This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACGTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 174.53 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 283,154 (7.8%) Reads that were too short: 165,681 (4.6%) Reads written (passing filters): 117,473 (3.2%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 29,537,782 bp (2.7%) === Adapter 1 === Sequence: CACGTA; Type: regular 5'; Length: 6; Trimmed: 283154 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 988 887.3 0 988 7 1025 887.3 0 1025 8 34023 887.3 0 34023 9 40097 887.3 0 40097 10 184 887.3 0 184 11 133 887.3 0 133 12 80 887.3 0 80 13 132 887.3 0 132 14 153 887.3 0 153 15 77 887.3 0 77 16 78 887.3 0 78 17 144 887.3 0 144 18 764 887.3 0 764 19 73 887.3 0 73 20 80 887.3 0 80 21 103 887.3 0 103 22 54 887.3 0 54 23 69 887.3 0 69 24 102 887.3 0 102 25 52 887.3 0 52 26 73 887.3 0 73 27 69 887.3 0 69 28 48 887.3 0 48 29 56 887.3 0 56 30 82 887.3 0 82 31 75 887.3 0 75 32 60 887.3 0 60 33 64 887.3 0 64 34 76 887.3 0 76 35 59 887.3 0 59 36 64 887.3 0 64 37 86 887.3 0 86 38 160 887.3 0 160 39 70 887.3 0 70 40 64 887.3 0 64 41 83 887.3 0 83 42 104 887.3 0 104 43 114 887.3 0 114 44 82 887.3 0 82 45 91 887.3 0 91 46 160 887.3 0 160 47 136 887.3 0 136 48 1167 887.3 0 1167 49 103 887.3 0 103 50 81 887.3 0 81 51 58 887.3 0 58 52 95 887.3 0 95 53 70 887.3 0 70 54 54 887.3 0 54 55 44 887.3 0 44 56 66 887.3 0 66 57 103 887.3 0 103 58 108 887.3 0 108 59 62 887.3 0 62 60 76 887.3 0 76 61 63 887.3 0 63 62 77 887.3 0 77 63 61 887.3 0 61 64 57 887.3 0 57 65 67 887.3 0 67 66 78 887.3 0 78 67 60 887.3 0 60 68 106 887.3 0 106 69 100 887.3 0 100 70 68 887.3 0 68 71 87 887.3 0 87 72 97 887.3 0 97 73 132 887.3 0 132 74 75 887.3 0 75 75 159 887.3 0 159 76 75 887.3 0 75 77 66 887.3 0 66 78 90 887.3 0 90 79 78 887.3 0 78 80 52 887.3 0 52 81 57 887.3 0 57 82 94 887.3 0 94 83 69 887.3 0 69 84 76 887.3 0 76 85 100 887.3 0 100 86 84 887.3 0 84 87 62 887.3 0 62 88 105 887.3 0 105 89 83 887.3 0 83 90 62 887.3 0 62 91 102 887.3 0 102 92 79 887.3 0 79 93 62 887.3 0 62 94 107 887.3 0 107 95 98 887.3 0 98 96 86 887.3 0 86 97 190 887.3 0 190 98 169 887.3 0 169 99 114 887.3 0 114 100 91 887.3 0 91 101 72 887.3 0 72 102 79 887.3 0 79 103 76 887.3 0 76 104 87 887.3 0 87 105 77 887.3 0 77 106 136 887.3 0 136 107 116 887.3 0 116 108 90 887.3 0 90 109 84 887.3 0 84 110 86 887.3 0 86 111 95 887.3 0 95 112 123 887.3 0 123 113 97 887.3 0 97 114 81 887.3 0 81 115 105 887.3 0 105 116 161 887.3 0 161 117 185 887.3 0 185 118 160 887.3 0 160 119 176 887.3 0 176 120 259 887.3 0 259 121 156 887.3 0 156 122 160 887.3 0 160 123 210 887.3 0 210 124 327 887.3 0 327 125 7718 887.3 0 7718 126 2796 887.3 0 2796 127 1079 887.3 0 1079 128 94 887.3 0 94 129 92 887.3 0 92 130 95 887.3 0 95 131 111 887.3 0 111 132 99 887.3 0 99 133 86 887.3 0 86 134 130 887.3 0 130 135 175 887.3 0 175 136 198 887.3 0 198 137 130 887.3 0 130 138 119 887.3 0 119 139 113 887.3 0 113 140 79 887.3 0 79 141 116 887.3 0 116 142 88 887.3 0 88 143 156 887.3 0 156 144 92 887.3 0 92 145 259 887.3 0 259 146 335 887.3 0 335 147 255 887.3 0 255 148 149 887.3 0 149 149 106 887.3 0 106 150 114 887.3 0 114 151 100 887.3 0 100 152 108 887.3 0 108 153 124 887.3 0 124 154 150 887.3 0 150 155 168 887.3 0 168 156 126 887.3 0 126 157 177 887.3 0 177 158 214 887.3 0 214 159 207 887.3 0 207 160 111 887.3 0 111 161 121 887.3 0 121 162 123 887.3 0 123 163 145 887.3 0 145 164 146 887.3 0 146 165 167 887.3 0 167 166 170 887.3 0 170 167 176 887.3 0 176 168 108 887.3 0 108 169 104 887.3 0 104 170 148 887.3 0 148 171 239 887.3 0 239 172 214 887.3 0 214 173 1025 887.3 0 1025 174 1612 887.3 0 1612 175 1052 887.3 0 1052 176 392 887.3 0 392 177 106 887.3 0 106 178 171 887.3 0 171 179 143 887.3 0 143 180 210 887.3 0 210 181 793 887.3 0 793 182 543 887.3 0 543 183 746 887.3 0 746 184 284 887.3 0 284 185 242 887.3 0 242 186 429 887.3 0 429 187 357 887.3 0 357 188 385 887.3 0 385 189 169 887.3 0 169 190 137 887.3 0 137 191 155 887.3 0 155 192 129 887.3 0 129 193 168 887.3 0 168 194 161 887.3 0 161 195 112 887.3 0 112 196 106 887.3 0 106 197 80 887.3 0 80 198 81 887.3 0 81 199 127 887.3 0 127 200 135 887.3 0 135 201 183 887.3 0 183 202 153 887.3 0 153 203 147 887.3 0 147 204 124 887.3 0 124 205 110 887.3 0 110 206 257 887.3 0 257 207 446 887.3 0 446 208 310 887.3 0 310 209 174 887.3 0 174 210 98 887.3 0 98 211 143 887.3 0 143 212 312 887.3 0 312 213 190 887.3 0 190 214 303 887.3 0 303 215 404 887.3 0 404 216 171 887.3 0 171 217 190 887.3 0 190 218 287 887.3 0 287 219 318 887.3 0 318 220 396 887.3 0 396 221 328 887.3 0 328 222 427 887.3 0 427 223 384 887.3 0 384 224 875 887.3 0 875 225 16542 887.3 0 16542 226 16104 887.3 0 16104 227 10212 887.3 0 10212 228 308 887.3 0 308 229 1833 887.3 0 1833 230 3967 887.3 0 3967 231 4015 887.3 0 4015 232 1086 887.3 0 1086 233 248 887.3 0 248 234 181 887.3 0 181 235 196 887.3 0 196 236 160 887.3 0 160 237 150 887.3 0 150 238 134 887.3 0 134 239 132 887.3 0 132 240 134 887.3 0 134 241 158 887.3 0 158 242 143 887.3 0 143 243 196 887.3 0 196 244 178 887.3 0 178 245 257 887.3 0 257 246 351 887.3 0 351 247 250 887.3 0 250 248 377 887.3 0 377 249 208 887.3 0 208 250 542 887.3 0 542 251 616 887.3 0 616 252 757 887.3 0 757 253 844 887.3 0 844 254 920 887.3 0 920 255 359 887.3 0 359 256 141 887.3 0 141 257 134 887.3 0 134 258 165 887.3 0 165 259 172 887.3 0 172 260 157 887.3 0 157 261 138 887.3 0 138 262 148 887.3 0 148 263 194 887.3 0 194 264 262 887.3 0 262 265 415 887.3 0 415 266 292 887.3 0 292 267 283 887.3 0 283 268 226 887.3 0 226 269 236 887.3 0 236 270 222 887.3 0 222 271 399 887.3 0 399 272 351 887.3 0 351 273 410 887.3 0 410 274 460 887.3 0 460 275 1272 887.3 0 1272 276 390 887.3 0 390 277 205 887.3 0 205 278 264 887.3 0 264 279 672 887.3 0 672 280 2065 887.3 0 2065 281 2577 887.3 0 2577 282 1480 887.3 0 1480 283 437 887.3 0 437 284 839 887.3 0 839 285 1554 887.3 0 1554 286 423 887.3 0 423 287 436 887.3 0 436 288 329 887.3 0 329 289 560 887.3 0 560 290 842 887.3 0 842 291 429 887.3 0 429 292 445 887.3 0 445 293 295 887.3 0 295 294 661 887.3 0 661 295 1138 887.3 0 1138 296 1624 887.3 0 1624 297 2659 887.3 0 2659 298 3762 887.3 0 3762 299 5395 887.3 0 5395 300 8763 887.3 0 8763 301 53255 887.3 0 53255 Finished in 8.96 s (76 us/read; 0.79 M reads/minute). === Summary === Total reads processed: 117,473 Reads with adapters: 41,469 (35.3%) Reads that were too short: 33 (0.0%) Reads written (passing filters): 41,436 (35.3%) Total basepairs processed: 29,537,782 bp Total written (filtered): 11,224,115 bp (38.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 41469 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 31 0.0 3 0 0 30 1 19 424 0.0 3 0 416 7 0 1 20 40317 0.0 4 39646 654 16 1 21 153 0.0 4 22 121 10 22 19 0.0 4 8 0 11 23 32 0.0 4 1 0 1 28 2 24 1 0.0 4 0 0 0 0 1 25 2 0.0 4 1 0 0 0 1 31 1 0.0 4 1 41 1 0.0 4 0 1 43 1 0.0 4 1 49 4 0.0 4 0 0 0 0 4 67 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 1 87 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 103 1 0.0 4 1 121 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 8 0.0 4 0 0 0 0 8 127 432 0.0 4 0 0 0 0 432 130 8 0.0 4 0 0 0 0 8 132 2 0.0 4 0 0 0 0 2 158 1 0.0 4 0 1 179 1 0.0 4 1 183 1 0.0 4 1 188 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 1 224 1 0.0 4 0 0 0 0 1 228 6 0.0 4 0 0 0 0 6 229 2 0.0 4 0 0 0 0 2 240 2 0.0 4 0 0 0 0 2 262 2 0.0 4 0 0 0 1 1 263 1 0.0 4 0 0 0 0 1 264 2 0.0 4 0 0 0 0 2 283 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.50 s (60 us/read; 0.99 M reads/minute). === Summary === Total reads processed: 41,436 Reads with adapters: 5,384 (13.0%) Reads written (passing filters): 41,436 (100.0%) Total basepairs processed: 11,224,115 bp Total written (filtered): 11,172,333 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5384 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.7% C: 2.4% G: 95.9% T: 1.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 38 647.4 0 38 4 7 161.9 0 7 5 2 40.5 0 2 6 3 10.1 0 3 8 1 0.6 0 1 9 2901 0.2 0 67 2834 10 1989 0.0 1 69 1920 11 431 0.0 1 1 430 12 5 0.0 1 0 5 13 3 0.0 1 0 3 135 1 0.0 1 1 150 1 0.0 1 0 1 208 1 0.0 1 0 1 272 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.