This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCGTCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 179.30 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 311,670 (8.6%) Reads that were too short: 22,537 (0.6%) Reads written (passing filters): 289,133 (8.0%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 77,951,724 bp (7.1%) === Adapter 1 === Sequence: CCGTCC; Type: regular 5'; Length: 6; Trimmed: 311670 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1842 887.3 0 1842 7 11241 887.3 0 11241 8 116853 887.3 0 116853 9 110329 887.3 0 110329 10 184 887.3 0 184 11 51 887.3 0 51 12 51 887.3 0 51 13 38 887.3 0 38 14 68 887.3 0 68 15 35 887.3 0 35 16 42 887.3 0 42 17 49 887.3 0 49 18 54 887.3 0 54 19 42 887.3 0 42 20 64 887.3 0 64 21 60 887.3 0 60 22 42 887.3 0 42 23 48 887.3 0 48 24 58 887.3 0 58 25 47 887.3 0 47 26 37 887.3 0 37 27 53 887.3 0 53 28 58 887.3 0 58 29 81 887.3 0 81 30 40 887.3 0 40 31 30 887.3 0 30 32 37 887.3 0 37 33 37 887.3 0 37 34 80 887.3 0 80 35 57 887.3 0 57 36 66 887.3 0 66 37 75 887.3 0 75 38 57 887.3 0 57 39 67 887.3 0 67 40 52 887.3 0 52 41 61 887.3 0 61 42 63 887.3 0 63 43 39 887.3 0 39 44 40 887.3 0 40 45 40 887.3 0 40 46 70 887.3 0 70 47 38 887.3 0 38 48 44 887.3 0 44 49 60 887.3 0 60 50 51 887.3 0 51 51 48 887.3 0 48 52 53 887.3 0 53 53 87 887.3 0 87 54 115 887.3 0 115 55 108 887.3 0 108 56 65 887.3 0 65 57 53 887.3 0 53 58 98 887.3 0 98 59 79 887.3 0 79 60 68 887.3 0 68 61 56 887.3 0 56 62 64 887.3 0 64 63 128 887.3 0 128 64 159 887.3 0 159 65 126 887.3 0 126 66 63 887.3 0 63 67 99 887.3 0 99 68 126 887.3 0 126 69 116 887.3 0 116 70 72 887.3 0 72 71 117 887.3 0 117 72 587 887.3 0 587 73 1487 887.3 0 1487 74 1624 887.3 0 1624 75 793 887.3 0 793 76 46 887.3 0 46 77 51 887.3 0 51 78 55 887.3 0 55 79 65 887.3 0 65 80 51 887.3 0 51 81 42 887.3 0 42 82 60 887.3 0 60 83 40 887.3 0 40 84 59 887.3 0 59 85 90 887.3 0 90 86 126 887.3 0 126 87 125 887.3 0 125 88 249 887.3 0 249 89 103 887.3 0 103 90 69 887.3 0 69 91 80 887.3 0 80 92 70 887.3 0 70 93 70 887.3 0 70 94 71 887.3 0 71 95 76 887.3 0 76 96 65 887.3 0 65 97 63 887.3 0 63 98 64 887.3 0 64 99 58 887.3 0 58 100 82 887.3 0 82 101 74 887.3 0 74 102 65 887.3 0 65 103 45 887.3 0 45 104 64 887.3 0 64 105 107 887.3 0 107 106 67 887.3 0 67 107 99 887.3 0 99 108 118 887.3 0 118 109 108 887.3 0 108 110 107 887.3 0 107 111 75 887.3 0 75 112 50 887.3 0 50 113 46 887.3 0 46 114 60 887.3 0 60 115 103 887.3 0 103 116 64 887.3 0 64 117 48 887.3 0 48 118 63 887.3 0 63 119 64 887.3 0 64 120 38 887.3 0 38 121 77 887.3 0 77 122 71 887.3 0 71 123 55 887.3 0 55 124 49 887.3 0 49 125 53 887.3 0 53 126 69 887.3 0 69 127 70 887.3 0 70 128 97 887.3 0 97 129 106 887.3 0 106 130 118 887.3 0 118 131 90 887.3 0 90 132 67 887.3 0 67 133 78 887.3 0 78 134 66 887.3 0 66 135 136 887.3 0 136 136 173 887.3 0 173 137 154 887.3 0 154 138 71 887.3 0 71 139 59 887.3 0 59 140 59 887.3 0 59 141 123 887.3 0 123 142 263 887.3 0 263 143 118 887.3 0 118 144 218 887.3 0 218 145 248 887.3 0 248 146 2078 887.3 0 2078 147 1519 887.3 0 1519 148 829 887.3 0 829 149 235 887.3 0 235 150 184 887.3 0 184 151 121 887.3 0 121 152 68 887.3 0 68 153 107 887.3 0 107 154 122 887.3 0 122 155 289 887.3 0 289 156 290 887.3 0 290 157 289 887.3 0 289 158 1275 887.3 0 1275 159 904 887.3 0 904 160 902 887.3 0 902 161 256 887.3 0 256 162 338 887.3 0 338 163 462 887.3 0 462 164 89 887.3 0 89 165 134 887.3 0 134 166 173 887.3 0 173 167 233 887.3 0 233 168 138 887.3 0 138 169 124 887.3 0 124 170 233 887.3 0 233 171 250 887.3 0 250 172 215 887.3 0 215 173 288 887.3 0 288 174 3355 887.3 0 3355 175 4388 887.3 0 4388 176 3020 887.3 0 3020 177 174 887.3 0 174 178 124 887.3 0 124 179 169 887.3 0 169 180 1010 887.3 0 1010 181 2262 887.3 0 2262 182 1339 887.3 0 1339 183 122 887.3 0 122 184 162 887.3 0 162 185 254 887.3 0 254 186 370 887.3 0 370 187 1937 887.3 0 1937 188 1403 887.3 0 1403 189 895 887.3 0 895 190 92 887.3 0 92 191 78 887.3 0 78 192 74 887.3 0 74 193 68 887.3 0 68 194 95 887.3 0 95 195 85 887.3 0 85 196 80 887.3 0 80 197 90 887.3 0 90 198 65 887.3 0 65 199 66 887.3 0 66 200 106 887.3 0 106 201 100 887.3 0 100 202 84 887.3 0 84 203 140 887.3 0 140 204 223 887.3 0 223 205 229 887.3 0 229 206 138 887.3 0 138 207 84 887.3 0 84 208 87 887.3 0 87 209 95 887.3 0 95 210 62 887.3 0 62 211 62 887.3 0 62 212 68 887.3 0 68 213 80 887.3 0 80 214 83 887.3 0 83 215 116 887.3 0 116 216 132 887.3 0 132 217 92 887.3 0 92 218 98 887.3 0 98 219 113 887.3 0 113 220 116 887.3 0 116 221 82 887.3 0 82 222 110 887.3 0 110 223 114 887.3 0 114 224 90 887.3 0 90 225 95 887.3 0 95 226 85 887.3 0 85 227 164 887.3 0 164 228 180 887.3 0 180 229 292 887.3 0 292 230 86 887.3 0 86 231 137 887.3 0 137 232 160 887.3 0 160 233 113 887.3 0 113 234 119 887.3 0 119 235 104 887.3 0 104 236 135 887.3 0 135 237 168 887.3 0 168 238 243 887.3 0 243 239 178 887.3 0 178 240 126 887.3 0 126 241 102 887.3 0 102 242 109 887.3 0 109 243 100 887.3 0 100 244 112 887.3 0 112 245 144 887.3 0 144 246 154 887.3 0 154 247 221 887.3 0 221 248 373 887.3 0 373 249 362 887.3 0 362 250 382 887.3 0 382 251 1645 887.3 0 1645 252 1124 887.3 0 1124 253 2503 887.3 0 2503 254 184 887.3 0 184 255 171 887.3 0 171 256 74 887.3 0 74 257 104 887.3 0 104 258 133 887.3 0 133 259 144 887.3 0 144 260 414 887.3 0 414 261 533 887.3 0 533 262 612 887.3 0 612 263 319 887.3 0 319 264 181 887.3 0 181 265 103 887.3 0 103 266 73 887.3 0 73 267 94 887.3 0 94 268 181 887.3 0 181 269 176 887.3 0 176 270 157 887.3 0 157 271 123 887.3 0 123 272 158 887.3 0 158 273 178 887.3 0 178 274 183 887.3 0 183 275 113 887.3 0 113 276 130 887.3 0 130 277 180 887.3 0 180 278 203 887.3 0 203 279 291 887.3 0 291 280 193 887.3 0 193 281 334 887.3 0 334 282 312 887.3 0 312 283 216 887.3 0 216 284 118 887.3 0 118 285 244 887.3 0 244 286 181 887.3 0 181 287 139 887.3 0 139 288 129 887.3 0 129 289 112 887.3 0 112 290 158 887.3 0 158 291 308 887.3 0 308 292 281 887.3 0 281 293 182 887.3 0 182 294 176 887.3 0 176 295 321 887.3 0 321 296 209 887.3 0 209 297 274 887.3 0 274 298 267 887.3 0 267 299 343 887.3 0 343 300 306 887.3 0 306 301 308 887.3 0 308 Finished in 21.20 s (73 us/read; 0.82 M reads/minute). === Summary === Total reads processed: 289,133 Reads with adapters: 120,848 (41.8%) Reads that were too short: 285 (0.1%) Reads written (passing filters): 120,563 (41.7%) Total basepairs processed: 77,951,724 bp Total written (filtered): 33,265,174 bp (42.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 120848 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 11 0.0 2 0 0 0 11 15 60 0.0 3 0 0 42 18 16 1051 0.0 3 0 923 85 43 17 118397 0.0 3 113335 4326 606 130 18 323 0.0 3 49 227 35 12 19 157 0.0 3 0 0 98 59 20 434 0.0 3 1 0 0 433 21 4 0.0 3 2 1 0 1 23 1 0.0 3 1 24 1 0.0 3 0 0 0 1 25 2 0.0 3 0 0 0 2 27 1 0.0 3 1 28 1 0.0 3 0 0 0 1 29 3 0.0 3 1 0 0 2 30 3 0.0 3 2 0 0 1 31 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 36 7 0.0 3 0 0 0 7 38 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 40 2 0.0 3 0 0 0 2 41 2 0.0 3 2 42 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 46 40 0.0 3 0 0 0 40 48 1 0.0 3 0 0 0 1 49 14 0.0 3 0 0 1 13 52 2 0.0 3 0 0 0 2 54 7 0.0 3 0 0 0 7 55 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 1 58 2 0.0 3 0 0 0 2 59 1 0.0 3 0 0 0 1 62 5 0.0 3 0 0 0 5 66 3 0.0 3 0 0 0 3 67 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 2 0.0 3 0 0 0 2 72 2 0.0 3 0 0 0 2 75 1 0.0 3 0 0 0 1 76 2 0.0 3 0 0 0 2 78 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 0 2 85 5 0.0 3 0 0 0 5 86 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 3 0.0 3 0 0 0 3 99 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 106 105 0.0 3 0 0 1 104 107 1 0.0 3 0 0 0 1 108 2 0.0 3 0 0 0 2 109 2 0.0 3 0 0 0 2 112 1 0.0 3 0 0 0 1 113 2 0.0 3 0 0 0 2 114 1 0.0 3 0 0 0 1 115 2 0.0 3 0 0 0 2 116 1 0.0 3 0 0 0 1 118 2 0.0 3 0 0 0 2 119 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 129 3 0.0 3 0 0 1 2 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 1 137 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 1 156 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 164 3 0.0 3 0 0 1 2 166 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 1 170 2 0.0 3 0 0 0 2 171 4 0.0 3 0 0 0 4 174 2 0.0 3 0 0 0 2 175 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 184 4 0.0 3 0 0 0 4 186 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 191 4 0.0 3 0 1 0 3 192 4 0.0 3 0 0 0 4 194 25 0.0 3 0 1 0 24 195 1 0.0 3 0 0 0 1 204 2 0.0 3 0 0 0 2 208 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 216 2 0.0 3 0 0 0 2 217 2 0.0 3 0 0 0 2 218 2 0.0 3 0 0 0 2 221 2 0.0 3 0 0 1 1 222 2 0.0 3 0 0 0 2 223 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 240 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 270 10 0.0 3 0 0 0 10 271 2 0.0 3 0 0 0 2 273 1 0.0 3 0 0 0 1 278 4 0.0 3 0 0 0 4 279 6 0.0 3 0 0 0 6 280 14 0.0 3 0 0 0 14 281 15 0.0 3 0 0 0 15 282 6 0.0 3 0 0 0 6 283 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 287 2 0.0 3 0 0 0 2 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 7.03 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 120,563 Reads with adapters: 548 (0.5%) Reads written (passing filters): 120,563 (100.0%) Total basepairs processed: 33,265,174 bp Total written (filtered): 33,263,458 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 548 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 56.8% C: 15.3% G: 18.8% T: 9.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 493 1883.8 0 493 4 44 470.9 0 44 5 10 117.7 0 10 11 1 0.0 1 0 1