This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAACTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 173.56 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 122,750 (3.4%) Reads that were too short: 22,615 (0.6%) Reads written (passing filters): 100,135 (2.8%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 18,437,473 bp (1.7%) === Adapter 1 === Sequence: GAACTA; Type: regular 5'; Length: 6; Trimmed: 122750 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 652 887.3 0 652 7 16197 887.3 0 16197 8 13904 887.3 0 13904 9 54 887.3 0 54 10 171 887.3 0 171 11 632 887.3 0 632 12 140 887.3 0 140 13 308 887.3 0 308 14 215 887.3 0 215 15 366 887.3 0 366 16 69 887.3 0 69 17 18 887.3 0 18 18 23 887.3 0 23 19 24 887.3 0 24 20 18 887.3 0 18 21 21 887.3 0 21 22 22 887.3 0 22 23 33 887.3 0 33 24 50 887.3 0 50 25 109 887.3 0 109 26 127 887.3 0 127 27 178 887.3 0 178 28 27 887.3 0 27 29 18 887.3 0 18 30 25 887.3 0 25 31 26 887.3 0 26 32 19 887.3 0 19 33 25 887.3 0 25 34 16 887.3 0 16 35 36 887.3 0 36 36 30 887.3 0 30 37 15 887.3 0 15 38 38 887.3 0 38 39 29 887.3 0 29 40 22 887.3 0 22 41 20 887.3 0 20 42 19 887.3 0 19 43 30 887.3 0 30 44 24 887.3 0 24 45 28 887.3 0 28 46 28 887.3 0 28 47 45 887.3 0 45 48 25 887.3 0 25 49 27 887.3 0 27 50 41 887.3 0 41 51 28 887.3 0 28 52 22 887.3 0 22 53 37 887.3 0 37 54 31 887.3 0 31 55 42 887.3 0 42 56 34 887.3 0 34 57 35 887.3 0 35 58 33 887.3 0 33 59 59 887.3 0 59 60 103 887.3 0 103 61 97 887.3 0 97 62 64 887.3 0 64 63 23 887.3 0 23 64 31 887.3 0 31 65 33 887.3 0 33 66 41 887.3 0 41 67 32 887.3 0 32 68 25 887.3 0 25 69 28 887.3 0 28 70 34 887.3 0 34 71 26 887.3 0 26 72 29 887.3 0 29 73 33 887.3 0 33 74 32 887.3 0 32 75 35 887.3 0 35 76 37 887.3 0 37 77 44 887.3 0 44 78 108 887.3 0 108 79 57 887.3 0 57 80 332 887.3 0 332 81 114 887.3 0 114 82 599 887.3 0 599 83 347 887.3 0 347 84 1195 887.3 0 1195 85 28 887.3 0 28 86 47 887.3 0 47 87 49 887.3 0 49 88 30 887.3 0 30 89 67 887.3 0 67 90 34 887.3 0 34 91 25 887.3 0 25 92 28 887.3 0 28 93 68 887.3 0 68 94 26 887.3 0 26 95 63 887.3 0 63 96 98 887.3 0 98 97 54 887.3 0 54 98 40 887.3 0 40 99 86 887.3 0 86 100 60 887.3 0 60 101 44 887.3 0 44 102 91 887.3 0 91 103 113 887.3 0 113 104 144 887.3 0 144 105 79 887.3 0 79 106 87 887.3 0 87 107 374 887.3 0 374 108 41 887.3 0 41 109 34 887.3 0 34 110 121 887.3 0 121 111 94 887.3 0 94 112 133 887.3 0 133 113 105 887.3 0 105 114 98 887.3 0 98 115 60 887.3 0 60 116 45 887.3 0 45 117 45 887.3 0 45 118 79 887.3 0 79 119 91 887.3 0 91 120 110 887.3 0 110 121 76 887.3 0 76 122 173 887.3 0 173 123 233 887.3 0 233 124 143 887.3 0 143 125 72 887.3 0 72 126 83 887.3 0 83 127 70 887.3 0 70 128 80 887.3 0 80 129 60 887.3 0 60 130 71 887.3 0 71 131 54 887.3 0 54 132 80 887.3 0 80 133 50 887.3 0 50 134 118 887.3 0 118 135 99 887.3 0 99 136 92 887.3 0 92 137 146 887.3 0 146 138 186 887.3 0 186 139 141 887.3 0 141 140 162 887.3 0 162 141 167 887.3 0 167 142 134 887.3 0 134 143 65 887.3 0 65 144 71 887.3 0 71 145 91 887.3 0 91 146 122 887.3 0 122 147 146 887.3 0 146 148 100 887.3 0 100 149 92 887.3 0 92 150 77 887.3 0 77 151 85 887.3 0 85 152 98 887.3 0 98 153 98 887.3 0 98 154 93 887.3 0 93 155 157 887.3 0 157 156 123 887.3 0 123 157 166 887.3 0 166 158 180 887.3 0 180 159 359 887.3 0 359 160 1806 887.3 0 1806 161 1661 887.3 0 1661 162 1611 887.3 0 1611 163 211 887.3 0 211 164 109 887.3 0 109 165 94 887.3 0 94 166 84 887.3 0 84 167 83 887.3 0 83 168 103 887.3 0 103 169 99 887.3 0 99 170 186 887.3 0 186 171 64 887.3 0 64 172 88 887.3 0 88 173 105 887.3 0 105 174 121 887.3 0 121 175 227 887.3 0 227 176 137 887.3 0 137 177 143 887.3 0 143 178 105 887.3 0 105 179 121 887.3 0 121 180 426 887.3 0 426 181 758 887.3 0 758 182 1103 887.3 0 1103 183 704 887.3 0 704 184 424 887.3 0 424 185 345 887.3 0 345 186 615 887.3 0 615 187 7548 887.3 0 7548 188 4371 887.3 0 4371 189 30461 887.3 0 30461 190 185 887.3 0 185 191 75 887.3 0 75 192 71 887.3 0 71 193 65 887.3 0 65 194 64 887.3 0 64 195 65 887.3 0 65 196 77 887.3 0 77 197 63 887.3 0 63 198 81 887.3 0 81 199 100 887.3 0 100 200 115 887.3 0 115 201 93 887.3 0 93 202 138 887.3 0 138 203 154 887.3 0 154 204 193 887.3 0 193 205 301 887.3 0 301 206 306 887.3 0 306 207 251 887.3 0 251 208 116 887.3 0 116 209 107 887.3 0 107 210 817 887.3 0 817 211 852 887.3 0 852 212 1087 887.3 0 1087 213 206 887.3 0 206 214 106 887.3 0 106 215 69 887.3 0 69 216 68 887.3 0 68 217 100 887.3 0 100 218 80 887.3 0 80 219 209 887.3 0 209 220 293 887.3 0 293 221 265 887.3 0 265 222 118 887.3 0 118 223 103 887.3 0 103 224 116 887.3 0 116 225 143 887.3 0 143 226 126 887.3 0 126 227 170 887.3 0 170 228 134 887.3 0 134 229 217 887.3 0 217 230 1108 887.3 0 1108 231 1089 887.3 0 1089 232 550 887.3 0 550 233 148 887.3 0 148 234 76 887.3 0 76 235 137 887.3 0 137 236 126 887.3 0 126 237 77 887.3 0 77 238 61 887.3 0 61 239 80 887.3 0 80 240 130 887.3 0 130 241 106 887.3 0 106 242 76 887.3 0 76 243 65 887.3 0 65 244 63 887.3 0 63 245 74 887.3 0 74 246 72 887.3 0 72 247 47 887.3 0 47 248 53 887.3 0 53 249 71 887.3 0 71 250 67 887.3 0 67 251 70 887.3 0 70 252 98 887.3 0 98 253 145 887.3 0 145 254 134 887.3 0 134 255 101 887.3 0 101 256 99 887.3 0 99 257 99 887.3 0 99 258 121 887.3 0 121 259 108 887.3 0 108 260 100 887.3 0 100 261 77 887.3 0 77 262 88 887.3 0 88 263 91 887.3 0 91 264 141 887.3 0 141 265 80 887.3 0 80 266 89 887.3 0 89 267 128 887.3 0 128 268 152 887.3 0 152 269 167 887.3 0 167 270 85 887.3 0 85 271 86 887.3 0 86 272 100 887.3 0 100 273 168 887.3 0 168 274 161 887.3 0 161 275 502 887.3 0 502 276 520 887.3 0 520 277 479 887.3 0 479 278 339 887.3 0 339 279 379 887.3 0 379 280 249 887.3 0 249 281 191 887.3 0 191 282 151 887.3 0 151 283 199 887.3 0 199 284 218 887.3 0 218 285 223 887.3 0 223 286 113 887.3 0 113 287 217 887.3 0 217 288 319 887.3 0 319 289 749 887.3 0 749 290 605 887.3 0 605 291 705 887.3 0 705 292 86 887.3 0 86 293 125 887.3 0 125 294 144 887.3 0 144 295 145 887.3 0 145 296 98 887.3 0 98 297 121 887.3 0 121 298 188 887.3 0 188 299 241 887.3 0 241 300 770 887.3 0 770 301 730 887.3 0 730 Finished in 6.06 s (61 us/read; 0.99 M reads/minute). === Summary === Total reads processed: 100,135 Reads with adapters: 16,295 (16.3%) Reads that were too short: 141 (0.1%) Reads written (passing filters): 16,154 (16.1%) Total basepairs processed: 18,437,473 bp Total written (filtered): 4,449,953 bp (24.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 16295 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 17 0.0 3 0 0 10 7 16 200 0.0 3 0 168 27 5 17 15627 0.0 3 14794 685 120 28 18 37 0.0 3 2 33 2 19 77 0.0 3 21 15 23 18 20 38 0.0 3 2 0 0 36 22 4 0.0 3 0 1 0 3 25 4 0.0 3 0 0 0 4 27 1 0.0 3 0 0 0 1 29 1 0.0 3 1 30 1 0.0 3 0 0 0 1 33 8 0.0 3 0 0 0 8 34 2 0.0 3 0 0 0 2 35 2 0.0 3 0 0 0 2 37 6 0.0 3 0 0 0 6 39 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 44 3 0.0 3 0 0 0 3 49 1 0.0 3 0 0 0 1 54 12 0.0 3 0 0 0 12 57 3 0.0 3 0 0 0 3 58 2 0.0 3 0 0 0 2 59 2 0.0 3 0 0 0 2 61 2 0.0 3 0 0 1 1 62 6 0.0 3 0 0 0 6 66 1 0.0 3 0 0 0 1 69 7 0.0 3 0 0 0 7 70 4 0.0 3 0 0 0 4 71 4 0.0 3 0 0 0 4 74 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 90 8 0.0 3 0 0 0 8 91 3 0.0 3 0 0 0 3 93 2 0.0 3 0 0 0 2 96 1 0.0 3 0 0 0 1 98 3 0.0 3 0 0 0 3 100 5 0.0 3 0 0 0 5 101 3 0.0 3 0 0 0 3 104 1 0.0 3 0 0 0 1 105 2 0.0 3 0 0 1 1 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 109 3 0.0 3 0 0 0 3 110 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 4 0.0 3 0 0 0 4 114 4 0.0 3 0 0 0 4 116 2 0.0 3 0 0 0 2 120 2 0.0 3 0 0 0 2 121 1 0.0 3 0 0 0 1 123 9 0.0 3 0 0 0 9 124 4 0.0 3 0 0 0 4 125 2 0.0 3 1 0 0 1 126 5 0.0 3 0 0 0 5 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 131 5 0.0 3 0 0 0 5 133 3 0.0 3 0 0 0 3 135 3 0.0 3 0 0 0 3 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 140 2 0.0 3 0 0 0 2 141 53 0.0 3 0 0 0 53 142 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 151 3 0.0 3 0 0 0 3 154 2 0.0 3 0 0 0 2 155 3 0.0 3 0 0 1 2 156 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 7 0.0 3 0 0 0 7 161 1 0.0 3 0 0 0 1 162 2 0.0 3 0 0 0 2 163 3 0.0 3 0 0 0 3 165 3 0.0 3 0 0 0 3 171 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 181 3 0.0 3 0 0 0 3 182 1 0.0 3 0 0 0 1 183 3 0.0 3 0 0 0 3 184 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 216 2 0.0 3 0 0 0 2 217 1 0.0 3 0 0 0 1 218 2 0.0 3 0 0 0 2 231 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 0 1 264 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 274 1 0.0 3 0 0 0 1 275 2 0.0 3 0 0 0 2 278 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.97 s (60 us/read; 1.00 M reads/minute). === Summary === Total reads processed: 16,154 Reads with adapters: 78 (0.5%) Reads written (passing filters): 16,154 (100.0%) Total basepairs processed: 4,449,953 bp Total written (filtered): 4,449,713 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 78 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 29.5% C: 28.2% G: 34.6% T: 7.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 75 252.4 0 75 5 3 15.8 0 3