This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGCAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 178.10 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 406,302 (11.2%) Reads that were too short: 30,994 (0.9%) Reads written (passing filters): 375,308 (10.3%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 85,523,008 bp (7.8%) === Adapter 1 === Sequence: AAGCAG; Type: regular 5'; Length: 6; Trimmed: 406302 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2665 887.3 0 2665 7 72433 887.3 0 72433 8 2613 887.3 0 2613 9 83654 887.3 0 83654 10 278 887.3 0 278 11 223 887.3 0 223 12 158 887.3 0 158 13 124 887.3 0 124 14 151 887.3 0 151 15 161 887.3 0 161 16 155 887.3 0 155 17 182 887.3 0 182 18 152 887.3 0 152 19 126 887.3 0 126 20 181 887.3 0 181 21 567 887.3 0 567 22 422 887.3 0 422 23 374 887.3 0 374 24 1370 887.3 0 1370 25 1754 887.3 0 1754 26 1122 887.3 0 1122 27 154 887.3 0 154 28 160 887.3 0 160 29 151 887.3 0 151 30 121 887.3 0 121 31 136 887.3 0 136 32 150 887.3 0 150 33 154 887.3 0 154 34 122 887.3 0 122 35 159 887.3 0 159 36 151 887.3 0 151 37 133 887.3 0 133 38 104 887.3 0 104 39 148 887.3 0 148 40 121 887.3 0 121 41 137 887.3 0 137 42 114 887.3 0 114 43 117 887.3 0 117 44 145 887.3 0 145 45 143 887.3 0 143 46 139 887.3 0 139 47 168 887.3 0 168 48 150 887.3 0 150 49 136 887.3 0 136 50 130 887.3 0 130 51 134 887.3 0 134 52 128 887.3 0 128 53 161 887.3 0 161 54 181 887.3 0 181 55 188 887.3 0 188 56 277 887.3 0 277 57 259 887.3 0 259 58 162 887.3 0 162 59 162 887.3 0 162 60 180 887.3 0 180 61 787 887.3 0 787 62 981 887.3 0 981 63 682 887.3 0 682 64 128 887.3 0 128 65 161 887.3 0 161 66 179 887.3 0 179 67 156 887.3 0 156 68 231 887.3 0 231 69 364 887.3 0 364 70 464 887.3 0 464 71 323 887.3 0 323 72 192 887.3 0 192 73 269 887.3 0 269 74 254 887.3 0 254 75 281 887.3 0 281 76 180 887.3 0 180 77 177 887.3 0 177 78 377 887.3 0 377 79 285 887.3 0 285 80 397 887.3 0 397 81 292 887.3 0 292 82 541 887.3 0 541 83 3399 887.3 0 3399 84 4791 887.3 0 4791 85 2046 887.3 0 2046 86 245 887.3 0 245 87 184 887.3 0 184 88 340 887.3 0 340 89 185 887.3 0 185 90 179 887.3 0 179 91 184 887.3 0 184 92 195 887.3 0 195 93 166 887.3 0 166 94 210 887.3 0 210 95 167 887.3 0 167 96 191 887.3 0 191 97 279 887.3 0 279 98 275 887.3 0 275 99 499 887.3 0 499 100 198 887.3 0 198 101 246 887.3 0 246 102 334 887.3 0 334 103 292 887.3 0 292 104 281 887.3 0 281 105 435 887.3 0 435 106 399 887.3 0 399 107 494 887.3 0 494 108 484 887.3 0 484 109 651 887.3 0 651 110 987 887.3 0 987 111 2344 887.3 0 2344 112 39544 887.3 0 39544 113 32960 887.3 0 32960 114 10376 887.3 0 10376 115 262 887.3 0 262 116 209 887.3 0 209 117 237 887.3 0 237 118 317 887.3 0 317 119 518 887.3 0 518 120 4385 887.3 0 4385 121 3401 887.3 0 3401 122 2418 887.3 0 2418 123 449 887.3 0 449 124 522 887.3 0 522 125 2195 887.3 0 2195 126 9985 887.3 0 9985 127 489 887.3 0 489 128 197 887.3 0 197 129 550 887.3 0 550 130 339 887.3 0 339 131 421 887.3 0 421 132 181 887.3 0 181 133 191 887.3 0 191 134 246 887.3 0 246 135 248 887.3 0 248 136 358 887.3 0 358 137 392 887.3 0 392 138 294 887.3 0 294 139 252 887.3 0 252 140 259 887.3 0 259 141 314 887.3 0 314 142 672 887.3 0 672 143 628 887.3 0 628 144 694 887.3 0 694 145 401 887.3 0 401 146 489 887.3 0 489 147 3665 887.3 0 3665 148 6933 887.3 0 6933 149 2942 887.3 0 2942 150 616 887.3 0 616 151 329 887.3 0 329 152 326 887.3 0 326 153 249 887.3 0 249 154 192 887.3 0 192 155 186 887.3 0 186 156 222 887.3 0 222 157 346 887.3 0 346 158 234 887.3 0 234 159 329 887.3 0 329 160 299 887.3 0 299 161 282 887.3 0 282 162 344 887.3 0 344 163 386 887.3 0 386 164 428 887.3 0 428 165 479 887.3 0 479 166 1346 887.3 0 1346 167 1590 887.3 0 1590 168 1767 887.3 0 1767 169 1036 887.3 0 1036 170 1316 887.3 0 1316 171 3342 887.3 0 3342 172 3603 887.3 0 3603 173 2583 887.3 0 2583 174 623 887.3 0 623 175 402 887.3 0 402 176 269 887.3 0 269 177 199 887.3 0 199 178 248 887.3 0 248 179 212 887.3 0 212 180 190 887.3 0 190 181 279 887.3 0 279 182 349 887.3 0 349 183 1434 887.3 0 1434 184 1949 887.3 0 1949 185 1578 887.3 0 1578 186 707 887.3 0 707 187 764 887.3 0 764 188 586 887.3 0 586 189 440 887.3 0 440 190 276 887.3 0 276 191 1823 887.3 0 1823 192 3051 887.3 0 3051 193 2952 887.3 0 2952 194 1350 887.3 0 1350 195 389 887.3 0 389 196 394 887.3 0 394 197 272 887.3 0 272 198 254 887.3 0 254 199 388 887.3 0 388 200 352 887.3 0 352 201 394 887.3 0 394 202 318 887.3 0 318 203 146 887.3 0 146 204 153 887.3 0 153 205 197 887.3 0 197 206 235 887.3 0 235 207 250 887.3 0 250 208 285 887.3 0 285 209 186 887.3 0 186 210 230 887.3 0 230 211 189 887.3 0 189 212 168 887.3 0 168 213 194 887.3 0 194 214 198 887.3 0 198 215 229 887.3 0 229 216 263 887.3 0 263 217 245 887.3 0 245 218 233 887.3 0 233 219 254 887.3 0 254 220 205 887.3 0 205 221 163 887.3 0 163 222 207 887.3 0 207 223 190 887.3 0 190 224 253 887.3 0 253 225 521 887.3 0 521 226 784 887.3 0 784 227 608 887.3 0 608 228 834 887.3 0 834 229 1077 887.3 0 1077 230 656 887.3 0 656 231 178 887.3 0 178 232 199 887.3 0 199 233 171 887.3 0 171 234 178 887.3 0 178 235 175 887.3 0 175 236 171 887.3 0 171 237 193 887.3 0 193 238 217 887.3 0 217 239 196 887.3 0 196 240 227 887.3 0 227 241 185 887.3 0 185 242 193 887.3 0 193 243 359 887.3 0 359 244 185 887.3 0 185 245 290 887.3 0 290 246 387 887.3 0 387 247 361 887.3 0 361 248 256 887.3 0 256 249 159 887.3 0 159 250 127 887.3 0 127 251 373 887.3 0 373 252 389 887.3 0 389 253 242 887.3 0 242 254 196 887.3 0 196 255 258 887.3 0 258 256 283 887.3 0 283 257 231 887.3 0 231 258 205 887.3 0 205 259 205 887.3 0 205 260 229 887.3 0 229 261 228 887.3 0 228 262 208 887.3 0 208 263 185 887.3 0 185 264 220 887.3 0 220 265 204 887.3 0 204 266 228 887.3 0 228 267 1137 887.3 0 1137 268 2265 887.3 0 2265 269 979 887.3 0 979 270 220 887.3 0 220 271 191 887.3 0 191 272 158 887.3 0 158 273 132 887.3 0 132 274 173 887.3 0 173 275 251 887.3 0 251 276 220 887.3 0 220 277 511 887.3 0 511 278 344 887.3 0 344 279 254 887.3 0 254 280 210 887.3 0 210 281 248 887.3 0 248 282 280 887.3 0 280 283 251 887.3 0 251 284 187 887.3 0 187 285 147 887.3 0 147 286 168 887.3 0 168 287 158 887.3 0 158 288 166 887.3 0 166 289 174 887.3 0 174 290 178 887.3 0 178 291 175 887.3 0 175 292 173 887.3 0 173 293 208 887.3 0 208 294 237 887.3 0 237 295 250 887.3 0 250 296 218 887.3 0 218 297 528 887.3 0 528 298 692 887.3 0 692 299 1349 887.3 0 1349 300 212 887.3 0 212 301 188 887.3 0 188 Finished in 24.49 s (65 us/read; 0.92 M reads/minute). === Summary === Total reads processed: 375,308 Reads with adapters: 89,206 (23.8%) Reads that were too short: 702 (0.2%) Reads written (passing filters): 88,504 (23.6%) Total basepairs processed: 85,523,008 bp Total written (filtered): 24,106,331 bp (28.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 89206 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 10 0.0 2 0 0 0 10 15 51 0.0 3 0 0 36 15 16 671 0.0 3 0 594 48 29 17 85642 0.0 3 81269 3642 581 150 18 337 0.0 3 59 247 21 10 19 157 0.0 3 0 0 95 62 20 64 0.0 3 5 0 1 58 21 18 0.0 3 0 0 0 18 22 1 0.0 3 0 0 0 1 23 3 0.0 3 0 0 0 3 24 442 0.0 3 0 0 3 439 25 3 0.0 3 1 0 0 2 26 46 0.0 3 0 0 2 44 27 68 0.0 3 0 0 2 66 28 4 0.0 3 1 0 0 3 29 2 0.0 3 0 0 0 2 30 10 0.0 3 0 0 1 9 31 6 0.0 3 1 0 0 5 32 1 0.0 3 1 33 5 0.0 3 1 0 1 3 35 1 0.0 3 0 0 0 1 36 2 0.0 3 0 0 0 2 39 3 0.0 3 0 0 0 3 40 4 0.0 3 0 0 1 3 41 4 0.0 3 0 0 0 4 42 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 44 2 0.0 3 0 0 0 2 45 6 0.0 3 0 0 1 5 47 4 0.0 3 0 0 0 4 48 14 0.0 3 0 0 0 14 49 3 0.0 3 0 0 0 3 50 18 0.0 3 0 0 1 17 51 227 0.0 3 0 0 3 224 52 6 0.0 3 0 0 0 6 53 6 0.0 3 0 0 0 6 54 29 0.0 3 0 0 1 28 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 59 19 0.0 3 0 0 1 18 60 9 0.0 3 0 0 0 9 61 7 0.0 3 0 0 0 7 62 16 0.0 3 0 0 1 15 63 9 0.0 3 0 0 0 9 64 2 0.0 3 0 0 0 2 66 45 0.0 3 0 0 1 44 67 11 0.0 3 0 0 1 10 68 34 0.0 3 0 0 0 34 69 10 0.0 3 0 0 0 10 70 7 0.0 3 0 0 0 7 71 10 0.0 3 0 0 0 10 72 4 0.0 3 0 0 0 4 74 2 0.0 3 0 0 0 2 75 17 0.0 3 0 0 0 17 76 4 0.0 3 0 0 0 4 77 8 0.0 3 0 0 1 7 78 1 0.0 3 0 0 0 1 79 61 0.0 3 0 0 0 61 80 4 0.0 3 0 0 0 4 81 5 0.0 3 0 0 0 5 82 14 0.0 3 0 0 1 13 83 8 0.0 3 0 0 0 8 84 7 0.0 3 0 0 0 7 85 4 0.0 3 0 0 0 4 86 3 0.0 3 0 0 0 3 87 2 0.0 3 0 0 0 2 88 3 0.0 3 0 0 0 3 89 1 0.0 3 0 0 0 1 91 4 0.0 3 0 0 0 4 93 3 0.0 3 0 0 0 3 94 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 96 3 0.0 3 0 0 0 3 97 3 0.0 3 0 0 0 3 98 12 0.0 3 0 0 0 12 99 3 0.0 3 0 0 0 3 100 3 0.0 3 0 0 0 3 101 19 0.0 3 0 0 1 18 102 13 0.0 3 0 0 2 11 103 5 0.0 3 0 0 0 5 104 2 0.0 3 0 0 0 2 105 23 0.0 3 0 0 1 22 106 2 0.0 3 0 0 0 2 107 9 0.0 3 0 0 0 9 108 49 0.0 3 0 0 0 49 109 77 0.0 3 0 0 0 77 110 28 0.0 3 0 0 0 28 111 12 0.0 3 0 0 0 12 112 27 0.0 3 0 0 2 25 113 12 0.0 3 0 0 0 12 114 6 0.0 3 0 0 1 5 115 4 0.0 3 0 0 0 4 116 2 0.0 3 0 0 0 2 117 12 0.0 3 0 0 1 11 119 3 0.0 3 0 0 0 3 120 6 0.0 3 0 0 0 6 121 2 0.0 3 0 0 0 2 122 6 0.0 3 0 0 0 6 123 2 0.0 3 0 0 0 2 124 3 0.0 3 0 0 0 3 125 10 0.0 3 0 0 1 9 126 9 0.0 3 0 0 0 9 127 6 0.0 3 0 0 0 6 128 2 0.0 3 0 0 0 2 129 4 0.0 3 0 0 0 4 130 50 0.0 3 0 0 2 48 131 3 0.0 3 0 0 0 3 132 4 0.0 3 0 0 0 4 133 2 0.0 3 0 0 0 2 134 6 0.0 3 0 0 0 6 135 4 0.0 3 0 0 0 4 136 20 0.0 3 0 0 1 19 137 2 0.0 3 0 0 0 2 138 3 0.0 3 0 0 0 3 139 12 0.0 3 0 0 2 10 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 143 4 0.0 3 0 0 0 4 144 9 0.0 3 0 0 0 9 145 7 0.0 3 0 0 0 7 146 4 0.0 3 0 0 0 4 147 35 0.0 3 0 0 0 35 148 4 0.0 3 0 0 0 4 149 2 0.0 3 0 0 0 2 150 23 0.0 3 0 0 0 23 151 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 153 4 0.0 3 0 0 0 4 154 13 0.0 3 0 0 1 12 155 2 0.0 3 0 0 0 2 156 4 0.0 3 0 0 0 4 158 1 0.0 3 0 0 0 1 159 9 0.0 3 0 0 0 9 160 2 0.0 3 0 0 0 2 161 3 0.0 3 0 0 0 3 162 33 0.0 3 0 0 0 33 163 5 0.0 3 0 0 1 4 164 6 0.0 3 0 0 0 6 165 5 0.0 3 0 0 2 3 166 6 0.0 3 0 0 0 6 167 26 0.0 3 0 0 4 22 168 38 0.0 3 0 0 0 38 169 6 0.0 3 0 0 0 6 170 12 0.0 3 0 0 0 12 171 11 0.0 3 0 0 0 11 172 6 0.0 3 0 0 1 5 173 3 0.0 3 0 0 0 3 174 3 0.0 3 0 0 0 3 175 1 0.0 3 0 0 0 1 180 12 0.0 3 0 0 1 11 181 82 0.0 3 0 0 10 72 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 3 0.0 3 0 0 0 3 185 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 191 2 0.0 3 0 0 0 2 192 3 0.0 3 0 0 0 3 193 2 0.0 3 0 0 0 2 194 2 0.0 3 0 0 0 2 196 1 0.0 3 0 0 0 1 198 2 0.0 3 0 0 0 2 199 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 204 2 0.0 3 0 0 0 2 205 1 0.0 3 0 0 0 1 208 3 0.0 3 0 0 0 3 209 2 0.0 3 0 0 0 2 210 8 0.0 3 0 0 2 6 211 3 0.0 3 0 0 0 3 214 1 0.0 3 0 0 0 1 216 2 0.0 3 0 0 0 2 217 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 220 4 0.0 3 0 0 0 4 221 2 0.0 3 0 0 0 2 223 2 0.0 3 0 0 0 2 224 1 0.0 3 0 0 0 1 225 2 0.0 3 0 0 0 2 226 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 230 2 0.0 3 0 0 0 2 231 1 0.0 3 0 0 0 1 233 2 0.0 3 0 0 0 2 235 1 0.0 3 0 0 0 1 236 2 0.0 3 0 0 0 2 237 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 255 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 259 2 0.0 3 0 0 0 2 261 2 0.0 3 0 0 0 2 262 2 0.0 3 0 0 0 2 263 1 0.0 3 0 0 1 266 1 0.0 3 0 0 0 1 267 2 0.0 3 0 0 0 2 268 3 0.0 3 0 0 0 3 269 5 0.0 3 0 0 1 4 270 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 6 0.0 3 0 0 0 6 280 7 0.0 3 0 0 0 7 281 7 0.0 3 0 0 0 7 282 3 0.0 3 0 0 0 3 283 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.13 s (58 us/read; 1.04 M reads/minute). === Summary === Total reads processed: 88,504 Reads with adapters: 1,865 (2.1%) Reads written (passing filters): 88,504 (100.0%) Total basepairs processed: 24,106,331 bp Total written (filtered): 24,100,680 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1865 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 59.5% C: 13.4% G: 24.8% T: 2.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1815 1382.9 0 1815 4 47 345.7 0 47 5 2 86.4 0 2 8 1 1.4 0 1