This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGAGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 171.60 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 250,487 (6.9%) Reads that were too short: 58,468 (1.6%) Reads written (passing filters): 192,019 (5.3%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 53,094,427 bp (4.9%) === Adapter 1 === Sequence: TTGAGT; Type: regular 5'; Length: 6; Trimmed: 250487 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 808 887.3 0 808 7 2000 887.3 0 2000 8 93542 887.3 0 93542 9 65460 887.3 0 65460 10 171 887.3 0 171 11 109 887.3 0 109 12 145 887.3 0 145 13 99 887.3 0 99 14 130 887.3 0 130 15 116 887.3 0 116 16 123 887.3 0 123 17 107 887.3 0 107 18 130 887.3 0 130 19 152 887.3 0 152 20 110 887.3 0 110 21 119 887.3 0 119 22 127 887.3 0 127 23 126 887.3 0 126 24 104 887.3 0 104 25 123 887.3 0 123 26 106 887.3 0 106 27 109 887.3 0 109 28 112 887.3 0 112 29 137 887.3 0 137 30 121 887.3 0 121 31 117 887.3 0 117 32 122 887.3 0 122 33 116 887.3 0 116 34 132 887.3 0 132 35 138 887.3 0 138 36 128 887.3 0 128 37 116 887.3 0 116 38 116 887.3 0 116 39 95 887.3 0 95 40 116 887.3 0 116 41 122 887.3 0 122 42 132 887.3 0 132 43 114 887.3 0 114 44 167 887.3 0 167 45 152 887.3 0 152 46 113 887.3 0 113 47 111 887.3 0 111 48 138 887.3 0 138 49 122 887.3 0 122 50 117 887.3 0 117 51 120 887.3 0 120 52 136 887.3 0 136 53 149 887.3 0 149 54 136 887.3 0 136 55 89 887.3 0 89 56 122 887.3 0 122 57 120 887.3 0 120 58 138 887.3 0 138 59 250 887.3 0 250 60 330 887.3 0 330 61 335 887.3 0 335 62 125 887.3 0 125 63 105 887.3 0 105 64 116 887.3 0 116 65 104 887.3 0 104 66 116 887.3 0 116 67 142 887.3 0 142 68 128 887.3 0 128 69 128 887.3 0 128 70 172 887.3 0 172 71 165 887.3 0 165 72 187 887.3 0 187 73 148 887.3 0 148 74 112 887.3 0 112 75 135 887.3 0 135 76 196 887.3 0 196 77 277 887.3 0 277 78 302 887.3 0 302 79 145 887.3 0 145 80 156 887.3 0 156 81 155 887.3 0 155 82 149 887.3 0 149 83 151 887.3 0 151 84 164 887.3 0 164 85 200 887.3 0 200 86 132 887.3 0 132 87 148 887.3 0 148 88 144 887.3 0 144 89 139 887.3 0 139 90 159 887.3 0 159 91 161 887.3 0 161 92 186 887.3 0 186 93 134 887.3 0 134 94 144 887.3 0 144 95 161 887.3 0 161 96 114 887.3 0 114 97 135 887.3 0 135 98 161 887.3 0 161 99 126 887.3 0 126 100 146 887.3 0 146 101 129 887.3 0 129 102 137 887.3 0 137 103 106 887.3 0 106 104 126 887.3 0 126 105 144 887.3 0 144 106 161 887.3 0 161 107 140 887.3 0 140 108 117 887.3 0 117 109 136 887.3 0 136 110 240 887.3 0 240 111 261 887.3 0 261 112 238 887.3 0 238 113 143 887.3 0 143 114 174 887.3 0 174 115 116 887.3 0 116 116 143 887.3 0 143 117 181 887.3 0 181 118 618 887.3 0 618 119 160 887.3 0 160 120 120 887.3 0 120 121 132 887.3 0 132 122 123 887.3 0 123 123 124 887.3 0 124 124 132 887.3 0 132 125 160 887.3 0 160 126 158 887.3 0 158 127 138 887.3 0 138 128 147 887.3 0 147 129 168 887.3 0 168 130 192 887.3 0 192 131 218 887.3 0 218 132 236 887.3 0 236 133 247 887.3 0 247 134 232 887.3 0 232 135 166 887.3 0 166 136 163 887.3 0 163 137 172 887.3 0 172 138 227 887.3 0 227 139 295 887.3 0 295 140 229 887.3 0 229 141 183 887.3 0 183 142 148 887.3 0 148 143 196 887.3 0 196 144 329 887.3 0 329 145 174 887.3 0 174 146 164 887.3 0 164 147 176 887.3 0 176 148 185 887.3 0 185 149 157 887.3 0 157 150 145 887.3 0 145 151 139 887.3 0 139 152 200 887.3 0 200 153 195 887.3 0 195 154 137 887.3 0 137 155 168 887.3 0 168 156 174 887.3 0 174 157 166 887.3 0 166 158 174 887.3 0 174 159 145 887.3 0 145 160 127 887.3 0 127 161 138 887.3 0 138 162 132 887.3 0 132 163 152 887.3 0 152 164 139 887.3 0 139 165 162 887.3 0 162 166 135 887.3 0 135 167 148 887.3 0 148 168 155 887.3 0 155 169 141 887.3 0 141 170 121 887.3 0 121 171 167 887.3 0 167 172 116 887.3 0 116 173 118 887.3 0 118 174 122 887.3 0 122 175 136 887.3 0 136 176 134 887.3 0 134 177 139 887.3 0 139 178 110 887.3 0 110 179 137 887.3 0 137 180 134 887.3 0 134 181 142 887.3 0 142 182 127 887.3 0 127 183 154 887.3 0 154 184 136 887.3 0 136 185 137 887.3 0 137 186 124 887.3 0 124 187 150 887.3 0 150 188 132 887.3 0 132 189 116 887.3 0 116 190 149 887.3 0 149 191 127 887.3 0 127 192 210 887.3 0 210 193 207 887.3 0 207 194 133 887.3 0 133 195 130 887.3 0 130 196 180 887.3 0 180 197 311 887.3 0 311 198 506 887.3 0 506 199 330 887.3 0 330 200 147 887.3 0 147 201 141 887.3 0 141 202 124 887.3 0 124 203 100 887.3 0 100 204 243 887.3 0 243 205 273 887.3 0 273 206 189 887.3 0 189 207 119 887.3 0 119 208 103 887.3 0 103 209 134 887.3 0 134 210 127 887.3 0 127 211 127 887.3 0 127 212 132 887.3 0 132 213 128 887.3 0 128 214 129 887.3 0 129 215 108 887.3 0 108 216 119 887.3 0 119 217 157 887.3 0 157 218 149 887.3 0 149 219 121 887.3 0 121 220 115 887.3 0 115 221 148 887.3 0 148 222 159 887.3 0 159 223 304 887.3 0 304 224 344 887.3 0 344 225 184 887.3 0 184 226 155 887.3 0 155 227 131 887.3 0 131 228 141 887.3 0 141 229 136 887.3 0 136 230 157 887.3 0 157 231 148 887.3 0 148 232 153 887.3 0 153 233 206 887.3 0 206 234 226 887.3 0 226 235 169 887.3 0 169 236 164 887.3 0 164 237 197 887.3 0 197 238 212 887.3 0 212 239 201 887.3 0 201 240 278 887.3 0 278 241 214 887.3 0 214 242 273 887.3 0 273 243 233 887.3 0 233 244 244 887.3 0 244 245 305 887.3 0 305 246 297 887.3 0 297 247 283 887.3 0 283 248 136 887.3 0 136 249 150 887.3 0 150 250 123 887.3 0 123 251 135 887.3 0 135 252 164 887.3 0 164 253 171 887.3 0 171 254 179 887.3 0 179 255 222 887.3 0 222 256 223 887.3 0 223 257 245 887.3 0 245 258 235 887.3 0 235 259 226 887.3 0 226 260 209 887.3 0 209 261 242 887.3 0 242 262 206 887.3 0 206 263 218 887.3 0 218 264 257 887.3 0 257 265 946 887.3 0 946 266 1512 887.3 0 1512 267 931 887.3 0 931 268 565 887.3 0 565 269 122 887.3 0 122 270 147 887.3 0 147 271 233 887.3 0 233 272 280 887.3 0 280 273 247 887.3 0 247 274 302 887.3 0 302 275 240 887.3 0 240 276 157 887.3 0 157 277 196 887.3 0 196 278 229 887.3 0 229 279 232 887.3 0 232 280 220 887.3 0 220 281 252 887.3 0 252 282 227 887.3 0 227 283 202 887.3 0 202 284 266 887.3 0 266 285 238 887.3 0 238 286 295 887.3 0 295 287 218 887.3 0 218 288 267 887.3 0 267 289 262 887.3 0 262 290 303 887.3 0 303 291 403 887.3 0 403 292 424 887.3 0 424 293 460 887.3 0 460 294 461 887.3 0 461 295 449 887.3 0 449 296 607 887.3 0 607 297 1732 887.3 0 1732 298 8211 887.3 0 8211 299 10837 887.3 0 10837 300 9647 887.3 0 9647 301 4448 887.3 0 4448 Finished in 14.17 s (74 us/read; 0.81 M reads/minute). === Summary === Total reads processed: 192,019 Reads with adapters: 91,377 (47.6%) Reads that were too short: 96 (0.0%) Reads written (passing filters): 91,281 (47.5%) Total basepairs processed: 53,094,427 bp Total written (filtered): 25,188,728 bp (47.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 91377 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 11 0.0 2 0 0 0 11 15 53 0.0 3 0 0 34 19 16 743 0.0 3 0 657 56 30 17 89861 0.0 3 85350 3753 630 128 18 277 0.0 3 33 213 23 8 19 198 0.0 3 1 0 116 81 20 80 0.0 3 2 1 0 77 22 1 0.0 3 1 24 1 0.0 3 1 25 2 0.0 3 2 28 1 0.0 3 1 29 2 0.0 3 1 0 0 1 30 2 0.0 3 1 0 1 31 2 0.0 3 1 0 0 1 32 1 0.0 3 1 33 1 0.0 3 1 34 1 0.0 3 1 39 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 1 59 2 0.0 3 0 0 1 1 61 6 0.0 3 0 0 0 6 62 5 0.0 3 0 0 0 5 64 2 0.0 3 0 0 0 2 66 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 78 2 0.0 3 0 0 0 2 81 1 0.0 3 0 0 0 1 83 2 0.0 3 0 0 0 2 90 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 112 2 0.0 3 0 0 0 2 114 2 0.0 3 0 0 0 2 115 1 0.0 3 0 0 0 1 116 3 0.0 3 0 0 0 3 121 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 149 4 0.0 3 0 0 0 4 151 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 164 2 0.0 3 0 0 0 2 166 2 0.0 3 0 0 0 2 172 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 1 189 40 0.0 3 0 0 0 40 191 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 0 2 224 2 0.0 3 1 0 0 1 228 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 237 2 0.0 3 0 0 0 2 265 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 279 6 0.0 3 0 0 0 6 280 2 0.0 3 0 0 0 2 281 10 0.0 3 0 0 0 10 282 3 0.0 3 0 0 0 3 288 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.28 s (58 us/read; 1.04 M reads/minute). === Summary === Total reads processed: 91,281 Reads with adapters: 447 (0.5%) Reads written (passing filters): 91,281 (100.0%) Total basepairs processed: 25,188,728 bp Total written (filtered): 25,187,230 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 447 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 41.8% C: 16.3% G: 38.0% T: 3.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 331 1426.3 0 331 4 107 356.6 0 107 5 7 89.1 0 7 7 1 5.6 0 1 35 1 0.0 2 0 0 1