This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCAGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 184.66 s (51 us/read; 1.18 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 699,170 (19.2%) Reads that were too short: 21,439 (0.6%) Reads written (passing filters): 677,731 (18.6%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 148,426,813 bp (13.6%) === Adapter 1 === Sequence: TCCAGC; Type: regular 5'; Length: 6; Trimmed: 699170 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 762 887.3 0 762 7 1752 887.3 0 1752 8 31883 887.3 0 31883 9 21283 887.3 0 21283 10 128 887.3 0 128 11 114 887.3 0 114 12 166 887.3 0 166 13 135 887.3 0 135 14 110 887.3 0 110 15 108 887.3 0 108 16 86 887.3 0 86 17 123 887.3 0 123 18 199 887.3 0 199 19 217 887.3 0 217 20 185 887.3 0 185 21 149 887.3 0 149 22 188 887.3 0 188 23 196 887.3 0 196 24 214 887.3 0 214 25 126 887.3 0 126 26 115 887.3 0 115 27 105 887.3 0 105 28 130 887.3 0 130 29 166 887.3 0 166 30 183 887.3 0 183 31 153 887.3 0 153 32 126 887.3 0 126 33 140 887.3 0 140 34 151 887.3 0 151 35 174 887.3 0 174 36 307 887.3 0 307 37 228 887.3 0 228 38 580 887.3 0 580 39 205 887.3 0 205 40 185 887.3 0 185 41 146 887.3 0 146 42 209 887.3 0 209 43 151 887.3 0 151 44 216 887.3 0 216 45 263 887.3 0 263 46 309 887.3 0 309 47 371 887.3 0 371 48 731 887.3 0 731 49 2022 887.3 0 2022 50 492 887.3 0 492 51 647 887.3 0 647 52 1270 887.3 0 1270 53 753 887.3 0 753 54 851 887.3 0 851 55 1328 887.3 0 1328 56 1397 887.3 0 1397 57 1653 887.3 0 1653 58 1760 887.3 0 1760 59 3402 887.3 0 3402 60 4775 887.3 0 4775 61 9979 887.3 0 9979 62 157226 887.3 0 157226 63 143165 887.3 0 143165 64 92895 887.3 0 92895 65 1337 887.3 0 1337 66 126 887.3 0 126 67 103 887.3 0 103 68 114 887.3 0 114 69 139 887.3 0 139 70 198 887.3 0 198 71 746 887.3 0 746 72 1058 887.3 0 1058 73 857 887.3 0 857 74 298 887.3 0 298 75 383 887.3 0 383 76 3323 887.3 0 3323 77 6145 887.3 0 6145 78 5473 887.3 0 5473 79 439 887.3 0 439 80 103 887.3 0 103 81 92 887.3 0 92 82 119 887.3 0 119 83 128 887.3 0 128 84 100 887.3 0 100 85 103 887.3 0 103 86 102 887.3 0 102 87 82 887.3 0 82 88 91 887.3 0 91 89 99 887.3 0 99 90 103 887.3 0 103 91 114 887.3 0 114 92 101 887.3 0 101 93 142 887.3 0 142 94 116 887.3 0 116 95 111 887.3 0 111 96 72 887.3 0 72 97 69 887.3 0 69 98 120 887.3 0 120 99 420 887.3 0 420 100 92 887.3 0 92 101 89 887.3 0 89 102 129 887.3 0 129 103 166 887.3 0 166 104 215 887.3 0 215 105 188 887.3 0 188 106 176 887.3 0 176 107 802 887.3 0 802 108 1273 887.3 0 1273 109 1373 887.3 0 1373 110 285 887.3 0 285 111 235 887.3 0 235 112 172 887.3 0 172 113 90 887.3 0 90 114 92 887.3 0 92 115 125 887.3 0 125 116 99 887.3 0 99 117 104 887.3 0 104 118 110 887.3 0 110 119 102 887.3 0 102 120 110 887.3 0 110 121 110 887.3 0 110 122 97 887.3 0 97 123 99 887.3 0 99 124 107 887.3 0 107 125 145 887.3 0 145 126 379 887.3 0 379 127 420 887.3 0 420 128 260 887.3 0 260 129 233 887.3 0 233 130 127 887.3 0 127 131 111 887.3 0 111 132 156 887.3 0 156 133 137 887.3 0 137 134 153 887.3 0 153 135 128 887.3 0 128 136 137 887.3 0 137 137 316 887.3 0 316 138 156 887.3 0 156 139 226 887.3 0 226 140 323 887.3 0 323 141 319 887.3 0 319 142 574 887.3 0 574 143 819 887.3 0 819 144 9922 887.3 0 9922 145 12984 887.3 0 12984 146 10021 887.3 0 10021 147 459 887.3 0 459 148 302 887.3 0 302 149 199 887.3 0 199 150 172 887.3 0 172 151 156 887.3 0 156 152 189 887.3 0 189 153 182 887.3 0 182 154 224 887.3 0 224 155 283 887.3 0 283 156 1878 887.3 0 1878 157 2215 887.3 0 2215 158 2036 887.3 0 2036 159 462 887.3 0 462 160 431 887.3 0 431 161 569 887.3 0 569 162 1255 887.3 0 1255 163 2492 887.3 0 2492 164 47285 887.3 0 47285 165 24987 887.3 0 24987 166 21238 887.3 0 21238 167 363 887.3 0 363 168 392 887.3 0 392 169 1150 887.3 0 1150 170 1581 887.3 0 1581 171 1726 887.3 0 1726 172 663 887.3 0 663 173 537 887.3 0 537 174 430 887.3 0 430 175 104 887.3 0 104 176 89 887.3 0 89 177 100 887.3 0 100 178 112 887.3 0 112 179 259 887.3 0 259 180 331 887.3 0 331 181 313 887.3 0 313 182 128 887.3 0 128 183 196 887.3 0 196 184 324 887.3 0 324 185 413 887.3 0 413 186 319 887.3 0 319 187 166 887.3 0 166 188 226 887.3 0 226 189 937 887.3 0 937 190 1688 887.3 0 1688 191 1663 887.3 0 1663 192 762 887.3 0 762 193 104 887.3 0 104 194 85 887.3 0 85 195 86 887.3 0 86 196 111 887.3 0 111 197 127 887.3 0 127 198 210 887.3 0 210 199 160 887.3 0 160 200 103 887.3 0 103 201 64 887.3 0 64 202 100 887.3 0 100 203 131 887.3 0 131 204 147 887.3 0 147 205 116 887.3 0 116 206 166 887.3 0 166 207 145 887.3 0 145 208 125 887.3 0 125 209 106 887.3 0 106 210 81 887.3 0 81 211 82 887.3 0 82 212 152 887.3 0 152 213 161 887.3 0 161 214 169 887.3 0 169 215 187 887.3 0 187 216 305 887.3 0 305 217 408 887.3 0 408 218 295 887.3 0 295 219 156 887.3 0 156 220 96 887.3 0 96 221 106 887.3 0 106 222 103 887.3 0 103 223 124 887.3 0 124 224 138 887.3 0 138 225 151 887.3 0 151 226 516 887.3 0 516 227 286 887.3 0 286 228 225 887.3 0 225 229 659 887.3 0 659 230 470 887.3 0 470 231 1463 887.3 0 1463 232 134 887.3 0 134 233 92 887.3 0 92 234 104 887.3 0 104 235 92 887.3 0 92 236 170 887.3 0 170 237 165 887.3 0 165 238 239 887.3 0 239 239 122 887.3 0 122 240 156 887.3 0 156 241 136 887.3 0 136 242 83 887.3 0 83 243 137 887.3 0 137 244 179 887.3 0 179 245 188 887.3 0 188 246 160 887.3 0 160 247 204 887.3 0 204 248 177 887.3 0 177 249 142 887.3 0 142 250 115 887.3 0 115 251 100 887.3 0 100 252 137 887.3 0 137 253 102 887.3 0 102 254 110 887.3 0 110 255 89 887.3 0 89 256 91 887.3 0 91 257 98 887.3 0 98 258 107 887.3 0 107 259 190 887.3 0 190 260 280 887.3 0 280 261 413 887.3 0 413 262 343 887.3 0 343 263 175 887.3 0 175 264 148 887.3 0 148 265 200 887.3 0 200 266 206 887.3 0 206 267 221 887.3 0 221 268 174 887.3 0 174 269 90 887.3 0 90 270 103 887.3 0 103 271 185 887.3 0 185 272 196 887.3 0 196 273 167 887.3 0 167 274 126 887.3 0 126 275 148 887.3 0 148 276 160 887.3 0 160 277 301 887.3 0 301 278 161 887.3 0 161 279 255 887.3 0 255 280 169 887.3 0 169 281 289 887.3 0 289 282 142 887.3 0 142 283 117 887.3 0 117 284 164 887.3 0 164 285 227 887.3 0 227 286 300 887.3 0 300 287 831 887.3 0 831 288 894 887.3 0 894 289 778 887.3 0 778 290 252 887.3 0 252 291 170 887.3 0 170 292 173 887.3 0 173 293 208 887.3 0 208 294 167 887.3 0 167 295 160 887.3 0 160 296 261 887.3 0 261 297 222 887.3 0 222 298 227 887.3 0 227 299 166 887.3 0 166 300 127 887.3 0 127 301 155 887.3 0 155 Finished in 40.45 s (60 us/read; 1.01 M reads/minute). === Summary === Total reads processed: 677,731 Reads with adapters: 34,325 (5.1%) Reads that were too short: 894 (0.1%) Reads written (passing filters): 33,431 (4.9%) Total basepairs processed: 148,426,813 bp Total written (filtered): 8,989,082 bp (6.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 34325 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 12 0.0 3 0 0 6 6 16 292 0.0 3 0 247 31 14 17 31383 0.0 3 29937 1210 201 35 18 92 0.0 3 4 71 9 8 19 84 0.0 3 9 2 42 31 20 64 0.0 3 0 0 0 64 21 18 0.0 3 0 0 0 18 25 1 0.0 3 0 0 0 1 26 4 0.0 3 0 0 0 4 27 3 0.0 3 0 0 0 3 28 31 0.0 3 0 0 0 31 29 6 0.0 3 0 0 0 6 30 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 36 2 0.0 3 0 0 0 2 37 3 0.0 3 1 0 0 2 38 32 0.0 3 1 1 0 30 42 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 1 44 2 0.0 3 0 0 0 2 45 1 0.0 3 0 0 1 46 1 0.0 3 0 0 0 1 47 3 0.0 3 1 0 0 2 51 1 0.0 3 0 0 0 1 53 12 0.0 3 0 0 0 12 54 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 0 2 58 1 0.0 3 0 0 0 1 60 12 0.0 3 0 0 0 12 61 10 0.0 3 0 0 1 9 62 6 0.0 3 0 0 0 6 63 1 0.0 3 0 0 0 1 64 6 0.0 3 0 0 0 6 65 2 0.0 3 0 0 0 2 66 1 0.0 3 0 0 0 1 68 3 0.0 3 0 0 0 3 72 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 5 0.0 3 0 0 0 5 82 1 0.0 3 0 0 0 1 84 6 0.0 3 0 0 1 5 85 4 0.0 3 0 0 0 4 86 4 0.0 3 0 0 0 4 87 3 0.0 3 0 0 0 3 89 4 0.0 3 0 0 0 4 90 3 0.0 3 0 0 0 3 91 1 0.0 3 0 0 0 1 92 3 0.0 3 0 0 0 3 94 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 99 3 0.0 3 0 0 0 3 100 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 103 5 0.0 3 0 0 0 5 104 31 0.0 3 0 0 0 31 105 5 0.0 3 0 0 0 5 106 1 0.0 3 0 0 0 1 107 13 0.0 3 0 0 0 13 108 15 0.0 3 0 0 0 15 109 81 0.0 3 0 0 1 80 110 7 0.0 3 0 0 0 7 111 6 0.0 3 0 0 0 6 112 12 0.0 3 0 0 1 11 113 1 0.0 3 0 0 0 1 114 8 0.0 3 0 0 0 8 115 1 0.0 3 0 0 0 1 116 2 0.0 3 0 0 0 2 117 7 0.0 3 0 0 0 7 118 6 0.0 3 0 0 0 6 119 3 0.0 3 0 0 1 2 120 1 0.0 3 0 0 0 1 121 2 0.0 3 0 0 0 2 122 6 0.0 3 0 0 0 6 123 13 0.0 3 0 0 1 12 124 36 0.0 3 0 0 2 34 125 4 0.0 3 0 0 0 4 126 5 0.0 3 0 0 0 5 127 15 0.0 3 0 0 1 14 128 33 0.0 3 0 0 0 33 129 782 0.0 3 0 0 11 771 130 129 0.0 3 0 0 1 128 131 6 0.0 3 0 0 0 6 132 112 0.0 3 0 0 2 110 133 17 0.0 3 0 0 0 17 134 1 0.0 3 0 0 0 1 135 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 2 0.0 3 0 0 0 2 144 5 0.0 3 0 0 0 5 145 4 0.0 3 0 0 0 4 146 3 0.0 3 0 0 0 3 147 5 0.0 3 0 0 0 5 148 11 0.0 3 0 0 0 11 149 4 0.0 3 0 0 0 4 150 8 0.0 3 0 0 0 8 151 6 0.0 3 0 0 0 6 152 4 0.0 3 0 0 0 4 153 7 0.0 3 0 0 0 7 154 3 0.0 3 0 0 0 3 155 44 0.0 3 0 0 1 43 156 8 0.0 3 0 0 0 8 157 1 0.0 3 0 0 0 1 159 2 0.0 3 0 0 0 2 160 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 164 21 0.0 3 0 0 0 21 165 2 0.0 3 0 0 0 2 166 1 0.0 3 0 0 0 1 167 4 0.0 3 0 0 1 3 168 3 0.0 3 0 0 0 3 169 2 0.0 3 0 0 0 2 170 2 0.0 3 0 0 0 2 172 2 0.0 3 0 0 0 2 173 2 0.0 3 0 0 0 2 174 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 176 3 0.0 3 0 0 0 3 177 5 0.0 3 0 0 0 5 179 4 0.0 3 0 0 0 4 180 88 0.0 3 0 0 5 83 181 11 0.0 3 0 0 0 11 182 2 0.0 3 0 0 0 2 183 5 0.0 3 0 0 0 5 184 4 0.0 3 0 0 1 3 185 1 0.0 3 0 0 0 1 189 41 0.0 3 0 0 0 41 190 15 0.0 3 0 0 1 14 191 1 0.0 3 0 0 0 1 192 9 0.0 3 0 0 0 9 194 2 0.0 3 0 0 0 2 195 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 204 2 0.0 3 0 0 0 2 205 4 0.0 3 0 0 0 4 206 5 0.0 3 0 0 0 5 207 1 0.0 3 0 0 0 1 208 3 0.0 3 0 0 0 3 209 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 68 0.0 3 0 0 3 65 220 9 0.0 3 0 0 0 9 221 7 0.0 3 0 0 0 7 222 2 0.0 3 0 0 0 2 223 1 0.0 3 0 0 1 224 3 0.0 3 0 0 1 2 225 1 0.0 3 0 0 0 1 226 2 0.0 3 0 0 0 2 227 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 229 14 0.0 3 0 1 0 13 230 53 0.0 3 0 0 4 49 231 224 0.0 3 0 0 16 208 232 10 0.0 3 0 0 0 10 233 1 0.0 3 0 0 0 1 234 3 0.0 3 0 0 0 3 235 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 237 2 0.0 3 0 0 0 2 238 1 0.0 3 0 0 0 1 239 7 0.0 3 0 0 1 6 240 2 0.0 3 0 0 0 2 241 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 279 3 0.0 3 0 0 0 3 280 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 1 2 282 3 0.0 3 0 0 0 3 290 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.90 s (57 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 33,431 Reads with adapters: 204 (0.6%) Reads written (passing filters): 33,431 (100.0%) Total basepairs processed: 8,989,082 bp Total written (filtered): 8,988,447 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 204 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 60.8% C: 23.5% G: 8.8% T: 6.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 187 522.4 0 187 4 11 130.6 0 11 5 6 32.6 0 6