This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTTGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 176.25 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 269,685 (7.4%) Reads that were too short: 16,031 (0.4%) Reads written (passing filters): 253,654 (7.0%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 57,772,802 bp (5.3%) === Adapter 1 === Sequence: CTTGGT; Type: regular 5'; Length: 6; Trimmed: 269685 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3143 887.3 0 3143 7 79503 887.3 0 79503 8 57043 887.3 0 57043 9 159 887.3 0 159 10 172 887.3 0 172 11 71 887.3 0 71 12 109 887.3 0 109 13 81 887.3 0 81 14 87 887.3 0 87 15 88 887.3 0 88 16 64 887.3 0 64 17 115 887.3 0 115 18 110 887.3 0 110 19 172 887.3 0 172 20 247 887.3 0 247 21 200 887.3 0 200 22 89 887.3 0 89 23 115 887.3 0 115 24 63 887.3 0 63 25 70 887.3 0 70 26 120 887.3 0 120 27 77 887.3 0 77 28 72 887.3 0 72 29 99 887.3 0 99 30 103 887.3 0 103 31 100 887.3 0 100 32 86 887.3 0 86 33 98 887.3 0 98 34 121 887.3 0 121 35 101 887.3 0 101 36 93 887.3 0 93 37 97 887.3 0 97 38 167 887.3 0 167 39 188 887.3 0 188 40 140 887.3 0 140 41 223 887.3 0 223 42 89 887.3 0 89 43 89 887.3 0 89 44 101 887.3 0 101 45 109 887.3 0 109 46 113 887.3 0 113 47 106 887.3 0 106 48 100 887.3 0 100 49 98 887.3 0 98 50 98 887.3 0 98 51 111 887.3 0 111 52 91 887.3 0 91 53 79 887.3 0 79 54 89 887.3 0 89 55 72 887.3 0 72 56 83 887.3 0 83 57 115 887.3 0 115 58 104 887.3 0 104 59 83 887.3 0 83 60 87 887.3 0 87 61 87 887.3 0 87 62 87 887.3 0 87 63 65 887.3 0 65 64 75 887.3 0 75 65 98 887.3 0 98 66 75 887.3 0 75 67 81 887.3 0 81 68 107 887.3 0 107 69 90 887.3 0 90 70 82 887.3 0 82 71 95 887.3 0 95 72 95 887.3 0 95 73 139 887.3 0 139 74 95 887.3 0 95 75 121 887.3 0 121 76 219 887.3 0 219 77 278 887.3 0 278 78 166 887.3 0 166 79 96 887.3 0 96 80 111 887.3 0 111 81 87 887.3 0 87 82 104 887.3 0 104 83 123 887.3 0 123 84 93 887.3 0 93 85 91 887.3 0 91 86 144 887.3 0 144 87 124 887.3 0 124 88 107 887.3 0 107 89 96 887.3 0 96 90 134 887.3 0 134 91 156 887.3 0 156 92 195 887.3 0 195 93 723 887.3 0 723 94 781 887.3 0 781 95 589 887.3 0 589 96 744 887.3 0 744 97 906 887.3 0 906 98 434 887.3 0 434 99 87 887.3 0 87 100 95 887.3 0 95 101 70 887.3 0 70 102 94 887.3 0 94 103 72 887.3 0 72 104 89 887.3 0 89 105 158 887.3 0 158 106 70 887.3 0 70 107 135 887.3 0 135 108 120 887.3 0 120 109 101 887.3 0 101 110 118 887.3 0 118 111 103 887.3 0 103 112 96 887.3 0 96 113 90 887.3 0 90 114 68 887.3 0 68 115 94 887.3 0 94 116 103 887.3 0 103 117 89 887.3 0 89 118 76 887.3 0 76 119 67 887.3 0 67 120 58 887.3 0 58 121 71 887.3 0 71 122 85 887.3 0 85 123 84 887.3 0 84 124 60 887.3 0 60 125 86 887.3 0 86 126 79 887.3 0 79 127 93 887.3 0 93 128 94 887.3 0 94 129 193 887.3 0 193 130 668 887.3 0 668 131 524 887.3 0 524 132 437 887.3 0 437 133 138 887.3 0 138 134 156 887.3 0 156 135 149 887.3 0 149 136 119 887.3 0 119 137 180 887.3 0 180 138 100 887.3 0 100 139 92 887.3 0 92 140 157 887.3 0 157 141 101 887.3 0 101 142 353 887.3 0 353 143 960 887.3 0 960 144 2215 887.3 0 2215 145 242 887.3 0 242 146 65 887.3 0 65 147 97 887.3 0 97 148 56 887.3 0 56 149 88 887.3 0 88 150 91 887.3 0 91 151 136 887.3 0 136 152 339 887.3 0 339 153 82 887.3 0 82 154 127 887.3 0 127 155 105 887.3 0 105 156 203 887.3 0 203 157 131 887.3 0 131 158 134 887.3 0 134 159 108 887.3 0 108 160 106 887.3 0 106 161 127 887.3 0 127 162 201 887.3 0 201 163 244 887.3 0 244 164 339 887.3 0 339 165 364 887.3 0 364 166 461 887.3 0 461 167 1093 887.3 0 1093 168 2378 887.3 0 2378 169 44542 887.3 0 44542 170 17602 887.3 0 17602 171 18455 887.3 0 18455 172 309 887.3 0 309 173 93 887.3 0 93 174 231 887.3 0 231 175 113 887.3 0 113 176 297 887.3 0 297 177 67 887.3 0 67 178 106 887.3 0 106 179 83 887.3 0 83 180 104 887.3 0 104 181 83 887.3 0 83 182 66 887.3 0 66 183 70 887.3 0 70 184 105 887.3 0 105 185 93 887.3 0 93 186 77 887.3 0 77 187 72 887.3 0 72 188 65 887.3 0 65 189 67 887.3 0 67 190 81 887.3 0 81 191 71 887.3 0 71 192 131 887.3 0 131 193 172 887.3 0 172 194 90 887.3 0 90 195 56 887.3 0 56 196 87 887.3 0 87 197 65 887.3 0 65 198 114 887.3 0 114 199 58 887.3 0 58 200 85 887.3 0 85 201 84 887.3 0 84 202 106 887.3 0 106 203 100 887.3 0 100 204 129 887.3 0 129 205 90 887.3 0 90 206 114 887.3 0 114 207 70 887.3 0 70 208 94 887.3 0 94 209 97 887.3 0 97 210 107 887.3 0 107 211 142 887.3 0 142 212 109 887.3 0 109 213 108 887.3 0 108 214 100 887.3 0 100 215 131 887.3 0 131 216 166 887.3 0 166 217 140 887.3 0 140 218 87 887.3 0 87 219 91 887.3 0 91 220 84 887.3 0 84 221 207 887.3 0 207 222 254 887.3 0 254 223 155 887.3 0 155 224 675 887.3 0 675 225 77 887.3 0 77 226 79 887.3 0 79 227 73 887.3 0 73 228 102 887.3 0 102 229 79 887.3 0 79 230 132 887.3 0 132 231 182 887.3 0 182 232 165 887.3 0 165 233 135 887.3 0 135 234 126 887.3 0 126 235 180 887.3 0 180 236 362 887.3 0 362 237 457 887.3 0 457 238 375 887.3 0 375 239 83 887.3 0 83 240 118 887.3 0 118 241 113 887.3 0 113 242 143 887.3 0 143 243 84 887.3 0 84 244 77 887.3 0 77 245 63 887.3 0 63 246 100 887.3 0 100 247 78 887.3 0 78 248 70 887.3 0 70 249 78 887.3 0 78 250 90 887.3 0 90 251 74 887.3 0 74 252 81 887.3 0 81 253 166 887.3 0 166 254 134 887.3 0 134 255 130 887.3 0 130 256 98 887.3 0 98 257 80 887.3 0 80 258 78 887.3 0 78 259 69 887.3 0 69 260 89 887.3 0 89 261 150 887.3 0 150 262 208 887.3 0 208 263 272 887.3 0 272 264 134 887.3 0 134 265 81 887.3 0 81 266 91 887.3 0 91 267 95 887.3 0 95 268 82 887.3 0 82 269 87 887.3 0 87 270 131 887.3 0 131 271 490 887.3 0 490 272 750 887.3 0 750 273 621 887.3 0 621 274 216 887.3 0 216 275 80 887.3 0 80 276 90 887.3 0 90 277 103 887.3 0 103 278 104 887.3 0 104 279 221 887.3 0 221 280 194 887.3 0 194 281 116 887.3 0 116 282 115 887.3 0 115 283 135 887.3 0 135 284 131 887.3 0 131 285 128 887.3 0 128 286 115 887.3 0 115 287 100 887.3 0 100 288 111 887.3 0 111 289 104 887.3 0 104 290 172 887.3 0 172 291 107 887.3 0 107 292 289 887.3 0 289 293 296 887.3 0 296 294 195 887.3 0 195 295 116 887.3 0 116 296 466 887.3 0 466 297 453 887.3 0 453 298 329 887.3 0 329 299 175 887.3 0 175 300 134 887.3 0 134 301 68 887.3 0 68 Finished in 16.80 s (66 us/read; 0.91 M reads/minute). === Summary === Total reads processed: 253,654 Reads with adapters: 77,378 (30.5%) Reads that were too short: 252 (0.1%) Reads written (passing filters): 77,126 (30.4%) Total basepairs processed: 57,772,802 bp Total written (filtered): 21,354,905 bp (37.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 77378 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 36 0.0 3 0 0 26 10 16 701 0.0 3 0 611 59 31 17 75851 0.0 3 72060 3157 497 137 18 212 0.0 3 12 188 8 4 19 173 0.0 3 1 0 112 60 20 71 0.0 3 1 1 0 69 21 2 0.0 3 0 0 0 2 22 2 0.0 3 2 23 2 0.0 3 1 1 25 1 0.0 3 0 1 26 1 0.0 3 0 0 0 1 27 1 0.0 3 1 30 2 0.0 3 0 0 0 2 31 7 0.0 3 2 0 0 5 32 1 0.0 3 0 0 0 1 37 1 0.0 3 1 46 1 0.0 3 0 0 0 1 47 6 0.0 3 0 0 0 6 48 5 0.0 3 0 0 2 3 49 5 0.0 3 0 0 0 5 50 4 0.0 3 0 0 0 4 53 4 0.0 3 0 0 0 4 59 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 62 4 0.0 3 0 0 0 4 63 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 76 3 0.0 3 0 0 0 3 81 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 98 3 0.0 3 0 0 0 3 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 102 4 0.0 3 0 0 0 4 103 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 0 2 117 1 0.0 3 0 0 0 1 119 2 0.0 3 0 0 0 2 120 1 0.0 3 0 0 0 1 122 2 0.0 3 0 0 0 2 124 1 0.0 3 0 0 0 1 129 3 0.0 3 0 0 0 3 131 1 0.0 3 0 0 0 1 132 7 0.0 3 0 0 0 7 134 1 0.0 3 0 0 0 1 141 2 0.0 3 0 0 0 2 146 2 0.0 3 0 0 0 2 148 1 0.0 3 0 0 0 1 149 2 0.0 3 0 0 0 2 150 5 0.0 3 0 0 0 5 151 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 162 2 0.0 3 0 0 0 2 163 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 184 3 0.0 3 0 0 0 3 188 1 0.0 3 0 0 0 1 192 3 0.0 3 0 0 0 3 194 2 0.0 3 0 0 0 2 203 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 205 3 0.0 3 0 0 0 3 208 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 216 4 0.0 3 0 0 0 4 217 154 0.0 3 0 0 13 141 218 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 221 4 0.0 3 0 0 0 4 230 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 259 1 0.0 3 0 0 0 1 260 1 0.0 3 0 0 0 1 267 1 0.0 3 0 0 0 1 270 2 0.0 3 0 0 0 2 275 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 4 0.0 3 0 0 0 4 280 2 0.0 3 0 0 0 2 281 8 0.0 3 0 0 1 7 282 2 0.0 3 0 0 0 2 284 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 295 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.55 s (59 us/read; 1.02 M reads/minute). === Summary === Total reads processed: 77,126 Reads with adapters: 1,365 (1.8%) Reads written (passing filters): 77,126 (100.0%) Total basepairs processed: 21,354,905 bp Total written (filtered): 21,350,790 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1365 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 13.3% C: 74.9% G: 10.3% T: 1.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1350 1205.1 0 1350 4 12 301.3 0 12 5 1 75.3 0 1 6 2 18.8 0 2