This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAACAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 170.13 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 116,836 (3.2%) Reads that were too short: 51,466 (1.4%) Reads written (passing filters): 65,370 (1.8%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 16,409,521 bp (1.5%) === Adapter 1 === Sequence: TAACAT; Type: regular 5'; Length: 6; Trimmed: 116836 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 505 887.3 0 505 7 25341 887.3 0 25341 8 240 887.3 0 240 9 17783 887.3 0 17783 10 37 887.3 0 37 11 17 887.3 0 17 12 26 887.3 0 26 13 24 887.3 0 24 14 22 887.3 0 22 15 26 887.3 0 26 16 19 887.3 0 19 17 28 887.3 0 28 18 26 887.3 0 26 19 22 887.3 0 22 20 21 887.3 0 21 21 32 887.3 0 32 22 22 887.3 0 22 23 23 887.3 0 23 24 26 887.3 0 26 25 26 887.3 0 26 26 19 887.3 0 19 27 21 887.3 0 21 28 17 887.3 0 17 29 19 887.3 0 19 30 20 887.3 0 20 31 29 887.3 0 29 32 19 887.3 0 19 33 23 887.3 0 23 34 27 887.3 0 27 35 30 887.3 0 30 36 15 887.3 0 15 37 21 887.3 0 21 38 22 887.3 0 22 39 22 887.3 0 22 40 15 887.3 0 15 41 29 887.3 0 29 42 25 887.3 0 25 43 20 887.3 0 20 44 32 887.3 0 32 45 30 887.3 0 30 46 19 887.3 0 19 47 19 887.3 0 19 48 46 887.3 0 46 49 27 887.3 0 27 50 9 887.3 0 9 51 18 887.3 0 18 52 14 887.3 0 14 53 16 887.3 0 16 54 15 887.3 0 15 55 27 887.3 0 27 56 18 887.3 0 18 57 28 887.3 0 28 58 17 887.3 0 17 59 14 887.3 0 14 60 22 887.3 0 22 61 21 887.3 0 21 62 20 887.3 0 20 63 19 887.3 0 19 64 32 887.3 0 32 65 27 887.3 0 27 66 30 887.3 0 30 67 29 887.3 0 29 68 22 887.3 0 22 69 19 887.3 0 19 70 29 887.3 0 29 71 51 887.3 0 51 72 42 887.3 0 42 73 23 887.3 0 23 74 27 887.3 0 27 75 22 887.3 0 22 76 31 887.3 0 31 77 32 887.3 0 32 78 28 887.3 0 28 79 35 887.3 0 35 80 61 887.3 0 61 81 190 887.3 0 190 82 206 887.3 0 206 83 236 887.3 0 236 84 96 887.3 0 96 85 172 887.3 0 172 86 134 887.3 0 134 87 139 887.3 0 139 88 110 887.3 0 110 89 59 887.3 0 59 90 112 887.3 0 112 91 86 887.3 0 86 92 86 887.3 0 86 93 90 887.3 0 90 94 78 887.3 0 78 95 97 887.3 0 97 96 71 887.3 0 71 97 78 887.3 0 78 98 77 887.3 0 77 99 65 887.3 0 65 100 80 887.3 0 80 101 132 887.3 0 132 102 177 887.3 0 177 103 175 887.3 0 175 104 137 887.3 0 137 105 91 887.3 0 91 106 100 887.3 0 100 107 103 887.3 0 103 108 103 887.3 0 103 109 99 887.3 0 99 110 142 887.3 0 142 111 169 887.3 0 169 112 179 887.3 0 179 113 118 887.3 0 118 114 106 887.3 0 106 115 125 887.3 0 125 116 75 887.3 0 75 117 268 887.3 0 268 118 197 887.3 0 197 119 205 887.3 0 205 120 134 887.3 0 134 121 107 887.3 0 107 122 131 887.3 0 131 123 126 887.3 0 126 124 157 887.3 0 157 125 117 887.3 0 117 126 148 887.3 0 148 127 183 887.3 0 183 128 90 887.3 0 90 129 114 887.3 0 114 130 103 887.3 0 103 131 170 887.3 0 170 132 306 887.3 0 306 133 327 887.3 0 327 134 281 887.3 0 281 135 285 887.3 0 285 136 202 887.3 0 202 137 213 887.3 0 213 138 308 887.3 0 308 139 284 887.3 0 284 140 305 887.3 0 305 141 159 887.3 0 159 142 91 887.3 0 91 143 107 887.3 0 107 144 156 887.3 0 156 145 184 887.3 0 184 146 408 887.3 0 408 147 404 887.3 0 404 148 293 887.3 0 293 149 134 887.3 0 134 150 139 887.3 0 139 151 165 887.3 0 165 152 117 887.3 0 117 153 181 887.3 0 181 154 143 887.3 0 143 155 140 887.3 0 140 156 256 887.3 0 256 157 394 887.3 0 394 158 421 887.3 0 421 159 265 887.3 0 265 160 130 887.3 0 130 161 121 887.3 0 121 162 126 887.3 0 126 163 99 887.3 0 99 164 134 887.3 0 134 165 149 887.3 0 149 166 170 887.3 0 170 167 133 887.3 0 133 168 454 887.3 0 454 169 102 887.3 0 102 170 129 887.3 0 129 171 105 887.3 0 105 172 162 887.3 0 162 173 137 887.3 0 137 174 131 887.3 0 131 175 85 887.3 0 85 176 67 887.3 0 67 177 113 887.3 0 113 178 107 887.3 0 107 179 142 887.3 0 142 180 299 887.3 0 299 181 585 887.3 0 585 182 383 887.3 0 383 183 87 887.3 0 87 184 136 887.3 0 136 185 158 887.3 0 158 186 119 887.3 0 119 187 131 887.3 0 131 188 133 887.3 0 133 189 144 887.3 0 144 190 138 887.3 0 138 191 119 887.3 0 119 192 120 887.3 0 120 193 138 887.3 0 138 194 127 887.3 0 127 195 126 887.3 0 126 196 105 887.3 0 105 197 101 887.3 0 101 198 149 887.3 0 149 199 178 887.3 0 178 200 128 887.3 0 128 201 129 887.3 0 129 202 98 887.3 0 98 203 139 887.3 0 139 204 134 887.3 0 134 205 176 887.3 0 176 206 220 887.3 0 220 207 242 887.3 0 242 208 292 887.3 0 292 209 365 887.3 0 365 210 532 887.3 0 532 211 7740 887.3 0 7740 212 10885 887.3 0 10885 213 8197 887.3 0 8197 214 3112 887.3 0 3112 215 496 887.3 0 496 216 99 887.3 0 99 217 100 887.3 0 100 218 124 887.3 0 124 219 196 887.3 0 196 220 149 887.3 0 149 221 127 887.3 0 127 222 83 887.3 0 83 223 195 887.3 0 195 224 381 887.3 0 381 225 297 887.3 0 297 226 265 887.3 0 265 227 107 887.3 0 107 228 115 887.3 0 115 229 129 887.3 0 129 230 129 887.3 0 129 231 112 887.3 0 112 232 91 887.3 0 91 233 135 887.3 0 135 234 129 887.3 0 129 235 122 887.3 0 122 236 122 887.3 0 122 237 149 887.3 0 149 238 127 887.3 0 127 239 137 887.3 0 137 240 125 887.3 0 125 241 133 887.3 0 133 242 128 887.3 0 128 243 141 887.3 0 141 244 334 887.3 0 334 245 260 887.3 0 260 246 241 887.3 0 241 247 114 887.3 0 114 248 87 887.3 0 87 249 110 887.3 0 110 250 110 887.3 0 110 251 111 887.3 0 111 252 180 887.3 0 180 253 126 887.3 0 126 254 132 887.3 0 132 255 129 887.3 0 129 256 180 887.3 0 180 257 168 887.3 0 168 258 174 887.3 0 174 259 166 887.3 0 166 260 139 887.3 0 139 261 125 887.3 0 125 262 123 887.3 0 123 263 138 887.3 0 138 264 127 887.3 0 127 265 150 887.3 0 150 266 198 887.3 0 198 267 235 887.3 0 235 268 211 887.3 0 211 269 192 887.3 0 192 270 890 887.3 0 890 271 755 887.3 0 755 272 568 887.3 0 568 273 195 887.3 0 195 274 192 887.3 0 192 275 160 887.3 0 160 276 145 887.3 0 145 277 163 887.3 0 163 278 230 887.3 0 230 279 231 887.3 0 231 280 166 887.3 0 166 281 112 887.3 0 112 282 125 887.3 0 125 283 115 887.3 0 115 284 199 887.3 0 199 285 162 887.3 0 162 286 149 887.3 0 149 287 243 887.3 0 243 288 382 887.3 0 382 289 238 887.3 0 238 290 132 887.3 0 132 291 157 887.3 0 157 292 1230 887.3 0 1230 293 1363 887.3 0 1363 294 782 887.3 0 782 295 310 887.3 0 310 296 149 887.3 0 149 297 154 887.3 0 154 298 118 887.3 0 118 299 130 887.3 0 130 300 180 887.3 0 180 301 306 887.3 0 306 Finished in 4.91 s (75 us/read; 0.80 M reads/minute). === Summary === Total reads processed: 65,370 Reads with adapters: 25,749 (39.4%) Reads that were too short: 15 (0.0%) Reads written (passing filters): 25,734 (39.4%) Total basepairs processed: 16,409,521 bp Total written (filtered): 7,036,648 bp (42.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 25749 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 9 0.0 3 0 0 9 19 236 0.0 3 0 232 3 1 20 25284 0.0 4 24824 446 13 1 21 42 0.0 4 9 31 2 22 4 0.0 4 2 1 1 23 24 0.0 4 0 0 0 20 4 24 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 0 1 34 2 0.0 4 0 0 0 0 2 41 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 66 2 0.0 4 2 67 1 0.0 4 1 73 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 122 0.0 4 0 0 0 0 122 128 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 1 169 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.49 s (58 us/read; 1.04 M reads/minute). === Summary === Total reads processed: 25,734 Reads with adapters: 522 (2.0%) Reads written (passing filters): 25,734 (100.0%) Total basepairs processed: 7,036,648 bp Total written (filtered): 7,029,976 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 522 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 7.3% C: 4.4% G: 86.8% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 25 402.1 0 25 4 1 100.5 0 1 5 2 25.1 0 2 9 1 0.1 0 0 1 10 12 0.0 1 0 12 11 429 0.0 1 1 428 12 28 0.0 1 0 28 13 2 0.0 1 0 2 15 1 0.0 1 0 1 20 12 0.0 1 9 3 21 1 0.0 1 1 24 1 0.0 1 1 147 1 0.0 1 1 152 5 0.0 1 0 5 166 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.