This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 173.58 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 196,858 (5.4%) Reads that were too short: 49,536 (1.4%) Reads written (passing filters): 147,322 (4.1%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 30,987,135 bp (2.8%) === Adapter 1 === Sequence: AAGACA; Type: regular 5'; Length: 6; Trimmed: 196858 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1267 887.3 0 1267 7 31235 887.3 0 31235 8 684 887.3 0 684 9 30787 887.3 0 30787 10 69 887.3 0 69 11 26 887.3 0 26 12 22 887.3 0 22 13 14 887.3 0 14 14 18 887.3 0 18 15 17 887.3 0 17 16 19 887.3 0 19 17 23 887.3 0 23 18 17 887.3 0 17 19 16 887.3 0 16 20 43 887.3 0 43 21 18 887.3 0 18 22 27 887.3 0 27 23 18 887.3 0 18 24 16 887.3 0 16 25 16 887.3 0 16 26 26 887.3 0 26 27 14 887.3 0 14 28 13 887.3 0 13 29 42 887.3 0 42 30 15 887.3 0 15 31 21 887.3 0 21 32 20 887.3 0 20 33 18 887.3 0 18 34 18 887.3 0 18 35 19 887.3 0 19 36 39 887.3 0 39 37 26 887.3 0 26 38 22 887.3 0 22 39 17 887.3 0 17 40 17 887.3 0 17 41 25 887.3 0 25 42 52 887.3 0 52 43 13 887.3 0 13 44 15 887.3 0 15 45 17 887.3 0 17 46 25 887.3 0 25 47 16 887.3 0 16 48 24 887.3 0 24 49 17 887.3 0 17 50 21 887.3 0 21 51 20 887.3 0 20 52 28 887.3 0 28 53 21 887.3 0 21 54 66 887.3 0 66 55 56 887.3 0 56 56 70 887.3 0 70 57 30 887.3 0 30 58 93 887.3 0 93 59 63 887.3 0 63 60 19 887.3 0 19 61 20 887.3 0 20 62 30 887.3 0 30 63 38 887.3 0 38 64 124 887.3 0 124 65 162 887.3 0 162 66 110 887.3 0 110 67 43 887.3 0 43 68 45 887.3 0 45 69 57 887.3 0 57 70 68 887.3 0 68 71 329 887.3 0 329 72 140 887.3 0 140 73 421 887.3 0 421 74 382 887.3 0 382 75 312 887.3 0 312 76 279 887.3 0 279 77 63 887.3 0 63 78 186 887.3 0 186 79 306 887.3 0 306 80 249 887.3 0 249 81 295 887.3 0 295 82 148 887.3 0 148 83 858 887.3 0 858 84 307 887.3 0 307 85 265 887.3 0 265 86 51 887.3 0 51 87 66 887.3 0 66 88 127 887.3 0 127 89 34 887.3 0 34 90 42 887.3 0 42 91 105 887.3 0 105 92 51 887.3 0 51 93 34 887.3 0 34 94 69 887.3 0 69 95 57 887.3 0 57 96 88 887.3 0 88 97 74 887.3 0 74 98 61 887.3 0 61 99 50 887.3 0 50 100 90 887.3 0 90 101 183 887.3 0 183 102 144 887.3 0 144 103 165 887.3 0 165 104 289 887.3 0 289 105 289 887.3 0 289 106 258 887.3 0 258 107 1454 887.3 0 1454 108 2533 887.3 0 2533 109 2311 887.3 0 2311 110 231 887.3 0 231 111 242 887.3 0 242 112 276 887.3 0 276 113 304 887.3 0 304 114 258 887.3 0 258 115 250 887.3 0 250 116 381 887.3 0 381 117 352 887.3 0 352 118 284 887.3 0 284 119 345 887.3 0 345 120 1399 887.3 0 1399 121 1054 887.3 0 1054 122 876 887.3 0 876 123 410 887.3 0 410 124 374 887.3 0 374 125 386 887.3 0 386 126 296 887.3 0 296 127 201 887.3 0 201 128 186 887.3 0 186 129 185 887.3 0 185 130 238 887.3 0 238 131 244 887.3 0 244 132 201 887.3 0 201 133 184 887.3 0 184 134 168 887.3 0 168 135 166 887.3 0 166 136 170 887.3 0 170 137 245 887.3 0 245 138 228 887.3 0 228 139 255 887.3 0 255 140 197 887.3 0 197 141 299 887.3 0 299 142 259 887.3 0 259 143 293 887.3 0 293 144 186 887.3 0 186 145 269 887.3 0 269 146 268 887.3 0 268 147 362 887.3 0 362 148 280 887.3 0 280 149 282 887.3 0 282 150 219 887.3 0 219 151 240 887.3 0 240 152 233 887.3 0 233 153 169 887.3 0 169 154 127 887.3 0 127 155 181 887.3 0 181 156 173 887.3 0 173 157 227 887.3 0 227 158 152 887.3 0 152 159 197 887.3 0 197 160 211 887.3 0 211 161 200 887.3 0 200 162 206 887.3 0 206 163 221 887.3 0 221 164 303 887.3 0 303 165 311 887.3 0 311 166 2142 887.3 0 2142 167 2971 887.3 0 2971 168 2218 887.3 0 2218 169 308 887.3 0 308 170 1448 887.3 0 1448 171 1605 887.3 0 1605 172 992 887.3 0 992 173 284 887.3 0 284 174 260 887.3 0 260 175 210 887.3 0 210 176 254 887.3 0 254 177 311 887.3 0 311 178 496 887.3 0 496 179 403 887.3 0 403 180 941 887.3 0 941 181 7951 887.3 0 7951 182 8068 887.3 0 8068 183 12593 887.3 0 12593 184 2295 887.3 0 2295 185 2057 887.3 0 2057 186 826 887.3 0 826 187 176 887.3 0 176 188 179 887.3 0 179 189 142 887.3 0 142 190 122 887.3 0 122 191 226 887.3 0 226 192 159 887.3 0 159 193 145 887.3 0 145 194 129 887.3 0 129 195 135 887.3 0 135 196 177 887.3 0 177 197 150 887.3 0 150 198 262 887.3 0 262 199 351 887.3 0 351 200 389 887.3 0 389 201 261 887.3 0 261 202 193 887.3 0 193 203 279 887.3 0 279 204 459 887.3 0 459 205 600 887.3 0 600 206 476 887.3 0 476 207 342 887.3 0 342 208 173 887.3 0 173 209 157 887.3 0 157 210 212 887.3 0 212 211 271 887.3 0 271 212 401 887.3 0 401 213 3154 887.3 0 3154 214 3976 887.3 0 3976 215 4581 887.3 0 4581 216 481 887.3 0 481 217 188 887.3 0 188 218 198 887.3 0 198 219 256 887.3 0 256 220 265 887.3 0 265 221 167 887.3 0 167 222 139 887.3 0 139 223 170 887.3 0 170 224 201 887.3 0 201 225 473 887.3 0 473 226 619 887.3 0 619 227 593 887.3 0 593 228 1041 887.3 0 1041 229 794 887.3 0 794 230 540 887.3 0 540 231 136 887.3 0 136 232 137 887.3 0 137 233 133 887.3 0 133 234 144 887.3 0 144 235 119 887.3 0 119 236 126 887.3 0 126 237 201 887.3 0 201 238 166 887.3 0 166 239 219 887.3 0 219 240 284 887.3 0 284 241 269 887.3 0 269 242 581 887.3 0 581 243 352 887.3 0 352 244 307 887.3 0 307 245 272 887.3 0 272 246 380 887.3 0 380 247 356 887.3 0 356 248 263 887.3 0 263 249 222 887.3 0 222 250 151 887.3 0 151 251 174 887.3 0 174 252 176 887.3 0 176 253 152 887.3 0 152 254 258 887.3 0 258 255 346 887.3 0 346 256 244 887.3 0 244 257 257 887.3 0 257 258 167 887.3 0 167 259 176 887.3 0 176 260 310 887.3 0 310 261 341 887.3 0 341 262 507 887.3 0 507 263 391 887.3 0 391 264 416 887.3 0 416 265 338 887.3 0 338 266 534 887.3 0 534 267 6314 887.3 0 6314 268 2920 887.3 0 2920 269 1636 887.3 0 1636 270 307 887.3 0 307 271 141 887.3 0 141 272 125 887.3 0 125 273 203 887.3 0 203 274 175 887.3 0 175 275 207 887.3 0 207 276 284 887.3 0 284 277 223 887.3 0 223 278 209 887.3 0 209 279 269 887.3 0 269 280 762 887.3 0 762 281 727 887.3 0 727 282 357 887.3 0 357 283 224 887.3 0 224 284 125 887.3 0 125 285 126 887.3 0 126 286 177 887.3 0 177 287 165 887.3 0 165 288 148 887.3 0 148 289 148 887.3 0 148 290 141 887.3 0 141 291 174 887.3 0 174 292 150 887.3 0 150 293 183 887.3 0 183 294 171 887.3 0 171 295 179 887.3 0 179 296 149 887.3 0 149 297 131 887.3 0 131 298 193 887.3 0 193 299 352 887.3 0 352 300 323 887.3 0 323 301 414 887.3 0 414 Finished in 9.43 s (64 us/read; 0.94 M reads/minute). === Summary === Total reads processed: 147,322 Reads with adapters: 32,084 (21.8%) Reads that were too short: 159 (0.1%) Reads written (passing filters): 31,925 (21.7%) Total basepairs processed: 30,987,135 bp Total written (filtered): 8,731,623 bp (28.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 32084 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 17 0.0 3 0 0 12 5 16 199 0.0 3 0 168 17 14 17 31176 0.0 3 29563 1368 203 42 18 88 0.0 3 11 64 5 8 19 43 0.0 3 0 1 24 18 20 90 0.0 3 10 4 2 74 21 115 0.0 3 0 0 0 115 22 2 0.0 3 1 0 1 24 28 0.0 3 0 0 1 27 25 15 0.0 3 1 0 0 14 26 1 0.0 3 0 0 0 1 27 6 0.0 3 0 0 0 6 29 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 35 2 0.0 3 0 0 0 2 38 3 0.0 3 0 0 0 3 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 3 0.0 3 0 0 0 3 43 1 0.0 3 0 0 0 1 44 2 0.0 3 0 0 0 2 45 2 0.0 3 0 0 0 2 46 21 0.0 3 0 0 1 20 47 3 0.0 3 0 0 0 3 48 3 0.0 3 0 0 0 3 49 3 0.0 3 0 0 0 3 50 2 0.0 3 0 0 0 2 51 5 0.0 3 0 0 1 4 52 4 0.0 3 0 0 1 3 53 2 0.0 3 0 0 0 2 54 1 0.0 3 0 0 0 1 57 3 0.0 3 0 0 0 3 59 2 0.0 3 0 0 0 2 60 2 0.0 3 0 0 0 2 61 2 0.0 3 0 0 0 2 62 2 0.0 3 0 0 0 2 63 2 0.0 3 0 0 0 2 64 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 0 1 67 4 0.0 3 0 0 0 4 69 1 0.0 3 0 0 0 1 70 4 0.0 3 0 0 0 4 71 13 0.0 3 0 0 0 13 72 12 0.0 3 0 0 0 12 73 1 0.0 3 0 0 0 1 74 4 0.0 3 0 0 0 4 75 1 0.0 3 0 0 0 1 76 4 0.0 3 0 0 0 4 77 6 0.0 3 0 0 0 6 79 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 8 0.0 3 0 0 0 8 82 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 3 0.0 3 0 0 0 3 86 1 0.0 3 0 0 0 1 88 2 0.0 3 0 0 0 2 89 1 0.0 3 0 0 0 1 90 3 0.0 3 0 0 0 3 91 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 0 1 97 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 99 2 0.0 3 0 0 0 2 101 3 0.0 3 0 0 0 3 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 107 2 0.0 3 0 0 1 1 108 1 0.0 3 0 0 0 1 109 2 0.0 3 0 0 0 2 110 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 3 0.0 3 0 0 0 3 114 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 117 2 0.0 3 0 0 0 2 121 1 0.0 3 0 0 0 1 122 9 0.0 3 0 0 0 9 123 2 0.0 3 0 0 1 1 124 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 5 0.0 3 0 0 0 5 129 2 0.0 3 0 0 0 2 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 133 4 0.0 3 0 0 0 4 134 1 0.0 3 0 0 0 1 136 2 0.0 3 0 0 0 2 137 1 0.0 3 0 0 0 1 138 2 0.0 3 0 0 0 2 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 148 3 0.0 3 0 0 0 3 149 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 153 2 0.0 3 0 0 0 2 154 1 0.0 3 0 0 0 1 158 2 0.0 3 0 0 0 2 160 2 0.0 3 0 0 0 2 161 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 3 0.0 3 0 0 0 3 168 2 0.0 3 0 0 0 2 171 1 0.0 3 0 0 0 1 175 2 0.0 3 0 0 0 2 178 2 0.0 3 0 0 0 2 181 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 6 0.0 3 0 0 0 6 187 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 194 8 0.0 3 0 0 0 8 195 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 211 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 222 2 0.0 3 0 0 0 2 229 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 259 1 0.0 3 0 0 0 1 270 5 0.0 3 0 0 0 5 271 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 3 0.0 3 0 0 0 3 283 2 0.0 3 0 0 0 2 284 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 293 1 0.0 3 0 0 0 1 294 1 0.0 3 0 0 0 1 295 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.85 s (58 us/read; 1.04 M reads/minute). === Summary === Total reads processed: 31,925 Reads with adapters: 625 (2.0%) Reads written (passing filters): 31,925 (100.0%) Total basepairs processed: 8,731,623 bp Total written (filtered): 8,729,590 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 625 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 50.9% C: 14.4% G: 31.4% T: 3.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 587 498.8 0 587 4 37 124.7 0 37 124 1 0.0 2 0 0 1