This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTGCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 176.51 s (49 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 272,640 (7.5%) Reads that were too short: 216,670 (6.0%) Reads written (passing filters): 55,970 (1.5%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 12,647,733 bp (1.2%) === Adapter 1 === Sequence: CGTGCG; Type: regular 5'; Length: 6; Trimmed: 272640 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3326 887.3 0 3326 7 2516 887.3 0 2516 8 2187 887.3 0 2187 9 33 887.3 0 33 10 45 887.3 0 45 11 29 887.3 0 29 12 32 887.3 0 32 13 33 887.3 0 33 14 29 887.3 0 29 15 34 887.3 0 34 16 35 887.3 0 35 17 35 887.3 0 35 18 19 887.3 0 19 19 30 887.3 0 30 20 44 887.3 0 44 21 40 887.3 0 40 22 22 887.3 0 22 23 24 887.3 0 24 24 32 887.3 0 32 25 24 887.3 0 24 26 17 887.3 0 17 27 18 887.3 0 18 28 25 887.3 0 25 29 27 887.3 0 27 30 20 887.3 0 20 31 12 887.3 0 12 32 19 887.3 0 19 33 16 887.3 0 16 34 15 887.3 0 15 35 34 887.3 0 34 36 23 887.3 0 23 37 26 887.3 0 26 38 25 887.3 0 25 39 17 887.3 0 17 40 24 887.3 0 24 41 19 887.3 0 19 42 48 887.3 0 48 43 29 887.3 0 29 44 32 887.3 0 32 45 20 887.3 0 20 46 23 887.3 0 23 47 22 887.3 0 22 48 25 887.3 0 25 49 34 887.3 0 34 50 56 887.3 0 56 51 71 887.3 0 71 52 46 887.3 0 46 53 33 887.3 0 33 54 51 887.3 0 51 55 66 887.3 0 66 56 72 887.3 0 72 57 39 887.3 0 39 58 57 887.3 0 57 59 54 887.3 0 54 60 46 887.3 0 46 61 52 887.3 0 52 62 150 887.3 0 150 63 65 887.3 0 65 64 74 887.3 0 74 65 120 887.3 0 120 66 68 887.3 0 68 67 61 887.3 0 61 68 131 887.3 0 131 69 140 887.3 0 140 70 167 887.3 0 167 71 150 887.3 0 150 72 249 887.3 0 249 73 311 887.3 0 311 74 593 887.3 0 593 75 18163 887.3 0 18163 76 10269 887.3 0 10269 77 8196 887.3 0 8196 78 69 887.3 0 69 79 27 887.3 0 27 80 30 887.3 0 30 81 32 887.3 0 32 82 29 887.3 0 29 83 21 887.3 0 21 84 25 887.3 0 25 85 18 887.3 0 18 86 20 887.3 0 20 87 30 887.3 0 30 88 40 887.3 0 40 89 23 887.3 0 23 90 24 887.3 0 24 91 58 887.3 0 58 92 26 887.3 0 26 93 17 887.3 0 17 94 29 887.3 0 29 95 66 887.3 0 66 96 44 887.3 0 44 97 29 887.3 0 29 98 53 887.3 0 53 99 34 887.3 0 34 100 17 887.3 0 17 101 29 887.3 0 29 102 23 887.3 0 23 103 30 887.3 0 30 104 22 887.3 0 22 105 43 887.3 0 43 106 31 887.3 0 31 107 39 887.3 0 39 108 31 887.3 0 31 109 34 887.3 0 34 110 25 887.3 0 25 111 20 887.3 0 20 112 16 887.3 0 16 113 22 887.3 0 22 114 17 887.3 0 17 115 43 887.3 0 43 116 23 887.3 0 23 117 31 887.3 0 31 118 27 887.3 0 27 119 45 887.3 0 45 120 42 887.3 0 42 121 69 887.3 0 69 122 30 887.3 0 30 123 41 887.3 0 41 124 34 887.3 0 34 125 92 887.3 0 92 126 171 887.3 0 171 127 159 887.3 0 159 128 56 887.3 0 56 129 40 887.3 0 40 130 27 887.3 0 27 131 28 887.3 0 28 132 31 887.3 0 31 133 26 887.3 0 26 134 59 887.3 0 59 135 70 887.3 0 70 136 60 887.3 0 60 137 61 887.3 0 61 138 43 887.3 0 43 139 104 887.3 0 104 140 51 887.3 0 51 141 25 887.3 0 25 142 43 887.3 0 43 143 133 887.3 0 133 144 71 887.3 0 71 145 44 887.3 0 44 146 40 887.3 0 40 147 31 887.3 0 31 148 22 887.3 0 22 149 25 887.3 0 25 150 46 887.3 0 46 151 26 887.3 0 26 152 25 887.3 0 25 153 30 887.3 0 30 154 36 887.3 0 36 155 28 887.3 0 28 156 34 887.3 0 34 157 38 887.3 0 38 158 48 887.3 0 48 159 27 887.3 0 27 160 27 887.3 0 27 161 48 887.3 0 48 162 32 887.3 0 32 163 37 887.3 0 37 164 54 887.3 0 54 165 56 887.3 0 56 166 66 887.3 0 66 167 69 887.3 0 69 168 93 887.3 0 93 169 56 887.3 0 56 170 29 887.3 0 29 171 29 887.3 0 29 172 39 887.3 0 39 173 37 887.3 0 37 174 34 887.3 0 34 175 32 887.3 0 32 176 26 887.3 0 26 177 34 887.3 0 34 178 44 887.3 0 44 179 40 887.3 0 40 180 66 887.3 0 66 181 95 887.3 0 95 182 80 887.3 0 80 183 79 887.3 0 79 184 46 887.3 0 46 185 51 887.3 0 51 186 94 887.3 0 94 187 130 887.3 0 130 188 126 887.3 0 126 189 87 887.3 0 87 190 56 887.3 0 56 191 72 887.3 0 72 192 75 887.3 0 75 193 178 887.3 0 178 194 174 887.3 0 174 195 96 887.3 0 96 196 77 887.3 0 77 197 591 887.3 0 591 198 485 887.3 0 485 199 219 887.3 0 219 200 46 887.3 0 46 201 98 887.3 0 98 202 55 887.3 0 55 203 75 887.3 0 75 204 95 887.3 0 95 205 157 887.3 0 157 206 80 887.3 0 80 207 57 887.3 0 57 208 70 887.3 0 70 209 40 887.3 0 40 210 64 887.3 0 64 211 166 887.3 0 166 212 68 887.3 0 68 213 74 887.3 0 74 214 77 887.3 0 77 215 78 887.3 0 78 216 81 887.3 0 81 217 245 887.3 0 245 218 327 887.3 0 327 219 268 887.3 0 268 220 177 887.3 0 177 221 201 887.3 0 201 222 80 887.3 0 80 223 420 887.3 0 420 224 467 887.3 0 467 225 523 887.3 0 523 226 176 887.3 0 176 227 182 887.3 0 182 228 210 887.3 0 210 229 175 887.3 0 175 230 316 887.3 0 316 231 159 887.3 0 159 232 212 887.3 0 212 233 326 887.3 0 326 234 431 887.3 0 431 235 820 887.3 0 820 236 1148 887.3 0 1148 237 1161 887.3 0 1161 238 17041 887.3 0 17041 239 32255 887.3 0 32255 240 25711 887.3 0 25711 241 397 887.3 0 397 242 402 887.3 0 402 243 426 887.3 0 426 244 418 887.3 0 418 245 611 887.3 0 611 246 831 887.3 0 831 247 1448 887.3 0 1448 248 21431 887.3 0 21431 249 41383 887.3 0 41383 250 24783 887.3 0 24783 251 17953 887.3 0 17953 252 103 887.3 0 103 253 42 887.3 0 42 254 28 887.3 0 28 255 39 887.3 0 39 256 35 887.3 0 35 257 53 887.3 0 53 258 61 887.3 0 61 259 281 887.3 0 281 260 103 887.3 0 103 261 104 887.3 0 104 262 239 887.3 0 239 263 99 887.3 0 99 264 55 887.3 0 55 265 31 887.3 0 31 266 36 887.3 0 36 267 39 887.3 0 39 268 62 887.3 0 62 269 119 887.3 0 119 270 74 887.3 0 74 271 112 887.3 0 112 272 106 887.3 0 106 273 80 887.3 0 80 274 50 887.3 0 50 275 66 887.3 0 66 276 67 887.3 0 67 277 74 887.3 0 74 278 146 887.3 0 146 279 273 887.3 0 273 280 327 887.3 0 327 281 374 887.3 0 374 282 5001 887.3 0 5001 283 3796 887.3 0 3796 284 4863 887.3 0 4863 285 202 887.3 0 202 286 167 887.3 0 167 287 120 887.3 0 120 288 83 887.3 0 83 289 58 887.3 0 58 290 151 887.3 0 151 291 85 887.3 0 85 292 107 887.3 0 107 293 87 887.3 0 87 294 561 887.3 0 561 295 1391 887.3 0 1391 296 942 887.3 0 942 297 297 887.3 0 297 298 248 887.3 0 248 299 246 887.3 0 246 300 343 887.3 0 343 301 293 887.3 0 293 Finished in 3.91 s (70 us/read; 0.86 M reads/minute). === Summary === Total reads processed: 55,970 Reads with adapters: 5,703 (10.2%) Reads that were too short: 10 (0.0%) Reads written (passing filters): 5,693 (10.2%) Total basepairs processed: 12,647,733 bp Total written (filtered): 1,544,487 bp (12.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 5703 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 2 19 37 0.0 3 0 35 1 1 20 2340 0.0 4 2299 39 2 21 56 0.0 4 7 49 22 3151 0.0 4 3093 56 2 23 8 0.0 4 1 5 0 2 24 2 0.0 4 0 0 2 25 3 0.0 4 0 0 0 3 59 91 0.0 4 0 0 0 0 91 62 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 161 2 0.0 4 0 0 0 0 2 162 1 0.0 4 0 0 0 0 1 192 1 0.0 4 0 0 0 0 1 246 1 0.0 4 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.32 s (56 us/read; 1.07 M reads/minute). === Summary === Total reads processed: 5,693 Reads with adapters: 422 (7.4%) Reads written (passing filters): 5,693 (100.0%) Total basepairs processed: 1,544,487 bp Total written (filtered): 1,539,363 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 422 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.5% C: 3.3% G: 94.5% T: 1.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 89.0 0 1 4 2 22.2 0 2 5 1 5.6 0 1 9 3 0.0 0 0 3 10 55 0.0 1 1 54 11 353 0.0 1 2 351 12 3 0.0 1 0 3 13 1 0.0 1 0 1 151 1 0.0 1 0 1 224 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.