This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACGTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 173.13 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 273,616 (7.5%) Reads that were too short: 152,560 (4.2%) Reads written (passing filters): 121,056 (3.3%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 29,479,558 bp (2.7%) === Adapter 1 === Sequence: CACGTA; Type: regular 5'; Length: 6; Trimmed: 273616 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1273 887.3 0 1273 7 1267 887.3 0 1267 8 38844 887.3 0 38844 9 32852 887.3 0 32852 10 207 887.3 0 207 11 342 887.3 0 342 12 83 887.3 0 83 13 226 887.3 0 226 14 126 887.3 0 126 15 70 887.3 0 70 16 67 887.3 0 67 17 96 887.3 0 96 18 68 887.3 0 68 19 41 887.3 0 41 20 72 887.3 0 72 21 94 887.3 0 94 22 45 887.3 0 45 23 35 887.3 0 35 24 70 887.3 0 70 25 61 887.3 0 61 26 64 887.3 0 64 27 55 887.3 0 55 28 60 887.3 0 60 29 60 887.3 0 60 30 54 887.3 0 54 31 78 887.3 0 78 32 55 887.3 0 55 33 40 887.3 0 40 34 49 887.3 0 49 35 48 887.3 0 48 36 43 887.3 0 43 37 57 887.3 0 57 38 96 887.3 0 96 39 57 887.3 0 57 40 69 887.3 0 69 41 64 887.3 0 64 42 96 887.3 0 96 43 112 887.3 0 112 44 90 887.3 0 90 45 68 887.3 0 68 46 141 887.3 0 141 47 126 887.3 0 126 48 113 887.3 0 113 49 75 887.3 0 75 50 74 887.3 0 74 51 60 887.3 0 60 52 82 887.3 0 82 53 76 887.3 0 76 54 76 887.3 0 76 55 59 887.3 0 59 56 93 887.3 0 93 57 73 887.3 0 73 58 114 887.3 0 114 59 70 887.3 0 70 60 84 887.3 0 84 61 93 887.3 0 93 62 77 887.3 0 77 63 48 887.3 0 48 64 59 887.3 0 59 65 52 887.3 0 52 66 78 887.3 0 78 67 69 887.3 0 69 68 137 887.3 0 137 69 110 887.3 0 110 70 85 887.3 0 85 71 59 887.3 0 59 72 99 887.3 0 99 73 141 887.3 0 141 74 122 887.3 0 122 75 148 887.3 0 148 76 63 887.3 0 63 77 94 887.3 0 94 78 119 887.3 0 119 79 67 887.3 0 67 80 52 887.3 0 52 81 62 887.3 0 62 82 69 887.3 0 69 83 83 887.3 0 83 84 97 887.3 0 97 85 81 887.3 0 81 86 67 887.3 0 67 87 90 887.3 0 90 88 62 887.3 0 62 89 68 887.3 0 68 90 71 887.3 0 71 91 61 887.3 0 61 92 69 887.3 0 69 93 115 887.3 0 115 94 84 887.3 0 84 95 107 887.3 0 107 96 76 887.3 0 76 97 200 887.3 0 200 98 205 887.3 0 205 99 127 887.3 0 127 100 74 887.3 0 74 101 69 887.3 0 69 102 69 887.3 0 69 103 79 887.3 0 79 104 66 887.3 0 66 105 80 887.3 0 80 106 106 887.3 0 106 107 107 887.3 0 107 108 102 887.3 0 102 109 80 887.3 0 80 110 80 887.3 0 80 111 73 887.3 0 73 112 112 887.3 0 112 113 104 887.3 0 104 114 81 887.3 0 81 115 138 887.3 0 138 116 149 887.3 0 149 117 159 887.3 0 159 118 136 887.3 0 136 119 182 887.3 0 182 120 285 887.3 0 285 121 170 887.3 0 170 122 216 887.3 0 216 123 193 887.3 0 193 124 371 887.3 0 371 125 8518 887.3 0 8518 126 2997 887.3 0 2997 127 1173 887.3 0 1173 128 149 887.3 0 149 129 124 887.3 0 124 130 143 887.3 0 143 131 116 887.3 0 116 132 82 887.3 0 82 133 91 887.3 0 91 134 185 887.3 0 185 135 267 887.3 0 267 136 267 887.3 0 267 137 146 887.3 0 146 138 168 887.3 0 168 139 117 887.3 0 117 140 113 887.3 0 113 141 116 887.3 0 116 142 131 887.3 0 131 143 169 887.3 0 169 144 103 887.3 0 103 145 367 887.3 0 367 146 474 887.3 0 474 147 338 887.3 0 338 148 169 887.3 0 169 149 136 887.3 0 136 150 130 887.3 0 130 151 123 887.3 0 123 152 138 887.3 0 138 153 114 887.3 0 114 154 186 887.3 0 186 155 185 887.3 0 185 156 168 887.3 0 168 157 248 887.3 0 248 158 247 887.3 0 247 159 266 887.3 0 266 160 138 887.3 0 138 161 145 887.3 0 145 162 152 887.3 0 152 163 148 887.3 0 148 164 158 887.3 0 158 165 243 887.3 0 243 166 246 887.3 0 246 167 221 887.3 0 221 168 144 887.3 0 144 169 115 887.3 0 115 170 118 887.3 0 118 171 236 887.3 0 236 172 222 887.3 0 222 173 1075 887.3 0 1075 174 1750 887.3 0 1750 175 1450 887.3 0 1450 176 573 887.3 0 573 177 136 887.3 0 136 178 162 887.3 0 162 179 203 887.3 0 203 180 235 887.3 0 235 181 1071 887.3 0 1071 182 715 887.3 0 715 183 940 887.3 0 940 184 349 887.3 0 349 185 297 887.3 0 297 186 1378 887.3 0 1378 187 1178 887.3 0 1178 188 1241 887.3 0 1241 189 227 887.3 0 227 190 163 887.3 0 163 191 178 887.3 0 178 192 149 887.3 0 149 193 198 887.3 0 198 194 184 887.3 0 184 195 159 887.3 0 159 196 140 887.3 0 140 197 104 887.3 0 104 198 90 887.3 0 90 199 178 887.3 0 178 200 165 887.3 0 165 201 201 887.3 0 201 202 204 887.3 0 204 203 196 887.3 0 196 204 176 887.3 0 176 205 190 887.3 0 190 206 483 887.3 0 483 207 682 887.3 0 682 208 566 887.3 0 566 209 333 887.3 0 333 210 124 887.3 0 124 211 164 887.3 0 164 212 343 887.3 0 343 213 211 887.3 0 211 214 320 887.3 0 320 215 378 887.3 0 378 216 219 887.3 0 219 217 240 887.3 0 240 218 323 887.3 0 323 219 398 887.3 0 398 220 500 887.3 0 500 221 486 887.3 0 486 222 541 887.3 0 541 223 570 887.3 0 570 224 1064 887.3 0 1064 225 16881 887.3 0 16881 226 15058 887.3 0 15058 227 9868 887.3 0 9868 228 435 887.3 0 435 229 1798 887.3 0 1798 230 5372 887.3 0 5372 231 5464 887.3 0 5464 232 2428 887.3 0 2428 233 363 887.3 0 363 234 269 887.3 0 269 235 257 887.3 0 257 236 188 887.3 0 188 237 197 887.3 0 197 238 184 887.3 0 184 239 173 887.3 0 173 240 195 887.3 0 195 241 251 887.3 0 251 242 240 887.3 0 240 243 235 887.3 0 235 244 250 887.3 0 250 245 300 887.3 0 300 246 384 887.3 0 384 247 348 887.3 0 348 248 383 887.3 0 383 249 289 887.3 0 289 250 744 887.3 0 744 251 848 887.3 0 848 252 1124 887.3 0 1124 253 1519 887.3 0 1519 254 1671 887.3 0 1671 255 661 887.3 0 661 256 167 887.3 0 167 257 173 887.3 0 173 258 166 887.3 0 166 259 236 887.3 0 236 260 200 887.3 0 200 261 211 887.3 0 211 262 242 887.3 0 242 263 273 887.3 0 273 264 303 887.3 0 303 265 319 887.3 0 319 266 277 887.3 0 277 267 300 887.3 0 300 268 295 887.3 0 295 269 262 887.3 0 262 270 221 887.3 0 221 271 520 887.3 0 520 272 390 887.3 0 390 273 486 887.3 0 486 274 399 887.3 0 399 275 753 887.3 0 753 276 404 887.3 0 404 277 353 887.3 0 353 278 567 887.3 0 567 279 1262 887.3 0 1262 280 4616 887.3 0 4616 281 5548 887.3 0 5548 282 3296 887.3 0 3296 283 743 887.3 0 743 284 609 887.3 0 609 285 849 887.3 0 849 286 362 887.3 0 362 287 312 887.3 0 312 288 281 887.3 0 281 289 462 887.3 0 462 290 552 887.3 0 552 291 393 887.3 0 393 292 304 887.3 0 304 293 254 887.3 0 254 294 530 887.3 0 530 295 908 887.3 0 908 296 1212 887.3 0 1212 297 1646 887.3 0 1646 298 2234 887.3 0 2234 299 3138 887.3 0 3138 300 5660 887.3 0 5660 301 32784 887.3 0 32784 Finished in 8.37 s (69 us/read; 0.87 M reads/minute). === Summary === Total reads processed: 121,056 Reads with adapters: 40,416 (33.4%) Reads that were too short: 175 (0.1%) Reads written (passing filters): 40,241 (33.2%) Total basepairs processed: 29,479,558 bp Total written (filtered): 11,055,172 bp (37.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 40416 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 12 0.0 2 0 0 0 12 15 18 0.0 3 0 0 15 3 16 353 0.0 3 0 316 24 13 17 39012 0.0 3 37142 1575 253 42 18 109 0.0 3 21 74 9 5 19 97 0.0 3 3 2 55 37 20 316 0.0 3 242 7 2 65 21 3 0.0 3 1 0 0 2 22 1 0.0 3 0 0 0 1 24 1 0.0 3 1 25 2 0.0 3 0 0 0 2 29 1 0.0 3 0 0 0 1 32 3 0.0 3 0 0 0 3 33 1 0.0 3 0 0 0 1 36 7 0.0 3 0 1 0 6 37 2 0.0 3 0 0 0 2 38 2 0.0 3 0 0 0 2 39 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 43 3 0.0 3 0 0 0 3 45 1 0.0 3 0 0 0 1 46 32 0.0 3 0 0 0 32 47 1 0.0 3 0 0 0 1 48 2 0.0 3 0 0 0 2 49 7 0.0 3 0 0 0 7 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 54 2 0.0 3 0 0 0 2 55 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 63 3 0.0 3 0 0 0 3 64 1 0.0 3 0 0 0 1 65 19 0.0 3 0 0 0 19 66 196 0.0 3 0 0 0 196 67 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 69 18 0.0 3 0 0 1 17 70 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 4 0.0 3 0 0 0 4 90 1 0.0 3 0 0 0 1 92 2 0.0 3 0 0 0 2 93 2 0.0 3 0 0 1 1 95 1 0.0 3 0 0 0 1 96 3 0.0 3 0 0 0 3 97 10 0.0 3 0 0 2 8 98 3 0.0 3 0 0 0 3 100 5 0.0 3 0 0 0 5 103 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 106 6 0.0 3 0 0 0 6 108 2 0.0 3 0 0 0 2 109 2 0.0 3 0 0 0 2 110 1 0.0 3 0 0 0 1 113 4 0.0 3 0 0 0 4 114 2 0.0 3 0 0 0 2 115 4 0.0 3 0 0 0 4 117 4 0.0 3 0 0 0 4 118 1 0.0 3 0 0 0 1 123 2 0.0 3 0 0 0 2 124 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 126 4 0.0 3 0 0 0 4 127 2 0.0 3 0 0 0 2 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 146 2 0.0 3 0 0 0 2 147 1 0.0 3 0 0 0 1 148 2 0.0 3 0 0 0 2 149 1 0.0 3 0 0 0 1 157 2 0.0 3 0 0 0 2 158 8 0.0 3 0 0 1 7 159 1 0.0 3 0 0 0 1 160 9 0.0 3 0 0 0 9 161 2 0.0 3 0 0 0 2 162 2 0.0 3 0 0 0 2 164 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 167 9 0.0 3 0 0 0 9 168 2 0.0 3 0 0 0 2 170 1 0.0 3 0 0 0 1 176 2 0.0 3 0 0 0 2 179 2 0.0 3 0 0 0 2 181 2 0.0 3 0 0 0 2 182 3 0.0 3 0 0 0 3 184 1 0.0 3 0 0 0 1 185 4 0.0 3 0 0 0 4 187 5 0.0 3 0 0 0 5 188 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 194 7 0.0 3 0 0 0 7 204 1 0.0 3 0 1 205 2 0.0 3 0 1 0 1 210 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 218 2 0.0 3 0 0 1 1 227 2 0.0 3 0 0 0 2 229 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 267 1 0.0 3 0 0 0 1 270 2 0.0 3 0 0 0 2 279 4 0.0 3 0 0 0 4 280 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.32 s (58 us/read; 1.04 M reads/minute). === Summary === Total reads processed: 40,241 Reads with adapters: 483 (1.2%) Reads written (passing filters): 40,241 (100.0%) Total basepairs processed: 11,055,172 bp Total written (filtered): 11,053,551 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 483 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 42.0% C: 9.1% G: 44.7% T: 4.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 320 628.8 0 320 4 154 157.2 0 154 5 9 39.3 0 9