This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCACTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 172.91 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 133,950 (3.7%) Reads that were too short: 33,646 (0.9%) Reads written (passing filters): 100,304 (2.8%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 20,236,364 bp (1.8%) === Adapter 1 === Sequence: TCACTC; Type: regular 5'; Length: 6; Trimmed: 133950 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1395 887.3 0 1395 7 15249 887.3 0 15249 8 940 887.3 0 940 9 18181 887.3 0 18181 10 43 887.3 0 43 11 29 887.3 0 29 12 68 887.3 0 68 13 47 887.3 0 47 14 30 887.3 0 30 15 24 887.3 0 24 16 36 887.3 0 36 17 41 887.3 0 41 18 24 887.3 0 24 19 35 887.3 0 35 20 31 887.3 0 31 21 23 887.3 0 23 22 23 887.3 0 23 23 30 887.3 0 30 24 25 887.3 0 25 25 27 887.3 0 27 26 24 887.3 0 24 27 37 887.3 0 37 28 37 887.3 0 37 29 36 887.3 0 36 30 38 887.3 0 38 31 36 887.3 0 36 32 42 887.3 0 42 33 21 887.3 0 21 34 32 887.3 0 32 35 51 887.3 0 51 36 44 887.3 0 44 37 52 887.3 0 52 38 311 887.3 0 311 39 172 887.3 0 172 40 181 887.3 0 181 41 65 887.3 0 65 42 37 887.3 0 37 43 60 887.3 0 60 44 27 887.3 0 27 45 43 887.3 0 43 46 27 887.3 0 27 47 50 887.3 0 50 48 25 887.3 0 25 49 30 887.3 0 30 50 52 887.3 0 52 51 39 887.3 0 39 52 34 887.3 0 34 53 171 887.3 0 171 54 305 887.3 0 305 55 134 887.3 0 134 56 76 887.3 0 76 57 222 887.3 0 222 58 73 887.3 0 73 59 23 887.3 0 23 60 65 887.3 0 65 61 40 887.3 0 40 62 28 887.3 0 28 63 34 887.3 0 34 64 35 887.3 0 35 65 46 887.3 0 46 66 107 887.3 0 107 67 173 887.3 0 173 68 117 887.3 0 117 69 82 887.3 0 82 70 110 887.3 0 110 71 47 887.3 0 47 72 62 887.3 0 62 73 46 887.3 0 46 74 72 887.3 0 72 75 22 887.3 0 22 76 18 887.3 0 18 77 28 887.3 0 28 78 35 887.3 0 35 79 29 887.3 0 29 80 30 887.3 0 30 81 35 887.3 0 35 82 45 887.3 0 45 83 32 887.3 0 32 84 46 887.3 0 46 85 32 887.3 0 32 86 40 887.3 0 40 87 48 887.3 0 48 88 35 887.3 0 35 89 59 887.3 0 59 90 29 887.3 0 29 91 89 887.3 0 89 92 42 887.3 0 42 93 78 887.3 0 78 94 66 887.3 0 66 95 84 887.3 0 84 96 47 887.3 0 47 97 39 887.3 0 39 98 31 887.3 0 31 99 42 887.3 0 42 100 19 887.3 0 19 101 44 887.3 0 44 102 71 887.3 0 71 103 114 887.3 0 114 104 145 887.3 0 145 105 241 887.3 0 241 106 94 887.3 0 94 107 116 887.3 0 116 108 72 887.3 0 72 109 71 887.3 0 71 110 56 887.3 0 56 111 116 887.3 0 116 112 123 887.3 0 123 113 195 887.3 0 195 114 90 887.3 0 90 115 95 887.3 0 95 116 75 887.3 0 75 117 64 887.3 0 64 118 52 887.3 0 52 119 68 887.3 0 68 120 84 887.3 0 84 121 71 887.3 0 71 122 79 887.3 0 79 123 115 887.3 0 115 124 137 887.3 0 137 125 1094 887.3 0 1094 126 1465 887.3 0 1465 127 1747 887.3 0 1747 128 87 887.3 0 87 129 74 887.3 0 74 130 126 887.3 0 126 131 80 887.3 0 80 132 96 887.3 0 96 133 177 887.3 0 177 134 134 887.3 0 134 135 128 887.3 0 128 136 142 887.3 0 142 137 124 887.3 0 124 138 114 887.3 0 114 139 108 887.3 0 108 140 142 887.3 0 142 141 102 887.3 0 102 142 111 887.3 0 111 143 150 887.3 0 150 144 620 887.3 0 620 145 801 887.3 0 801 146 818 887.3 0 818 147 356 887.3 0 356 148 141 887.3 0 141 149 158 887.3 0 158 150 502 887.3 0 502 151 606 887.3 0 606 152 657 887.3 0 657 153 225 887.3 0 225 154 319 887.3 0 319 155 956 887.3 0 956 156 1069 887.3 0 1069 157 1320 887.3 0 1320 158 1383 887.3 0 1383 159 2288 887.3 0 2288 160 9984 887.3 0 9984 161 8177 887.3 0 8177 162 7642 887.3 0 7642 163 185 887.3 0 185 164 191 887.3 0 191 165 157 887.3 0 157 166 174 887.3 0 174 167 115 887.3 0 115 168 102 887.3 0 102 169 149 887.3 0 149 170 145 887.3 0 145 171 181 887.3 0 181 172 123 887.3 0 123 173 153 887.3 0 153 174 181 887.3 0 181 175 658 887.3 0 658 176 418 887.3 0 418 177 426 887.3 0 426 178 222 887.3 0 222 179 323 887.3 0 323 180 2049 887.3 0 2049 181 2335 887.3 0 2335 182 1627 887.3 0 1627 183 687 887.3 0 687 184 111 887.3 0 111 185 144 887.3 0 144 186 129 887.3 0 129 187 132 887.3 0 132 188 121 887.3 0 121 189 111 887.3 0 111 190 132 887.3 0 132 191 127 887.3 0 127 192 80 887.3 0 80 193 58 887.3 0 58 194 71 887.3 0 71 195 108 887.3 0 108 196 83 887.3 0 83 197 107 887.3 0 107 198 109 887.3 0 109 199 169 887.3 0 169 200 115 887.3 0 115 201 113 887.3 0 113 202 89 887.3 0 89 203 112 887.3 0 112 204 189 887.3 0 189 205 98 887.3 0 98 206 102 887.3 0 102 207 103 887.3 0 103 208 158 887.3 0 158 209 108 887.3 0 108 210 153 887.3 0 153 211 318 887.3 0 318 212 1701 887.3 0 1701 213 1830 887.3 0 1830 214 1403 887.3 0 1403 215 132 887.3 0 132 216 126 887.3 0 126 217 150 887.3 0 150 218 132 887.3 0 132 219 173 887.3 0 173 220 220 887.3 0 220 221 208 887.3 0 208 222 134 887.3 0 134 223 172 887.3 0 172 224 259 887.3 0 259 225 220 887.3 0 220 226 193 887.3 0 193 227 148 887.3 0 148 228 127 887.3 0 127 229 142 887.3 0 142 230 111 887.3 0 111 231 118 887.3 0 118 232 89 887.3 0 89 233 109 887.3 0 109 234 169 887.3 0 169 235 142 887.3 0 142 236 130 887.3 0 130 237 140 887.3 0 140 238 231 887.3 0 231 239 316 887.3 0 316 240 211 887.3 0 211 241 224 887.3 0 224 242 176 887.3 0 176 243 287 887.3 0 287 244 222 887.3 0 222 245 262 887.3 0 262 246 251 887.3 0 251 247 306 887.3 0 306 248 296 887.3 0 296 249 168 887.3 0 168 250 181 887.3 0 181 251 166 887.3 0 166 252 199 887.3 0 199 253 178 887.3 0 178 254 195 887.3 0 195 255 364 887.3 0 364 256 954 887.3 0 954 257 1275 887.3 0 1275 258 1070 887.3 0 1070 259 296 887.3 0 296 260 207 887.3 0 207 261 205 887.3 0 205 262 167 887.3 0 167 263 160 887.3 0 160 264 163 887.3 0 163 265 161 887.3 0 161 266 478 887.3 0 478 267 854 887.3 0 854 268 1019 887.3 0 1019 269 384 887.3 0 384 270 256 887.3 0 256 271 327 887.3 0 327 272 1097 887.3 0 1097 273 1398 887.3 0 1398 274 980 887.3 0 980 275 596 887.3 0 596 276 240 887.3 0 240 277 240 887.3 0 240 278 299 887.3 0 299 279 289 887.3 0 289 280 463 887.3 0 463 281 431 887.3 0 431 282 413 887.3 0 413 283 285 887.3 0 285 284 237 887.3 0 237 285 253 887.3 0 253 286 253 887.3 0 253 287 573 887.3 0 573 288 499 887.3 0 499 289 498 887.3 0 498 290 167 887.3 0 167 291 169 887.3 0 169 292 213 887.3 0 213 293 306 887.3 0 306 294 271 887.3 0 271 295 176 887.3 0 176 296 120 887.3 0 120 297 158 887.3 0 158 298 177 887.3 0 177 299 191 887.3 0 191 300 220 887.3 0 220 301 317 887.3 0 317 Finished in 6.41 s (64 us/read; 0.94 M reads/minute). === Summary === Total reads processed: 100,304 Reads with adapters: 19,269 (19.2%) Reads that were too short: 125 (0.1%) Reads written (passing filters): 19,144 (19.1%) Total basepairs processed: 20,236,364 bp Total written (filtered): 5,256,921 bp (26.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 19269 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 7 0.0 3 0 0 6 1 16 158 0.0 3 0 136 13 9 17 18219 0.0 3 17207 824 157 31 18 621 0.0 3 540 71 7 3 19 34 0.0 3 10 3 11 10 20 46 0.0 3 0 0 1 45 21 2 0.0 3 0 0 0 2 22 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 33 4 0.0 3 0 0 0 4 34 5 0.0 3 0 0 0 5 35 8 0.0 3 0 0 0 8 37 8 0.0 3 0 0 1 7 40 1 0.0 3 0 0 0 1 41 3 0.0 3 0 0 0 3 47 4 0.0 3 0 0 0 4 49 1 0.0 3 0 0 0 1 50 4 0.0 3 0 0 0 4 51 1 0.0 3 0 0 1 52 1 0.0 3 0 0 0 1 55 2 0.0 3 0 0 0 2 57 1 0.0 3 0 0 0 1 58 2 0.0 3 0 0 0 2 60 6 0.0 3 0 0 0 6 62 8 0.0 3 0 0 0 8 63 3 0.0 3 0 0 0 3 64 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 0 1 68 2 0.0 3 0 0 0 2 69 1 0.0 3 0 0 0 1 71 9 0.0 3 0 0 0 9 73 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 75 6 0.0 3 0 1 1 4 80 2 0.0 3 0 0 0 2 81 1 0.0 3 0 0 0 1 85 2 0.0 3 0 0 0 2 88 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 0 2 97 1 0.0 3 0 0 0 1 98 2 0.0 3 0 2 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 111 2 0.0 3 0 0 0 2 112 4 0.0 3 0 0 0 4 113 10 0.0 3 0 0 2 8 119 2 0.0 3 0 0 0 2 121 1 0.0 3 0 0 0 1 123 3 0.0 3 0 0 0 3 124 1 0.0 3 0 0 0 1 126 3 0.0 3 0 0 0 3 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 2 0.0 3 0 0 0 2 131 4 0.0 3 0 0 0 4 133 1 0.0 3 0 0 0 1 136 3 0.0 3 0 0 0 3 137 2 0.0 3 0 0 0 2 139 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 163 5 0.0 3 0 0 0 5 164 1 0.0 3 0 0 0 1 165 2 0.0 3 0 0 0 2 166 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 1 177 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 264 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 5 0.0 3 0 0 0 5 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.16 s (61 us/read; 0.99 M reads/minute). === Summary === Total reads processed: 19,144 Reads with adapters: 769 (4.0%) Reads written (passing filters): 19,144 (100.0%) Total basepairs processed: 5,256,921 bp Total written (filtered): 5,254,593 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 769 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 60.1% C: 14.6% G: 22.9% T: 2.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 750 299.1 0 750 4 18 74.8 0 18 6 1 4.7 0 1