This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTCAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 177.69 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 178,355 (4.9%) Reads that were too short: 17,419 (0.5%) Reads written (passing filters): 160,936 (4.4%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 45,489,431 bp (4.2%) === Adapter 1 === Sequence: CGTCAC; Type: regular 5'; Length: 6; Trimmed: 178355 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2425 887.3 0 2425 7 65417 887.3 0 65417 8 75645 887.3 0 75645 9 241 887.3 0 241 10 64 887.3 0 64 11 49 887.3 0 49 12 47 887.3 0 47 13 60 887.3 0 60 14 60 887.3 0 60 15 39 887.3 0 39 16 52 887.3 0 52 17 54 887.3 0 54 18 64 887.3 0 64 19 48 887.3 0 48 20 42 887.3 0 42 21 35 887.3 0 35 22 41 887.3 0 41 23 46 887.3 0 46 24 58 887.3 0 58 25 46 887.3 0 46 26 54 887.3 0 54 27 49 887.3 0 49 28 66 887.3 0 66 29 65 887.3 0 65 30 54 887.3 0 54 31 70 887.3 0 70 32 435 887.3 0 435 33 50 887.3 0 50 34 41 887.3 0 41 35 53 887.3 0 53 36 44 887.3 0 44 37 68 887.3 0 68 38 52 887.3 0 52 39 76 887.3 0 76 40 65 887.3 0 65 41 59 887.3 0 59 42 58 887.3 0 58 43 132 887.3 0 132 44 66 887.3 0 66 45 58 887.3 0 58 46 78 887.3 0 78 47 124 887.3 0 124 48 51 887.3 0 51 49 57 887.3 0 57 50 73 887.3 0 73 51 65 887.3 0 65 52 53 887.3 0 53 53 65 887.3 0 65 54 93 887.3 0 93 55 62 887.3 0 62 56 37 887.3 0 37 57 62 887.3 0 62 58 56 887.3 0 56 59 49 887.3 0 49 60 56 887.3 0 56 61 50 887.3 0 50 62 84 887.3 0 84 63 85 887.3 0 85 64 642 887.3 0 642 65 699 887.3 0 699 66 827 887.3 0 827 67 56 887.3 0 56 68 123 887.3 0 123 69 45 887.3 0 45 70 61 887.3 0 61 71 76 887.3 0 76 72 36 887.3 0 36 73 53 887.3 0 53 74 58 887.3 0 58 75 55 887.3 0 55 76 34 887.3 0 34 77 57 887.3 0 57 78 51 887.3 0 51 79 45 887.3 0 45 80 65 887.3 0 65 81 74 887.3 0 74 82 50 887.3 0 50 83 64 887.3 0 64 84 68 887.3 0 68 85 49 887.3 0 49 86 54 887.3 0 54 87 51 887.3 0 51 88 48 887.3 0 48 89 64 887.3 0 64 90 95 887.3 0 95 91 117 887.3 0 117 92 270 887.3 0 270 93 243 887.3 0 243 94 204 887.3 0 204 95 59 887.3 0 59 96 42 887.3 0 42 97 54 887.3 0 54 98 46 887.3 0 46 99 54 887.3 0 54 100 49 887.3 0 49 101 81 887.3 0 81 102 68 887.3 0 68 103 56 887.3 0 56 104 65 887.3 0 65 105 61 887.3 0 61 106 54 887.3 0 54 107 40 887.3 0 40 108 76 887.3 0 76 109 64 887.3 0 64 110 59 887.3 0 59 111 71 887.3 0 71 112 58 887.3 0 58 113 36 887.3 0 36 114 52 887.3 0 52 115 65 887.3 0 65 116 44 887.3 0 44 117 68 887.3 0 68 118 90 887.3 0 90 119 63 887.3 0 63 120 53 887.3 0 53 121 66 887.3 0 66 122 81 887.3 0 81 123 68 887.3 0 68 124 71 887.3 0 71 125 82 887.3 0 82 126 44 887.3 0 44 127 80 887.3 0 80 128 69 887.3 0 69 129 60 887.3 0 60 130 98 887.3 0 98 131 81 887.3 0 81 132 82 887.3 0 82 133 70 887.3 0 70 134 54 887.3 0 54 135 66 887.3 0 66 136 101 887.3 0 101 137 72 887.3 0 72 138 73 887.3 0 73 139 97 887.3 0 97 140 70 887.3 0 70 141 60 887.3 0 60 142 60 887.3 0 60 143 65 887.3 0 65 144 73 887.3 0 73 145 54 887.3 0 54 146 85 887.3 0 85 147 84 887.3 0 84 148 95 887.3 0 95 149 116 887.3 0 116 150 160 887.3 0 160 151 87 887.3 0 87 152 80 887.3 0 80 153 171 887.3 0 171 154 78 887.3 0 78 155 116 887.3 0 116 156 245 887.3 0 245 157 159 887.3 0 159 158 130 887.3 0 130 159 96 887.3 0 96 160 108 887.3 0 108 161 71 887.3 0 71 162 84 887.3 0 84 163 61 887.3 0 61 164 73 887.3 0 73 165 61 887.3 0 61 166 69 887.3 0 69 167 125 887.3 0 125 168 125 887.3 0 125 169 119 887.3 0 119 170 53 887.3 0 53 171 124 887.3 0 124 172 112 887.3 0 112 173 107 887.3 0 107 174 57 887.3 0 57 175 181 887.3 0 181 176 300 887.3 0 300 177 215 887.3 0 215 178 91 887.3 0 91 179 73 887.3 0 73 180 70 887.3 0 70 181 91 887.3 0 91 182 85 887.3 0 85 183 97 887.3 0 97 184 69 887.3 0 69 185 76 887.3 0 76 186 102 887.3 0 102 187 98 887.3 0 98 188 151 887.3 0 151 189 109 887.3 0 109 190 109 887.3 0 109 191 99 887.3 0 99 192 73 887.3 0 73 193 70 887.3 0 70 194 74 887.3 0 74 195 62 887.3 0 62 196 83 887.3 0 83 197 71 887.3 0 71 198 89 887.3 0 89 199 97 887.3 0 97 200 71 887.3 0 71 201 72 887.3 0 72 202 86 887.3 0 86 203 90 887.3 0 90 204 100 887.3 0 100 205 110 887.3 0 110 206 90 887.3 0 90 207 85 887.3 0 85 208 84 887.3 0 84 209 66 887.3 0 66 210 87 887.3 0 87 211 99 887.3 0 99 212 110 887.3 0 110 213 111 887.3 0 111 214 102 887.3 0 102 215 97 887.3 0 97 216 135 887.3 0 135 217 149 887.3 0 149 218 109 887.3 0 109 219 87 887.3 0 87 220 136 887.3 0 136 221 235 887.3 0 235 222 260 887.3 0 260 223 250 887.3 0 250 224 187 887.3 0 187 225 136 887.3 0 136 226 141 887.3 0 141 227 147 887.3 0 147 228 188 887.3 0 188 229 100 887.3 0 100 230 75 887.3 0 75 231 80 887.3 0 80 232 85 887.3 0 85 233 99 887.3 0 99 234 139 887.3 0 139 235 116 887.3 0 116 236 112 887.3 0 112 237 141 887.3 0 141 238 122 887.3 0 122 239 138 887.3 0 138 240 106 887.3 0 106 241 343 887.3 0 343 242 304 887.3 0 304 243 336 887.3 0 336 244 227 887.3 0 227 245 187 887.3 0 187 246 168 887.3 0 168 247 158 887.3 0 158 248 134 887.3 0 134 249 123 887.3 0 123 250 158 887.3 0 158 251 133 887.3 0 133 252 289 887.3 0 289 253 371 887.3 0 371 254 537 887.3 0 537 255 128 887.3 0 128 256 145 887.3 0 145 257 134 887.3 0 134 258 91 887.3 0 91 259 74 887.3 0 74 260 80 887.3 0 80 261 98 887.3 0 98 262 104 887.3 0 104 263 108 887.3 0 108 264 140 887.3 0 140 265 149 887.3 0 149 266 189 887.3 0 189 267 130 887.3 0 130 268 662 887.3 0 662 269 1015 887.3 0 1015 270 663 887.3 0 663 271 217 887.3 0 217 272 200 887.3 0 200 273 175 887.3 0 175 274 205 887.3 0 205 275 161 887.3 0 161 276 119 887.3 0 119 277 228 887.3 0 228 278 143 887.3 0 143 279 112 887.3 0 112 280 112 887.3 0 112 281 110 887.3 0 110 282 120 887.3 0 120 283 153 887.3 0 153 284 102 887.3 0 102 285 126 887.3 0 126 286 147 887.3 0 147 287 153 887.3 0 153 288 111 887.3 0 111 289 114 887.3 0 114 290 165 887.3 0 165 291 215 887.3 0 215 292 232 887.3 0 232 293 166 887.3 0 166 294 104 887.3 0 104 295 131 887.3 0 131 296 142 887.3 0 142 297 198 887.3 0 198 298 252 887.3 0 252 299 283 887.3 0 283 300 315 887.3 0 315 301 240 887.3 0 240 Finished in 12.92 s (80 us/read; 0.75 M reads/minute). === Summary === Total reads processed: 160,936 Reads with adapters: 79,242 (49.2%) Reads that were too short: 25 (0.0%) Reads written (passing filters): 79,217 (49.2%) Total basepairs processed: 45,489,431 bp Total written (filtered): 21,507,515 bp (47.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 79242 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 24 0.0 3 0 0 23 1 19 597 0.0 3 0 579 15 2 1 20 76482 0.0 4 75197 1234 46 3 2 21 221 0.0 4 45 171 4 1 22 31 0.0 4 1 12 17 1 23 691 0.0 4 640 10 0 39 2 24 10 0.0 4 3 3 0 2 2 25 1 0.0 4 0 0 1 26 2 0.0 4 1 0 0 1 28 2 0.0 4 1 1 35 23 0.0 4 0 0 0 0 23 56 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 94 2 0.0 4 0 0 0 0 2 104 1 0.0 4 1 107 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 126 10 0.0 4 0 0 0 0 10 127 1105 0.0 4 0 0 0 0 1105 128 1 0.0 4 0 0 0 0 1 129 6 0.0 4 0 0 0 0 6 130 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 152 2 0.0 4 0 0 0 0 2 153 1 0.0 4 0 0 0 0 1 155 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 1 198 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 229 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 245 1 0.0 4 0 0 0 0 1 254 1 0.0 4 1 262 1 0.0 4 0 0 0 0 1 265 2 0.0 4 0 0 0 0 2 267 1 0.0 4 0 0 0 0 1 282 4 0.0 4 0 0 0 0 4 283 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.77 s (60 us/read; 1.00 M reads/minute). === Summary === Total reads processed: 79,217 Reads with adapters: 7,236 (9.1%) Reads written (passing filters): 79,217 (100.0%) Total basepairs processed: 21,507,515 bp Total written (filtered): 21,424,173 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7236 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.2% C: 2.8% G: 90.8% T: 1.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 34 1237.8 0 34 4 24 309.4 0 24 5 6 77.4 0 6 6 3 19.3 0 3 8 3 1.2 0 3 9 132 0.3 0 3 129 10 1132 0.1 1 35 1097 11 5565 0.0 1 39 5526 12 2 0.0 1 0 2 14 2 0.0 1 0 2 18 1 0.0 1 0 1 19 229 0.0 1 191 38 20 34 0.0 1 28 6 21 2 0.0 1 2 22 3 0.0 1 2 1 23 31 0.0 1 23 8 24 4 0.0 1 2 2 25 1 0.0 1 1 46 1 0.0 1 1 115 23 0.0 1 22 1 151 3 0.0 1 0 3 162 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.