.0 3 0 0 0 3 139 1 0.0 3 0 0 0 1 140 2 0.0 3 0 0 0 2 141 4 0.0 3 0 0 0 4 143 1 0.0 3 0 0 0 1 144 2 0.0 3 0 0 0 2 146 2 0.0 3 0 0 0 2 148 3 0.0 3 0 0 0 3 157 2 0.0 3 0 0 0 2 158 8 0.0 3 0 0 0 8 159 3 0.0 3 0 0 0 3 160 2 0.0 3 0 0 0 2 161 2 0.0 3 0 0 0 2 164 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 166 3 0.0 3 0 0 0 3 167 2 0.0 3 0 0 0 2 168 1 0.0 3 0 0 0 1 169 3 0.0 3 0 0 1 2 172 1 0.0 3 0 0 1 173 1 0.0 3 0 0 0 1 176 4 0.0 3 0 0 1 3 177 4 0.0 3 0 0 0 4 178 2 0.0 3 0 0 0 2 181 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 187 2 0.0 3 0 0 0 2 189 1 0.0 3 0 0 1 192 2 0.0 3 0 0 1 1 194 2 0.0 3 0 0 0 2 195 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 205 4 0.0 3 0 0 0 4 208 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 217 4 0.0 3 0 0 0 4 218 6 0.0 3 0 0 0 6 219 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 243 2 0.0 3 0 0 0 2 244 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 249 3 0.0 3 0 0 0 3 250 3 0.0 3 0 0 0 3 251 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 270 4 0.0 3 0 0 0 4 275 1 0.0 3 0 0 0 1 279 4 0.0 3 0 0 0 4 280 6 0.0 3 0 0 0 6 281 10 0.0 3 0 0 0 10 282 1 0.0 3 0 0 0 1 293 1 0.0 3 0 0 0 1 294 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.29 s (60 us/read; 1.00 M reads/minute). === Summary === Total reads processed: 71,720 Reads with adapters: 686 (1.0%) Reads written (passing filters): 71,720 (100.0%) Total basepairs processed: 19,814,777 bp Total written (filtered): 19,812,697 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 686 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 15.3% C: 67.2% G: 10.5% T: 7.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 668 1120.6 0 668 4 14 280.2 0 14 5 4 70.0 0 4