This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTAAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 174.05 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 243,586 (6.7%) Reads that were too short: 33,001 (0.9%) Reads written (passing filters): 210,585 (5.8%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 52,457,838 bp (4.8%) === Adapter 1 === Sequence: GGTAAG; Type: regular 5'; Length: 6; Trimmed: 243586 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 5344 887.3 0 5344 7 1951 887.3 0 1951 8 67051 887.3 0 67051 9 78729 887.3 0 78729 10 119 887.3 0 119 11 30 887.3 0 30 12 31 887.3 0 31 13 24 887.3 0 24 14 31 887.3 0 31 15 26 887.3 0 26 16 37 887.3 0 37 17 42 887.3 0 42 18 28 887.3 0 28 19 30 887.3 0 30 20 20 887.3 0 20 21 28 887.3 0 28 22 36 887.3 0 36 23 35 887.3 0 35 24 45 887.3 0 45 25 34 887.3 0 34 26 44 887.3 0 44 27 30 887.3 0 30 28 27 887.3 0 27 29 60 887.3 0 60 30 49 887.3 0 49 31 45 887.3 0 45 32 45 887.3 0 45 33 50 887.3 0 50 34 45 887.3 0 45 35 32 887.3 0 32 36 30 887.3 0 30 37 46 887.3 0 46 38 58 887.3 0 58 39 28 887.3 0 28 40 42 887.3 0 42 41 50 887.3 0 50 42 36 887.3 0 36 43 42 887.3 0 42 44 29 887.3 0 29 45 31 887.3 0 31 46 37 887.3 0 37 47 44 887.3 0 44 48 38 887.3 0 38 49 30 887.3 0 30 50 70 887.3 0 70 51 38 887.3 0 38 52 31 887.3 0 31 53 35 887.3 0 35 54 37 887.3 0 37 55 40 887.3 0 40 56 33 887.3 0 33 57 19 887.3 0 19 58 25 887.3 0 25 59 35 887.3 0 35 60 44 887.3 0 44 61 48 887.3 0 48 62 28 887.3 0 28 63 28 887.3 0 28 64 52 887.3 0 52 65 53 887.3 0 53 66 57 887.3 0 57 67 33 887.3 0 33 68 52 887.3 0 52 69 151 887.3 0 151 70 165 887.3 0 165 71 164 887.3 0 164 72 44 887.3 0 44 73 30 887.3 0 30 74 46 887.3 0 46 75 34 887.3 0 34 76 38 887.3 0 38 77 40 887.3 0 40 78 32 887.3 0 32 79 146 887.3 0 146 80 132 887.3 0 132 81 204 887.3 0 204 82 27 887.3 0 27 83 35 887.3 0 35 84 29 887.3 0 29 85 34 887.3 0 34 86 28 887.3 0 28 87 27 887.3 0 27 88 40 887.3 0 40 89 30 887.3 0 30 90 37 887.3 0 37 91 38 887.3 0 38 92 35 887.3 0 35 93 33 887.3 0 33 94 48 887.3 0 48 95 40 887.3 0 40 96 49 887.3 0 49 97 28 887.3 0 28 98 44 887.3 0 44 99 57 887.3 0 57 100 70 887.3 0 70 101 57 887.3 0 57 102 44 887.3 0 44 103 39 887.3 0 39 104 32 887.3 0 32 105 26 887.3 0 26 106 33 887.3 0 33 107 38 887.3 0 38 108 37 887.3 0 37 109 29 887.3 0 29 110 43 887.3 0 43 111 65 887.3 0 65 112 66 887.3 0 66 113 100 887.3 0 100 114 65 887.3 0 65 115 46 887.3 0 46 116 37 887.3 0 37 117 219 887.3 0 219 118 115 887.3 0 115 119 112 887.3 0 112 120 50 887.3 0 50 121 34 887.3 0 34 122 38 887.3 0 38 123 48 887.3 0 48 124 39 887.3 0 39 125 41 887.3 0 41 126 62 887.3 0 62 127 62 887.3 0 62 128 65 887.3 0 65 129 88 887.3 0 88 130 70 887.3 0 70 131 137 887.3 0 137 132 118 887.3 0 118 133 106 887.3 0 106 134 70 887.3 0 70 135 103 887.3 0 103 136 144 887.3 0 144 137 404 887.3 0 404 138 197 887.3 0 197 139 72 887.3 0 72 140 68 887.3 0 68 141 41 887.3 0 41 142 43 887.3 0 43 143 52 887.3 0 52 144 64 887.3 0 64 145 60 887.3 0 60 146 66 887.3 0 66 147 66 887.3 0 66 148 49 887.3 0 49 149 33 887.3 0 33 150 35 887.3 0 35 151 52 887.3 0 52 152 52 887.3 0 52 153 68 887.3 0 68 154 94 887.3 0 94 155 72 887.3 0 72 156 603 887.3 0 603 157 118 887.3 0 118 158 77 887.3 0 77 159 51 887.3 0 51 160 68 887.3 0 68 161 60 887.3 0 60 162 76 887.3 0 76 163 70 887.3 0 70 164 100 887.3 0 100 165 218 887.3 0 218 166 84 887.3 0 84 167 149 887.3 0 149 168 155 887.3 0 155 169 123 887.3 0 123 170 118 887.3 0 118 171 194 887.3 0 194 172 161 887.3 0 161 173 185 887.3 0 185 174 204 887.3 0 204 175 484 887.3 0 484 176 673 887.3 0 673 177 1026 887.3 0 1026 178 8387 887.3 0 8387 179 4903 887.3 0 4903 180 15345 887.3 0 15345 181 4117 887.3 0 4117 182 6060 887.3 0 6060 183 1312 887.3 0 1312 184 156 887.3 0 156 185 103 887.3 0 103 186 51 887.3 0 51 187 57 887.3 0 57 188 157 887.3 0 157 189 84 887.3 0 84 190 52 887.3 0 52 191 77 887.3 0 77 192 121 887.3 0 121 193 79 887.3 0 79 194 148 887.3 0 148 195 188 887.3 0 188 196 698 887.3 0 698 197 878 887.3 0 878 198 581 887.3 0 581 199 148 887.3 0 148 200 166 887.3 0 166 201 302 887.3 0 302 202 612 887.3 0 612 203 351 887.3 0 351 204 221 887.3 0 221 205 29 887.3 0 29 206 44 887.3 0 44 207 49 887.3 0 49 208 38 887.3 0 38 209 48 887.3 0 48 210 40 887.3 0 40 211 47 887.3 0 47 212 40 887.3 0 40 213 55 887.3 0 55 214 55 887.3 0 55 215 100 887.3 0 100 216 127 887.3 0 127 217 198 887.3 0 198 218 52 887.3 0 52 219 60 887.3 0 60 220 55 887.3 0 55 221 89 887.3 0 89 222 254 887.3 0 254 223 428 887.3 0 428 224 305 887.3 0 305 225 271 887.3 0 271 226 57 887.3 0 57 227 83 887.3 0 83 228 56 887.3 0 56 229 53 887.3 0 53 230 49 887.3 0 49 231 67 887.3 0 67 232 82 887.3 0 82 233 58 887.3 0 58 234 399 887.3 0 399 235 319 887.3 0 319 236 572 887.3 0 572 237 180 887.3 0 180 238 205 887.3 0 205 239 1264 887.3 0 1264 240 428 887.3 0 428 241 571 887.3 0 571 242 310 887.3 0 310 243 422 887.3 0 422 244 162 887.3 0 162 245 80 887.3 0 80 246 83 887.3 0 83 247 49 887.3 0 49 248 59 887.3 0 59 249 48 887.3 0 48 250 91 887.3 0 91 251 92 887.3 0 92 252 111 887.3 0 111 253 167 887.3 0 167 254 174 887.3 0 174 255 78 887.3 0 78 256 225 887.3 0 225 257 239 887.3 0 239 258 291 887.3 0 291 259 888 887.3 0 888 260 942 887.3 0 942 261 567 887.3 0 567 262 176 887.3 0 176 263 232 887.3 0 232 264 1563 887.3 0 1563 265 4106 887.3 0 4106 266 3468 887.3 0 3468 267 1154 887.3 0 1154 268 47 887.3 0 47 269 57 887.3 0 57 270 49 887.3 0 49 271 62 887.3 0 62 272 50 887.3 0 50 273 66 887.3 0 66 274 85 887.3 0 85 275 66 887.3 0 66 276 139 887.3 0 139 277 99 887.3 0 99 278 132 887.3 0 132 279 69 887.3 0 69 280 56 887.3 0 56 281 31 887.3 0 31 282 60 887.3 0 60 283 49 887.3 0 49 284 60 887.3 0 60 285 67 887.3 0 67 286 57 887.3 0 57 287 68 887.3 0 68 288 81 887.3 0 81 289 87 887.3 0 87 290 167 887.3 0 167 291 2703 887.3 0 2703 292 1657 887.3 0 1657 293 1745 887.3 0 1745 294 349 887.3 0 349 295 230 887.3 0 230 296 109 887.3 0 109 297 177 887.3 0 177 298 90 887.3 0 90 299 203 887.3 0 203 300 135 887.3 0 135 301 111 887.3 0 111 Finished in 15.73 s (75 us/read; 0.80 M reads/minute). === Summary === Total reads processed: 210,585 Reads with adapters: 83,490 (39.6%) Reads that were too short: 25 (0.0%) Reads written (passing filters): 83,465 (39.6%) Total basepairs processed: 52,457,838 bp Total written (filtered): 22,617,671 bp (43.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 83490 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 0 3 18 32 0.0 3 0 0 32 19 580 0.0 3 0 562 13 2 3 20 81755 0.0 4 80384 1323 42 4 2 21 191 0.0 4 32 157 2 22 8 0.0 4 1 0 7 23 48 0.0 4 3 0 1 42 2 34 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 40 1 0.0 4 1 53 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 83 1 0.0 4 1 125 1 0.0 4 0 0 0 0 1 126 7 0.0 4 0 0 0 0 7 127 736 0.0 4 0 0 0 0 736 128 3 0.0 4 0 0 0 0 3 129 2 0.0 4 0 0 0 0 2 130 94 0.0 4 0 0 0 0 94 137 1 0.0 4 0 0 0 0 1 152 2 0.0 4 0 0 0 0 2 196 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 0 5 229 4 0.0 4 0 0 0 0 4 232 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 273 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 286 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.93 s (59 us/read; 1.02 M reads/minute). === Summary === Total reads processed: 83,465 Reads with adapters: 9,849 (11.8%) Reads written (passing filters): 83,465 (100.0%) Total basepairs processed: 22,617,671 bp Total written (filtered): 22,522,784 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 9849 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.9% C: 2.5% G: 95.3% T: 1.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 60 1304.1 0 60 4 19 326.0 0 19 5 2 81.5 0 2 6 1 20.4 0 1 7 2 5.1 0 2 8 4 1.3 0 4 9 4578 0.3 0 123 4455 10 4617 0.1 1 128 4489 11 545 0.0 1 4 541 12 10 0.0 1 0 10 13 5 0.0 1 0 5 150 5 0.0 1 0 5 267 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.