This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAACAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 172.00 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 115,943 (3.2%) Reads that were too short: 51,530 (1.4%) Reads written (passing filters): 64,413 (1.8%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 16,151,978 bp (1.5%) === Adapter 1 === Sequence: TAACAT; Type: regular 5'; Length: 6; Trimmed: 115943 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 597 887.3 0 597 7 18978 887.3 0 18978 8 381 887.3 0 381 9 22460 887.3 0 22460 10 56 887.3 0 56 11 18 887.3 0 18 12 45 887.3 0 45 13 18 887.3 0 18 14 22 887.3 0 22 15 22 887.3 0 22 16 22 887.3 0 22 17 14 887.3 0 14 18 24 887.3 0 24 19 14 887.3 0 14 20 17 887.3 0 17 21 21 887.3 0 21 22 18 887.3 0 18 23 11 887.3 0 11 24 8 887.3 0 8 25 24 887.3 0 24 26 15 887.3 0 15 27 15 887.3 0 15 28 12 887.3 0 12 29 19 887.3 0 19 30 17 887.3 0 17 31 19 887.3 0 19 32 19 887.3 0 19 33 16 887.3 0 16 34 21 887.3 0 21 35 31 887.3 0 31 36 19 887.3 0 19 37 18 887.3 0 18 38 23 887.3 0 23 39 25 887.3 0 25 40 9 887.3 0 9 41 24 887.3 0 24 42 18 887.3 0 18 43 16 887.3 0 16 44 10 887.3 0 10 45 11 887.3 0 11 46 11 887.3 0 11 47 17 887.3 0 17 48 19 887.3 0 19 49 16 887.3 0 16 50 14 887.3 0 14 51 12 887.3 0 12 52 18 887.3 0 18 53 17 887.3 0 17 54 16 887.3 0 16 55 1540 887.3 0 1540 56 46 887.3 0 46 57 13 887.3 0 13 58 16 887.3 0 16 59 11 887.3 0 11 60 11 887.3 0 11 61 15 887.3 0 15 62 11 887.3 0 11 63 17 887.3 0 17 64 22 887.3 0 22 65 24 887.3 0 24 66 24 887.3 0 24 67 28 887.3 0 28 68 18 887.3 0 18 69 21 887.3 0 21 70 41 887.3 0 41 71 74 887.3 0 74 72 86 887.3 0 86 73 40 887.3 0 40 74 17 887.3 0 17 75 25 887.3 0 25 76 42 887.3 0 42 77 90 887.3 0 90 78 72 887.3 0 72 79 54 887.3 0 54 80 73 887.3 0 73 81 149 887.3 0 149 82 221 887.3 0 221 83 201 887.3 0 201 84 53 887.3 0 53 85 30 887.3 0 30 86 35 887.3 0 35 87 47 887.3 0 47 88 31 887.3 0 31 89 24 887.3 0 24 90 23 887.3 0 23 91 46 887.3 0 46 92 46 887.3 0 46 93 29 887.3 0 29 94 40 887.3 0 40 95 37 887.3 0 37 96 30 887.3 0 30 97 186 887.3 0 186 98 60 887.3 0 60 99 34 887.3 0 34 100 61 887.3 0 61 101 96 887.3 0 96 102 208 887.3 0 208 103 344 887.3 0 344 104 197 887.3 0 197 105 75 887.3 0 75 106 91 887.3 0 91 107 110 887.3 0 110 108 109 887.3 0 109 109 108 887.3 0 108 110 132 887.3 0 132 111 202 887.3 0 202 112 182 887.3 0 182 113 121 887.3 0 121 114 138 887.3 0 138 115 115 887.3 0 115 116 99 887.3 0 99 117 291 887.3 0 291 118 179 887.3 0 179 119 211 887.3 0 211 120 121 887.3 0 121 121 77 887.3 0 77 122 104 887.3 0 104 123 124 887.3 0 124 124 132 887.3 0 132 125 109 887.3 0 109 126 146 887.3 0 146 127 170 887.3 0 170 128 95 887.3 0 95 129 115 887.3 0 115 130 104 887.3 0 104 131 265 887.3 0 265 132 407 887.3 0 407 133 474 887.3 0 474 134 314 887.3 0 314 135 281 887.3 0 281 136 210 887.3 0 210 137 212 887.3 0 212 138 278 887.3 0 278 139 263 887.3 0 263 140 255 887.3 0 255 141 126 887.3 0 126 142 80 887.3 0 80 143 91 887.3 0 91 144 122 887.3 0 122 145 130 887.3 0 130 146 342 887.3 0 342 147 343 887.3 0 343 148 289 887.3 0 289 149 114 887.3 0 114 150 100 887.3 0 100 151 123 887.3 0 123 152 110 887.3 0 110 153 164 887.3 0 164 154 141 887.3 0 141 155 158 887.3 0 158 156 259 887.3 0 259 157 377 887.3 0 377 158 462 887.3 0 462 159 244 887.3 0 244 160 156 887.3 0 156 161 146 887.3 0 146 162 117 887.3 0 117 163 110 887.3 0 110 164 116 887.3 0 116 165 143 887.3 0 143 166 143 887.3 0 143 167 138 887.3 0 138 168 409 887.3 0 409 169 83 887.3 0 83 170 102 887.3 0 102 171 106 887.3 0 106 172 120 887.3 0 120 173 99 887.3 0 99 174 106 887.3 0 106 175 83 887.3 0 83 176 78 887.3 0 78 177 100 887.3 0 100 178 104 887.3 0 104 179 112 887.3 0 112 180 336 887.3 0 336 181 569 887.3 0 569 182 450 887.3 0 450 183 117 887.3 0 117 184 141 887.3 0 141 185 155 887.3 0 155 186 126 887.3 0 126 187 114 887.3 0 114 188 117 887.3 0 117 189 105 887.3 0 105 190 110 887.3 0 110 191 109 887.3 0 109 192 142 887.3 0 142 193 119 887.3 0 119 194 153 887.3 0 153 195 96 887.3 0 96 196 104 887.3 0 104 197 92 887.3 0 92 198 161 887.3 0 161 199 154 887.3 0 154 200 143 887.3 0 143 201 109 887.3 0 109 202 89 887.3 0 89 203 127 887.3 0 127 204 126 887.3 0 126 205 175 887.3 0 175 206 226 887.3 0 226 207 261 887.3 0 261 208 314 887.3 0 314 209 388 887.3 0 388 210 649 887.3 0 649 211 7397 887.3 0 7397 212 10128 887.3 0 10128 213 7795 887.3 0 7795 214 2821 887.3 0 2821 215 422 887.3 0 422 216 92 887.3 0 92 217 93 887.3 0 93 218 154 887.3 0 154 219 151 887.3 0 151 220 127 887.3 0 127 221 112 887.3 0 112 222 92 887.3 0 92 223 189 887.3 0 189 224 351 887.3 0 351 225 351 887.3 0 351 226 239 887.3 0 239 227 109 887.3 0 109 228 92 887.3 0 92 229 115 887.3 0 115 230 135 887.3 0 135 231 80 887.3 0 80 232 64 887.3 0 64 233 120 887.3 0 120 234 116 887.3 0 116 235 126 887.3 0 126 236 115 887.3 0 115 237 109 887.3 0 109 238 137 887.3 0 137 239 170 887.3 0 170 240 131 887.3 0 131 241 109 887.3 0 109 242 119 887.3 0 119 243 146 887.3 0 146 244 391 887.3 0 391 245 251 887.3 0 251 246 225 887.3 0 225 247 109 887.3 0 109 248 109 887.3 0 109 249 88 887.3 0 88 250 87 887.3 0 87 251 94 887.3 0 94 252 130 887.3 0 130 253 134 887.3 0 134 254 145 887.3 0 145 255 153 887.3 0 153 256 228 887.3 0 228 257 186 887.3 0 186 258 180 887.3 0 180 259 135 887.3 0 135 260 134 887.3 0 134 261 125 887.3 0 125 262 113 887.3 0 113 263 122 887.3 0 122 264 137 887.3 0 137 265 147 887.3 0 147 266 208 887.3 0 208 267 274 887.3 0 274 268 232 887.3 0 232 269 221 887.3 0 221 270 959 887.3 0 959 271 882 887.3 0 882 272 639 887.3 0 639 273 246 887.3 0 246 274 219 887.3 0 219 275 178 887.3 0 178 276 141 887.3 0 141 277 192 887.3 0 192 278 212 887.3 0 212 279 273 887.3 0 273 280 197 887.3 0 197 281 121 887.3 0 121 282 112 887.3 0 112 283 119 887.3 0 119 284 163 887.3 0 163 285 158 887.3 0 158 286 141 887.3 0 141 287 269 887.3 0 269 288 313 887.3 0 313 289 277 887.3 0 277 290 176 887.3 0 176 291 205 887.3 0 205 292 1694 887.3 0 1694 293 1814 887.3 0 1814 294 1080 887.3 0 1080 295 467 887.3 0 467 296 159 887.3 0 159 297 99 887.3 0 99 298 145 887.3 0 145 299 157 887.3 0 157 300 189 887.3 0 189 301 314 887.3 0 314 Finished in 4.61 s (72 us/read; 0.84 M reads/minute). === Summary === Total reads processed: 64,413 Reads with adapters: 23,178 (36.0%) Reads that were too short: 48 (0.1%) Reads written (passing filters): 23,130 (35.9%) Total basepairs processed: 16,151,978 bp Total written (filtered): 6,355,120 bp (39.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 23178 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 7 0.0 2 0 0 0 7 15 20 0.0 3 0 0 16 4 16 329 0.0 3 0 301 18 10 17 22562 0.0 3 21307 1059 167 29 18 49 0.0 3 2 41 3 3 19 83 0.0 3 23 12 23 25 20 29 0.0 3 1 0 0 28 21 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 26 1 0.0 3 0 1 29 1 0.0 3 1 30 1 0.0 3 0 0 0 1 33 7 0.0 3 0 0 0 7 35 2 0.0 3 0 0 0 2 36 3 0.0 3 0 0 1 2 40 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 52 2 0.0 3 0 1 0 1 53 2 0.0 3 0 0 0 2 55 9 0.0 3 0 0 0 9 56 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 1 1 58 1 0.0 3 0 0 0 1 59 3 0.0 3 0 0 0 3 60 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 2 0.0 3 1 0 0 1 67 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 69 2 0.0 3 0 0 0 2 72 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 90 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 112 2 0.0 3 0 0 0 2 113 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 146 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 162 3 0.0 3 0 0 0 3 167 1 0.0 3 0 0 1 190 1 0.0 3 0 0 1 194 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 228 2 0.0 3 0 0 0 2 229 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.35 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 23,130 Reads with adapters: 532 (2.3%) Reads written (passing filters): 23,130 (100.0%) Total basepairs processed: 6,355,120 bp Total written (filtered): 6,353,497 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 532 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 69.4% C: 9.4% G: 19.2% T: 2.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 506 361.4 0 506 4 25 90.4 0 25 5 1 22.6 0 1