This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTAAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 175.33 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 263,017 (7.2%) Reads that were too short: 45,879 (1.3%) Reads written (passing filters): 217,138 (6.0%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 52,889,893 bp (4.8%) === Adapter 1 === Sequence: GGTAAG; Type: regular 5'; Length: 6; Trimmed: 263017 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 5435 887.3 0 5435 7 2643 887.3 0 2643 8 80214 887.3 0 80214 9 60118 887.3 0 60118 10 122 887.3 0 122 11 50 887.3 0 50 12 49 887.3 0 49 13 40 887.3 0 40 14 48 887.3 0 48 15 48 887.3 0 48 16 44 887.3 0 44 17 64 887.3 0 64 18 65 887.3 0 65 19 47 887.3 0 47 20 47 887.3 0 47 21 83 887.3 0 83 22 82 887.3 0 82 23 94 887.3 0 94 24 43 887.3 0 43 25 44 887.3 0 44 26 174 887.3 0 174 27 92 887.3 0 92 28 64 887.3 0 64 29 85 887.3 0 85 30 59 887.3 0 59 31 59 887.3 0 59 32 54 887.3 0 54 33 72 887.3 0 72 34 88 887.3 0 88 35 57 887.3 0 57 36 54 887.3 0 54 37 72 887.3 0 72 38 81 887.3 0 81 39 32 887.3 0 32 40 61 887.3 0 61 41 59 887.3 0 59 42 53 887.3 0 53 43 55 887.3 0 55 44 45 887.3 0 45 45 51 887.3 0 51 46 35 887.3 0 35 47 68 887.3 0 68 48 51 887.3 0 51 49 44 887.3 0 44 50 70 887.3 0 70 51 77 887.3 0 77 52 51 887.3 0 51 53 60 887.3 0 60 54 62 887.3 0 62 55 58 887.3 0 58 56 39 887.3 0 39 57 47 887.3 0 47 58 43 887.3 0 43 59 51 887.3 0 51 60 46 887.3 0 46 61 63 887.3 0 63 62 42 887.3 0 42 63 46 887.3 0 46 64 55 887.3 0 55 65 84 887.3 0 84 66 71 887.3 0 71 67 59 887.3 0 59 68 88 887.3 0 88 69 135 887.3 0 135 70 168 887.3 0 168 71 161 887.3 0 161 72 56 887.3 0 56 73 56 887.3 0 56 74 60 887.3 0 60 75 80 887.3 0 80 76 144 887.3 0 144 77 105 887.3 0 105 78 70 887.3 0 70 79 163 887.3 0 163 80 218 887.3 0 218 81 238 887.3 0 238 82 99 887.3 0 99 83 45 887.3 0 45 84 59 887.3 0 59 85 59 887.3 0 59 86 59 887.3 0 59 87 49 887.3 0 49 88 62 887.3 0 62 89 136 887.3 0 136 90 61 887.3 0 61 91 71 887.3 0 71 92 66 887.3 0 66 93 62 887.3 0 62 94 63 887.3 0 63 95 82 887.3 0 82 96 83 887.3 0 83 97 82 887.3 0 82 98 67 887.3 0 67 99 93 887.3 0 93 100 111 887.3 0 111 101 137 887.3 0 137 102 54 887.3 0 54 103 61 887.3 0 61 104 61 887.3 0 61 105 53 887.3 0 53 106 45 887.3 0 45 107 73 887.3 0 73 108 57 887.3 0 57 109 52 887.3 0 52 110 69 887.3 0 69 111 65 887.3 0 65 112 132 887.3 0 132 113 183 887.3 0 183 114 146 887.3 0 146 115 79 887.3 0 79 116 84 887.3 0 84 117 684 887.3 0 684 118 296 887.3 0 296 119 172 887.3 0 172 120 82 887.3 0 82 121 59 887.3 0 59 122 72 887.3 0 72 123 77 887.3 0 77 124 56 887.3 0 56 125 76 887.3 0 76 126 90 887.3 0 90 127 96 887.3 0 96 128 99 887.3 0 99 129 104 887.3 0 104 130 119 887.3 0 119 131 401 887.3 0 401 132 335 887.3 0 335 133 211 887.3 0 211 134 137 887.3 0 137 135 122 887.3 0 122 136 211 887.3 0 211 137 438 887.3 0 438 138 217 887.3 0 217 139 191 887.3 0 191 140 130 887.3 0 130 141 66 887.3 0 66 142 69 887.3 0 69 143 91 887.3 0 91 144 93 887.3 0 93 145 101 887.3 0 101 146 116 887.3 0 116 147 101 887.3 0 101 148 85 887.3 0 85 149 60 887.3 0 60 150 72 887.3 0 72 151 66 887.3 0 66 152 71 887.3 0 71 153 93 887.3 0 93 154 132 887.3 0 132 155 105 887.3 0 105 156 644 887.3 0 644 157 158 887.3 0 158 158 141 887.3 0 141 159 91 887.3 0 91 160 84 887.3 0 84 161 101 887.3 0 101 162 84 887.3 0 84 163 95 887.3 0 95 164 122 887.3 0 122 165 211 887.3 0 211 166 104 887.3 0 104 167 132 887.3 0 132 168 214 887.3 0 214 169 163 887.3 0 163 170 159 887.3 0 159 171 220 887.3 0 220 172 181 887.3 0 181 173 225 887.3 0 225 174 270 887.3 0 270 175 525 887.3 0 525 176 873 887.3 0 873 177 1239 887.3 0 1239 178 8563 887.3 0 8563 179 5140 887.3 0 5140 180 15641 887.3 0 15641 181 4555 887.3 0 4555 182 6189 887.3 0 6189 183 1863 887.3 0 1863 184 280 887.3 0 280 185 150 887.3 0 150 186 74 887.3 0 74 187 62 887.3 0 62 188 185 887.3 0 185 189 131 887.3 0 131 190 92 887.3 0 92 191 109 887.3 0 109 192 115 887.3 0 115 193 117 887.3 0 117 194 209 887.3 0 209 195 375 887.3 0 375 196 1292 887.3 0 1292 197 1514 887.3 0 1514 198 917 887.3 0 917 199 321 887.3 0 321 200 233 887.3 0 233 201 708 887.3 0 708 202 1125 887.3 0 1125 203 920 887.3 0 920 204 575 887.3 0 575 205 62 887.3 0 62 206 66 887.3 0 66 207 72 887.3 0 72 208 65 887.3 0 65 209 77 887.3 0 77 210 78 887.3 0 78 211 70 887.3 0 70 212 80 887.3 0 80 213 75 887.3 0 75 214 71 887.3 0 71 215 138 887.3 0 138 216 197 887.3 0 197 217 243 887.3 0 243 218 117 887.3 0 117 219 97 887.3 0 97 220 99 887.3 0 99 221 176 887.3 0 176 222 595 887.3 0 595 223 799 887.3 0 799 224 464 887.3 0 464 225 366 887.3 0 366 226 73 887.3 0 73 227 113 887.3 0 113 228 86 887.3 0 86 229 67 887.3 0 67 230 73 887.3 0 73 231 83 887.3 0 83 232 75 887.3 0 75 233 107 887.3 0 107 234 385 887.3 0 385 235 350 887.3 0 350 236 574 887.3 0 574 237 212 887.3 0 212 238 247 887.3 0 247 239 1543 887.3 0 1543 240 643 887.3 0 643 241 799 887.3 0 799 242 546 887.3 0 546 243 687 887.3 0 687 244 369 887.3 0 369 245 162 887.3 0 162 246 96 887.3 0 96 247 96 887.3 0 96 248 82 887.3 0 82 249 58 887.3 0 58 250 97 887.3 0 97 251 128 887.3 0 128 252 172 887.3 0 172 253 188 887.3 0 188 254 248 887.3 0 248 255 120 887.3 0 120 256 317 887.3 0 317 257 488 887.3 0 488 258 565 887.3 0 565 259 1324 887.3 0 1324 260 1143 887.3 0 1143 261 878 887.3 0 878 262 250 887.3 0 250 263 329 887.3 0 329 264 2199 887.3 0 2199 265 4725 887.3 0 4725 266 4407 887.3 0 4407 267 1349 887.3 0 1349 268 57 887.3 0 57 269 84 887.3 0 84 270 92 887.3 0 92 271 89 887.3 0 89 272 88 887.3 0 88 273 118 887.3 0 118 274 184 887.3 0 184 275 141 887.3 0 141 276 297 887.3 0 297 277 229 887.3 0 229 278 361 887.3 0 361 279 164 887.3 0 164 280 69 887.3 0 69 281 51 887.3 0 51 282 69 887.3 0 69 283 74 887.3 0 74 284 95 887.3 0 95 285 81 887.3 0 81 286 108 887.3 0 108 287 114 887.3 0 114 288 118 887.3 0 118 289 142 887.3 0 142 290 260 887.3 0 260 291 3593 887.3 0 3593 292 2391 887.3 0 2391 293 2048 887.3 0 2048 294 338 887.3 0 338 295 231 887.3 0 231 296 136 887.3 0 136 297 174 887.3 0 174 298 171 887.3 0 171 299 272 887.3 0 272 300 196 887.3 0 196 301 164 887.3 0 164 Finished in 15.21 s (70 us/read; 0.86 M reads/minute). === Summary === Total reads processed: 217,138 Reads with adapters: 84,189 (38.8%) Reads that were too short: 191 (0.1%) Reads written (passing filters): 83,998 (38.7%) Total basepairs processed: 52,889,893 bp Total written (filtered): 23,154,642 bp (43.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 84189 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 11 0.0 2 0 0 0 11 15 47 0.0 3 0 0 29 18 16 706 0.0 3 0 639 38 29 17 78018 0.0 3 74015 3384 501 118 18 257 0.0 3 34 198 16 9 19 211 0.0 3 11 27 100 73 20 4548 0.0 3 4245 221 32 50 21 13 0.0 3 4 7 1 1 22 11 0.0 3 0 0 9 2 23 8 0.0 3 0 0 0 8 24 1 0.0 3 0 0 1 27 2 0.0 3 0 0 0 2 28 2 0.0 3 1 0 0 1 29 1 0.0 3 1 30 3 0.0 3 0 0 0 3 33 3 0.0 3 0 0 0 3 34 1 0.0 3 0 0 0 1 35 9 0.0 3 1 0 0 8 36 1 0.0 3 0 0 0 1 38 2 0.0 3 0 0 0 2 40 1 0.0 3 1 41 11 0.0 3 0 0 0 11 45 2 0.0 3 0 0 0 2 47 1 0.0 3 0 0 0 1 49 8 0.0 3 0 0 0 8 50 2 0.0 3 0 0 0 2 54 22 0.0 3 0 0 0 22 56 2 0.0 3 0 0 0 2 57 10 0.0 3 0 0 0 10 59 3 0.0 3 0 0 0 3 60 28 0.0 3 0 0 0 28 61 1 0.0 3 0 0 0 1 63 11 0.0 3 0 0 0 11 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 71 2 0.0 3 0 0 0 2 72 2 0.0 3 0 0 0 2 73 1 0.0 3 0 0 0 1 74 11 0.0 3 0 0 0 11 75 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 77 2 0.0 3 0 0 0 2 78 2 0.0 3 0 0 0 2 79 1 0.0 3 0 0 0 1 80 9 0.0 3 0 0 0 9 81 3 0.0 3 0 0 0 3 82 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 91 5 0.0 3 0 0 0 5 92 11 0.0 3 0 0 0 11 93 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 1 96 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 99 2 0.0 3 0 0 0 2 102 26 0.0 3 0 0 1 25 103 7 0.0 3 0 0 0 7 104 4 0.0 3 0 0 0 4 105 5 0.0 3 0 0 1 4 106 2 0.0 3 0 0 0 2 108 1 0.0 3 0 0 0 1 110 6 0.0 3 0 0 0 6 111 3 0.0 3 0 0 0 3 112 3 0.0 3 0 0 0 3 114 2 0.0 3 0 0 0 2 115 4 0.0 3 0 0 0 4 116 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 1 119 2 0.0 3 0 0 0 2 120 3 0.0 3 0 0 0 3 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 1 127 2 0.0 3 0 0 0 2 128 1 0.0 3 0 0 0 1 135 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 140 3 0.0 3 0 0 0 3 141 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 143 2 0.0 3 0 0 0 2 148 1 0.0 3 0 0 0 1 149 2 0.0 3 0 0 0 2 150 2 0.0 3 0 0 0 2 151 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 158 2 0.0 3 0 0 0 2 159 7 0.0 3 0 0 1 6 161 5 0.0 3 0 0 0 5 163 3 0.0 3 0 0 0 3 167 3 0.0 3 0 1 1 1 168 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 2 0.0 3 0 1 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 1 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 213 3 0.0 3 0 0 0 3 214 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 227 2 0.0 3 0 0 0 2 238 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 274 1 0.0 3 0 0 1 279 5 0.0 3 0 0 0 5 280 4 0.0 3 0 0 0 4 281 3 0.0 3 0 0 0 3 282 4 0.0 3 0 0 0 4 286 1 0.0 3 0 0 0 1 288 2 0.0 3 0 0 0 2 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.88 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 83,998 Reads with adapters: 375 (0.4%) Reads written (passing filters): 83,998 (100.0%) Total basepairs processed: 23,154,642 bp Total written (filtered): 23,153,462 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 375 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 49.1% C: 23.2% G: 19.5% T: 8.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 330 1312.5 0 330 4 35 328.1 0 35 5 10 82.0 0 10