This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGCAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 173.84 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 107,735 (3.0%) Reads that were too short: 24,132 (0.7%) Reads written (passing filters): 83,603 (2.3%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 22,057,677 bp (2.0%) === Adapter 1 === Sequence: TTGCAA; Type: regular 5'; Length: 6; Trimmed: 107735 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 893 887.3 0 893 7 26233 887.3 0 26233 8 31501 887.3 0 31501 9 107 887.3 0 107 10 85 887.3 0 85 11 83 887.3 0 83 12 93 887.3 0 93 13 92 887.3 0 92 14 106 887.3 0 106 15 83 887.3 0 83 16 79 887.3 0 79 17 82 887.3 0 82 18 72 887.3 0 72 19 99 887.3 0 99 20 96 887.3 0 96 21 111 887.3 0 111 22 86 887.3 0 86 23 69 887.3 0 69 24 64 887.3 0 64 25 84 887.3 0 84 26 73 887.3 0 73 27 87 887.3 0 87 28 83 887.3 0 83 29 80 887.3 0 80 30 77 887.3 0 77 31 97 887.3 0 97 32 99 887.3 0 99 33 73 887.3 0 73 34 65 887.3 0 65 35 106 887.3 0 106 36 86 887.3 0 86 37 76 887.3 0 76 38 122 887.3 0 122 39 463 887.3 0 463 40 521 887.3 0 521 41 748 887.3 0 748 42 272 887.3 0 272 43 127 887.3 0 127 44 69 887.3 0 69 45 69 887.3 0 69 46 114 887.3 0 114 47 79 887.3 0 79 48 42 887.3 0 42 49 79 887.3 0 79 50 85 887.3 0 85 51 80 887.3 0 80 52 68 887.3 0 68 53 84 887.3 0 84 54 87 887.3 0 87 55 73 887.3 0 73 56 61 887.3 0 61 57 103 887.3 0 103 58 90 887.3 0 90 59 71 887.3 0 71 60 108 887.3 0 108 61 69 887.3 0 69 62 59 887.3 0 59 63 64 887.3 0 64 64 67 887.3 0 67 65 119 887.3 0 119 66 107 887.3 0 107 67 1327 887.3 0 1327 68 111 887.3 0 111 69 74 887.3 0 74 70 97 887.3 0 97 71 109 887.3 0 109 72 88 887.3 0 88 73 95 887.3 0 95 74 131 887.3 0 131 75 97 887.3 0 97 76 93 887.3 0 93 77 88 887.3 0 88 78 170 887.3 0 170 79 93 887.3 0 93 80 152 887.3 0 152 81 72 887.3 0 72 82 79 887.3 0 79 83 97 887.3 0 97 84 99 887.3 0 99 85 73 887.3 0 73 86 85 887.3 0 85 87 72 887.3 0 72 88 82 887.3 0 82 89 60 887.3 0 60 90 88 887.3 0 88 91 129 887.3 0 129 92 169 887.3 0 169 93 193 887.3 0 193 94 97 887.3 0 97 95 93 887.3 0 93 96 108 887.3 0 108 97 111 887.3 0 111 98 341 887.3 0 341 99 359 887.3 0 359 100 284 887.3 0 284 101 112 887.3 0 112 102 121 887.3 0 121 103 119 887.3 0 119 104 94 887.3 0 94 105 106 887.3 0 106 106 82 887.3 0 82 107 135 887.3 0 135 108 293 887.3 0 293 109 85 887.3 0 85 110 96 887.3 0 96 111 183 887.3 0 183 112 142 887.3 0 142 113 152 887.3 0 152 114 97 887.3 0 97 115 85 887.3 0 85 116 105 887.3 0 105 117 95 887.3 0 95 118 98 887.3 0 98 119 88 887.3 0 88 120 106 887.3 0 106 121 74 887.3 0 74 122 82 887.3 0 82 123 126 887.3 0 126 124 163 887.3 0 163 125 185 887.3 0 185 126 87 887.3 0 87 127 106 887.3 0 106 128 96 887.3 0 96 129 93 887.3 0 93 130 93 887.3 0 93 131 110 887.3 0 110 132 118 887.3 0 118 133 407 887.3 0 407 134 93 887.3 0 93 135 190 887.3 0 190 136 80 887.3 0 80 137 115 887.3 0 115 138 115 887.3 0 115 139 102 887.3 0 102 140 96 887.3 0 96 141 96 887.3 0 96 142 109 887.3 0 109 143 115 887.3 0 115 144 83 887.3 0 83 145 131 887.3 0 131 146 111 887.3 0 111 147 205 887.3 0 205 148 180 887.3 0 180 149 121 887.3 0 121 150 102 887.3 0 102 151 111 887.3 0 111 152 87 887.3 0 87 153 104 887.3 0 104 154 99 887.3 0 99 155 195 887.3 0 195 156 97 887.3 0 97 157 110 887.3 0 110 158 154 887.3 0 154 159 178 887.3 0 178 160 207 887.3 0 207 161 80 887.3 0 80 162 107 887.3 0 107 163 109 887.3 0 109 164 106 887.3 0 106 165 92 887.3 0 92 166 94 887.3 0 94 167 92 887.3 0 92 168 100 887.3 0 100 169 97 887.3 0 97 170 202 887.3 0 202 171 190 887.3 0 190 172 276 887.3 0 276 173 141 887.3 0 141 174 88 887.3 0 88 175 83 887.3 0 83 176 114 887.3 0 114 177 143 887.3 0 143 178 177 887.3 0 177 179 203 887.3 0 203 180 117 887.3 0 117 181 95 887.3 0 95 182 113 887.3 0 113 183 194 887.3 0 194 184 301 887.3 0 301 185 199 887.3 0 199 186 218 887.3 0 218 187 106 887.3 0 106 188 119 887.3 0 119 189 162 887.3 0 162 190 118 887.3 0 118 191 141 887.3 0 141 192 134 887.3 0 134 193 146 887.3 0 146 194 88 887.3 0 88 195 120 887.3 0 120 196 104 887.3 0 104 197 96 887.3 0 96 198 105 887.3 0 105 199 99 887.3 0 99 200 88 887.3 0 88 201 90 887.3 0 90 202 95 887.3 0 95 203 86 887.3 0 86 204 112 887.3 0 112 205 89 887.3 0 89 206 94 887.3 0 94 207 95 887.3 0 95 208 78 887.3 0 78 209 106 887.3 0 106 210 163 887.3 0 163 211 140 887.3 0 140 212 134 887.3 0 134 213 123 887.3 0 123 214 353 887.3 0 353 215 421 887.3 0 421 216 260 887.3 0 260 217 117 887.3 0 117 218 76 887.3 0 76 219 78 887.3 0 78 220 96 887.3 0 96 221 107 887.3 0 107 222 138 887.3 0 138 223 952 887.3 0 952 224 717 887.3 0 717 225 1179 887.3 0 1179 226 93 887.3 0 93 227 84 887.3 0 84 228 116 887.3 0 116 229 150 887.3 0 150 230 141 887.3 0 141 231 75 887.3 0 75 232 83 887.3 0 83 233 96 887.3 0 96 234 82 887.3 0 82 235 96 887.3 0 96 236 99 887.3 0 99 237 116 887.3 0 116 238 182 887.3 0 182 239 168 887.3 0 168 240 207 887.3 0 207 241 184 887.3 0 184 242 172 887.3 0 172 243 144 887.3 0 144 244 82 887.3 0 82 245 100 887.3 0 100 246 77 887.3 0 77 247 82 887.3 0 82 248 84 887.3 0 84 249 143 887.3 0 143 250 172 887.3 0 172 251 158 887.3 0 158 252 106 887.3 0 106 253 94 887.3 0 94 254 119 887.3 0 119 255 135 887.3 0 135 256 157 887.3 0 157 257 132 887.3 0 132 258 121 887.3 0 121 259 110 887.3 0 110 260 122 887.3 0 122 261 128 887.3 0 128 262 140 887.3 0 140 263 553 887.3 0 553 264 794 887.3 0 794 265 572 887.3 0 572 266 90 887.3 0 90 267 106 887.3 0 106 268 104 887.3 0 104 269 109 887.3 0 109 270 129 887.3 0 129 271 302 887.3 0 302 272 1749 887.3 0 1749 273 2447 887.3 0 2447 274 2341 887.3 0 2341 275 184 887.3 0 184 276 123 887.3 0 123 277 170 887.3 0 170 278 153 887.3 0 153 279 121 887.3 0 121 280 110 887.3 0 110 281 91 887.3 0 91 282 106 887.3 0 106 283 217 887.3 0 217 284 402 887.3 0 402 285 452 887.3 0 452 286 287 887.3 0 287 287 123 887.3 0 123 288 151 887.3 0 151 289 113 887.3 0 113 290 99 887.3 0 99 291 79 887.3 0 79 292 64 887.3 0 64 293 99 887.3 0 99 294 84 887.3 0 84 295 85 887.3 0 85 296 78 887.3 0 78 297 104 887.3 0 104 298 198 887.3 0 198 299 301 887.3 0 301 300 307 887.3 0 307 301 176 887.3 0 176 Finished in 6.53 s (78 us/read; 0.77 M reads/minute). === Summary === Total reads processed: 83,603 Reads with adapters: 33,117 (39.6%) Reads that were too short: 19 (0.0%) Reads written (passing filters): 33,098 (39.6%) Total basepairs processed: 22,057,677 bp Total written (filtered): 8,940,423 bp (40.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 33117 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 11 0.0 3 0 0 11 19 331 0.0 3 0 326 3 0 2 20 31693 0.0 4 31167 492 32 2 21 119 0.0 4 33 85 1 22 11 0.0 4 0 2 9 23 24 0.0 4 4 0 0 19 1 24 1 0.0 4 0 0 0 0 1 28 1 0.0 4 0 0 0 0 1 36 33 0.0 4 0 0 0 0 33 87 3 0.0 4 0 0 0 0 3 89 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 125 3 0.0 4 0 0 0 0 3 126 8 0.0 4 0 0 0 0 8 127 852 0.0 4 0 0 0 0 852 128 3 0.0 4 0 0 0 0 3 130 1 0.0 4 0 0 0 0 1 149 3 0.0 4 0 0 0 0 3 153 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 229 4 0.0 4 0 0 0 0 4 233 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 1 247 1 0.0 4 0 0 0 0 1 261 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 283 2 0.0 4 0 0 0 0 2 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.92 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 33,098 Reads with adapters: 2,205 (6.7%) Reads written (passing filters): 33,098 (100.0%) Total basepairs processed: 8,940,423 bp Total written (filtered): 8,916,425 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2205 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.4% C: 2.8% G: 94.7% T: 1.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 16 517.2 0 16 4 6 129.3 0 6 9 17 0.1 0 0 17 10 308 0.0 1 4 304 11 1850 0.0 1 6 1844 12 2 0.0 1 0 2 13 3 0.0 1 0 3 14 1 0.0 1 0 1 96 1 0.0 1 1 170 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.