This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAACTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 174.13 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 113,062 (3.1%) Reads that were too short: 15,175 (0.4%) Reads written (passing filters): 97,887 (2.7%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 18,009,536 bp (1.6%) === Adapter 1 === Sequence: GAACTA; Type: regular 5'; Length: 6; Trimmed: 113062 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 439 887.3 0 439 7 14835 887.3 0 14835 8 16142 887.3 0 16142 9 28 887.3 0 28 10 30 887.3 0 30 11 167 887.3 0 167 12 39 887.3 0 39 13 87 887.3 0 87 14 138 887.3 0 138 15 158 887.3 0 158 16 55 887.3 0 55 17 11 887.3 0 11 18 15 887.3 0 15 19 12 887.3 0 12 20 14 887.3 0 14 21 15 887.3 0 15 22 7 887.3 0 7 23 17 887.3 0 17 24 19 887.3 0 19 25 980 887.3 0 980 26 59 887.3 0 59 27 20 887.3 0 20 28 13 887.3 0 13 29 14 887.3 0 14 30 13 887.3 0 13 31 15 887.3 0 15 32 20 887.3 0 20 33 19 887.3 0 19 34 18 887.3 0 18 35 15 887.3 0 15 36 23 887.3 0 23 37 9 887.3 0 9 38 10 887.3 0 10 39 14 887.3 0 14 40 13 887.3 0 13 41 44 887.3 0 44 42 19 887.3 0 19 43 17 887.3 0 17 44 25 887.3 0 25 45 19 887.3 0 19 46 20 887.3 0 20 47 19 887.3 0 19 48 15 887.3 0 15 49 19 887.3 0 19 50 19 887.3 0 19 51 24 887.3 0 24 52 21 887.3 0 21 53 24 887.3 0 24 54 15 887.3 0 15 55 28 887.3 0 28 56 34 887.3 0 34 57 45 887.3 0 45 58 24 887.3 0 24 59 45 887.3 0 45 60 78 887.3 0 78 61 55 887.3 0 55 62 46 887.3 0 46 63 17 887.3 0 17 64 20 887.3 0 20 65 27 887.3 0 27 66 25 887.3 0 25 67 18 887.3 0 18 68 13 887.3 0 13 69 18 887.3 0 18 70 24 887.3 0 24 71 26 887.3 0 26 72 14 887.3 0 14 73 16 887.3 0 16 74 28 887.3 0 28 75 27 887.3 0 27 76 28 887.3 0 28 77 24 887.3 0 24 78 29 887.3 0 29 79 50 887.3 0 50 80 67 887.3 0 67 81 129 887.3 0 129 82 940 887.3 0 940 83 508 887.3 0 508 84 1910 887.3 0 1910 85 42 887.3 0 42 86 67 887.3 0 67 87 70 887.3 0 70 88 46 887.3 0 46 89 87 887.3 0 87 90 61 887.3 0 61 91 46 887.3 0 46 92 35 887.3 0 35 93 55 887.3 0 55 94 54 887.3 0 54 95 71 887.3 0 71 96 66 887.3 0 66 97 47 887.3 0 47 98 60 887.3 0 60 99 40 887.3 0 40 100 47 887.3 0 47 101 69 887.3 0 69 102 60 887.3 0 60 103 58 887.3 0 58 104 95 887.3 0 95 105 81 887.3 0 81 106 85 887.3 0 85 107 395 887.3 0 395 108 31 887.3 0 31 109 52 887.3 0 52 110 116 887.3 0 116 111 76 887.3 0 76 112 86 887.3 0 86 113 73 887.3 0 73 114 69 887.3 0 69 115 53 887.3 0 53 116 45 887.3 0 45 117 45 887.3 0 45 118 79 887.3 0 79 119 71 887.3 0 71 120 97 887.3 0 97 121 67 887.3 0 67 122 123 887.3 0 123 123 152 887.3 0 152 124 99 887.3 0 99 125 74 887.3 0 74 126 86 887.3 0 86 127 77 887.3 0 77 128 59 887.3 0 59 129 61 887.3 0 61 130 57 887.3 0 57 131 86 887.3 0 86 132 76 887.3 0 76 133 49 887.3 0 49 134 91 887.3 0 91 135 55 887.3 0 55 136 64 887.3 0 64 137 86 887.3 0 86 138 87 887.3 0 87 139 95 887.3 0 95 140 124 887.3 0 124 141 112 887.3 0 112 142 102 887.3 0 102 143 69 887.3 0 69 144 81 887.3 0 81 145 86 887.3 0 86 146 88 887.3 0 88 147 111 887.3 0 111 148 59 887.3 0 59 149 68 887.3 0 68 150 78 887.3 0 78 151 51 887.3 0 51 152 60 887.3 0 60 153 83 887.3 0 83 154 82 887.3 0 82 155 71 887.3 0 71 156 78 887.3 0 78 157 80 887.3 0 80 158 112 887.3 0 112 159 134 887.3 0 134 160 507 887.3 0 507 161 545 887.3 0 545 162 569 887.3 0 569 163 108 887.3 0 108 164 60 887.3 0 60 165 60 887.3 0 60 166 71 887.3 0 71 167 67 887.3 0 67 168 66 887.3 0 66 169 103 887.3 0 103 170 173 887.3 0 173 171 75 887.3 0 75 172 73 887.3 0 73 173 100 887.3 0 100 174 103 887.3 0 103 175 163 887.3 0 163 176 102 887.3 0 102 177 83 887.3 0 83 178 74 887.3 0 74 179 82 887.3 0 82 180 207 887.3 0 207 181 278 887.3 0 278 182 416 887.3 0 416 183 259 887.3 0 259 184 366 887.3 0 366 185 284 887.3 0 284 186 538 887.3 0 538 187 8251 887.3 0 8251 188 4396 887.3 0 4396 189 34835 887.3 0 34835 190 165 887.3 0 165 191 70 887.3 0 70 192 51 887.3 0 51 193 52 887.3 0 52 194 60 887.3 0 60 195 54 887.3 0 54 196 55 887.3 0 55 197 72 887.3 0 72 198 80 887.3 0 80 199 70 887.3 0 70 200 74 887.3 0 74 201 71 887.3 0 71 202 100 887.3 0 100 203 104 887.3 0 104 204 93 887.3 0 93 205 145 887.3 0 145 206 132 887.3 0 132 207 148 887.3 0 148 208 83 887.3 0 83 209 57 887.3 0 57 210 307 887.3 0 307 211 321 887.3 0 321 212 468 887.3 0 468 213 120 887.3 0 120 214 87 887.3 0 87 215 81 887.3 0 81 216 70 887.3 0 70 217 59 887.3 0 59 218 71 887.3 0 71 219 121 887.3 0 121 220 221 887.3 0 221 221 176 887.3 0 176 222 79 887.3 0 79 223 74 887.3 0 74 224 86 887.3 0 86 225 98 887.3 0 98 226 77 887.3 0 77 227 124 887.3 0 124 228 83 887.3 0 83 229 125 887.3 0 125 230 587 887.3 0 587 231 558 887.3 0 558 232 277 887.3 0 277 233 119 887.3 0 119 234 63 887.3 0 63 235 110 887.3 0 110 236 105 887.3 0 105 237 63 887.3 0 63 238 56 887.3 0 56 239 98 887.3 0 98 240 94 887.3 0 94 241 97 887.3 0 97 242 75 887.3 0 75 243 64 887.3 0 64 244 84 887.3 0 84 245 89 887.3 0 89 246 66 887.3 0 66 247 58 887.3 0 58 248 55 887.3 0 55 249 64 887.3 0 64 250 56 887.3 0 56 251 73 887.3 0 73 252 89 887.3 0 89 253 105 887.3 0 105 254 97 887.3 0 97 255 83 887.3 0 83 256 81 887.3 0 81 257 73 887.3 0 73 258 96 887.3 0 96 259 67 887.3 0 67 260 92 887.3 0 92 261 71 887.3 0 71 262 68 887.3 0 68 263 80 887.3 0 80 264 117 887.3 0 117 265 68 887.3 0 68 266 78 887.3 0 78 267 66 887.3 0 66 268 79 887.3 0 79 269 107 887.3 0 107 270 56 887.3 0 56 271 78 887.3 0 78 272 70 887.3 0 70 273 123 887.3 0 123 274 146 887.3 0 146 275 460 887.3 0 460 276 505 887.3 0 505 277 463 887.3 0 463 278 259 887.3 0 259 279 224 887.3 0 224 280 160 887.3 0 160 281 125 887.3 0 125 282 83 887.3 0 83 283 112 887.3 0 112 284 145 887.3 0 145 285 134 887.3 0 134 286 86 887.3 0 86 287 122 887.3 0 122 288 259 887.3 0 259 289 484 887.3 0 484 290 402 887.3 0 402 291 506 887.3 0 506 292 74 887.3 0 74 293 103 887.3 0 103 294 101 887.3 0 101 295 103 887.3 0 103 296 104 887.3 0 104 297 148 887.3 0 148 298 178 887.3 0 178 299 234 887.3 0 234 300 702 887.3 0 702 301 288 887.3 0 288 Finished in 6.36 s (65 us/read; 0.92 M reads/minute). === Summary === Total reads processed: 97,887 Reads with adapters: 17,711 (18.1%) Reads that were too short: 20 (0.0%) Reads written (passing filters): 17,691 (18.1%) Total basepairs processed: 18,009,536 bp Total written (filtered): 4,731,823 bp (26.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 17711 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 5 0.0 3 0 0 5 19 172 0.0 3 0 166 5 0 1 20 16515 0.0 4 16228 279 8 21 52 0.0 4 15 37 22 9 0.0 4 3 0 6 23 9 0.0 4 0 0 0 8 1 24 1 0.0 4 0 0 0 0 1 28 1 0.0 4 1 40 1 0.0 4 0 0 0 0 1 51 1 0.0 4 0 0 0 0 1 53 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 5 0.0 4 0 0 0 0 5 127 915 0.0 4 2 0 0 0 913 128 4 0.0 4 1 0 0 0 3 136 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 204 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 230 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.03 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 17,691 Reads with adapters: 1,438 (8.1%) Reads written (passing filters): 17,691 (100.0%) Total basepairs processed: 4,731,823 bp Total written (filtered): 4,715,321 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1438 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.5% C: 3.4% G: 93.9% T: 1.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 9 276.4 0 9 4 29 69.1 0 29 7 1 1.1 0 1 9 36 0.1 0 1 35 10 121 0.0 1 3 118 11 1234 0.0 1 9 1225 12 3 0.0 1 0 3 224 3 0.0 1 3 268 2 0.0 1 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.