This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTCAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 178.88 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 207,281 (5.7%) Reads that were too short: 37,744 (1.0%) Reads written (passing filters): 169,537 (4.7%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 46,624,336 bp (4.3%) === Adapter 1 === Sequence: CGTCAC; Type: regular 5'; Length: 6; Trimmed: 207281 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3325 887.3 0 3325 7 75381 887.3 0 75381 8 60867 887.3 0 60867 9 654 887.3 0 654 10 140 887.3 0 140 11 96 887.3 0 96 12 75 887.3 0 75 13 93 887.3 0 93 14 92 887.3 0 92 15 77 887.3 0 77 16 87 887.3 0 87 17 75 887.3 0 75 18 88 887.3 0 88 19 110 887.3 0 110 20 78 887.3 0 78 21 81 887.3 0 81 22 79 887.3 0 79 23 78 887.3 0 78 24 84 887.3 0 84 25 96 887.3 0 96 26 84 887.3 0 84 27 80 887.3 0 80 28 84 887.3 0 84 29 96 887.3 0 96 30 138 887.3 0 138 31 83 887.3 0 83 32 86 887.3 0 86 33 69 887.3 0 69 34 72 887.3 0 72 35 68 887.3 0 68 36 106 887.3 0 106 37 97 887.3 0 97 38 71 887.3 0 71 39 121 887.3 0 121 40 119 887.3 0 119 41 106 887.3 0 106 42 128 887.3 0 128 43 149 887.3 0 149 44 114 887.3 0 114 45 91 887.3 0 91 46 102 887.3 0 102 47 136 887.3 0 136 48 92 887.3 0 92 49 90 887.3 0 90 50 76 887.3 0 76 51 112 887.3 0 112 52 72 887.3 0 72 53 140 887.3 0 140 54 171 887.3 0 171 55 134 887.3 0 134 56 84 887.3 0 84 57 74 887.3 0 74 58 126 887.3 0 126 59 138 887.3 0 138 60 93 887.3 0 93 61 73 887.3 0 73 62 126 887.3 0 126 63 153 887.3 0 153 64 1037 887.3 0 1037 65 1044 887.3 0 1044 66 988 887.3 0 988 67 84 887.3 0 84 68 159 887.3 0 159 69 97 887.3 0 97 70 157 887.3 0 157 71 111 887.3 0 111 72 70 887.3 0 70 73 60 887.3 0 60 74 86 887.3 0 86 75 110 887.3 0 110 76 91 887.3 0 91 77 69 887.3 0 69 78 61 887.3 0 61 79 109 887.3 0 109 80 123 887.3 0 123 81 126 887.3 0 126 82 63 887.3 0 63 83 99 887.3 0 99 84 96 887.3 0 96 85 84 887.3 0 84 86 76 887.3 0 76 87 90 887.3 0 90 88 64 887.3 0 64 89 139 887.3 0 139 90 153 887.3 0 153 91 186 887.3 0 186 92 502 887.3 0 502 93 376 887.3 0 376 94 283 887.3 0 283 95 151 887.3 0 151 96 84 887.3 0 84 97 73 887.3 0 73 98 67 887.3 0 67 99 94 887.3 0 94 100 129 887.3 0 129 101 152 887.3 0 152 102 99 887.3 0 99 103 82 887.3 0 82 104 108 887.3 0 108 105 121 887.3 0 121 106 97 887.3 0 97 107 67 887.3 0 67 108 81 887.3 0 81 109 92 887.3 0 92 110 86 887.3 0 86 111 116 887.3 0 116 112 90 887.3 0 90 113 63 887.3 0 63 114 103 887.3 0 103 115 103 887.3 0 103 116 96 887.3 0 96 117 165 887.3 0 165 118 105 887.3 0 105 119 107 887.3 0 107 120 90 887.3 0 90 121 88 887.3 0 88 122 117 887.3 0 117 123 121 887.3 0 121 124 102 887.3 0 102 125 102 887.3 0 102 126 76 887.3 0 76 127 106 887.3 0 106 128 109 887.3 0 109 129 105 887.3 0 105 130 259 887.3 0 259 131 259 887.3 0 259 132 190 887.3 0 190 133 145 887.3 0 145 134 95 887.3 0 95 135 172 887.3 0 172 136 221 887.3 0 221 137 188 887.3 0 188 138 135 887.3 0 135 139 123 887.3 0 123 140 90 887.3 0 90 141 104 887.3 0 104 142 109 887.3 0 109 143 120 887.3 0 120 144 104 887.3 0 104 145 110 887.3 0 110 146 115 887.3 0 115 147 179 887.3 0 179 148 183 887.3 0 183 149 176 887.3 0 176 150 170 887.3 0 170 151 153 887.3 0 153 152 191 887.3 0 191 153 203 887.3 0 203 154 143 887.3 0 143 155 309 887.3 0 309 156 544 887.3 0 544 157 362 887.3 0 362 158 315 887.3 0 315 159 133 887.3 0 133 160 186 887.3 0 186 161 139 887.3 0 139 162 156 887.3 0 156 163 158 887.3 0 158 164 109 887.3 0 109 165 112 887.3 0 112 166 159 887.3 0 159 167 228 887.3 0 228 168 307 887.3 0 307 169 241 887.3 0 241 170 120 887.3 0 120 171 250 887.3 0 250 172 222 887.3 0 222 173 212 887.3 0 212 174 93 887.3 0 93 175 319 887.3 0 319 176 612 887.3 0 612 177 460 887.3 0 460 178 155 887.3 0 155 179 154 887.3 0 154 180 120 887.3 0 120 181 151 887.3 0 151 182 183 887.3 0 183 183 208 887.3 0 208 184 129 887.3 0 129 185 133 887.3 0 133 186 158 887.3 0 158 187 176 887.3 0 176 188 322 887.3 0 322 189 223 887.3 0 223 190 166 887.3 0 166 191 135 887.3 0 135 192 99 887.3 0 99 193 112 887.3 0 112 194 112 887.3 0 112 195 156 887.3 0 156 196 161 887.3 0 161 197 150 887.3 0 150 198 155 887.3 0 155 199 141 887.3 0 141 200 185 887.3 0 185 201 220 887.3 0 220 202 228 887.3 0 228 203 157 887.3 0 157 204 181 887.3 0 181 205 153 887.3 0 153 206 114 887.3 0 114 207 154 887.3 0 154 208 148 887.3 0 148 209 130 887.3 0 130 210 182 887.3 0 182 211 151 887.3 0 151 212 178 887.3 0 178 213 147 887.3 0 147 214 204 887.3 0 204 215 232 887.3 0 232 216 349 887.3 0 349 217 338 887.3 0 338 218 329 887.3 0 329 219 333 887.3 0 333 220 421 887.3 0 421 221 851 887.3 0 851 222 1109 887.3 0 1109 223 1147 887.3 0 1147 224 467 887.3 0 467 225 235 887.3 0 235 226 265 887.3 0 265 227 259 887.3 0 259 228 338 887.3 0 338 229 219 887.3 0 219 230 128 887.3 0 128 231 126 887.3 0 126 232 157 887.3 0 157 233 193 887.3 0 193 234 202 887.3 0 202 235 187 887.3 0 187 236 178 887.3 0 178 237 242 887.3 0 242 238 231 887.3 0 231 239 263 887.3 0 263 240 256 887.3 0 256 241 943 887.3 0 943 242 1008 887.3 0 1008 243 1038 887.3 0 1038 244 578 887.3 0 578 245 417 887.3 0 417 246 298 887.3 0 298 247 234 887.3 0 234 248 211 887.3 0 211 249 262 887.3 0 262 250 281 887.3 0 281 251 231 887.3 0 231 252 583 887.3 0 583 253 755 887.3 0 755 254 1183 887.3 0 1183 255 300 887.3 0 300 256 329 887.3 0 329 257 252 887.3 0 252 258 160 887.3 0 160 259 146 887.3 0 146 260 171 887.3 0 171 261 192 887.3 0 192 262 210 887.3 0 210 263 284 887.3 0 284 264 356 887.3 0 356 265 326 887.3 0 326 266 348 887.3 0 348 267 242 887.3 0 242 268 1326 887.3 0 1326 269 1661 887.3 0 1661 270 1690 887.3 0 1690 271 793 887.3 0 793 272 618 887.3 0 618 273 382 887.3 0 382 274 323 887.3 0 323 275 291 887.3 0 291 276 201 887.3 0 201 277 298 887.3 0 298 278 239 887.3 0 239 279 191 887.3 0 191 280 170 887.3 0 170 281 200 887.3 0 200 282 255 887.3 0 255 283 333 887.3 0 333 284 286 887.3 0 286 285 254 887.3 0 254 286 245 887.3 0 245 287 283 887.3 0 283 288 177 887.3 0 177 289 206 887.3 0 206 290 410 887.3 0 410 291 651 887.3 0 651 292 519 887.3 0 519 293 380 887.3 0 380 294 205 887.3 0 205 295 174 887.3 0 174 296 230 887.3 0 230 297 278 887.3 0 278 298 362 887.3 0 362 299 547 887.3 0 547 300 590 887.3 0 590 301 456 887.3 0 456 Finished in 12.73 s (75 us/read; 0.80 M reads/minute). === Summary === Total reads processed: 169,537 Reads with adapters: 76,372 (45.0%) Reads that were too short: 126 (0.1%) Reads written (passing filters): 76,246 (45.0%) Total basepairs processed: 46,624,336 bp Total written (filtered): 21,101,179 bp (45.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 76372 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 12 0.0 2 0 0 0 12 15 56 0.0 3 0 0 38 18 16 507 0.0 3 0 423 50 34 17 74077 0.0 3 70601 2923 449 104 18 150 0.0 3 11 126 5 8 19 1225 0.0 3 983 41 123 78 20 107 0.0 3 38 3 0 66 21 2 0.0 3 2 22 3 0.0 3 0 0 0 3 23 3 0.0 3 0 0 0 3 26 1 0.0 3 0 0 0 1 27 3 0.0 3 3 30 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 36 15 0.0 3 0 0 0 15 37 1 0.0 3 0 0 1 38 3 0.0 3 0 0 0 3 39 4 0.0 3 0 0 0 4 40 1 0.0 3 0 0 0 1 41 9 0.0 3 0 0 1 8 42 2 0.0 3 0 0 1 1 43 1 0.0 3 0 0 0 1 44 4 0.0 3 0 0 0 4 47 1 0.0 3 0 0 0 1 49 2 0.0 3 0 0 1 1 51 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 56 5 0.0 3 0 0 1 4 58 1 0.0 3 0 0 0 1 59 3 0.0 3 0 0 0 3 60 1 0.0 3 0 0 0 1 62 2 0.0 3 0 0 0 2 63 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 67 3 0.0 3 0 0 0 3 73 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 76 2 0.0 3 0 0 0 2 80 1 0.0 3 0 0 0 1 87 2 0.0 3 0 0 0 2 88 1 0.0 3 0 0 0 1 92 3 0.0 3 0 0 0 3 93 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 96 4 0.0 3 0 0 1 3 97 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 0 2 99 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 107 4 0.0 3 0 0 0 4 109 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 2 0.0 3 0 0 0 2 114 1 0.0 3 0 0 0 1 115 2 0.0 3 0 0 0 2 116 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 6 0.0 3 0 0 0 6 128 4 0.0 3 0 0 1 3 129 2 0.0 3 0 0 0 2 132 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 1 137 2 0.0 3 0 0 0 2 139 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 148 3 0.0 3 0 0 0 3 149 4 0.0 3 0 0 0 4 151 2 0.0 3 0 0 0 2 156 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 160 2 0.0 3 0 0 0 2 161 4 0.0 3 0 0 0 4 163 3 0.0 3 0 0 0 3 164 3 0.0 3 0 0 0 3 166 5 0.0 3 0 0 0 5 167 3 0.0 3 0 0 1 2 172 1 0.0 3 0 1 177 3 0.0 3 0 0 1 2 181 1 0.0 3 0 0 0 1 182 2 0.0 3 0 0 0 2 183 8 0.0 3 0 0 0 8 184 4 0.0 3 0 0 0 4 185 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 1 187 1 0.0 3 0 0 1 188 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 216 2 0.0 3 0 0 0 2 217 16 0.0 3 0 0 1 15 219 1 0.0 3 0 0 0 1 221 3 0.0 3 0 0 1 2 224 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 235 2 0.0 3 0 0 0 2 238 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 279 4 0.0 3 0 0 0 4 281 9 0.0 3 0 0 0 9 282 2 0.0 3 0 0 0 2 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.45 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 76,246 Reads with adapters: 1,302 (1.7%) Reads written (passing filters): 76,246 (100.0%) Total basepairs processed: 21,101,179 bp Total written (filtered): 21,097,252 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1302 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 13.6% C: 76.3% G: 8.6% T: 1.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1287 1191.3 0 1287 4 10 297.8 0 10 5 4 74.5 0 4 6 1 18.6 0 1