This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTGCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 174.85 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 268,957 (7.4%) Reads that were too short: 197,991 (5.4%) Reads written (passing filters): 70,966 (2.0%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 15,519,314 bp (1.4%) === Adapter 1 === Sequence: CGTGCG; Type: regular 5'; Length: 6; Trimmed: 268957 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2607 887.3 0 2607 7 2424 887.3 0 2424 8 2097 887.3 0 2097 9 68 887.3 0 68 10 144 887.3 0 144 11 27 887.3 0 27 12 39 887.3 0 39 13 38 887.3 0 38 14 45 887.3 0 45 15 30 887.3 0 30 16 31 887.3 0 31 17 61 887.3 0 61 18 27 887.3 0 27 19 28 887.3 0 28 20 42 887.3 0 42 21 56 887.3 0 56 22 23 887.3 0 23 23 24 887.3 0 24 24 35 887.3 0 35 25 35 887.3 0 35 26 20 887.3 0 20 27 22 887.3 0 22 28 25 887.3 0 25 29 36 887.3 0 36 30 48 887.3 0 48 31 26 887.3 0 26 32 32 887.3 0 32 33 24 887.3 0 24 34 22 887.3 0 22 35 34 887.3 0 34 36 28 887.3 0 28 37 35 887.3 0 35 38 22 887.3 0 22 39 31 887.3 0 31 40 35 887.3 0 35 41 26 887.3 0 26 42 29 887.3 0 29 43 34 887.3 0 34 44 36 887.3 0 36 45 22 887.3 0 22 46 35 887.3 0 35 47 73 887.3 0 73 48 72 887.3 0 72 49 53 887.3 0 53 50 50 887.3 0 50 51 109 887.3 0 109 52 63 887.3 0 63 53 29 887.3 0 29 54 44 887.3 0 44 55 81 887.3 0 81 56 59 887.3 0 59 57 38 887.3 0 38 58 50 887.3 0 50 59 68 887.3 0 68 60 62 887.3 0 62 61 76 887.3 0 76 62 209 887.3 0 209 63 94 887.3 0 94 64 113 887.3 0 113 65 146 887.3 0 146 66 81 887.3 0 81 67 65 887.3 0 65 68 143 887.3 0 143 69 196 887.3 0 196 70 269 887.3 0 269 71 251 887.3 0 251 72 394 887.3 0 394 73 637 887.3 0 637 74 1258 887.3 0 1258 75 22480 887.3 0 22480 76 13737 887.3 0 13737 77 9413 887.3 0 9413 78 58 887.3 0 58 79 38 887.3 0 38 80 50 887.3 0 50 81 33 887.3 0 33 82 34 887.3 0 34 83 32 887.3 0 32 84 35 887.3 0 35 85 45 887.3 0 45 86 39 887.3 0 39 87 42 887.3 0 42 88 89 887.3 0 89 89 43 887.3 0 43 90 86 887.3 0 86 91 70 887.3 0 70 92 61 887.3 0 61 93 23 887.3 0 23 94 21 887.3 0 21 95 68 887.3 0 68 96 30 887.3 0 30 97 15 887.3 0 15 98 30 887.3 0 30 99 21 887.3 0 21 100 13 887.3 0 13 101 28 887.3 0 28 102 35 887.3 0 35 103 25 887.3 0 25 104 33 887.3 0 33 105 35 887.3 0 35 106 33 887.3 0 33 107 28 887.3 0 28 108 23 887.3 0 23 109 41 887.3 0 41 110 27 887.3 0 27 111 19 887.3 0 19 112 26 887.3 0 26 113 26 887.3 0 26 114 19 887.3 0 19 115 38 887.3 0 38 116 32 887.3 0 32 117 43 887.3 0 43 118 35 887.3 0 35 119 63 887.3 0 63 120 68 887.3 0 68 121 100 887.3 0 100 122 35 887.3 0 35 123 40 887.3 0 40 124 37 887.3 0 37 125 116 887.3 0 116 126 166 887.3 0 166 127 176 887.3 0 176 128 91 887.3 0 91 129 58 887.3 0 58 130 75 887.3 0 75 131 51 887.3 0 51 132 49 887.3 0 49 133 53 887.3 0 53 134 80 887.3 0 80 135 164 887.3 0 164 136 142 887.3 0 142 137 111 887.3 0 111 138 66 887.3 0 66 139 124 887.3 0 124 140 57 887.3 0 57 141 40 887.3 0 40 142 90 887.3 0 90 143 208 887.3 0 208 144 102 887.3 0 102 145 59 887.3 0 59 146 35 887.3 0 35 147 32 887.3 0 32 148 43 887.3 0 43 149 32 887.3 0 32 150 38 887.3 0 38 151 39 887.3 0 39 152 27 887.3 0 27 153 21 887.3 0 21 154 27 887.3 0 27 155 39 887.3 0 39 156 44 887.3 0 44 157 43 887.3 0 43 158 37 887.3 0 37 159 28 887.3 0 28 160 33 887.3 0 33 161 93 887.3 0 93 162 75 887.3 0 75 163 113 887.3 0 113 164 49 887.3 0 49 165 77 887.3 0 77 166 163 887.3 0 163 167 196 887.3 0 196 168 203 887.3 0 203 169 82 887.3 0 82 170 28 887.3 0 28 171 36 887.3 0 36 172 34 887.3 0 34 173 32 887.3 0 32 174 42 887.3 0 42 175 17 887.3 0 17 176 32 887.3 0 32 177 47 887.3 0 47 178 50 887.3 0 50 179 47 887.3 0 47 180 74 887.3 0 74 181 102 887.3 0 102 182 105 887.3 0 105 183 119 887.3 0 119 184 88 887.3 0 88 185 73 887.3 0 73 186 242 887.3 0 242 187 231 887.3 0 231 188 289 887.3 0 289 189 147 887.3 0 147 190 91 887.3 0 91 191 77 887.3 0 77 192 127 887.3 0 127 193 629 887.3 0 629 194 449 887.3 0 449 195 357 887.3 0 357 196 181 887.3 0 181 197 1208 887.3 0 1208 198 826 887.3 0 826 199 534 887.3 0 534 200 91 887.3 0 91 201 133 887.3 0 133 202 72 887.3 0 72 203 131 887.3 0 131 204 226 887.3 0 226 205 345 887.3 0 345 206 70 887.3 0 70 207 58 887.3 0 58 208 88 887.3 0 88 209 92 887.3 0 92 210 126 887.3 0 126 211 191 887.3 0 191 212 100 887.3 0 100 213 170 887.3 0 170 214 207 887.3 0 207 215 213 887.3 0 213 216 160 887.3 0 160 217 421 887.3 0 421 218 532 887.3 0 532 219 439 887.3 0 439 220 268 887.3 0 268 221 189 887.3 0 189 222 126 887.3 0 126 223 552 887.3 0 552 224 549 887.3 0 549 225 660 887.3 0 660 226 220 887.3 0 220 227 278 887.3 0 278 228 230 887.3 0 230 229 248 887.3 0 248 230 359 887.3 0 359 231 197 887.3 0 197 232 226 887.3 0 226 233 375 887.3 0 375 234 482 887.3 0 482 235 830 887.3 0 830 236 1178 887.3 0 1178 237 1136 887.3 0 1136 238 13596 887.3 0 13596 239 24994 887.3 0 24994 240 20443 887.3 0 20443 241 384 887.3 0 384 242 376 887.3 0 376 243 468 887.3 0 468 244 472 887.3 0 472 245 670 887.3 0 670 246 996 887.3 0 996 247 1636 887.3 0 1636 248 20457 887.3 0 20457 249 36935 887.3 0 36935 250 21724 887.3 0 21724 251 15142 887.3 0 15142 252 285 887.3 0 285 253 175 887.3 0 175 254 110 887.3 0 110 255 166 887.3 0 166 256 182 887.3 0 182 257 112 887.3 0 112 258 83 887.3 0 83 259 282 887.3 0 282 260 149 887.3 0 149 261 223 887.3 0 223 262 365 887.3 0 365 263 193 887.3 0 193 264 78 887.3 0 78 265 82 887.3 0 82 266 81 887.3 0 81 267 72 887.3 0 72 268 93 887.3 0 93 269 187 887.3 0 187 270 133 887.3 0 133 271 209 887.3 0 209 272 177 887.3 0 177 273 141 887.3 0 141 274 101 887.3 0 101 275 96 887.3 0 96 276 90 887.3 0 90 277 135 887.3 0 135 278 272 887.3 0 272 279 381 887.3 0 381 280 568 887.3 0 568 281 554 887.3 0 554 282 5578 887.3 0 5578 283 4132 887.3 0 4132 284 5177 887.3 0 5177 285 422 887.3 0 422 286 354 887.3 0 354 287 386 887.3 0 386 288 194 887.3 0 194 289 156 887.3 0 156 290 143 887.3 0 143 291 177 887.3 0 177 292 217 887.3 0 217 293 192 887.3 0 192 294 503 887.3 0 503 295 1190 887.3 0 1190 296 1009 887.3 0 1009 297 612 887.3 0 612 298 389 887.3 0 389 299 470 887.3 0 470 300 453 887.3 0 453 301 425 887.3 0 425 Finished in 4.40 s (62 us/read; 0.97 M reads/minute). === Summary === Total reads processed: 70,966 Reads with adapters: 3,932 (5.5%) Reads that were too short: 250 (0.4%) Reads written (passing filters): 3,682 (5.2%) Total basepairs processed: 15,519,314 bp Total written (filtered): 806,950 bp (5.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3932 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 22 0.0 3 0 20 1 1 17 2254 0.0 3 2151 84 15 4 18 7 0.0 3 0 5 2 19 5 0.0 3 0 0 3 2 20 1 0.0 3 0 0 0 1 22 1 0.0 3 0 0 0 1 25 7 0.0 3 0 0 0 7 27 3 0.0 3 0 0 0 3 29 4 0.0 3 0 0 0 4 31 4 0.0 3 0 0 0 4 34 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 45 3 0.0 3 0 0 0 3 46 1 0.0 3 0 0 1 47 13 0.0 3 0 0 0 13 48 7 0.0 3 0 0 0 7 49 2 0.0 3 0 0 0 2 50 1 0.0 3 0 0 0 1 51 3 0.0 3 0 0 0 3 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 56 3 0.0 3 0 0 0 3 59 3 0.0 3 0 0 0 3 60 4 0.0 3 0 0 0 4 61 5 0.0 3 0 0 0 5 62 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 0 2 64 3 0.0 3 0 0 0 3 65 5 0.0 3 0 0 1 4 66 5 0.0 3 0 0 0 5 67 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 73 3 0.0 3 0 0 0 3 74 3 0.0 3 0 0 0 3 77 1 0.0 3 0 0 0 1 78 2 0.0 3 0 0 0 2 79 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 86 3 0.0 3 0 0 0 3 89 1 0.0 3 0 0 0 1 90 3 0.0 3 0 0 0 3 91 71 0.0 3 0 0 2 69 92 18 0.0 3 0 0 0 18 93 8 0.0 3 0 0 0 8 94 13 0.0 3 0 0 0 13 95 3 0.0 3 0 0 0 3 96 202 0.0 3 0 0 5 197 97 6 0.0 3 0 0 0 6 98 464 0.0 3 0 0 4 460 99 112 0.0 3 0 0 2 110 100 1 0.0 3 0 0 0 1 101 35 0.0 3 0 0 0 35 102 7 0.0 3 0 0 0 7 103 1 0.0 3 0 0 0 1 104 2 0.0 3 0 0 0 2 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 8 0.0 3 0 0 1 7 109 7 0.0 3 0 0 0 7 110 3 0.0 3 0 0 0 3 111 5 0.0 3 0 0 0 5 112 2 0.0 3 0 0 0 2 113 3 0.0 3 0 0 1 2 114 2 0.0 3 0 0 0 2 115 29 0.0 3 0 0 1 28 116 158 0.0 3 0 0 3 155 117 98 0.0 3 0 0 0 98 118 11 0.0 3 0 0 0 11 119 19 0.0 3 0 0 0 19 120 21 0.0 3 0 0 1 20 121 3 0.0 3 0 0 0 3 122 13 0.0 3 0 0 0 13 123 1 0.0 3 0 0 0 1 124 8 0.0 3 0 0 0 8 125 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 132 2 0.0 3 0 0 0 2 135 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 140 2 0.0 3 0 0 0 2 141 3 0.0 3 0 0 0 3 142 8 0.0 3 0 0 0 8 145 1 0.0 3 0 0 0 1 147 4 0.0 3 0 0 0 4 148 1 0.0 3 0 0 0 1 149 6 0.0 3 0 0 0 6 150 3 0.0 3 0 0 0 3 151 14 0.0 3 0 0 1 13 152 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 166 2 0.0 3 0 0 0 2 167 12 0.0 3 0 0 0 12 168 6 0.0 3 0 0 0 6 171 1 0.0 3 0 0 0 1 176 15 0.0 3 0 0 0 15 177 3 0.0 3 0 0 0 3 179 1 0.0 3 0 0 0 1 180 2 0.0 3 0 0 0 2 181 1 0.0 3 0 0 0 1 184 2 0.0 3 0 0 0 2 185 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 188 2 0.0 3 0 0 0 2 189 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 203 12 0.0 3 0 0 0 12 204 1 0.0 3 0 0 0 1 206 5 0.0 3 0 0 0 5 207 4 0.0 3 0 0 0 4 208 5 0.0 3 0 0 0 5 209 3 0.0 3 0 0 0 3 211 4 0.0 3 0 0 0 4 212 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 215 2 0.0 3 0 0 0 2 216 2 0.0 3 0 0 0 2 217 6 0.0 3 0 0 1 5 218 9 0.0 3 0 0 0 9 219 8 0.0 3 0 0 0 8 220 2 0.0 3 0 0 0 2 221 2 0.0 3 0 0 0 2 222 1 0.0 3 0 0 0 1 225 2 0.0 3 0 0 0 2 226 12 0.0 3 0 0 0 12 228 3 0.0 3 0 0 0 3 230 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.20 s (54 us/read; 1.10 M reads/minute). === Summary === Total reads processed: 3,682 Reads with adapters: 25 (0.7%) Reads written (passing filters): 3,682 (100.0%) Total basepairs processed: 806,950 bp Total written (filtered): 806,874 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 25 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 20.0% C: 64.0% G: 16.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 24 57.5 0 24 4 1 14.4 0 1