This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACTTCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 180.22 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 490,339 (13.5%) Reads that were too short: 124,859 (3.4%) Reads written (passing filters): 365,480 (10.1%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 99,289,103 bp (9.1%) === Adapter 1 === Sequence: ACTTCA; Type: regular 5'; Length: 6; Trimmed: 490339 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3871 887.3 0 3871 7 142148 887.3 0 142148 8 3961 887.3 0 3961 9 154675 887.3 0 154675 10 191 887.3 0 191 11 60 887.3 0 60 12 51 887.3 0 51 13 50 887.3 0 50 14 61 887.3 0 61 15 108 887.3 0 108 16 171 887.3 0 171 17 90 887.3 0 90 18 68 887.3 0 68 19 59 887.3 0 59 20 37 887.3 0 37 21 31 887.3 0 31 22 69 887.3 0 69 23 49 887.3 0 49 24 38 887.3 0 38 25 50 887.3 0 50 26 38 887.3 0 38 27 47 887.3 0 47 28 45 887.3 0 45 29 53 887.3 0 53 30 61 887.3 0 61 31 58 887.3 0 58 32 37 887.3 0 37 33 40 887.3 0 40 34 63 887.3 0 63 35 49 887.3 0 49 36 46 887.3 0 46 37 64 887.3 0 64 38 91 887.3 0 91 39 63 887.3 0 63 40 90 887.3 0 90 41 74 887.3 0 74 42 65 887.3 0 65 43 56 887.3 0 56 44 53 887.3 0 53 45 50 887.3 0 50 46 46 887.3 0 46 47 60 887.3 0 60 48 79 887.3 0 79 49 53 887.3 0 53 50 75 887.3 0 75 51 76 887.3 0 76 52 57 887.3 0 57 53 57 887.3 0 57 54 64 887.3 0 64 55 91 887.3 0 91 56 121 887.3 0 121 57 907 887.3 0 907 58 951 887.3 0 951 59 1698 887.3 0 1698 60 56 887.3 0 56 61 47 887.3 0 47 62 60 887.3 0 60 63 50 887.3 0 50 64 53 887.3 0 53 65 72 887.3 0 72 66 48 887.3 0 48 67 48 887.3 0 48 68 65 887.3 0 65 69 68 887.3 0 68 70 65 887.3 0 65 71 49 887.3 0 49 72 53 887.3 0 53 73 51 887.3 0 51 74 81 887.3 0 81 75 84 887.3 0 84 76 78 887.3 0 78 77 65 887.3 0 65 78 56 887.3 0 56 79 71 887.3 0 71 80 72 887.3 0 72 81 67 887.3 0 67 82 80 887.3 0 80 83 86 887.3 0 86 84 65 887.3 0 65 85 55 887.3 0 55 86 72 887.3 0 72 87 76 887.3 0 76 88 86 887.3 0 86 89 94 887.3 0 94 90 87 887.3 0 87 91 140 887.3 0 140 92 76 887.3 0 76 93 65 887.3 0 65 94 61 887.3 0 61 95 58 887.3 0 58 96 62 887.3 0 62 97 46 887.3 0 46 98 52 887.3 0 52 99 98 887.3 0 98 100 88 887.3 0 88 101 90 887.3 0 90 102 63 887.3 0 63 103 67 887.3 0 67 104 122 887.3 0 122 105 175 887.3 0 175 106 170 887.3 0 170 107 351 887.3 0 351 108 312 887.3 0 312 109 175 887.3 0 175 110 80 887.3 0 80 111 51 887.3 0 51 112 52 887.3 0 52 113 80 887.3 0 80 114 94 887.3 0 94 115 83 887.3 0 83 116 105 887.3 0 105 117 152 887.3 0 152 118 81 887.3 0 81 119 125 887.3 0 125 120 162 887.3 0 162 121 192 887.3 0 192 122 1037 887.3 0 1037 123 893 887.3 0 893 124 1168 887.3 0 1168 125 94 887.3 0 94 126 66 887.3 0 66 127 72 887.3 0 72 128 104 887.3 0 104 129 108 887.3 0 108 130 167 887.3 0 167 131 143 887.3 0 143 132 119 887.3 0 119 133 142 887.3 0 142 134 198 887.3 0 198 135 322 887.3 0 322 136 139 887.3 0 139 137 130 887.3 0 130 138 118 887.3 0 118 139 134 887.3 0 134 140 132 887.3 0 132 141 392 887.3 0 392 142 553 887.3 0 553 143 576 887.3 0 576 144 181 887.3 0 181 145 171 887.3 0 171 146 286 887.3 0 286 147 526 887.3 0 526 148 739 887.3 0 739 149 832 887.3 0 832 150 741 887.3 0 741 151 7433 887.3 0 7433 152 12393 887.3 0 12393 153 9269 887.3 0 9269 154 152 887.3 0 152 155 160 887.3 0 160 156 185 887.3 0 185 157 583 887.3 0 583 158 611 887.3 0 611 159 517 887.3 0 517 160 134 887.3 0 134 161 215 887.3 0 215 162 392 887.3 0 392 163 402 887.3 0 402 164 151 887.3 0 151 165 98 887.3 0 98 166 90 887.3 0 90 167 136 887.3 0 136 168 119 887.3 0 119 169 125 887.3 0 125 170 105 887.3 0 105 171 184 887.3 0 184 172 147 887.3 0 147 173 68 887.3 0 68 174 79 887.3 0 79 175 98 887.3 0 98 176 203 887.3 0 203 177 184 887.3 0 184 178 128 887.3 0 128 179 86 887.3 0 86 180 72 887.3 0 72 181 112 887.3 0 112 182 277 887.3 0 277 183 345 887.3 0 345 184 271 887.3 0 271 185 126 887.3 0 126 186 207 887.3 0 207 187 235 887.3 0 235 188 227 887.3 0 227 189 242 887.3 0 242 190 103 887.3 0 103 191 95 887.3 0 95 192 83 887.3 0 83 193 90 887.3 0 90 194 105 887.3 0 105 195 184 887.3 0 184 196 271 887.3 0 271 197 208 887.3 0 208 198 142 887.3 0 142 199 95 887.3 0 95 200 119 887.3 0 119 201 237 887.3 0 237 202 134 887.3 0 134 203 142 887.3 0 142 204 160 887.3 0 160 205 155 887.3 0 155 206 216 887.3 0 216 207 300 887.3 0 300 208 527 887.3 0 527 209 1130 887.3 0 1130 210 1597 887.3 0 1597 211 1565 887.3 0 1565 212 1638 887.3 0 1638 213 15314 887.3 0 15314 214 18150 887.3 0 18150 215 52893 887.3 0 52893 216 1011 887.3 0 1011 217 200 887.3 0 200 218 197 887.3 0 197 219 229 887.3 0 229 220 393 887.3 0 393 221 1742 887.3 0 1742 222 3796 887.3 0 3796 223 5148 887.3 0 5148 224 943 887.3 0 943 225 112 887.3 0 112 226 102 887.3 0 102 227 115 887.3 0 115 228 111 887.3 0 111 229 101 887.3 0 101 230 104 887.3 0 104 231 199 887.3 0 199 232 268 887.3 0 268 233 177 887.3 0 177 234 117 887.3 0 117 235 160 887.3 0 160 236 128 887.3 0 128 237 89 887.3 0 89 238 136 887.3 0 136 239 147 887.3 0 147 240 127 887.3 0 127 241 137 887.3 0 137 242 151 887.3 0 151 243 189 887.3 0 189 244 338 887.3 0 338 245 375 887.3 0 375 246 178 887.3 0 178 247 76 887.3 0 76 248 95 887.3 0 95 249 90 887.3 0 90 250 124 887.3 0 124 251 120 887.3 0 120 252 128 887.3 0 128 253 107 887.3 0 107 254 147 887.3 0 147 255 186 887.3 0 186 256 189 887.3 0 189 257 515 887.3 0 515 258 745 887.3 0 745 259 778 887.3 0 778 260 317 887.3 0 317 261 211 887.3 0 211 262 220 887.3 0 220 263 417 887.3 0 417 264 621 887.3 0 621 265 415 887.3 0 415 266 176 887.3 0 176 267 117 887.3 0 117 268 156 887.3 0 156 269 319 887.3 0 319 270 393 887.3 0 393 271 256 887.3 0 256 272 260 887.3 0 260 273 343 887.3 0 343 274 386 887.3 0 386 275 169 887.3 0 169 276 176 887.3 0 176 277 243 887.3 0 243 278 178 887.3 0 178 279 132 887.3 0 132 280 125 887.3 0 125 281 348 887.3 0 348 282 531 887.3 0 531 283 362 887.3 0 362 284 263 887.3 0 263 285 286 887.3 0 286 286 273 887.3 0 273 287 146 887.3 0 146 288 119 887.3 0 119 289 207 887.3 0 207 290 301 887.3 0 301 291 220 887.3 0 220 292 136 887.3 0 136 293 93 887.3 0 93 294 187 887.3 0 187 295 279 887.3 0 279 296 262 887.3 0 262 297 215 887.3 0 215 298 165 887.3 0 165 299 131 887.3 0 131 300 161 887.3 0 161 301 103 887.3 0 103 Finished in 26.65 s (73 us/read; 0.82 M reads/minute). === Summary === Total reads processed: 365,480 Reads with adapters: 160,306 (43.9%) Reads that were too short: 119 (0.0%) Reads written (passing filters): 160,187 (43.8%) Total basepairs processed: 99,289,103 bp Total written (filtered): 44,039,753 bp (44.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 160306 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 22 0.0 2 0 0 0 22 15 96 0.0 3 0 0 74 22 16 1552 0.0 3 0 1376 112 64 17 157194 0.0 3 148681 7057 1179 277 18 438 0.0 3 40 343 35 20 19 324 0.0 3 12 7 179 126 20 425 0.0 3 186 10 1 228 21 3 0.0 3 0 1 0 2 22 1 0.0 3 0 0 0 1 23 3 0.0 3 1 0 0 2 25 1 0.0 3 1 27 1 0.0 3 1 29 1 0.0 3 1 31 2 0.0 3 1 0 0 1 32 10 0.0 3 0 0 1 9 34 1 0.0 3 0 0 1 35 1 0.0 3 0 0 0 1 36 2 0.0 3 2 37 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 5 0.0 3 0 0 0 5 57 2 0.0 3 0 0 0 2 60 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 1 1 64 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 1 66 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 0 1 70 2 0.0 3 0 0 0 2 73 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 79 2 0.0 3 0 0 1 1 84 2 0.0 3 0 0 0 2 87 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 3 0.0 3 0 0 0 3 102 3 0.0 3 0 0 0 3 103 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 115 1 0.0 3 1 118 2 0.0 3 0 0 0 2 127 2 0.0 3 0 0 0 2 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 4 0.0 3 0 0 1 3 134 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 138 2 0.0 3 0 0 0 2 140 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 144 1 0.0 3 0 0 0 1 145 4 0.0 3 0 0 0 4 148 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 1 152 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 160 49 0.0 3 0 0 0 49 161 2 0.0 3 0 0 0 2 162 1 0.0 3 0 0 0 1 163 3 0.0 3 0 0 0 3 164 5 0.0 3 0 0 1 4 166 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 178 2 0.0 3 0 0 0 2 180 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 4 0.0 3 0 0 0 4 185 1 0.0 3 0 0 0 1 186 2 0.0 3 0 0 0 2 190 2 0.0 3 0 0 0 2 191 1 0.0 3 0 0 0 1 194 6 0.0 3 0 0 0 6 197 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 17 0.0 3 0 0 1 16 221 2 0.0 3 0 0 0 2 222 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 238 2 0.0 3 0 0 0 2 262 2 0.0 3 0 0 0 2 265 1 0.0 3 0 0 0 1 269 2 0.0 3 0 0 0 2 270 3 0.0 3 0 0 0 3 279 5 0.0 3 0 0 0 5 280 2 0.0 3 0 0 0 2 281 11 0.0 3 0 0 0 11 282 7 0.0 3 0 0 0 7 283 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 294 3 0.0 3 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 9.42 s (59 us/read; 1.02 M reads/minute). === Summary === Total reads processed: 160,187 Reads with adapters: 1,879 (1.2%) Reads written (passing filters): 160,187 (100.0%) Total basepairs processed: 44,039,753 bp Total written (filtered): 44,033,991 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1879 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 38.6% C: 10.4% G: 45.9% T: 5.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1760 2502.9 0 1760 4 114 625.7 0 114 5 4 156.4 0 4 6 1 39.1 0 1