This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCGTCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 177.32 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 295,612 (8.1%) Reads that were too short: 11,290 (0.3%) Reads written (passing filters): 284,322 (7.8%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 78,880,337 bp (7.2%) === Adapter 1 === Sequence: CCGTCC; Type: regular 5'; Length: 6; Trimmed: 295612 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1826 887.3 0 1826 7 9573 887.3 0 9573 8 115045 887.3 0 115045 9 122688 887.3 0 122688 10 153 887.3 0 153 11 56 887.3 0 56 12 52 887.3 0 52 13 40 887.3 0 40 14 39 887.3 0 39 15 46 887.3 0 46 16 37 887.3 0 37 17 35 887.3 0 35 18 41 887.3 0 41 19 49 887.3 0 49 20 34 887.3 0 34 21 54 887.3 0 54 22 45 887.3 0 45 23 43 887.3 0 43 24 53 887.3 0 53 25 50 887.3 0 50 26 41 887.3 0 41 27 46 887.3 0 46 28 60 887.3 0 60 29 42 887.3 0 42 30 38 887.3 0 38 31 72 887.3 0 72 32 45 887.3 0 45 33 30 887.3 0 30 34 62 887.3 0 62 35 37 887.3 0 37 36 56 887.3 0 56 37 59 887.3 0 59 38 48 887.3 0 48 39 48 887.3 0 48 40 57 887.3 0 57 41 50 887.3 0 50 42 31 887.3 0 31 43 32 887.3 0 32 44 35 887.3 0 35 45 34 887.3 0 34 46 49 887.3 0 49 47 48 887.3 0 48 48 44 887.3 0 44 49 49 887.3 0 49 50 43 887.3 0 43 51 23 887.3 0 23 52 36 887.3 0 36 53 43 887.3 0 43 54 81 887.3 0 81 55 63 887.3 0 63 56 2159 887.3 0 2159 57 70 887.3 0 70 58 56 887.3 0 56 59 39 887.3 0 39 60 55 887.3 0 55 61 44 887.3 0 44 62 41 887.3 0 41 63 42 887.3 0 42 64 62 887.3 0 62 65 66 887.3 0 66 66 36 887.3 0 36 67 92 887.3 0 92 68 80 887.3 0 80 69 73 887.3 0 73 70 70 887.3 0 70 71 100 887.3 0 100 72 708 887.3 0 708 73 1867 887.3 0 1867 74 1800 887.3 0 1800 75 854 887.3 0 854 76 43 887.3 0 43 77 31 887.3 0 31 78 37 887.3 0 37 79 54 887.3 0 54 80 39 887.3 0 39 81 34 887.3 0 34 82 37 887.3 0 37 83 44 887.3 0 44 84 48 887.3 0 48 85 63 887.3 0 63 86 73 887.3 0 73 87 69 887.3 0 69 88 86 887.3 0 86 89 76 887.3 0 76 90 71 887.3 0 71 91 49 887.3 0 49 92 38 887.3 0 38 93 42 887.3 0 42 94 67 887.3 0 67 95 46 887.3 0 46 96 43 887.3 0 43 97 36 887.3 0 36 98 30 887.3 0 30 99 39 887.3 0 39 100 46 887.3 0 46 101 35 887.3 0 35 102 42 887.3 0 42 103 35 887.3 0 35 104 31 887.3 0 31 105 75 887.3 0 75 106 51 887.3 0 51 107 52 887.3 0 52 108 65 887.3 0 65 109 67 887.3 0 67 110 59 887.3 0 59 111 51 887.3 0 51 112 45 887.3 0 45 113 37 887.3 0 37 114 29 887.3 0 29 115 55 887.3 0 55 116 65 887.3 0 65 117 48 887.3 0 48 118 57 887.3 0 57 119 51 887.3 0 51 120 47 887.3 0 47 121 34 887.3 0 34 122 46 887.3 0 46 123 34 887.3 0 34 124 34 887.3 0 34 125 44 887.3 0 44 126 50 887.3 0 50 127 36 887.3 0 36 128 60 887.3 0 60 129 81 887.3 0 81 130 52 887.3 0 52 131 58 887.3 0 58 132 38 887.3 0 38 133 59 887.3 0 59 134 55 887.3 0 55 135 38 887.3 0 38 136 99 887.3 0 99 137 62 887.3 0 62 138 50 887.3 0 50 139 43 887.3 0 43 140 45 887.3 0 45 141 55 887.3 0 55 142 169 887.3 0 169 143 78 887.3 0 78 144 144 887.3 0 144 145 216 887.3 0 216 146 2197 887.3 0 2197 147 1324 887.3 0 1324 148 757 887.3 0 757 149 88 887.3 0 88 150 92 887.3 0 92 151 107 887.3 0 107 152 38 887.3 0 38 153 50 887.3 0 50 154 58 887.3 0 58 155 95 887.3 0 95 156 134 887.3 0 134 157 115 887.3 0 115 158 725 887.3 0 725 159 459 887.3 0 459 160 411 887.3 0 411 161 169 887.3 0 169 162 242 887.3 0 242 163 452 887.3 0 452 164 59 887.3 0 59 165 43 887.3 0 43 166 44 887.3 0 44 167 56 887.3 0 56 168 64 887.3 0 64 169 95 887.3 0 95 170 153 887.3 0 153 171 141 887.3 0 141 172 110 887.3 0 110 173 130 887.3 0 130 174 1585 887.3 0 1585 175 1825 887.3 0 1825 176 1476 887.3 0 1476 177 109 887.3 0 109 178 81 887.3 0 81 179 97 887.3 0 97 180 834 887.3 0 834 181 2196 887.3 0 2196 182 1339 887.3 0 1339 183 44 887.3 0 44 184 76 887.3 0 76 185 75 887.3 0 75 186 129 887.3 0 129 187 923 887.3 0 923 188 572 887.3 0 572 189 389 887.3 0 389 190 62 887.3 0 62 191 53 887.3 0 53 192 45 887.3 0 45 193 60 887.3 0 60 194 47 887.3 0 47 195 35 887.3 0 35 196 39 887.3 0 39 197 36 887.3 0 36 198 44 887.3 0 44 199 40 887.3 0 40 200 57 887.3 0 57 201 53 887.3 0 53 202 31 887.3 0 31 203 40 887.3 0 40 204 132 887.3 0 132 205 135 887.3 0 135 206 86 887.3 0 86 207 50 887.3 0 50 208 41 887.3 0 41 209 36 887.3 0 36 210 34 887.3 0 34 211 52 887.3 0 52 212 41 887.3 0 41 213 33 887.3 0 33 214 50 887.3 0 50 215 84 887.3 0 84 216 43 887.3 0 43 217 45 887.3 0 45 218 68 887.3 0 68 219 59 887.3 0 59 220 56 887.3 0 56 221 59 887.3 0 59 222 38 887.3 0 38 223 59 887.3 0 59 224 59 887.3 0 59 225 61 887.3 0 61 226 35 887.3 0 35 227 82 887.3 0 82 228 116 887.3 0 116 229 154 887.3 0 154 230 75 887.3 0 75 231 84 887.3 0 84 232 63 887.3 0 63 233 70 887.3 0 70 234 62 887.3 0 62 235 57 887.3 0 57 236 76 887.3 0 76 237 88 887.3 0 88 238 104 887.3 0 104 239 103 887.3 0 103 240 54 887.3 0 54 241 33 887.3 0 33 242 53 887.3 0 53 243 47 887.3 0 47 244 61 887.3 0 61 245 61 887.3 0 61 246 55 887.3 0 55 247 82 887.3 0 82 248 119 887.3 0 119 249 140 887.3 0 140 250 166 887.3 0 166 251 1033 887.3 0 1033 252 530 887.3 0 530 253 1378 887.3 0 1378 254 90 887.3 0 90 255 70 887.3 0 70 256 63 887.3 0 63 257 59 887.3 0 59 258 66 887.3 0 66 259 63 887.3 0 63 260 193 887.3 0 193 261 219 887.3 0 219 262 234 887.3 0 234 263 140 887.3 0 140 264 82 887.3 0 82 265 55 887.3 0 55 266 35 887.3 0 35 267 38 887.3 0 38 268 82 887.3 0 82 269 86 887.3 0 86 270 80 887.3 0 80 271 56 887.3 0 56 272 85 887.3 0 85 273 94 887.3 0 94 274 85 887.3 0 85 275 97 887.3 0 97 276 81 887.3 0 81 277 92 887.3 0 92 278 89 887.3 0 89 279 100 887.3 0 100 280 81 887.3 0 81 281 107 887.3 0 107 282 95 887.3 0 95 283 68 887.3 0 68 284 65 887.3 0 65 285 196 887.3 0 196 286 99 887.3 0 99 287 63 887.3 0 63 288 70 887.3 0 70 289 62 887.3 0 62 290 75 887.3 0 75 291 104 887.3 0 104 292 105 887.3 0 105 293 67 887.3 0 67 294 126 887.3 0 126 295 263 887.3 0 263 296 99 887.3 0 99 297 122 887.3 0 122 298 110 887.3 0 110 299 116 887.3 0 116 300 241 887.3 0 241 301 149 887.3 0 149 Finished in 22.37 s (79 us/read; 0.76 M reads/minute). === Summary === Total reads processed: 284,322 Reads with adapters: 130,681 (46.0%) Reads that were too short: 35 (0.0%) Reads written (passing filters): 130,646 (46.0%) Total basepairs processed: 78,880,337 bp Total written (filtered): 35,485,642 bp (45.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 130681 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 4 0.0 3 0 0 0 2 2 18 43 0.0 3 0 0 41 1 1 19 1223 0.0 3 0 1195 25 3 20 128193 0.0 4 126024 2101 55 7 6 21 274 0.0 4 54 209 8 1 2 22 19 0.0 4 1 5 12 1 23 316 0.0 4 234 3 0 72 7 24 5 0.0 4 1 1 1 0 2 25 2 0.0 4 2 29 1 0.0 4 1 30 2 0.0 4 2 36 1 0.0 4 1 51 1 0.0 4 1 57 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 71 1 0.0 4 1 86 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 549 0.0 4 0 0 0 0 549 128 2 0.0 4 0 0 0 0 2 130 2 0.0 4 0 0 0 0 2 135 2 0.0 4 0 0 0 0 2 153 1 0.0 4 0 0 0 0 1 155 7 0.0 4 0 0 0 0 7 156 1 0.0 4 0 0 0 0 1 157 2 0.0 4 0 0 0 0 2 193 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 221 2 0.0 4 0 0 0 0 2 228 3 0.0 4 0 0 0 0 3 229 3 0.0 4 0 0 0 1 2 230 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 235 4 0.0 4 0 0 0 0 4 236 1 0.0 4 0 0 0 0 1 237 3 0.0 4 0 0 0 0 3 244 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 7.77 s (59 us/read; 1.01 M reads/minute). === Summary === Total reads processed: 130,646 Reads with adapters: 13,202 (10.1%) Reads written (passing filters): 130,646 (100.0%) Total basepairs processed: 35,485,642 bp Total written (filtered): 35,354,043 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 13202 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.0% C: 2.7% G: 95.0% T: 1.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 52 2041.3 0 52 4 30 510.3 0 30 5 11 127.6 0 11 6 3 31.9 0 3 8 6 2.0 0 6 9 5830 0.5 0 134 5696 10 4014 0.1 1 146 3868 11 3213 0.0 1 21 3192 12 13 0.0 1 0 13 13 2 0.0 1 0 2 14 2 0.0 1 0 2 18 1 0.0 1 1 19 1 0.0 1 0 1 27 1 0.0 1 1 29 1 0.0 1 1 44 1 0.0 1 0 1 56 1 0.0 1 1 64 1 0.0 1 1 72 1 0.0 1 1 96 1 0.0 1 1 135 1 0.0 1 1 150 13 0.0 1 0 13 152 1 0.0 1 0 1 169 1 0.0 1 1 208 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.