This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCACTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 173.48 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 109,344 (3.0%) Reads that were too short: 21,144 (0.6%) Reads written (passing filters): 88,200 (2.4%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 18,644,807 bp (1.7%) === Adapter 1 === Sequence: TCACTC; Type: regular 5'; Length: 6; Trimmed: 109344 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1309 887.3 0 1309 7 18435 887.3 0 18435 8 774 887.3 0 774 9 16118 887.3 0 16118 10 26 887.3 0 26 11 26 887.3 0 26 12 32 887.3 0 32 13 27 887.3 0 27 14 35 887.3 0 35 15 22 887.3 0 22 16 19 887.3 0 19 17 26 887.3 0 26 18 25 887.3 0 25 19 21 887.3 0 21 20 24 887.3 0 24 21 33 887.3 0 33 22 26 887.3 0 26 23 20 887.3 0 20 24 25 887.3 0 25 25 23 887.3 0 23 26 21 887.3 0 21 27 23 887.3 0 23 28 31 887.3 0 31 29 35 887.3 0 35 30 24 887.3 0 24 31 24 887.3 0 24 32 23 887.3 0 23 33 23 887.3 0 23 34 102 887.3 0 102 35 55 887.3 0 55 36 34 887.3 0 34 37 45 887.3 0 45 38 268 887.3 0 268 39 213 887.3 0 213 40 196 887.3 0 196 41 40 887.3 0 40 42 15 887.3 0 15 43 25 887.3 0 25 44 22 887.3 0 22 45 284 887.3 0 284 46 34 887.3 0 34 47 25 887.3 0 25 48 21 887.3 0 21 49 15 887.3 0 15 50 39 887.3 0 39 51 23 887.3 0 23 52 19 887.3 0 19 53 173 887.3 0 173 54 303 887.3 0 303 55 121 887.3 0 121 56 32 887.3 0 32 57 211 887.3 0 211 58 29 887.3 0 29 59 22 887.3 0 22 60 44 887.3 0 44 61 31 887.3 0 31 62 25 887.3 0 25 63 43 887.3 0 43 64 36 887.3 0 36 65 28 887.3 0 28 66 39 887.3 0 39 67 57 887.3 0 57 68 34 887.3 0 34 69 93 887.3 0 93 70 113 887.3 0 113 71 35 887.3 0 35 72 29 887.3 0 29 73 29 887.3 0 29 74 24 887.3 0 24 75 14 887.3 0 14 76 27 887.3 0 27 77 23 887.3 0 23 78 21 887.3 0 21 79 24 887.3 0 24 80 18 887.3 0 18 81 27 887.3 0 27 82 22 887.3 0 22 83 38 887.3 0 38 84 29 887.3 0 29 85 30 887.3 0 30 86 40 887.3 0 40 87 39 887.3 0 39 88 65 887.3 0 65 89 63 887.3 0 63 90 46 887.3 0 46 91 83 887.3 0 83 92 56 887.3 0 56 93 68 887.3 0 68 94 62 887.3 0 62 95 71 887.3 0 71 96 43 887.3 0 43 97 34 887.3 0 34 98 39 887.3 0 39 99 30 887.3 0 30 100 35 887.3 0 35 101 51 887.3 0 51 102 48 887.3 0 48 103 51 887.3 0 51 104 109 887.3 0 109 105 104 887.3 0 104 106 75 887.3 0 75 107 62 887.3 0 62 108 54 887.3 0 54 109 58 887.3 0 58 110 41 887.3 0 41 111 72 887.3 0 72 112 73 887.3 0 73 113 129 887.3 0 129 114 51 887.3 0 51 115 69 887.3 0 69 116 60 887.3 0 60 117 45 887.3 0 45 118 56 887.3 0 56 119 59 887.3 0 59 120 84 887.3 0 84 121 68 887.3 0 68 122 77 887.3 0 77 123 114 887.3 0 114 124 104 887.3 0 104 125 1083 887.3 0 1083 126 1469 887.3 0 1469 127 1994 887.3 0 1994 128 61 887.3 0 61 129 61 887.3 0 61 130 80 887.3 0 80 131 67 887.3 0 67 132 49 887.3 0 49 133 127 887.3 0 127 134 91 887.3 0 91 135 72 887.3 0 72 136 113 887.3 0 113 137 61 887.3 0 61 138 51 887.3 0 51 139 45 887.3 0 45 140 61 887.3 0 61 141 73 887.3 0 73 142 82 887.3 0 82 143 108 887.3 0 108 144 369 887.3 0 369 145 489 887.3 0 489 146 468 887.3 0 468 147 206 887.3 0 206 148 101 887.3 0 101 149 100 887.3 0 100 150 361 887.3 0 361 151 430 887.3 0 430 152 414 887.3 0 414 153 137 887.3 0 137 154 187 887.3 0 187 155 462 887.3 0 462 156 537 887.3 0 537 157 631 887.3 0 631 158 698 887.3 0 698 159 1180 887.3 0 1180 160 10223 887.3 0 10223 161 8066 887.3 0 8066 162 7455 887.3 0 7455 163 127 887.3 0 127 164 133 887.3 0 133 165 88 887.3 0 88 166 104 887.3 0 104 167 47 887.3 0 47 168 61 887.3 0 61 169 104 887.3 0 104 170 71 887.3 0 71 171 147 887.3 0 147 172 65 887.3 0 65 173 63 887.3 0 63 174 92 887.3 0 92 175 290 887.3 0 290 176 182 887.3 0 182 177 197 887.3 0 197 178 91 887.3 0 91 179 125 887.3 0 125 180 1023 887.3 0 1023 181 1450 887.3 0 1450 182 806 887.3 0 806 183 372 887.3 0 372 184 66 887.3 0 66 185 109 887.3 0 109 186 80 887.3 0 80 187 84 887.3 0 84 188 77 887.3 0 77 189 62 887.3 0 62 190 63 887.3 0 63 191 57 887.3 0 57 192 62 887.3 0 62 193 51 887.3 0 51 194 52 887.3 0 52 195 64 887.3 0 64 196 48 887.3 0 48 197 88 887.3 0 88 198 74 887.3 0 74 199 88 887.3 0 88 200 74 887.3 0 74 201 64 887.3 0 64 202 65 887.3 0 65 203 89 887.3 0 89 204 180 887.3 0 180 205 58 887.3 0 58 206 54 887.3 0 54 207 83 887.3 0 83 208 86 887.3 0 86 209 84 887.3 0 84 210 119 887.3 0 119 211 244 887.3 0 244 212 1821 887.3 0 1821 213 1762 887.3 0 1762 214 1445 887.3 0 1445 215 72 887.3 0 72 216 63 887.3 0 63 217 89 887.3 0 89 218 90 887.3 0 90 219 93 887.3 0 93 220 156 887.3 0 156 221 144 887.3 0 144 222 73 887.3 0 73 223 71 887.3 0 71 224 98 887.3 0 98 225 109 887.3 0 109 226 114 887.3 0 114 227 112 887.3 0 112 228 76 887.3 0 76 229 86 887.3 0 86 230 92 887.3 0 92 231 81 887.3 0 81 232 62 887.3 0 62 233 77 887.3 0 77 234 84 887.3 0 84 235 94 887.3 0 94 236 101 887.3 0 101 237 103 887.3 0 103 238 179 887.3 0 179 239 216 887.3 0 216 240 146 887.3 0 146 241 164 887.3 0 164 242 102 887.3 0 102 243 132 887.3 0 132 244 119 887.3 0 119 245 154 887.3 0 154 246 141 887.3 0 141 247 143 887.3 0 143 248 161 887.3 0 161 249 119 887.3 0 119 250 86 887.3 0 86 251 95 887.3 0 95 252 113 887.3 0 113 253 101 887.3 0 101 254 112 887.3 0 112 255 135 887.3 0 135 256 390 887.3 0 390 257 433 887.3 0 433 258 541 887.3 0 541 259 182 887.3 0 182 260 139 887.3 0 139 261 114 887.3 0 114 262 98 887.3 0 98 263 90 887.3 0 90 264 106 887.3 0 106 265 90 887.3 0 90 266 254 887.3 0 254 267 383 887.3 0 383 268 530 887.3 0 530 269 206 887.3 0 206 270 170 887.3 0 170 271 195 887.3 0 195 272 633 887.3 0 633 273 693 887.3 0 693 274 546 887.3 0 546 275 308 887.3 0 308 276 206 887.3 0 206 277 210 887.3 0 210 278 201 887.3 0 201 279 136 887.3 0 136 280 241 887.3 0 241 281 186 887.3 0 186 282 232 887.3 0 232 283 168 887.3 0 168 284 139 887.3 0 139 285 147 887.3 0 147 286 161 887.3 0 161 287 318 887.3 0 318 288 265 887.3 0 265 289 253 887.3 0 253 290 114 887.3 0 114 291 105 887.3 0 105 292 128 887.3 0 128 293 182 887.3 0 182 294 186 887.3 0 186 295 99 887.3 0 99 296 66 887.3 0 66 297 96 887.3 0 96 298 121 887.3 0 121 299 126 887.3 0 126 300 136 887.3 0 136 301 173 887.3 0 173 Finished in 6.17 s (70 us/read; 0.86 M reads/minute). === Summary === Total reads processed: 88,200 Reads with adapters: 19,670 (22.3%) Reads that were too short: 25 (0.0%) Reads written (passing filters): 19,645 (22.3%) Total basepairs processed: 18,644,807 bp Total written (filtered): 5,363,932 bp (28.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 19670 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 67 0.0 3 0 0 4 0 63 19 295 0.0 3 0 281 11 0 3 20 18744 0.0 4 18423 308 11 1 1 21 424 0.0 4 383 40 1 22 6 0.0 4 2 0 4 23 14 0.0 4 1 0 0 10 3 29 2 0.0 4 1 0 0 0 1 56 1 0.0 4 0 0 0 0 1 60 2 0.0 4 0 0 0 0 2 61 2 0.0 4 0 0 0 1 1 75 1 0.0 4 1 94 1 0.0 4 0 0 0 0 1 100 2 0.0 4 0 0 1 1 101 2 0.0 4 0 0 1 1 102 1 0.0 4 1 105 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 127 87 0.0 4 0 0 0 0 87 128 1 0.0 4 1 130 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 1 2 229 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 252 2 0.0 4 0 0 0 0 2 255 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.14 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 19,645 Reads with adapters: 385 (2.0%) Reads written (passing filters): 19,645 (100.0%) Total basepairs processed: 5,363,932 bp Total written (filtered): 5,359,893 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 385 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 8.3% C: 4.7% G: 84.2% T: 2.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 37 307.0 0 37 4 3 76.7 0 3 5 2 19.2 0 2 6 1 4.8 0 1 10 6 0.0 1 0 6 11 320 0.0 1 1 319 12 15 0.0 1 1 14 140 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.