This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 173.88 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 188,220 (5.2%) Reads that were too short: 16,611 (0.5%) Reads written (passing filters): 171,609 (4.7%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 47,054,252 bp (4.3%) === Adapter 1 === Sequence: GCGAGA; Type: regular 5'; Length: 6; Trimmed: 188220 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4717 887.3 0 4717 7 60749 887.3 0 60749 8 73740 887.3 0 73740 9 110 887.3 0 110 10 57 887.3 0 57 11 62 887.3 0 62 12 36 887.3 0 36 13 42 887.3 0 42 14 71 887.3 0 71 15 60 887.3 0 60 16 31 887.3 0 31 17 21 887.3 0 21 18 46 887.3 0 46 19 40 887.3 0 40 20 43 887.3 0 43 21 50 887.3 0 50 22 39 887.3 0 39 23 33 887.3 0 33 24 48 887.3 0 48 25 44 887.3 0 44 26 49 887.3 0 49 27 47 887.3 0 47 28 45 887.3 0 45 29 38 887.3 0 38 30 145 887.3 0 145 31 108 887.3 0 108 32 58 887.3 0 58 33 55 887.3 0 55 34 78 887.3 0 78 35 53 887.3 0 53 36 44 887.3 0 44 37 62 887.3 0 62 38 46 887.3 0 46 39 48 887.3 0 48 40 70 887.3 0 70 41 66 887.3 0 66 42 54 887.3 0 54 43 73 887.3 0 73 44 65 887.3 0 65 45 65 887.3 0 65 46 71 887.3 0 71 47 112 887.3 0 112 48 96 887.3 0 96 49 132 887.3 0 132 50 2774 887.3 0 2774 51 3082 887.3 0 3082 52 3510 887.3 0 3510 53 63 887.3 0 63 54 93 887.3 0 93 55 38 887.3 0 38 56 35 887.3 0 35 57 48 887.3 0 48 58 57 887.3 0 57 59 43 887.3 0 43 60 58 887.3 0 58 61 41 887.3 0 41 62 35 887.3 0 35 63 51 887.3 0 51 64 55 887.3 0 55 65 210 887.3 0 210 66 68 887.3 0 68 67 46 887.3 0 46 68 50 887.3 0 50 69 49 887.3 0 49 70 33 887.3 0 33 71 50 887.3 0 50 72 62 887.3 0 62 73 73 887.3 0 73 74 233 887.3 0 233 75 324 887.3 0 324 76 489 887.3 0 489 77 45 887.3 0 45 78 94 887.3 0 94 79 66 887.3 0 66 80 64 887.3 0 64 81 54 887.3 0 54 82 123 887.3 0 123 83 46 887.3 0 46 84 48 887.3 0 48 85 47 887.3 0 47 86 42 887.3 0 42 87 43 887.3 0 43 88 45 887.3 0 45 89 46 887.3 0 46 90 38 887.3 0 38 91 51 887.3 0 51 92 39 887.3 0 39 93 50 887.3 0 50 94 36 887.3 0 36 95 34 887.3 0 34 96 47 887.3 0 47 97 53 887.3 0 53 98 75 887.3 0 75 99 49 887.3 0 49 100 60 887.3 0 60 101 63 887.3 0 63 102 48 887.3 0 48 103 49 887.3 0 49 104 46 887.3 0 46 105 46 887.3 0 46 106 56 887.3 0 56 107 55 887.3 0 55 108 45 887.3 0 45 109 43 887.3 0 43 110 35 887.3 0 35 111 59 887.3 0 59 112 68 887.3 0 68 113 48 887.3 0 48 114 118 887.3 0 118 115 95 887.3 0 95 116 123 887.3 0 123 117 75 887.3 0 75 118 86 887.3 0 86 119 81 887.3 0 81 120 72 887.3 0 72 121 58 887.3 0 58 122 86 887.3 0 86 123 71 887.3 0 71 124 119 887.3 0 119 125 915 887.3 0 915 126 833 887.3 0 833 127 698 887.3 0 698 128 54 887.3 0 54 129 88 887.3 0 88 130 160 887.3 0 160 131 185 887.3 0 185 132 166 887.3 0 166 133 81 887.3 0 81 134 66 887.3 0 66 135 182 887.3 0 182 136 535 887.3 0 535 137 139 887.3 0 139 138 104 887.3 0 104 139 49 887.3 0 49 140 54 887.3 0 54 141 50 887.3 0 50 142 48 887.3 0 48 143 52 887.3 0 52 144 58 887.3 0 58 145 65 887.3 0 65 146 46 887.3 0 46 147 56 887.3 0 56 148 40 887.3 0 40 149 47 887.3 0 47 150 46 887.3 0 46 151 58 887.3 0 58 152 29 887.3 0 29 153 59 887.3 0 59 154 57 887.3 0 57 155 42 887.3 0 42 156 59 887.3 0 59 157 53 887.3 0 53 158 55 887.3 0 55 159 54 887.3 0 54 160 73 887.3 0 73 161 67 887.3 0 67 162 60 887.3 0 60 163 84 887.3 0 84 164 1407 887.3 0 1407 165 673 887.3 0 673 166 738 887.3 0 738 167 67 887.3 0 67 168 93 887.3 0 93 169 97 887.3 0 97 170 37 887.3 0 37 171 51 887.3 0 51 172 39 887.3 0 39 173 51 887.3 0 51 174 54 887.3 0 54 175 45 887.3 0 45 176 56 887.3 0 56 177 59 887.3 0 59 178 59 887.3 0 59 179 84 887.3 0 84 180 51 887.3 0 51 181 68 887.3 0 68 182 62 887.3 0 62 183 84 887.3 0 84 184 48 887.3 0 48 185 61 887.3 0 61 186 93 887.3 0 93 187 63 887.3 0 63 188 66 887.3 0 66 189 89 887.3 0 89 190 93 887.3 0 93 191 266 887.3 0 266 192 204 887.3 0 204 193 217 887.3 0 217 194 144 887.3 0 144 195 177 887.3 0 177 196 1438 887.3 0 1438 197 1390 887.3 0 1390 198 1075 887.3 0 1075 199 196 887.3 0 196 200 110 887.3 0 110 201 108 887.3 0 108 202 87 887.3 0 87 203 72 887.3 0 72 204 336 887.3 0 336 205 262 887.3 0 262 206 174 887.3 0 174 207 97 887.3 0 97 208 119 887.3 0 119 209 95 887.3 0 95 210 108 887.3 0 108 211 127 887.3 0 127 212 120 887.3 0 120 213 123 887.3 0 123 214 75 887.3 0 75 215 102 887.3 0 102 216 863 887.3 0 863 217 926 887.3 0 926 218 642 887.3 0 642 219 277 887.3 0 277 220 89 887.3 0 89 221 102 887.3 0 102 222 77 887.3 0 77 223 102 887.3 0 102 224 97 887.3 0 97 225 96 887.3 0 96 226 69 887.3 0 69 227 86 887.3 0 86 228 78 887.3 0 78 229 70 887.3 0 70 230 93 887.3 0 93 231 71 887.3 0 71 232 58 887.3 0 58 233 56 887.3 0 56 234 74 887.3 0 74 235 73 887.3 0 73 236 45 887.3 0 45 237 68 887.3 0 68 238 138 887.3 0 138 239 121 887.3 0 121 240 150 887.3 0 150 241 132 887.3 0 132 242 90 887.3 0 90 243 87 887.3 0 87 244 57 887.3 0 57 245 93 887.3 0 93 246 84 887.3 0 84 247 525 887.3 0 525 248 859 887.3 0 859 249 1625 887.3 0 1625 250 907 887.3 0 907 251 137 887.3 0 137 252 132 887.3 0 132 253 72 887.3 0 72 254 83 887.3 0 83 255 121 887.3 0 121 256 84 887.3 0 84 257 81 887.3 0 81 258 82 887.3 0 82 259 73 887.3 0 73 260 75 887.3 0 75 261 64 887.3 0 64 262 97 887.3 0 97 263 86 887.3 0 86 264 52 887.3 0 52 265 68 887.3 0 68 266 58 887.3 0 58 267 63 887.3 0 63 268 71 887.3 0 71 269 185 887.3 0 185 270 301 887.3 0 301 271 110 887.3 0 110 272 80 887.3 0 80 273 65 887.3 0 65 274 69 887.3 0 69 275 61 887.3 0 61 276 89 887.3 0 89 277 71 887.3 0 71 278 86 887.3 0 86 279 71 887.3 0 71 280 57 887.3 0 57 281 43 887.3 0 43 282 80 887.3 0 80 283 69 887.3 0 69 284 101 887.3 0 101 285 66 887.3 0 66 286 78 887.3 0 78 287 77 887.3 0 77 288 47 887.3 0 47 289 194 887.3 0 194 290 300 887.3 0 300 291 358 887.3 0 358 292 167 887.3 0 167 293 128 887.3 0 128 294 72 887.3 0 72 295 70 887.3 0 70 296 73 887.3 0 73 297 237 887.3 0 237 298 220 887.3 0 220 299 267 887.3 0 267 300 207 887.3 0 207 301 136 887.3 0 136 Finished in 13.52 s (79 us/read; 0.76 M reads/minute). === Summary === Total reads processed: 171,609 Reads with adapters: 76,324 (44.5%) Reads that were too short: 42 (0.0%) Reads written (passing filters): 76,282 (44.5%) Total basepairs processed: 47,054,252 bp Total written (filtered): 20,737,845 bp (44.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 76324 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 4 18 19 0.0 3 0 0 17 1 1 19 723 0.0 3 0 703 17 2 1 20 74456 0.0 4 73251 1142 40 10 13 21 185 0.0 4 32 149 4 22 25 0.0 4 9 0 14 2 23 40 0.0 4 2 1 0 34 3 24 5 0.0 4 1 0 0 0 4 28 1 0.0 4 1 50 1 0.0 4 0 0 0 1 56 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 84 16 0.0 4 0 0 0 0 16 86 2 0.0 4 0 0 0 0 2 92 114 0.0 4 0 0 0 0 114 123 1 0.0 4 0 0 0 0 1 126 6 0.0 4 0 0 0 0 6 127 682 0.0 4 0 0 0 0 682 136 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 153 1 0.0 4 1 162 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 185 9 0.0 4 0 0 0 0 9 186 7 0.0 4 0 0 0 0 7 187 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 228 6 0.0 4 0 0 0 0 6 229 3 0.0 4 0 0 0 0 3 234 1 0.0 4 0 0 0 0 1 235 2 0.0 4 0 0 0 0 2 236 1 0.0 4 0 0 0 0 1 244 3 0.0 4 0 0 0 0 3 282 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.59 s (60 us/read; 1.00 M reads/minute). === Summary === Total reads processed: 76,282 Reads with adapters: 6,342 (8.3%) Reads written (passing filters): 76,282 (100.0%) Total basepairs processed: 20,737,845 bp Total written (filtered): 20,661,933 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6342 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.1% C: 3.9% G: 93.7% T: 1.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 38 1191.9 0 38 4 18 298.0 0 18 5 4 74.5 0 4 6 1 18.6 0 1 8 1 1.2 0 1 9 112 0.3 0 2 110 10 570 0.1 1 16 554 11 5529 0.0 1 24 5505 12 10 0.0 1 0 10 50 1 0.0 1 1 51 1 0.0 1 1 122 1 0.0 1 1 123 42 0.0 1 35 7 124 2 0.0 1 2 125 1 0.0 1 1 151 2 0.0 1 0 2 170 1 0.0 1 0 1 209 1 0.0 1 1 224 6 0.0 1 6 258 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.