This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 171.77 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 86,473 (2.4%) Reads that were too short: 4,824 (0.1%) Reads written (passing filters): 81,649 (2.2%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 23,090,496 bp (2.1%) === Adapter 1 === Sequence: GGTCGA; Type: regular 5'; Length: 6; Trimmed: 86473 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 8667 887.3 0 8667 7 15034 887.3 0 15034 8 24565 887.3 0 24565 9 25971 887.3 0 25971 10 58 887.3 0 58 11 27 887.3 0 27 12 11 887.3 0 11 13 14 887.3 0 14 14 13 887.3 0 13 15 14 887.3 0 14 16 13 887.3 0 13 17 4 887.3 0 4 18 15 887.3 0 15 19 14 887.3 0 14 20 8 887.3 0 8 21 18 887.3 0 18 22 15 887.3 0 15 23 13 887.3 0 13 24 9 887.3 0 9 25 15 887.3 0 15 26 7 887.3 0 7 27 18 887.3 0 18 28 13 887.3 0 13 29 9 887.3 0 9 30 22 887.3 0 22 31 16 887.3 0 16 32 10 887.3 0 10 33 9 887.3 0 9 34 11 887.3 0 11 35 15 887.3 0 15 36 6 887.3 0 6 37 24 887.3 0 24 38 23 887.3 0 23 39 11 887.3 0 11 40 20 887.3 0 20 41 27 887.3 0 27 42 23 887.3 0 23 43 23 887.3 0 23 44 8 887.3 0 8 45 17 887.3 0 17 46 20 887.3 0 20 47 13 887.3 0 13 48 238 887.3 0 238 49 355 887.3 0 355 50 405 887.3 0 405 51 15 887.3 0 15 52 16 887.3 0 16 53 16 887.3 0 16 54 15 887.3 0 15 55 9 887.3 0 9 56 16 887.3 0 16 57 19 887.3 0 19 58 14 887.3 0 14 59 15 887.3 0 15 60 12 887.3 0 12 61 15 887.3 0 15 62 17 887.3 0 17 63 13 887.3 0 13 64 17 887.3 0 17 65 45 887.3 0 45 66 14 887.3 0 14 67 20 887.3 0 20 68 26 887.3 0 26 69 13 887.3 0 13 70 30 887.3 0 30 71 11 887.3 0 11 72 16 887.3 0 16 73 15 887.3 0 15 74 22 887.3 0 22 75 13 887.3 0 13 76 9 887.3 0 9 77 10 887.3 0 10 78 21 887.3 0 21 79 19 887.3 0 19 80 21 887.3 0 21 81 49 887.3 0 49 82 39 887.3 0 39 83 177 887.3 0 177 84 49 887.3 0 49 85 27 887.3 0 27 86 25 887.3 0 25 87 19 887.3 0 19 88 17 887.3 0 17 89 23 887.3 0 23 90 25 887.3 0 25 91 19 887.3 0 19 92 20 887.3 0 20 93 28 887.3 0 28 94 20 887.3 0 20 95 17 887.3 0 17 96 33 887.3 0 33 97 60 887.3 0 60 98 386 887.3 0 386 99 23 887.3 0 23 100 17 887.3 0 17 101 13 887.3 0 13 102 11 887.3 0 11 103 11 887.3 0 11 104 22 887.3 0 22 105 21 887.3 0 21 106 16 887.3 0 16 107 19 887.3 0 19 108 14 887.3 0 14 109 24 887.3 0 24 110 17 887.3 0 17 111 10 887.3 0 10 112 16 887.3 0 16 113 50 887.3 0 50 114 26 887.3 0 26 115 60 887.3 0 60 116 17 887.3 0 17 117 16 887.3 0 16 118 64 887.3 0 64 119 43 887.3 0 43 120 34 887.3 0 34 121 25 887.3 0 25 122 24 887.3 0 24 123 18 887.3 0 18 124 15 887.3 0 15 125 18 887.3 0 18 126 13 887.3 0 13 127 30 887.3 0 30 128 20 887.3 0 20 129 29 887.3 0 29 130 18 887.3 0 18 131 17 887.3 0 17 132 22 887.3 0 22 133 15 887.3 0 15 134 18 887.3 0 18 135 18 887.3 0 18 136 60 887.3 0 60 137 45 887.3 0 45 138 31 887.3 0 31 139 15 887.3 0 15 140 11 887.3 0 11 141 40 887.3 0 40 142 30 887.3 0 30 143 17 887.3 0 17 144 20 887.3 0 20 145 30 887.3 0 30 146 33 887.3 0 33 147 154 887.3 0 154 148 50 887.3 0 50 149 24 887.3 0 24 150 30 887.3 0 30 151 20 887.3 0 20 152 67 887.3 0 67 153 37 887.3 0 37 154 50 887.3 0 50 155 83 887.3 0 83 156 32 887.3 0 32 157 73 887.3 0 73 158 38 887.3 0 38 159 17 887.3 0 17 160 7 887.3 0 7 161 20 887.3 0 20 162 20 887.3 0 20 163 19 887.3 0 19 164 21 887.3 0 21 165 22 887.3 0 22 166 12 887.3 0 12 167 24 887.3 0 24 168 25 887.3 0 25 169 14 887.3 0 14 170 22 887.3 0 22 171 13 887.3 0 13 172 18 887.3 0 18 173 17 887.3 0 17 174 11 887.3 0 11 175 23 887.3 0 23 176 24 887.3 0 24 177 26 887.3 0 26 178 26 887.3 0 26 179 32 887.3 0 32 180 27 887.3 0 27 181 101 887.3 0 101 182 70 887.3 0 70 183 54 887.3 0 54 184 17 887.3 0 17 185 24 887.3 0 24 186 27 887.3 0 27 187 17 887.3 0 17 188 21 887.3 0 21 189 38 887.3 0 38 190 36 887.3 0 36 191 161 887.3 0 161 192 51 887.3 0 51 193 169 887.3 0 169 194 79 887.3 0 79 195 320 887.3 0 320 196 413 887.3 0 413 197 315 887.3 0 315 198 17 887.3 0 17 199 16 887.3 0 16 200 18 887.3 0 18 201 23 887.3 0 23 202 21 887.3 0 21 203 19 887.3 0 19 204 10 887.3 0 10 205 11 887.3 0 11 206 10 887.3 0 10 207 25 887.3 0 25 208 17 887.3 0 17 209 33 887.3 0 33 210 16 887.3 0 16 211 7 887.3 0 7 212 10 887.3 0 10 213 32 887.3 0 32 214 27 887.3 0 27 215 51 887.3 0 51 216 58 887.3 0 58 217 22 887.3 0 22 218 30 887.3 0 30 219 20 887.3 0 20 220 12 887.3 0 12 221 20 887.3 0 20 222 18 887.3 0 18 223 17 887.3 0 17 224 19 887.3 0 19 225 14 887.3 0 14 226 20 887.3 0 20 227 19 887.3 0 19 228 22 887.3 0 22 229 17 887.3 0 17 230 25 887.3 0 25 231 33 887.3 0 33 232 24 887.3 0 24 233 24 887.3 0 24 234 43 887.3 0 43 235 76 887.3 0 76 236 108 887.3 0 108 237 27 887.3 0 27 238 37 887.3 0 37 239 30 887.3 0 30 240 45 887.3 0 45 241 45 887.3 0 45 242 28 887.3 0 28 243 129 887.3 0 129 244 108 887.3 0 108 245 115 887.3 0 115 246 30 887.3 0 30 247 38 887.3 0 38 248 48 887.3 0 48 249 59 887.3 0 59 250 29 887.3 0 29 251 85 887.3 0 85 252 49 887.3 0 49 253 60 887.3 0 60 254 44 887.3 0 44 255 33 887.3 0 33 256 62 887.3 0 62 257 38 887.3 0 38 258 29 887.3 0 29 259 78 887.3 0 78 260 57 887.3 0 57 261 92 887.3 0 92 262 72 887.3 0 72 263 68 887.3 0 68 264 30 887.3 0 30 265 30 887.3 0 30 266 11 887.3 0 11 267 22 887.3 0 22 268 27 887.3 0 27 269 43 887.3 0 43 270 47 887.3 0 47 271 43 887.3 0 43 272 27 887.3 0 27 273 21 887.3 0 21 274 21 887.3 0 21 275 31 887.3 0 31 276 28 887.3 0 28 277 23 887.3 0 23 278 31 887.3 0 31 279 21 887.3 0 21 280 24 887.3 0 24 281 38 887.3 0 38 282 46 887.3 0 46 283 29 887.3 0 29 284 54 887.3 0 54 285 39 887.3 0 39 286 91 887.3 0 91 287 77 887.3 0 77 288 96 887.3 0 96 289 204 887.3 0 204 290 71 887.3 0 71 291 131 887.3 0 131 292 182 887.3 0 182 293 44 887.3 0 44 294 47 887.3 0 47 295 42 887.3 0 42 296 65 887.3 0 65 297 144 887.3 0 144 298 155 887.3 0 155 299 151 887.3 0 151 300 128 887.3 0 128 301 45 887.3 0 45 Finished in 6.62 s (81 us/read; 0.74 M reads/minute). === Summary === Total reads processed: 81,649 Reads with adapters: 43,498 (53.3%) Reads that were too short: 13 (0.0%) Reads written (passing filters): 43,485 (53.3%) Total basepairs processed: 23,090,496 bp Total written (filtered): 11,772,008 bp (51.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 43498 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 8 0.0 3 0 0 8 19 478 0.0 3 0 461 10 4 3 20 27645 0.0 4 27202 421 20 1 1 21 233 0.0 4 24 203 4 1 1 22 14491 0.0 4 14221 254 13 2 1 23 55 0.0 4 7 27 4 15 2 24 4 0.0 4 0 1 2 0 1 25 10 0.0 4 0 0 0 10 26 1 0.0 4 0 0 0 0 1 31 1 0.0 4 1 86 6 0.0 4 0 0 0 0 6 117 1 0.0 4 1 126 3 0.0 4 0 0 0 0 3 127 400 0.0 4 0 0 0 0 400 128 5 0.0 4 0 0 0 0 5 129 145 0.0 4 0 0 0 0 145 156 1 0.0 4 0 0 0 0 1 182 1 0.0 4 1 187 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 229 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 246 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.57 s (59 us/read; 1.02 M reads/minute). === Summary === Total reads processed: 43,485 Reads with adapters: 4,927 (11.3%) Reads written (passing filters): 43,485 (100.0%) Total basepairs processed: 11,772,008 bp Total written (filtered): 11,719,817 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4927 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.5% C: 2.5% G: 95.4% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 8 679.5 0 8 4 8 169.9 0 8 5 2 42.5 0 2 6 17 10.6 0 17 7 1 2.7 0 1 8 2 0.7 0 2 9 2257 0.2 0 53 2204 10 2249 0.0 1 67 2182 11 344 0.0 1 1 343 12 6 0.0 1 0 6 13 3 0.0 1 1 2 15 1 0.0 1 0 1 82 1 0.0 1 1 112 1 0.0 1 1 150 12 0.0 1 0 12 169 1 0.0 1 1 223 12 0.0 1 11 1 224 2 0.0 1 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.