This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGCAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 172.66 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 107,988 (3.0%) Reads that were too short: 28,324 (0.8%) Reads written (passing filters): 79,664 (2.2%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 20,974,302 bp (1.9%) === Adapter 1 === Sequence: TTGCAA; Type: regular 5'; Length: 6; Trimmed: 107988 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1097 887.3 0 1097 7 30636 887.3 0 30636 8 25268 887.3 0 25268 9 91 887.3 0 91 10 61 887.3 0 61 11 65 887.3 0 65 12 42 887.3 0 42 13 58 887.3 0 58 14 58 887.3 0 58 15 65 887.3 0 65 16 47 887.3 0 47 17 76 887.3 0 76 18 77 887.3 0 77 19 59 887.3 0 59 20 62 887.3 0 62 21 70 887.3 0 70 22 66 887.3 0 66 23 66 887.3 0 66 24 53 887.3 0 53 25 57 887.3 0 57 26 59 887.3 0 59 27 52 887.3 0 52 28 51 887.3 0 51 29 66 887.3 0 66 30 54 887.3 0 54 31 41 887.3 0 41 32 73 887.3 0 73 33 53 887.3 0 53 34 57 887.3 0 57 35 53 887.3 0 53 36 42 887.3 0 42 37 52 887.3 0 52 38 81 887.3 0 81 39 602 887.3 0 602 40 779 887.3 0 779 41 599 887.3 0 599 42 287 887.3 0 287 43 135 887.3 0 135 44 47 887.3 0 47 45 45 887.3 0 45 46 59 887.3 0 59 47 60 887.3 0 60 48 52 887.3 0 52 49 53 887.3 0 53 50 54 887.3 0 54 51 81 887.3 0 81 52 77 887.3 0 77 53 65 887.3 0 65 54 62 887.3 0 62 55 60 887.3 0 60 56 56 887.3 0 56 57 55 887.3 0 55 58 57 887.3 0 57 59 54 887.3 0 54 60 80 887.3 0 80 61 65 887.3 0 65 62 45 887.3 0 45 63 48 887.3 0 48 64 75 887.3 0 75 65 56 887.3 0 56 66 74 887.3 0 74 67 56 887.3 0 56 68 44 887.3 0 44 69 52 887.3 0 52 70 67 887.3 0 67 71 73 887.3 0 73 72 94 887.3 0 94 73 66 887.3 0 66 74 96 887.3 0 96 75 53 887.3 0 53 76 56 887.3 0 56 77 74 887.3 0 74 78 148 887.3 0 148 79 75 887.3 0 75 80 116 887.3 0 116 81 72 887.3 0 72 82 66 887.3 0 66 83 74 887.3 0 74 84 85 887.3 0 85 85 82 887.3 0 82 86 79 887.3 0 79 87 68 887.3 0 68 88 52 887.3 0 52 89 76 887.3 0 76 90 56 887.3 0 56 91 117 887.3 0 117 92 161 887.3 0 161 93 118 887.3 0 118 94 80 887.3 0 80 95 90 887.3 0 90 96 94 887.3 0 94 97 137 887.3 0 137 98 557 887.3 0 557 99 498 887.3 0 498 100 369 887.3 0 369 101 127 887.3 0 127 102 148 887.3 0 148 103 96 887.3 0 96 104 99 887.3 0 99 105 88 887.3 0 88 106 77 887.3 0 77 107 104 887.3 0 104 108 218 887.3 0 218 109 78 887.3 0 78 110 96 887.3 0 96 111 224 887.3 0 224 112 264 887.3 0 264 113 202 887.3 0 202 114 69 887.3 0 69 115 72 887.3 0 72 116 70 887.3 0 70 117 69 887.3 0 69 118 91 887.3 0 91 119 64 887.3 0 64 120 80 887.3 0 80 121 82 887.3 0 82 122 45 887.3 0 45 123 84 887.3 0 84 124 135 887.3 0 135 125 159 887.3 0 159 126 76 887.3 0 76 127 60 887.3 0 60 128 80 887.3 0 80 129 93 887.3 0 93 130 106 887.3 0 106 131 75 887.3 0 75 132 90 887.3 0 90 133 396 887.3 0 396 134 96 887.3 0 96 135 148 887.3 0 148 136 87 887.3 0 87 137 128 887.3 0 128 138 152 887.3 0 152 139 140 887.3 0 140 140 73 887.3 0 73 141 71 887.3 0 71 142 120 887.3 0 120 143 130 887.3 0 130 144 92 887.3 0 92 145 109 887.3 0 109 146 149 887.3 0 149 147 207 887.3 0 207 148 205 887.3 0 205 149 108 887.3 0 108 150 110 887.3 0 110 151 74 887.3 0 74 152 77 887.3 0 77 153 88 887.3 0 88 154 96 887.3 0 96 155 200 887.3 0 200 156 99 887.3 0 99 157 98 887.3 0 98 158 148 887.3 0 148 159 168 887.3 0 168 160 192 887.3 0 192 161 63 887.3 0 63 162 97 887.3 0 97 163 119 887.3 0 119 164 125 887.3 0 125 165 66 887.3 0 66 166 109 887.3 0 109 167 97 887.3 0 97 168 111 887.3 0 111 169 108 887.3 0 108 170 228 887.3 0 228 171 194 887.3 0 194 172 352 887.3 0 352 173 123 887.3 0 123 174 95 887.3 0 95 175 65 887.3 0 65 176 92 887.3 0 92 177 162 887.3 0 162 178 200 887.3 0 200 179 223 887.3 0 223 180 150 887.3 0 150 181 94 887.3 0 94 182 107 887.3 0 107 183 202 887.3 0 202 184 300 887.3 0 300 185 244 887.3 0 244 186 178 887.3 0 178 187 134 887.3 0 134 188 165 887.3 0 165 189 219 887.3 0 219 190 140 887.3 0 140 191 175 887.3 0 175 192 148 887.3 0 148 193 179 887.3 0 179 194 120 887.3 0 120 195 129 887.3 0 129 196 112 887.3 0 112 197 87 887.3 0 87 198 87 887.3 0 87 199 112 887.3 0 112 200 78 887.3 0 78 201 102 887.3 0 102 202 92 887.3 0 92 203 104 887.3 0 104 204 99 887.3 0 99 205 104 887.3 0 104 206 96 887.3 0 96 207 103 887.3 0 103 208 108 887.3 0 108 209 124 887.3 0 124 210 178 887.3 0 178 211 198 887.3 0 198 212 170 887.3 0 170 213 168 887.3 0 168 214 668 887.3 0 668 215 965 887.3 0 965 216 497 887.3 0 497 217 188 887.3 0 188 218 102 887.3 0 102 219 104 887.3 0 104 220 107 887.3 0 107 221 108 887.3 0 108 222 165 887.3 0 165 223 1041 887.3 0 1041 224 804 887.3 0 804 225 1136 887.3 0 1136 226 129 887.3 0 129 227 119 887.3 0 119 228 140 887.3 0 140 229 143 887.3 0 143 230 162 887.3 0 162 231 91 887.3 0 91 232 93 887.3 0 93 233 90 887.3 0 90 234 105 887.3 0 105 235 120 887.3 0 120 236 122 887.3 0 122 237 159 887.3 0 159 238 195 887.3 0 195 239 205 887.3 0 205 240 275 887.3 0 275 241 227 887.3 0 227 242 238 887.3 0 238 243 150 887.3 0 150 244 97 887.3 0 97 245 106 887.3 0 106 246 105 887.3 0 105 247 101 887.3 0 101 248 108 887.3 0 108 249 167 887.3 0 167 250 201 887.3 0 201 251 209 887.3 0 209 252 121 887.3 0 121 253 112 887.3 0 112 254 147 887.3 0 147 255 157 887.3 0 157 256 240 887.3 0 240 257 238 887.3 0 238 258 184 887.3 0 184 259 144 887.3 0 144 260 148 887.3 0 148 261 145 887.3 0 145 262 151 887.3 0 151 263 540 887.3 0 540 264 723 887.3 0 723 265 465 887.3 0 465 266 97 887.3 0 97 267 125 887.3 0 125 268 94 887.3 0 94 269 110 887.3 0 110 270 176 887.3 0 176 271 392 887.3 0 392 272 1604 887.3 0 1604 273 2210 887.3 0 2210 274 2037 887.3 0 2037 275 197 887.3 0 197 276 264 887.3 0 264 277 388 887.3 0 388 278 288 887.3 0 288 279 143 887.3 0 143 280 110 887.3 0 110 281 115 887.3 0 115 282 148 887.3 0 148 283 378 887.3 0 378 284 786 887.3 0 786 285 757 887.3 0 757 286 413 887.3 0 413 287 175 887.3 0 175 288 188 887.3 0 188 289 110 887.3 0 110 290 101 887.3 0 101 291 96 887.3 0 96 292 108 887.3 0 108 293 103 887.3 0 103 294 119 887.3 0 119 295 121 887.3 0 121 296 84 887.3 0 84 297 147 887.3 0 147 298 307 887.3 0 307 299 394 887.3 0 394 300 376 887.3 0 376 301 262 887.3 0 262 Finished in 5.82 s (73 us/read; 0.82 M reads/minute). === Summary === Total reads processed: 79,664 Reads with adapters: 30,990 (38.9%) Reads that were too short: 82 (0.1%) Reads written (passing filters): 30,908 (38.8%) Total basepairs processed: 20,974,302 bp Total written (filtered): 8,540,906 bp (40.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 30990 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 8 0.0 2 0 0 0 8 15 18 0.0 3 0 0 11 7 16 265 0.0 3 0 221 23 21 17 30269 0.0 3 28639 1302 267 61 18 93 0.0 3 16 66 5 6 19 78 0.0 3 1 0 48 29 20 34 0.0 3 2 0 0 32 21 1 0.0 3 1 23 1 0.0 3 0 0 0 1 24 2 0.0 3 0 0 0 2 27 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 31 1 0.0 3 1 38 1 0.0 3 0 0 0 1 40 2 0.0 3 0 0 0 2 43 2 0.0 3 0 0 0 2 44 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 56 2 0.0 3 0 0 0 2 59 2 0.0 3 0 0 0 2 60 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 1 1 64 2 0.0 3 0 0 0 2 65 2 0.0 3 0 0 0 2 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 73 11 0.0 3 0 0 0 11 74 2 0.0 3 0 0 0 2 75 4 0.0 3 0 0 0 4 76 4 0.0 3 0 0 0 4 77 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 80 2 0.0 3 0 0 0 2 81 7 0.0 3 0 0 0 7 83 3 0.0 3 0 0 0 3 84 1 0.0 3 0 0 1 85 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 92 5 0.0 3 0 0 0 5 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 2 0.0 3 0 0 0 2 99 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 111 2 0.0 3 0 0 0 2 115 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 31 0.0 3 0 0 0 31 133 1 0.0 3 0 0 0 1 134 2 0.0 3 0 0 0 2 135 13 0.0 3 0 0 0 13 136 3 0.0 3 0 0 0 3 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 146 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 153 4 0.0 3 0 0 0 4 154 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 158 2 0.0 3 0 0 0 2 159 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 174 2 0.0 3 0 0 0 2 175 1 0.0 3 0 0 0 1 176 2 0.0 3 0 0 0 2 178 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 194 3 0.0 3 0 0 0 3 198 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 205 6 0.0 3 0 0 0 6 217 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 1 244 1 0.0 3 0 0 1 249 1 0.0 3 0 0 0 1 252 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 262 2 0.0 3 0 0 0 2 279 5 0.0 3 0 0 0 5 280 1 0.0 3 0 0 0 1 281 7 0.0 3 0 0 0 7 282 4 0.0 3 0 0 0 4 285 2 0.0 3 0 0 0 2 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.78 s (58 us/read; 1.04 M reads/minute). === Summary === Total reads processed: 30,908 Reads with adapters: 432 (1.4%) Reads written (passing filters): 30,908 (100.0%) Total basepairs processed: 8,540,906 bp Total written (filtered): 8,539,600 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 432 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 20.6% C: 70.8% G: 5.8% T: 2.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 423 482.9 0 423 4 8 120.7 0 8 5 1 30.2 0 1