This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGCAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 176.65 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 371,892 (10.2%) Reads that were too short: 28,470 (0.8%) Reads written (passing filters): 343,422 (9.4%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 80,762,886 bp (7.4%) === Adapter 1 === Sequence: AAGCAG; Type: regular 5'; Length: 6; Trimmed: 371892 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1925 887.3 0 1925 7 90522 887.3 0 90522 8 1741 887.3 0 1741 9 71785 887.3 0 71785 10 243 887.3 0 243 11 216 887.3 0 216 12 177 887.3 0 177 13 182 887.3 0 182 14 191 887.3 0 191 15 190 887.3 0 190 16 170 887.3 0 170 17 204 887.3 0 204 18 184 887.3 0 184 19 153 887.3 0 153 20 203 887.3 0 203 21 258 887.3 0 258 22 287 887.3 0 287 23 236 887.3 0 236 24 338 887.3 0 338 25 657 887.3 0 657 26 643 887.3 0 643 27 174 887.3 0 174 28 187 887.3 0 187 29 176 887.3 0 176 30 161 887.3 0 161 31 169 887.3 0 169 32 187 887.3 0 187 33 198 887.3 0 198 34 141 887.3 0 141 35 173 887.3 0 173 36 172 887.3 0 172 37 170 887.3 0 170 38 159 887.3 0 159 39 190 887.3 0 190 40 167 887.3 0 167 41 186 887.3 0 186 42 146 887.3 0 146 43 175 887.3 0 175 44 196 887.3 0 196 45 212 887.3 0 212 46 167 887.3 0 167 47 177 887.3 0 177 48 164 887.3 0 164 49 194 887.3 0 194 50 174 887.3 0 174 51 207 887.3 0 207 52 162 887.3 0 162 53 286 887.3 0 286 54 212 887.3 0 212 55 200 887.3 0 200 56 258 887.3 0 258 57 274 887.3 0 274 58 189 887.3 0 189 59 169 887.3 0 169 60 186 887.3 0 186 61 525 887.3 0 525 62 592 887.3 0 592 63 527 887.3 0 527 64 148 887.3 0 148 65 154 887.3 0 154 66 183 887.3 0 183 67 173 887.3 0 173 68 258 887.3 0 258 69 296 887.3 0 296 70 305 887.3 0 305 71 235 887.3 0 235 72 178 887.3 0 178 73 148 887.3 0 148 74 248 887.3 0 248 75 169 887.3 0 169 76 170 887.3 0 170 77 185 887.3 0 185 78 376 887.3 0 376 79 261 887.3 0 261 80 313 887.3 0 313 81 227 887.3 0 227 82 323 887.3 0 323 83 1226 887.3 0 1226 84 1591 887.3 0 1591 85 766 887.3 0 766 86 277 887.3 0 277 87 235 887.3 0 235 88 322 887.3 0 322 89 239 887.3 0 239 90 197 887.3 0 197 91 158 887.3 0 158 92 198 887.3 0 198 93 225 887.3 0 225 94 193 887.3 0 193 95 201 887.3 0 201 96 234 887.3 0 234 97 222 887.3 0 222 98 301 887.3 0 301 99 577 887.3 0 577 100 188 887.3 0 188 101 316 887.3 0 316 102 356 887.3 0 356 103 252 887.3 0 252 104 266 887.3 0 266 105 430 887.3 0 430 106 434 887.3 0 434 107 460 887.3 0 460 108 441 887.3 0 441 109 632 887.3 0 632 110 889 887.3 0 889 111 1853 887.3 0 1853 112 39818 887.3 0 39818 113 33812 887.3 0 33812 114 10630 887.3 0 10630 115 250 887.3 0 250 116 181 887.3 0 181 117 238 887.3 0 238 118 292 887.3 0 292 119 317 887.3 0 317 120 2162 887.3 0 2162 121 2345 887.3 0 2345 122 1502 887.3 0 1502 123 324 887.3 0 324 124 436 887.3 0 436 125 2019 887.3 0 2019 126 10272 887.3 0 10272 127 468 887.3 0 468 128 226 887.3 0 226 129 548 887.3 0 548 130 366 887.3 0 366 131 459 887.3 0 459 132 228 887.3 0 228 133 199 887.3 0 199 134 239 887.3 0 239 135 182 887.3 0 182 136 249 887.3 0 249 137 328 887.3 0 328 138 234 887.3 0 234 139 256 887.3 0 256 140 244 887.3 0 244 141 272 887.3 0 272 142 474 887.3 0 474 143 425 887.3 0 425 144 422 887.3 0 422 145 260 887.3 0 260 146 313 887.3 0 313 147 1295 887.3 0 1295 148 1394 887.3 0 1394 149 1194 887.3 0 1194 150 372 887.3 0 372 151 219 887.3 0 219 152 247 887.3 0 247 153 204 887.3 0 204 154 205 887.3 0 205 155 212 887.3 0 212 156 194 887.3 0 194 157 296 887.3 0 296 158 253 887.3 0 253 159 253 887.3 0 253 160 228 887.3 0 228 161 263 887.3 0 263 162 297 887.3 0 297 163 269 887.3 0 269 164 240 887.3 0 240 165 329 887.3 0 329 166 771 887.3 0 771 167 851 887.3 0 851 168 1002 887.3 0 1002 169 606 887.3 0 606 170 733 887.3 0 733 171 1854 887.3 0 1854 172 2268 887.3 0 2268 173 1923 887.3 0 1923 174 553 887.3 0 553 175 328 887.3 0 328 176 260 887.3 0 260 177 210 887.3 0 210 178 251 887.3 0 251 179 215 887.3 0 215 180 246 887.3 0 246 181 259 887.3 0 259 182 327 887.3 0 327 183 1539 887.3 0 1539 184 2186 887.3 0 2186 185 1569 887.3 0 1569 186 511 887.3 0 511 187 474 887.3 0 474 188 404 887.3 0 404 189 315 887.3 0 315 190 251 887.3 0 251 191 1190 887.3 0 1190 192 1799 887.3 0 1799 193 1714 887.3 0 1714 194 906 887.3 0 906 195 269 887.3 0 269 196 308 887.3 0 308 197 269 887.3 0 269 198 274 887.3 0 274 199 462 887.3 0 462 200 453 887.3 0 453 201 382 887.3 0 382 202 359 887.3 0 359 203 202 887.3 0 202 204 192 887.3 0 192 205 219 887.3 0 219 206 215 887.3 0 215 207 238 887.3 0 238 208 251 887.3 0 251 209 222 887.3 0 222 210 233 887.3 0 233 211 194 887.3 0 194 212 169 887.3 0 169 213 186 887.3 0 186 214 199 887.3 0 199 215 282 887.3 0 282 216 293 887.3 0 293 217 270 887.3 0 270 218 284 887.3 0 284 219 207 887.3 0 207 220 197 887.3 0 197 221 181 887.3 0 181 222 202 887.3 0 202 223 176 887.3 0 176 224 208 887.3 0 208 225 356 887.3 0 356 226 577 887.3 0 577 227 428 887.3 0 428 228 794 887.3 0 794 229 1083 887.3 0 1083 230 669 887.3 0 669 231 163 887.3 0 163 232 221 887.3 0 221 233 174 887.3 0 174 234 177 887.3 0 177 235 221 887.3 0 221 236 177 887.3 0 177 237 213 887.3 0 213 238 226 887.3 0 226 239 215 887.3 0 215 240 199 887.3 0 199 241 203 887.3 0 203 242 213 887.3 0 213 243 344 887.3 0 344 244 177 887.3 0 177 245 286 887.3 0 286 246 227 887.3 0 227 247 231 887.3 0 231 248 216 887.3 0 216 249 177 887.3 0 177 250 143 887.3 0 143 251 312 887.3 0 312 252 313 887.3 0 313 253 226 887.3 0 226 254 182 887.3 0 182 255 205 887.3 0 205 256 228 887.3 0 228 257 203 887.3 0 203 258 189 887.3 0 189 259 188 887.3 0 188 260 196 887.3 0 196 261 210 887.3 0 210 262 198 887.3 0 198 263 189 887.3 0 189 264 177 887.3 0 177 265 204 887.3 0 204 266 229 887.3 0 229 267 1117 887.3 0 1117 268 2370 887.3 0 2370 269 992 887.3 0 992 270 189 887.3 0 189 271 177 887.3 0 177 272 178 887.3 0 178 273 149 887.3 0 149 274 193 887.3 0 193 275 278 887.3 0 278 276 233 887.3 0 233 277 333 887.3 0 333 278 222 887.3 0 222 279 231 887.3 0 231 280 197 887.3 0 197 281 210 887.3 0 210 282 229 887.3 0 229 283 236 887.3 0 236 284 222 887.3 0 222 285 152 887.3 0 152 286 175 887.3 0 175 287 162 887.3 0 162 288 159 887.3 0 159 289 182 887.3 0 182 290 204 887.3 0 204 291 175 887.3 0 175 292 185 887.3 0 185 293 205 887.3 0 205 294 224 887.3 0 224 295 208 887.3 0 208 296 205 887.3 0 205 297 324 887.3 0 324 298 419 887.3 0 419 299 631 887.3 0 631 300 177 887.3 0 177 301 189 887.3 0 189 Finished in 24.48 s (71 us/read; 0.84 M reads/minute). === Summary === Total reads processed: 343,422 Reads with adapters: 92,659 (27.0%) Reads that were too short: 70 (0.0%) Reads written (passing filters): 92,589 (27.0%) Total basepairs processed: 80,762,886 bp Total written (filtered): 25,216,983 bp (31.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 92659 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 1 2 18 35 0.0 3 0 0 33 2 19 767 0.0 3 0 738 21 5 3 20 90043 0.0 4 88554 1435 44 8 2 21 252 0.0 4 62 183 5 2 22 18 0.0 4 3 1 9 2 3 23 55 0.0 4 6 0 0 44 5 24 5 0.0 4 0 0 0 0 5 25 2 0.0 4 1 0 0 0 1 26 1 0.0 4 1 29 1 0.0 4 0 0 0 0 1 31 2 0.0 4 0 0 0 0 2 36 1 0.0 4 1 41 1 0.0 4 1 48 1 0.0 4 0 0 0 0 1 49 2 0.0 4 0 0 0 0 2 50 1 0.0 4 0 0 0 0 1 51 106 0.0 4 0 0 0 0 106 59 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 72 2 0.0 4 0 0 0 0 2 73 1 0.0 4 1 77 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 98 2 0.0 4 0 0 0 0 2 99 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 110 14 0.0 4 0 0 0 0 14 112 1 0.0 4 0 0 0 0 1 113 6 0.0 4 0 0 0 0 6 123 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 6 0.0 4 0 0 0 0 6 126 16 0.0 4 0 0 0 0 16 127 1239 0.0 4 0 0 0 1 1238 128 5 0.0 4 0 0 0 0 5 129 5 0.0 4 0 0 0 0 5 130 2 0.0 4 0 0 0 0 2 152 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 157 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 168 1 0.0 4 1 170 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 177 11 0.0 4 0 0 0 0 11 178 2 0.0 4 0 0 0 0 2 183 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 208 4 0.0 4 0 0 0 0 4 210 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 0 0 0 1 228 4 0.0 4 0 0 0 0 4 229 4 0.0 4 0 0 0 0 4 230 3 0.0 4 0 0 0 0 3 246 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 1 268 1 0.0 4 0 0 0 0 1 271 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.41 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 92,589 Reads with adapters: 1,871 (2.0%) Reads written (passing filters): 92,589 (100.0%) Total basepairs processed: 25,216,983 bp Total written (filtered): 25,196,907 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1871 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 6.0% C: 3.4% G: 88.3% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 116 1446.7 0 116 4 15 361.7 0 15 5 25 90.4 0 25 9 6 0.4 0 1 5 10 32 0.1 1 0 32 11 1595 0.0 1 6 1589 12 73 0.0 1 2 71 13 2 0.0 1 0 2 15 1 0.0 1 0 1 45 2 0.0 1 2 114 1 0.0 1 1 152 1 0.0 1 0 1 160 1 0.0 1 0 1 191 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.