This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 171.41 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 96,020 (2.6%) Reads that were too short: 10,422 (0.3%) Reads written (passing filters): 85,598 (2.4%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 23,534,304 bp (2.2%) === Adapter 1 === Sequence: GGTCGA; Type: regular 5'; Length: 6; Trimmed: 96020 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 9418 887.3 0 9418 7 13146 887.3 0 13146 8 28860 887.3 0 28860 9 19683 887.3 0 19683 10 56 887.3 0 56 11 106 887.3 0 106 12 28 887.3 0 28 13 43 887.3 0 43 14 29 887.3 0 29 15 35 887.3 0 35 16 29 887.3 0 29 17 13 887.3 0 13 18 27 887.3 0 27 19 18 887.3 0 18 20 22 887.3 0 22 21 30 887.3 0 30 22 51 887.3 0 51 23 26 887.3 0 26 24 35 887.3 0 35 25 24 887.3 0 24 26 16 887.3 0 16 27 42 887.3 0 42 28 21 887.3 0 21 29 20 887.3 0 20 30 36 887.3 0 36 31 19 887.3 0 19 32 16 887.3 0 16 33 18 887.3 0 18 34 24 887.3 0 24 35 48 887.3 0 48 36 13 887.3 0 13 37 38 887.3 0 38 38 65 887.3 0 65 39 27 887.3 0 27 40 36 887.3 0 36 41 42 887.3 0 42 42 24 887.3 0 24 43 31 887.3 0 31 44 27 887.3 0 27 45 48 887.3 0 48 46 38 887.3 0 38 47 103 887.3 0 103 48 570 887.3 0 570 49 818 887.3 0 818 50 1005 887.3 0 1005 51 54 887.3 0 54 52 22 887.3 0 22 53 27 887.3 0 27 54 23 887.3 0 23 55 17 887.3 0 17 56 23 887.3 0 23 57 29 887.3 0 29 58 28 887.3 0 28 59 30 887.3 0 30 60 31 887.3 0 31 61 27 887.3 0 27 62 19 887.3 0 19 63 17 887.3 0 17 64 17 887.3 0 17 65 18 887.3 0 18 66 19 887.3 0 19 67 25 887.3 0 25 68 29 887.3 0 29 69 20 887.3 0 20 70 39 887.3 0 39 71 25 887.3 0 25 72 14 887.3 0 14 73 19 887.3 0 19 74 32 887.3 0 32 75 37 887.3 0 37 76 57 887.3 0 57 77 32 887.3 0 32 78 25 887.3 0 25 79 27 887.3 0 27 80 63 887.3 0 63 81 158 887.3 0 158 82 199 887.3 0 199 83 517 887.3 0 517 84 37 887.3 0 37 85 36 887.3 0 36 86 46 887.3 0 46 87 29 887.3 0 29 88 21 887.3 0 21 89 301 887.3 0 301 90 63 887.3 0 63 91 42 887.3 0 42 92 74 887.3 0 74 93 52 887.3 0 52 94 24 887.3 0 24 95 33 887.3 0 33 96 89 887.3 0 89 97 155 887.3 0 155 98 475 887.3 0 475 99 27 887.3 0 27 100 23 887.3 0 23 101 15 887.3 0 15 102 11 887.3 0 11 103 26 887.3 0 26 104 34 887.3 0 34 105 23 887.3 0 23 106 33 887.3 0 33 107 14 887.3 0 14 108 28 887.3 0 28 109 24 887.3 0 24 110 26 887.3 0 26 111 15 887.3 0 15 112 34 887.3 0 34 113 191 887.3 0 191 114 51 887.3 0 51 115 185 887.3 0 185 116 30 887.3 0 30 117 49 887.3 0 49 118 116 887.3 0 116 119 74 887.3 0 74 120 102 887.3 0 102 121 29 887.3 0 29 122 45 887.3 0 45 123 25 887.3 0 25 124 20 887.3 0 20 125 23 887.3 0 23 126 20 887.3 0 20 127 47 887.3 0 47 128 25 887.3 0 25 129 35 887.3 0 35 130 41 887.3 0 41 131 25 887.3 0 25 132 36 887.3 0 36 133 39 887.3 0 39 134 31 887.3 0 31 135 32 887.3 0 32 136 170 887.3 0 170 137 116 887.3 0 116 138 85 887.3 0 85 139 22 887.3 0 22 140 24 887.3 0 24 141 31 887.3 0 31 142 54 887.3 0 54 143 44 887.3 0 44 144 50 887.3 0 50 145 84 887.3 0 84 146 57 887.3 0 57 147 141 887.3 0 141 148 79 887.3 0 79 149 50 887.3 0 50 150 32 887.3 0 32 151 32 887.3 0 32 152 61 887.3 0 61 153 56 887.3 0 56 154 65 887.3 0 65 155 106 887.3 0 106 156 45 887.3 0 45 157 164 887.3 0 164 158 104 887.3 0 104 159 27 887.3 0 27 160 18 887.3 0 18 161 23 887.3 0 23 162 36 887.3 0 36 163 28 887.3 0 28 164 35 887.3 0 35 165 27 887.3 0 27 166 18 887.3 0 18 167 20 887.3 0 20 168 43 887.3 0 43 169 41 887.3 0 41 170 35 887.3 0 35 171 33 887.3 0 33 172 45 887.3 0 45 173 26 887.3 0 26 174 24 887.3 0 24 175 24 887.3 0 24 176 34 887.3 0 34 177 32 887.3 0 32 178 55 887.3 0 55 179 35 887.3 0 35 180 38 887.3 0 38 181 98 887.3 0 98 182 71 887.3 0 71 183 56 887.3 0 56 184 20 887.3 0 20 185 35 887.3 0 35 186 30 887.3 0 30 187 37 887.3 0 37 188 36 887.3 0 36 189 66 887.3 0 66 190 87 887.3 0 87 191 186 887.3 0 186 192 68 887.3 0 68 193 184 887.3 0 184 194 259 887.3 0 259 195 717 887.3 0 717 196 941 887.3 0 941 197 584 887.3 0 584 198 35 887.3 0 35 199 34 887.3 0 34 200 21 887.3 0 21 201 40 887.3 0 40 202 31 887.3 0 31 203 25 887.3 0 25 204 28 887.3 0 28 205 30 887.3 0 30 206 23 887.3 0 23 207 28 887.3 0 28 208 30 887.3 0 30 209 51 887.3 0 51 210 30 887.3 0 30 211 33 887.3 0 33 212 30 887.3 0 30 213 53 887.3 0 53 214 89 887.3 0 89 215 116 887.3 0 116 216 92 887.3 0 92 217 50 887.3 0 50 218 55 887.3 0 55 219 47 887.3 0 47 220 34 887.3 0 34 221 53 887.3 0 53 222 34 887.3 0 34 223 39 887.3 0 39 224 29 887.3 0 29 225 43 887.3 0 43 226 39 887.3 0 39 227 22 887.3 0 22 228 18 887.3 0 18 229 63 887.3 0 63 230 59 887.3 0 59 231 62 887.3 0 62 232 45 887.3 0 45 233 50 887.3 0 50 234 63 887.3 0 63 235 96 887.3 0 96 236 127 887.3 0 127 237 46 887.3 0 46 238 79 887.3 0 79 239 77 887.3 0 77 240 119 887.3 0 119 241 134 887.3 0 134 242 89 887.3 0 89 243 453 887.3 0 453 244 351 887.3 0 351 245 398 887.3 0 398 246 68 887.3 0 68 247 73 887.3 0 73 248 76 887.3 0 76 249 114 887.3 0 114 250 96 887.3 0 96 251 131 887.3 0 131 252 210 887.3 0 210 253 169 887.3 0 169 254 138 887.3 0 138 255 90 887.3 0 90 256 105 887.3 0 105 257 81 887.3 0 81 258 63 887.3 0 63 259 181 887.3 0 181 260 130 887.3 0 130 261 222 887.3 0 222 262 211 887.3 0 211 263 144 887.3 0 144 264 68 887.3 0 68 265 66 887.3 0 66 266 63 887.3 0 63 267 43 887.3 0 43 268 77 887.3 0 77 269 170 887.3 0 170 270 152 887.3 0 152 271 115 887.3 0 115 272 50 887.3 0 50 273 44 887.3 0 44 274 45 887.3 0 45 275 50 887.3 0 50 276 41 887.3 0 41 277 54 887.3 0 54 278 58 887.3 0 58 279 56 887.3 0 56 280 46 887.3 0 46 281 71 887.3 0 71 282 99 887.3 0 99 283 80 887.3 0 80 284 146 887.3 0 146 285 110 887.3 0 110 286 175 887.3 0 175 287 132 887.3 0 132 288 144 887.3 0 144 289 244 887.3 0 244 290 127 887.3 0 127 291 230 887.3 0 230 292 201 887.3 0 201 293 116 887.3 0 116 294 108 887.3 0 108 295 117 887.3 0 117 296 127 887.3 0 127 297 400 887.3 0 400 298 322 887.3 0 322 299 281 887.3 0 281 300 158 887.3 0 158 301 71 887.3 0 71 Finished in 6.40 s (75 us/read; 0.80 M reads/minute). === Summary === Total reads processed: 85,598 Reads with adapters: 36,310 (42.4%) Reads that were too short: 246 (0.3%) Reads written (passing filters): 36,064 (42.1%) Total basepairs processed: 23,534,304 bp Total written (filtered): 9,932,772 bp (42.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 36310 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 13 0.0 3 0 0 9 4 16 245 0.0 3 0 216 15 14 17 26940 0.0 3 25637 1103 160 40 18 154 0.0 3 6 136 8 4 19 8483 0.0 3 7974 378 100 31 20 54 0.0 3 10 15 0 29 21 16 0.0 3 0 0 8 8 22 11 0.0 3 1 0 0 10 28 1 0.0 3 0 0 1 29 14 0.0 3 0 0 1 13 31 6 0.0 3 0 0 0 6 32 2 0.0 3 1 0 0 1 35 9 0.0 3 0 0 0 9 38 1 0.0 3 0 0 0 1 41 2 0.0 3 0 0 0 2 42 1 0.0 3 0 0 0 1 45 2 0.0 3 0 0 0 2 48 1 0.0 3 0 0 0 1 53 6 0.0 3 0 0 0 6 55 2 0.0 3 0 0 0 2 56 1 0.0 3 0 0 0 1 60 2 0.0 3 0 0 0 2 61 3 0.0 3 0 0 1 2 62 1 0.0 3 0 0 0 1 72 2 0.0 3 0 0 0 2 75 1 0.0 3 0 0 0 1 79 19 0.0 3 0 0 0 19 82 1 0.0 3 0 0 1 83 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 90 2 0.0 3 0 0 0 2 91 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 4 0.0 3 0 0 0 4 103 1 0.0 3 0 0 0 1 105 2 0.0 3 0 0 0 2 106 2 0.0 3 0 0 0 2 111 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 2 0.0 3 0 0 1 1 122 1 0.0 3 0 0 0 1 123 16 0.0 3 0 0 0 16 126 3 0.0 3 0 0 0 3 135 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 138 3 0.0 3 0 0 0 3 139 1 0.0 3 0 0 0 1 141 2 0.0 3 0 0 0 2 142 2 0.0 3 0 0 0 2 143 13 0.0 3 0 0 0 13 144 5 0.0 3 0 0 0 5 145 1 0.0 3 0 0 0 1 146 3 0.0 3 0 0 0 3 147 3 0.0 3 0 0 0 3 148 1 0.0 3 0 0 1 149 5 0.0 3 0 0 0 5 155 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 165 2 0.0 3 0 0 0 2 167 1 0.0 3 0 0 0 1 168 2 0.0 3 0 0 0 2 169 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 184 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 195 3 0.0 3 0 0 0 3 203 1 0.0 3 0 0 0 1 204 3 0.0 3 0 0 0 3 207 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 216 7 0.0 3 0 0 0 7 217 159 0.0 3 0 0 6 153 218 3 0.0 3 0 0 0 3 221 2 0.0 3 0 0 1 1 223 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 235 2 0.0 3 0 0 0 2 236 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 245 2 0.0 3 0 0 0 2 256 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 8 0.0 3 0 0 0 8 283 4 0.0 3 0 0 0 4 285 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.08 s (58 us/read; 1.04 M reads/minute). === Summary === Total reads processed: 36,064 Reads with adapters: 149 (0.4%) Reads written (passing filters): 36,064 (100.0%) Total basepairs processed: 9,932,772 bp Total written (filtered): 9,932,300 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 149 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 56.4% C: 18.8% G: 18.8% T: 6.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 126 563.5 0 126 4 21 140.9 0 21 5 2 35.2 0 2