This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGCGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 174.61 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 173,241 (4.8%) Reads that were too short: 52,275 (1.4%) Reads written (passing filters): 120,966 (3.3%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 31,400,081 bp (2.9%) === Adapter 1 === Sequence: TGCGTG; Type: regular 5'; Length: 6; Trimmed: 173241 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2812 887.3 0 2812 7 44271 887.3 0 44271 8 35170 887.3 0 35170 9 118 887.3 0 118 10 334 887.3 0 334 11 63 887.3 0 63 12 56 887.3 0 56 13 51 887.3 0 51 14 62 887.3 0 62 15 86 887.3 0 86 16 62 887.3 0 62 17 89 887.3 0 89 18 87 887.3 0 87 19 194 887.3 0 194 20 50 887.3 0 50 21 65 887.3 0 65 22 42 887.3 0 42 23 58 887.3 0 58 24 54 887.3 0 54 25 57 887.3 0 57 26 70 887.3 0 70 27 56 887.3 0 56 28 67 887.3 0 67 29 74 887.3 0 74 30 67 887.3 0 67 31 67 887.3 0 67 32 50 887.3 0 50 33 75 887.3 0 75 34 63 887.3 0 63 35 69 887.3 0 69 36 71 887.3 0 71 37 65 887.3 0 65 38 47 887.3 0 47 39 67 887.3 0 67 40 77 887.3 0 77 41 52 887.3 0 52 42 88 887.3 0 88 43 77 887.3 0 77 44 48 887.3 0 48 45 39 887.3 0 39 46 89 887.3 0 89 47 97 887.3 0 97 48 83 887.3 0 83 49 129 887.3 0 129 50 70 887.3 0 70 51 55 887.3 0 55 52 76 887.3 0 76 53 164 887.3 0 164 54 88 887.3 0 88 55 78 887.3 0 78 56 86 887.3 0 86 57 133 887.3 0 133 58 61 887.3 0 61 59 61 887.3 0 61 60 129 887.3 0 129 61 89 887.3 0 89 62 115 887.3 0 115 63 86 887.3 0 86 64 100 887.3 0 100 65 65 887.3 0 65 66 74 887.3 0 74 67 83 887.3 0 83 68 87 887.3 0 87 69 77 887.3 0 77 70 103 887.3 0 103 71 136 887.3 0 136 72 171 887.3 0 171 73 1014 887.3 0 1014 74 1023 887.3 0 1023 75 872 887.3 0 872 76 295 887.3 0 295 77 2606 887.3 0 2606 78 3088 887.3 0 3088 79 912 887.3 0 912 80 66 887.3 0 66 81 46 887.3 0 46 82 65 887.3 0 65 83 64 887.3 0 64 84 85 887.3 0 85 85 75 887.3 0 75 86 111 887.3 0 111 87 69 887.3 0 69 88 136 887.3 0 136 89 86 887.3 0 86 90 83 887.3 0 83 91 75 887.3 0 75 92 85 887.3 0 85 93 108 887.3 0 108 94 83 887.3 0 83 95 72 887.3 0 72 96 68 887.3 0 68 97 76 887.3 0 76 98 63 887.3 0 63 99 112 887.3 0 112 100 78 887.3 0 78 101 60 887.3 0 60 102 46 887.3 0 46 103 58 887.3 0 58 104 82 887.3 0 82 105 77 887.3 0 77 106 250 887.3 0 250 107 362 887.3 0 362 108 498 887.3 0 498 109 66 887.3 0 66 110 59 887.3 0 59 111 69 887.3 0 69 112 103 887.3 0 103 113 99 887.3 0 99 114 86 887.3 0 86 115 158 887.3 0 158 116 90 887.3 0 90 117 75 887.3 0 75 118 79 887.3 0 79 119 74 887.3 0 74 120 95 887.3 0 95 121 71 887.3 0 71 122 84 887.3 0 84 123 146 887.3 0 146 124 130 887.3 0 130 125 110 887.3 0 110 126 113 887.3 0 113 127 201 887.3 0 201 128 1021 887.3 0 1021 129 658 887.3 0 658 130 1405 887.3 0 1405 131 115 887.3 0 115 132 101 887.3 0 101 133 207 887.3 0 207 134 744 887.3 0 744 135 392 887.3 0 392 136 507 887.3 0 507 137 231 887.3 0 231 138 138 887.3 0 138 139 101 887.3 0 101 140 114 887.3 0 114 141 97 887.3 0 97 142 118 887.3 0 118 143 89 887.3 0 89 144 113 887.3 0 113 145 217 887.3 0 217 146 1154 887.3 0 1154 147 1398 887.3 0 1398 148 719 887.3 0 719 149 173 887.3 0 173 150 75 887.3 0 75 151 66 887.3 0 66 152 75 887.3 0 75 153 81 887.3 0 81 154 94 887.3 0 94 155 251 887.3 0 251 156 312 887.3 0 312 157 481 887.3 0 481 158 167 887.3 0 167 159 161 887.3 0 161 160 128 887.3 0 128 161 137 887.3 0 137 162 100 887.3 0 100 163 72 887.3 0 72 164 220 887.3 0 220 165 268 887.3 0 268 166 257 887.3 0 257 167 104 887.3 0 104 168 78 887.3 0 78 169 96 887.3 0 96 170 79 887.3 0 79 171 77 887.3 0 77 172 66 887.3 0 66 173 51 887.3 0 51 174 65 887.3 0 65 175 77 887.3 0 77 176 49 887.3 0 49 177 64 887.3 0 64 178 81 887.3 0 81 179 121 887.3 0 121 180 97 887.3 0 97 181 103 887.3 0 103 182 83 887.3 0 83 183 94 887.3 0 94 184 260 887.3 0 260 185 333 887.3 0 333 186 354 887.3 0 354 187 154 887.3 0 154 188 99 887.3 0 99 189 75 887.3 0 75 190 81 887.3 0 81 191 112 887.3 0 112 192 133 887.3 0 133 193 114 887.3 0 114 194 119 887.3 0 119 195 960 887.3 0 960 196 710 887.3 0 710 197 437 887.3 0 437 198 91 887.3 0 91 199 115 887.3 0 115 200 87 887.3 0 87 201 78 887.3 0 78 202 66 887.3 0 66 203 96 887.3 0 96 204 103 887.3 0 103 205 81 887.3 0 81 206 101 887.3 0 101 207 81 887.3 0 81 208 79 887.3 0 79 209 109 887.3 0 109 210 106 887.3 0 106 211 108 887.3 0 108 212 149 887.3 0 149 213 120 887.3 0 120 214 127 887.3 0 127 215 344 887.3 0 344 216 358 887.3 0 358 217 230 887.3 0 230 218 171 887.3 0 171 219 182 887.3 0 182 220 128 887.3 0 128 221 116 887.3 0 116 222 71 887.3 0 71 223 182 887.3 0 182 224 115 887.3 0 115 225 138 887.3 0 138 226 123 887.3 0 123 227 119 887.3 0 119 228 75 887.3 0 75 229 103 887.3 0 103 230 128 887.3 0 128 231 177 887.3 0 177 232 180 887.3 0 180 233 322 887.3 0 322 234 355 887.3 0 355 235 539 887.3 0 539 236 5259 887.3 0 5259 237 12186 887.3 0 12186 238 12314 887.3 0 12314 239 212 887.3 0 212 240 131 887.3 0 131 241 128 887.3 0 128 242 111 887.3 0 111 243 90 887.3 0 90 244 112 887.3 0 112 245 164 887.3 0 164 246 438 887.3 0 438 247 499 887.3 0 499 248 412 887.3 0 412 249 253 887.3 0 253 250 262 887.3 0 262 251 478 887.3 0 478 252 422 887.3 0 422 253 611 887.3 0 611 254 381 887.3 0 381 255 267 887.3 0 267 256 108 887.3 0 108 257 109 887.3 0 109 258 107 887.3 0 107 259 107 887.3 0 107 260 81 887.3 0 81 261 80 887.3 0 80 262 103 887.3 0 103 263 165 887.3 0 165 264 148 887.3 0 148 265 131 887.3 0 131 266 121 887.3 0 121 267 107 887.3 0 107 268 98 887.3 0 98 269 104 887.3 0 104 270 112 887.3 0 112 271 111 887.3 0 111 272 103 887.3 0 103 273 122 887.3 0 122 274 137 887.3 0 137 275 81 887.3 0 81 276 96 887.3 0 96 277 148 887.3 0 148 278 211 887.3 0 211 279 156 887.3 0 156 280 257 887.3 0 257 281 303 887.3 0 303 282 470 887.3 0 470 283 355 887.3 0 355 284 766 887.3 0 766 285 745 887.3 0 745 286 554 887.3 0 554 287 142 887.3 0 142 288 127 887.3 0 127 289 195 887.3 0 195 290 148 887.3 0 148 291 139 887.3 0 139 292 113 887.3 0 113 293 262 887.3 0 262 294 319 887.3 0 319 295 470 887.3 0 470 296 752 887.3 0 752 297 1047 887.3 0 1047 298 924 887.3 0 924 299 368 887.3 0 368 300 369 887.3 0 369 301 392 887.3 0 392 Finished in 8.77 s (72 us/read; 0.83 M reads/minute). === Summary === Total reads processed: 120,966 Reads with adapters: 46,148 (38.1%) Reads that were too short: 313 (0.3%) Reads written (passing filters): 45,835 (37.9%) Total basepairs processed: 31,400,081 bp Total written (filtered): 12,626,922 bp (40.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 46148 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 6 0.0 2 0 0 0 6 15 30 0.0 3 0 0 20 10 16 387 0.0 3 0 339 28 20 17 44647 0.0 3 42137 2050 365 95 18 134 0.0 3 26 97 9 2 19 120 0.0 3 0 0 67 53 20 59 0.0 3 0 0 1 58 23 3 0.0 3 0 1 0 2 26 1 0.0 3 0 0 0 1 27 4 0.0 3 0 0 0 4 28 1 0.0 3 0 0 0 1 29 1 0.0 3 1 31 1 0.0 3 0 0 0 1 33 4 0.0 3 0 0 0 4 38 1 0.0 3 0 0 0 1 39 72 0.0 3 0 0 1 71 42 5 0.0 3 0 0 0 5 43 2 0.0 3 0 0 0 2 44 2 0.0 3 0 0 0 2 45 39 0.0 3 0 0 0 39 46 11 0.0 3 0 0 0 11 47 5 0.0 3 0 0 0 5 48 4 0.0 3 0 0 0 4 49 2 0.0 3 0 0 0 2 50 4 0.0 3 0 0 0 4 51 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 55 2 0.0 3 0 0 0 2 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 58 4 0.0 3 0 0 0 4 59 3 0.0 3 0 0 0 3 60 1 0.0 3 0 0 0 1 61 2 0.0 3 0 0 0 2 62 2 0.0 3 0 0 0 2 63 1 0.0 3 0 0 0 1 64 3 0.0 3 0 0 0 3 65 1 0.0 3 0 0 0 1 66 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 1 68 1 0.0 3 0 0 1 71 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 3 0.0 3 0 0 0 3 89 3 0.0 3 0 0 0 3 90 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 92 7 0.0 3 0 0 0 7 94 5 0.0 3 0 0 0 5 95 2 0.0 3 0 0 0 2 96 5 0.0 3 0 0 0 5 97 3 0.0 3 0 0 0 3 98 9 0.0 3 0 0 1 8 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 4 0.0 3 0 0 0 4 102 3 0.0 3 0 0 0 3 104 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 106 4 0.0 3 0 0 0 4 107 2 0.0 3 0 0 0 2 108 4 0.0 3 0 0 1 3 109 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 114 104 0.0 3 0 0 1 103 115 72 0.0 3 0 0 0 72 116 3 0.0 3 0 0 0 3 117 12 0.0 3 0 0 0 12 118 29 0.0 3 0 0 1 28 119 9 0.0 3 0 0 0 9 120 2 0.0 3 0 0 0 2 121 3 0.0 3 0 0 0 3 124 2 0.0 3 0 0 0 2 134 1 0.0 3 0 0 0 1 137 4 0.0 3 0 0 0 4 138 3 0.0 3 0 0 0 3 140 4 0.0 3 0 0 0 4 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 146 3 0.0 3 0 0 0 3 148 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 153 4 0.0 3 0 0 0 4 159 3 0.0 3 0 0 0 3 162 1 0.0 3 0 0 0 1 163 4 0.0 3 0 0 0 4 164 3 0.0 3 0 0 0 3 165 8 0.0 3 0 0 0 8 166 5 0.0 3 0 0 0 5 167 2 0.0 3 0 0 0 2 168 1 0.0 3 0 0 0 1 169 2 0.0 3 0 0 1 1 170 1 0.0 3 0 0 0 1 171 3 0.0 3 0 0 0 3 172 3 0.0 3 0 0 0 3 173 1 0.0 3 0 0 0 1 174 7 0.0 3 0 0 0 7 175 3 0.0 3 0 0 0 3 176 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 178 3 0.0 3 0 0 0 3 179 3 0.0 3 0 0 0 3 181 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 215 2 0.0 3 0 0 0 2 216 2 0.0 3 0 0 0 2 217 150 0.0 3 0 0 8 142 218 4 0.0 3 0 0 0 4 221 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 238 2 0.0 3 0 0 0 2 244 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 279 7 0.0 3 0 0 0 7 280 2 0.0 3 0 0 0 2 281 4 0.0 3 0 0 0 4 282 2 0.0 3 0 0 0 2 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.66 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 45,835 Reads with adapters: 770 (1.7%) Reads written (passing filters): 45,835 (100.0%) Total basepairs processed: 12,626,922 bp Total written (filtered): 12,624,591 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 770 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 11.3% C: 74.4% G: 9.0% T: 5.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 756 716.2 0 756 4 8 179.0 0 8 5 5 44.8 0 5 6 1 11.2 0 1