This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATAATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 173.78 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 715,460 (19.7%) Reads that were too short: 611,197 (16.8%) Reads written (passing filters): 104,263 (2.9%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 26,189,734 bp (2.4%) === Adapter 1 === Sequence: ATAATT; Type: regular 5'; Length: 6; Trimmed: 715460 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 563 887.3 0 563 7 38831 887.3 0 38831 8 840 887.3 0 840 9 28741 887.3 0 28741 10 102 887.3 0 102 11 80 887.3 0 80 12 64 887.3 0 64 13 92 887.3 0 92 14 68 887.3 0 68 15 72 887.3 0 72 16 73 887.3 0 73 17 61 887.3 0 61 18 75 887.3 0 75 19 68 887.3 0 68 20 60 887.3 0 60 21 64 887.3 0 64 22 47 887.3 0 47 23 61 887.3 0 61 24 63 887.3 0 63 25 57 887.3 0 57 26 59 887.3 0 59 27 60 887.3 0 60 28 92 887.3 0 92 29 65 887.3 0 65 30 62 887.3 0 62 31 77 887.3 0 77 32 54 887.3 0 54 33 56 887.3 0 56 34 48 887.3 0 48 35 43 887.3 0 43 36 57 887.3 0 57 37 55 887.3 0 55 38 81 887.3 0 81 39 44 887.3 0 44 40 47 887.3 0 47 41 72 887.3 0 72 42 54 887.3 0 54 43 65 887.3 0 65 44 418 887.3 0 418 45 73 887.3 0 73 46 42 887.3 0 42 47 57 887.3 0 57 48 62 887.3 0 62 49 58 887.3 0 58 50 59 887.3 0 59 51 54 887.3 0 54 52 61 887.3 0 61 53 53 887.3 0 53 54 49 887.3 0 49 55 42 887.3 0 42 56 33 887.3 0 33 57 49 887.3 0 49 58 55 887.3 0 55 59 76 887.3 0 76 60 75 887.3 0 75 61 45 887.3 0 45 62 55 887.3 0 55 63 39 887.3 0 39 64 52 887.3 0 52 65 51 887.3 0 51 66 64 887.3 0 64 67 66 887.3 0 66 68 91 887.3 0 91 69 131 887.3 0 131 70 320 887.3 0 320 71 57 887.3 0 57 72 73 887.3 0 73 73 179 887.3 0 179 74 54 887.3 0 54 75 63 887.3 0 63 76 77 887.3 0 77 77 87 887.3 0 87 78 83 887.3 0 83 79 107 887.3 0 107 80 159 887.3 0 159 81 148 887.3 0 148 82 137 887.3 0 137 83 130 887.3 0 130 84 148 887.3 0 148 85 166 887.3 0 166 86 247 887.3 0 247 87 191 887.3 0 191 88 213 887.3 0 213 89 145 887.3 0 145 90 118 887.3 0 118 91 119 887.3 0 119 92 119 887.3 0 119 93 149 887.3 0 149 94 158 887.3 0 158 95 136 887.3 0 136 96 185 887.3 0 185 97 141 887.3 0 141 98 152 887.3 0 152 99 173 887.3 0 173 100 139 887.3 0 139 101 150 887.3 0 150 102 128 887.3 0 128 103 108 887.3 0 108 104 110 887.3 0 110 105 139 887.3 0 139 106 161 887.3 0 161 107 163 887.3 0 163 108 137 887.3 0 137 109 135 887.3 0 135 110 128 887.3 0 128 111 177 887.3 0 177 112 143 887.3 0 143 113 121 887.3 0 121 114 129 887.3 0 129 115 129 887.3 0 129 116 108 887.3 0 108 117 96 887.3 0 96 118 131 887.3 0 131 119 544 887.3 0 544 120 152 887.3 0 152 121 190 887.3 0 190 122 230 887.3 0 230 123 156 887.3 0 156 124 229 887.3 0 229 125 198 887.3 0 198 126 130 887.3 0 130 127 159 887.3 0 159 128 137 887.3 0 137 129 137 887.3 0 137 130 134 887.3 0 134 131 137 887.3 0 137 132 174 887.3 0 174 133 200 887.3 0 200 134 149 887.3 0 149 135 148 887.3 0 148 136 113 887.3 0 113 137 122 887.3 0 122 138 178 887.3 0 178 139 346 887.3 0 346 140 555 887.3 0 555 141 304 887.3 0 304 142 132 887.3 0 132 143 109 887.3 0 109 144 130 887.3 0 130 145 224 887.3 0 224 146 414 887.3 0 414 147 242 887.3 0 242 148 484 887.3 0 484 149 103 887.3 0 103 150 81 887.3 0 81 151 93 887.3 0 93 152 81 887.3 0 81 153 126 887.3 0 126 154 155 887.3 0 155 155 201 887.3 0 201 156 186 887.3 0 186 157 215 887.3 0 215 158 237 887.3 0 237 159 1985 887.3 0 1985 160 5244 887.3 0 5244 161 3656 887.3 0 3656 162 118 887.3 0 118 163 101 887.3 0 101 164 104 887.3 0 104 165 124 887.3 0 124 166 121 887.3 0 121 167 169 887.3 0 169 168 155 887.3 0 155 169 119 887.3 0 119 170 115 887.3 0 115 171 95 887.3 0 95 172 145 887.3 0 145 173 180 887.3 0 180 174 179 887.3 0 179 175 163 887.3 0 163 176 133 887.3 0 133 177 130 887.3 0 130 178 92 887.3 0 92 179 98 887.3 0 98 180 116 887.3 0 116 181 130 887.3 0 130 182 108 887.3 0 108 183 118 887.3 0 118 184 137 887.3 0 137 185 130 887.3 0 130 186 174 887.3 0 174 187 157 887.3 0 157 188 111 887.3 0 111 189 104 887.3 0 104 190 111 887.3 0 111 191 115 887.3 0 115 192 148 887.3 0 148 193 159 887.3 0 159 194 198 887.3 0 198 195 142 887.3 0 142 196 160 887.3 0 160 197 185 887.3 0 185 198 175 887.3 0 175 199 167 887.3 0 167 200 161 887.3 0 161 201 147 887.3 0 147 202 152 887.3 0 152 203 164 887.3 0 164 204 144 887.3 0 144 205 171 887.3 0 171 206 245 887.3 0 245 207 307 887.3 0 307 208 274 887.3 0 274 209 311 887.3 0 311 210 794 887.3 0 794 211 487 887.3 0 487 212 641 887.3 0 641 213 527 887.3 0 527 214 388 887.3 0 388 215 159 887.3 0 159 216 154 887.3 0 154 217 191 887.3 0 191 218 412 887.3 0 412 219 264 887.3 0 264 220 246 887.3 0 246 221 237 887.3 0 237 222 206 887.3 0 206 223 193 887.3 0 193 224 195 887.3 0 195 225 282 887.3 0 282 226 197 887.3 0 197 227 447 887.3 0 447 228 1181 887.3 0 1181 229 238 887.3 0 238 230 169 887.3 0 169 231 164 887.3 0 164 232 313 887.3 0 313 233 331 887.3 0 331 234 287 887.3 0 287 235 298 887.3 0 298 236 233 887.3 0 233 237 565 887.3 0 565 238 1285 887.3 0 1285 239 269 887.3 0 269 240 390 887.3 0 390 241 258 887.3 0 258 242 389 887.3 0 389 243 695 887.3 0 695 244 304 887.3 0 304 245 378 887.3 0 378 246 212 887.3 0 212 247 459 887.3 0 459 248 835 887.3 0 835 249 1034 887.3 0 1034 250 1834 887.3 0 1834 251 2488 887.3 0 2488 252 3475 887.3 0 3475 253 5575 887.3 0 5575 254 22409 887.3 0 22409 255 150883 887.3 0 150883 256 221205 887.3 0 221205 257 175737 887.3 0 175737 258 3935 887.3 0 3935 259 284 887.3 0 284 260 182 887.3 0 182 261 280 887.3 0 280 262 186 887.3 0 186 263 132 887.3 0 132 264 96 887.3 0 96 265 114 887.3 0 114 266 162 887.3 0 162 267 322 887.3 0 322 268 149 887.3 0 149 269 69 887.3 0 69 270 69 887.3 0 69 271 74 887.3 0 74 272 95 887.3 0 95 273 96 887.3 0 96 274 110 887.3 0 110 275 81 887.3 0 81 276 81 887.3 0 81 277 92 887.3 0 92 278 106 887.3 0 106 279 116 887.3 0 116 280 110 887.3 0 110 281 96 887.3 0 96 282 108 887.3 0 108 283 135 887.3 0 135 284 127 887.3 0 127 285 79 887.3 0 79 286 84 887.3 0 84 287 69 887.3 0 69 288 70 887.3 0 70 289 69 887.3 0 69 290 85 887.3 0 85 291 89 887.3 0 89 292 87 887.3 0 87 293 79 887.3 0 79 294 115 887.3 0 115 295 314 887.3 0 314 296 91 887.3 0 91 297 92 887.3 0 92 298 126 887.3 0 126 299 290 887.3 0 290 300 169 887.3 0 169 301 201 887.3 0 201 Finished in 7.84 s (75 us/read; 0.80 M reads/minute). === Summary === Total reads processed: 104,263 Reads with adapters: 41,437 (39.7%) Reads that were too short: 22 (0.0%) Reads written (passing filters): 41,415 (39.7%) Total basepairs processed: 26,189,734 bp Total written (filtered): 11,083,335 bp (42.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 41437 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 6 0.0 3 0 0 6 19 245 0.0 3 0 242 1 2 20 38591 0.0 4 37894 667 23 4 3 21 114 0.0 4 18 94 1 0 1 22 10 0.0 4 1 1 7 0 1 23 27 0.0 4 0 0 0 22 5 26 1 0.0 4 0 0 0 0 1 27 1 0.0 4 1 28 1 0.0 4 0 0 0 0 1 30 1 0.0 4 1 32 1 0.0 4 0 0 0 0 1 33 1 0.0 4 1 62 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 115 2 0.0 4 2 116 1 0.0 4 1 125 4 0.0 4 0 0 0 0 4 126 27 0.0 4 1 0 0 0 26 127 2280 0.0 4 0 0 0 0 2280 128 3 0.0 4 0 0 0 0 3 129 1 0.0 4 0 0 0 1 130 2 0.0 4 0 0 0 0 2 134 1 0.0 4 0 0 0 0 1 135 98 0.0 4 0 0 0 0 98 153 2 0.0 4 0 0 0 0 2 165 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 4 0.0 4 0 0 0 0 4 229 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 253 1 0.0 4 0 0 1 263 1 0.0 4 0 0 0 0 1 275 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.29 s (55 us/read; 1.09 M reads/minute). === Summary === Total reads processed: 41,415 Reads with adapters: 474 (1.1%) Reads written (passing filters): 41,415 (100.0%) Total basepairs processed: 11,083,335 bp Total written (filtered): 11,078,497 bp (100.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 474 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 15.4% C: 7.6% G: 74.1% T: 3.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 87 647.1 0 87 4 7 161.8 0 7 5 3 40.4 0 3 9 2 0.2 0 0 2 10 6 0.0 1 0 6 11 287 0.0 1 3 284 12 75 0.0 1 2 73 13 2 0.0 1 0 2 15 2 0.0 1 0 2 39 1 0.0 1 1 152 2 0.0 1 0 2