This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGATCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 151.35 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 614,140 (19.4%) Reads that were too short: 9,015 (0.3%) Reads written (passing filters): 605,125 (19.1%) Total basepairs processed: 953,096,634 bp Total written (filtered): 153,126,375 bp (16.1%) === Adapter 1 === Sequence: TGATCC; Type: regular 5'; Length: 6; Trimmed: 614140 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2168 773.1 0 2168 7 10400 773.1 0 10400 8 10683 773.1 0 10683 9 74742 773.1 0 74742 10 184 773.1 0 184 11 228 773.1 0 228 12 156 773.1 0 156 13 23 773.1 0 23 14 31 773.1 0 31 15 55 773.1 0 55 16 74 773.1 0 74 17 86 773.1 0 86 18 33 773.1 0 33 19 66 773.1 0 66 20 55 773.1 0 55 21 102 773.1 0 102 22 242 773.1 0 242 23 87 773.1 0 87 24 164 773.1 0 164 25 149 773.1 0 149 26 151 773.1 0 151 27 453 773.1 0 453 28 125 773.1 0 125 29 242 773.1 0 242 30 213 773.1 0 213 31 253 773.1 0 253 32 161 773.1 0 161 33 363 773.1 0 363 34 424 773.1 0 424 35 844 773.1 0 844 36 676 773.1 0 676 37 1416 773.1 0 1416 38 2754 773.1 0 2754 39 41086 773.1 0 41086 40 26814 773.1 0 26814 41 64052 773.1 0 64052 42 469 773.1 0 469 43 349 773.1 0 349 44 531 773.1 0 531 45 1096 773.1 0 1096 46 2213 773.1 0 2213 47 477 773.1 0 477 48 550 773.1 0 550 49 997 773.1 0 997 50 374 773.1 0 374 51 438 773.1 0 438 52 1003 773.1 0 1003 53 811 773.1 0 811 54 1176 773.1 0 1176 55 1221 773.1 0 1221 56 1984 773.1 0 1984 57 2796 773.1 0 2796 58 3917 773.1 0 3917 59 107869 773.1 0 107869 60 82558 773.1 0 82558 61 144202 773.1 0 144202 62 1319 773.1 0 1319 63 20 773.1 0 20 64 15 773.1 0 15 65 4 773.1 0 4 66 3 773.1 0 3 67 6 773.1 0 6 68 9 773.1 0 9 69 4 773.1 0 4 70 10 773.1 0 10 71 14 773.1 0 14 72 32 773.1 0 32 73 97 773.1 0 97 74 97 773.1 0 97 75 90 773.1 0 90 76 3 773.1 0 3 77 5 773.1 0 5 78 7 773.1 0 7 79 7 773.1 0 7 80 7 773.1 0 7 81 36 773.1 0 36 82 33 773.1 0 33 83 29 773.1 0 29 84 6 773.1 0 6 85 7 773.1 0 7 86 12 773.1 0 12 87 6 773.1 0 6 88 11 773.1 0 11 89 9 773.1 0 9 90 6 773.1 0 6 91 7 773.1 0 7 92 6 773.1 0 6 93 4 773.1 0 4 94 5 773.1 0 5 95 7 773.1 0 7 96 5 773.1 0 5 97 6 773.1 0 6 98 13 773.1 0 13 99 12 773.1 0 12 100 28 773.1 0 28 101 415 773.1 0 415 102 589 773.1 0 589 103 511 773.1 0 511 104 8 773.1 0 8 105 16 773.1 0 16 106 21 773.1 0 21 107 5 773.1 0 5 108 13 773.1 0 13 109 26 773.1 0 26 110 71 773.1 0 71 111 1306 773.1 0 1306 112 311 773.1 0 311 113 545 773.1 0 545 114 10 773.1 0 10 115 2 773.1 0 2 116 3 773.1 0 3 117 1 773.1 0 1 118 11 773.1 0 11 119 14 773.1 0 14 120 6 773.1 0 6 121 4 773.1 0 4 122 5 773.1 0 5 123 11 773.1 0 11 124 4 773.1 0 4 125 31 773.1 0 31 126 20 773.1 0 20 127 8 773.1 0 8 128 26 773.1 0 26 129 10 773.1 0 10 130 20 773.1 0 20 131 42 773.1 0 42 132 14 773.1 0 14 133 4 773.1 0 4 134 7 773.1 0 7 135 8 773.1 0 8 136 10 773.1 0 10 137 15 773.1 0 15 138 13 773.1 0 13 139 16 773.1 0 16 140 11 773.1 0 11 141 25 773.1 0 25 142 15 773.1 0 15 143 20 773.1 0 20 144 13 773.1 0 13 145 13 773.1 0 13 146 37 773.1 0 37 147 17 773.1 0 17 148 26 773.1 0 26 149 30 773.1 0 30 150 16 773.1 0 16 151 9 773.1 0 9 152 10 773.1 0 10 153 5 773.1 0 5 154 5 773.1 0 5 155 10 773.1 0 10 156 15 773.1 0 15 157 56 773.1 0 56 158 13 773.1 0 13 159 9 773.1 0 9 160 12 773.1 0 12 161 17 773.1 0 17 162 56 773.1 0 56 163 36 773.1 0 36 164 21 773.1 0 21 165 10 773.1 0 10 166 14 773.1 0 14 167 45 773.1 0 45 168 24 773.1 0 24 169 62 773.1 0 62 170 92 773.1 0 92 171 104 773.1 0 104 172 14 773.1 0 14 173 14 773.1 0 14 174 12 773.1 0 12 175 6 773.1 0 6 176 7 773.1 0 7 177 20 773.1 0 20 178 38 773.1 0 38 179 22 773.1 0 22 180 13 773.1 0 13 181 21 773.1 0 21 182 19 773.1 0 19 183 80 773.1 0 80 184 321 773.1 0 321 185 126 773.1 0 126 186 206 773.1 0 206 187 82 773.1 0 82 188 43 773.1 0 43 189 40 773.1 0 40 190 22 773.1 0 22 191 10 773.1 0 10 192 8 773.1 0 8 193 15 773.1 0 15 194 13 773.1 0 13 195 33 773.1 0 33 196 50 773.1 0 50 197 252 773.1 0 252 198 671 773.1 0 671 199 392 773.1 0 392 200 646 773.1 0 646 201 146 773.1 0 146 202 115 773.1 0 115 203 303 773.1 0 303 204 209 773.1 0 209 205 526 773.1 0 526 206 43 773.1 0 43 207 23 773.1 0 23 208 22 773.1 0 22 209 22 773.1 0 22 210 24 773.1 0 24 211 16 773.1 0 16 212 22 773.1 0 22 213 17 773.1 0 17 214 27 773.1 0 27 215 21 773.1 0 21 216 13 773.1 0 13 217 9 773.1 0 9 218 74 773.1 0 74 219 79 773.1 0 79 220 105 773.1 0 105 221 43 773.1 0 43 222 24 773.1 0 24 223 320 773.1 0 320 224 424 773.1 0 424 225 400 773.1 0 400 226 386 773.1 0 386 227 445 773.1 0 445 228 1136 773.1 0 1136 229 486 773.1 0 486 230 494 773.1 0 494 231 8 773.1 0 8 232 7 773.1 0 7 233 6 773.1 0 6 234 6 773.1 0 6 235 15 773.1 0 15 236 15 773.1 0 15 237 13 773.1 0 13 238 19 773.1 0 19 239 21 773.1 0 21 240 12 773.1 0 12 241 18 773.1 0 18 242 21 773.1 0 21 243 19 773.1 0 19 244 32 773.1 0 32 245 17 773.1 0 17 246 12 773.1 0 12 247 17 773.1 0 17 248 26 773.1 0 26 249 19 773.1 0 19 250 16 773.1 0 16 251 19 773.1 0 19 252 5 773.1 0 5 253 7 773.1 0 7 254 14 773.1 0 14 255 90 773.1 0 90 256 112 773.1 0 112 257 153 773.1 0 153 258 117 773.1 0 117 259 90 773.1 0 90 260 51 773.1 0 51 261 49 773.1 0 49 262 17 773.1 0 17 263 24 773.1 0 24 264 29 773.1 0 29 265 15 773.1 0 15 266 19 773.1 0 19 267 18 773.1 0 18 268 31 773.1 0 31 269 17 773.1 0 17 270 21 773.1 0 21 271 61 773.1 0 61 272 58 773.1 0 58 273 94 773.1 0 94 274 97 773.1 0 97 275 171 773.1 0 171 276 88 773.1 0 88 277 158 773.1 0 158 278 19 773.1 0 19 279 40 773.1 0 40 280 26 773.1 0 26 281 49 773.1 0 49 282 22 773.1 0 22 283 23 773.1 0 23 284 29 773.1 0 29 285 65 773.1 0 65 286 68 773.1 0 68 287 65 773.1 0 65 288 37 773.1 0 37 289 38 773.1 0 38 290 67 773.1 0 67 291 93 773.1 0 93 292 129 773.1 0 129 293 106 773.1 0 106 294 76 773.1 0 76 295 37 773.1 0 37 296 32 773.1 0 32 297 33 773.1 0 33 298 30 773.1 0 30 299 41 773.1 0 41 300 44 773.1 0 44 301 74 773.1 0 74 Finished in 35.30 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 605,125 Reads with adapters: 14,552 (2.4%) Reads that were too short: 764 (0.1%) Reads written (passing filters): 13,788 (2.3%) Total basepairs processed: 153,126,375 bp Total written (filtered): 3,338,036 bp (2.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 14552 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 5 0.0 3 0 0 3 2 16 83 0.0 3 0 66 11 6 17 10566 0.0 3 10058 440 50 18 18 31 0.0 3 1 25 2 3 19 19 0.0 3 1 1 8 9 20 28 0.0 3 0 0 0 28 23 1 0.0 3 0 1 24 22 0.0 3 0 0 1 21 26 2 0.0 3 0 0 0 2 29 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 41 15 0.0 3 0 0 0 15 43 5 0.0 3 0 0 0 5 44 1 0.0 3 0 0 0 1 47 4 0.0 3 0 0 0 4 48 5 0.0 3 3 1 1 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 61 3 0.0 3 0 0 0 3 62 2 0.0 3 0 0 0 2 63 9 0.0 3 0 0 0 9 64 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 68 2 0.0 3 0 0 0 2 69 1 0.0 3 0 0 0 1 74 2 0.0 3 1 0 1 75 3 0.0 3 0 0 0 3 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 81 46 0.0 3 0 0 1 45 83 1 0.0 3 0 0 0 1 84 8 0.0 3 0 0 0 8 86 2 0.0 3 1 0 0 1 88 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 90 2 0.0 3 0 0 0 2 91 1 0.0 3 0 0 0 1 92 6 0.0 3 0 0 0 6 94 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 98 1 0.0 3 1 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 102 2 0.0 3 0 0 0 2 103 3 0.0 3 1 0 0 2 106 1 0.0 3 0 0 0 1 107 20 0.0 3 0 0 0 20 109 3 0.0 3 0 0 0 3 110 9 0.0 3 0 0 2 7 111 1 0.0 3 0 0 0 1 112 53 0.0 3 0 0 1 52 113 4 0.0 3 0 0 0 4 114 32 0.0 3 0 0 1 31 115 11 0.0 3 0 0 1 10 119 1 0.0 3 1 121 2 0.0 3 0 0 0 2 122 5 0.0 3 0 0 0 5 123 3 0.0 3 0 0 0 3 124 2 0.0 3 0 0 0 2 125 2 0.0 3 0 0 0 2 126 7 0.0 3 0 0 0 7 127 15 0.0 3 0 0 0 15 128 6 0.0 3 0 0 0 6 129 2 0.0 3 0 0 0 2 130 9 0.0 3 0 0 1 8 131 34 0.0 3 0 0 1 33 132 1072 0.0 3 0 0 5 1067 133 81 0.0 3 0 0 0 81 134 3 0.0 3 0 0 0 3 135 170 0.0 3 0 0 5 165 136 18 0.0 3 0 0 0 18 138 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 143 2 0.0 3 0 0 0 2 144 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 148 3 0.0 3 0 0 1 2 149 1 0.0 3 0 0 0 1 150 2 0.0 3 0 0 0 2 151 7 0.0 3 1 0 0 6 152 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 155 2 0.0 3 0 0 0 2 156 8 0.0 3 0 0 0 8 157 3 0.0 3 0 0 0 3 158 82 0.0 3 0 0 3 79 159 6 0.0 3 0 0 0 6 160 31 0.0 3 0 0 0 31 161 9 0.0 3 0 0 0 9 162 9 0.0 3 0 0 0 9 163 633 0.0 3 0 0 10 623 164 71 0.0 3 0 0 0 71 165 6 0.0 3 0 0 0 6 166 80 0.0 3 0 0 4 76 167 12 0.0 3 0 0 0 12 168 4 0.0 3 0 0 0 4 169 1 0.0 3 0 0 0 1 170 2 0.0 3 0 0 0 2 171 5 0.0 3 0 0 0 5 172 2 0.0 3 0 0 0 2 173 3 0.0 3 0 0 0 3 175 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 177 78 0.0 3 0 0 2 76 178 11 0.0 3 0 0 0 11 179 1 0.0 3 0 0 0 1 180 17 0.0 3 0 0 1 16 181 1 0.0 3 0 0 0 1 182 8 0.0 3 0 0 0 8 183 251 0.0 3 0 0 4 247 184 119 0.0 3 0 0 1 118 185 8 0.0 3 0 0 0 8 186 25 0.0 3 0 0 0 25 187 7 0.0 3 0 0 0 7 188 6 0.0 3 0 0 0 6 189 6 0.0 3 0 0 0 6 190 3 0.0 3 0 0 0 3 191 2 0.0 3 0 0 0 2 192 14 0.0 3 0 0 0 14 193 2 0.0 3 0 0 0 2 194 2 0.0 3 0 0 0 2 195 12 0.0 3 0 0 0 12 196 1 0.0 3 0 0 1 197 2 0.0 3 0 0 0 2 199 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 204 3 0.0 3 0 0 0 3 206 4 0.0 3 0 0 0 4 207 6 0.0 3 0 0 0 6 208 1 0.0 3 0 0 0 1 209 3 0.0 3 0 0 0 3 211 3 0.0 3 0 0 0 3 212 1 0.0 3 0 0 0 1 213 2 0.0 3 0 0 0 2 214 6 0.0 3 0 0 0 6 215 3 0.0 3 0 0 0 3 216 4 0.0 3 0 0 2 2 217 1 0.0 3 0 0 0 1 218 4 0.0 3 0 0 0 4 219 2 0.0 3 0 0 0 2 220 1 0.0 3 0 0 0 1 221 3 0.0 3 0 0 0 3 223 5 0.0 3 0 0 0 5 224 9 0.0 3 0 0 1 8 225 1 0.0 3 0 0 0 1 230 2 0.0 3 0 0 0 2 231 1 0.0 3 0 0 0 1 232 3 0.0 3 0 0 0 3 233 67 0.0 3 0 0 2 65 234 194 0.0 3 0 0 6 188 235 16 0.0 3 0 0 2 14 236 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 239 3 0.0 3 0 0 0 3 241 2 0.0 3 0 0 0 2 242 14 0.0 3 0 0 2 12 243 2 0.0 3 0 0 0 2 244 6 0.0 3 0 0 0 6 247 11 0.0 3 0 0 0 11 248 3 0.0 3 0 0 0 3 249 14 0.0 3 0 0 1 13 250 11 0.0 3 0 0 1 10 251 1 0.0 3 0 0 0 1 252 2 0.0 3 0 0 0 2 253 2 0.0 3 0 0 0 2 255 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 0 1 258 2 0.0 3 0 0 0 2 259 1 0.0 3 0 0 0 1 260 2 0.0 3 0 0 0 2 263 2 0.0 3 0 0 0 2 264 1 0.0 3 0 0 0 1 265 1 0.0 3 0 0 0 1 266 1 0.0 3 0 0 0 1 275 8 0.0 3 0 0 0 8 276 5 0.0 3 0 0 0 5 277 5 0.0 3 0 0 0 5 278 8 0.0 3 0 0 0 8 279 6 0.0 3 0 0 0 6 280 2 0.0 3 0 0 0 2 281 1 0.0 3 0 0 0 1 283 2 0.0 3 0 0 0 2 284 18 0.0 3 0 0 1 17 285 5 0.0 3 0 0 0 5 287 1 0.0 3 0 0 0 1 288 2 0.0 3 0 0 0 2 289 5 0.0 3 0 0 1 4 290 2 0.0 3 0 0 0 2 291 1 0.0 3 0 0 0 1 292 9 0.0 3 0 0 0 9 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.70 s (51 us/read; 1.18 M reads/minute). === Summary === Total reads processed: 13,788 Reads with adapters: 49 (0.4%) Reads written (passing filters): 13,788 (100.0%) Total basepairs processed: 3,338,036 bp Total written (filtered): 3,337,884 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 49 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 36.7% C: 30.6% G: 18.4% T: 14.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 45 215.4 0 45 4 3 53.9 0 3 5 1 13.5 0 1