This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGATCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 150.54 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 635,421 (20.1%) Reads that were too short: 4,825 (0.2%) Reads written (passing filters): 630,596 (19.9%) Total basepairs processed: 953,096,634 bp Total written (filtered): 159,830,257 bp (16.8%) === Adapter 1 === Sequence: TGATCC; Type: regular 5'; Length: 6; Trimmed: 635421 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1357 773.1 0 1357 7 9609 773.1 0 9609 8 12336 773.1 0 12336 9 54110 773.1 0 54110 10 121 773.1 0 121 11 104 773.1 0 104 12 207 773.1 0 207 13 19 773.1 0 19 14 27 773.1 0 27 15 65 773.1 0 65 16 55 773.1 0 55 17 93 773.1 0 93 18 55 773.1 0 55 19 70 773.1 0 70 20 43 773.1 0 43 21 139 773.1 0 139 22 339 773.1 0 339 23 90 773.1 0 90 24 194 773.1 0 194 25 185 773.1 0 185 26 200 773.1 0 200 27 691 773.1 0 691 28 179 773.1 0 179 29 213 773.1 0 213 30 128 773.1 0 128 31 171 773.1 0 171 32 229 773.1 0 229 33 374 773.1 0 374 34 522 773.1 0 522 35 1017 773.1 0 1017 36 834 773.1 0 834 37 1879 773.1 0 1879 38 3476 773.1 0 3476 39 62444 773.1 0 62444 40 38687 773.1 0 38687 41 96791 773.1 0 96791 42 581 773.1 0 581 43 373 773.1 0 373 44 558 773.1 0 558 45 1030 773.1 0 1030 46 2216 773.1 0 2216 47 470 773.1 0 470 48 478 773.1 0 478 49 995 773.1 0 995 50 358 773.1 0 358 51 424 773.1 0 424 52 940 773.1 0 940 53 752 773.1 0 752 54 943 773.1 0 943 55 1005 773.1 0 1005 56 1670 773.1 0 1670 57 2376 773.1 0 2376 58 2815 773.1 0 2815 59 100415 773.1 0 100415 60 79264 773.1 0 79264 61 138554 773.1 0 138554 62 1158 773.1 0 1158 63 22 773.1 0 22 64 8 773.1 0 8 65 5 773.1 0 5 66 3 773.1 0 3 68 3 773.1 0 3 69 3 773.1 0 3 70 4 773.1 0 4 71 10 773.1 0 10 72 24 773.1 0 24 73 120 773.1 0 120 74 79 773.1 0 79 75 132 773.1 0 132 76 4 773.1 0 4 77 3 773.1 0 3 78 1 773.1 0 1 79 1 773.1 0 1 80 9 773.1 0 9 81 16 773.1 0 16 82 12 773.1 0 12 83 5 773.1 0 5 84 4 773.1 0 4 85 2 773.1 0 2 86 1 773.1 0 1 87 3 773.1 0 3 88 4 773.1 0 4 89 5 773.1 0 5 90 5 773.1 0 5 91 3 773.1 0 3 92 2 773.1 0 2 93 2 773.1 0 2 94 2 773.1 0 2 95 5 773.1 0 5 96 3 773.1 0 3 97 8 773.1 0 8 98 8 773.1 0 8 99 5 773.1 0 5 100 21 773.1 0 21 101 194 773.1 0 194 102 300 773.1 0 300 103 342 773.1 0 342 104 8 773.1 0 8 105 9 773.1 0 9 106 12 773.1 0 12 107 6 773.1 0 6 108 10 773.1 0 10 109 9 773.1 0 9 110 43 773.1 0 43 111 1137 773.1 0 1137 112 262 773.1 0 262 113 509 773.1 0 509 114 6 773.1 0 6 115 2 773.1 0 2 116 3 773.1 0 3 117 7 773.1 0 7 118 7 773.1 0 7 119 6 773.1 0 6 120 2 773.1 0 2 121 3 773.1 0 3 122 6 773.1 0 6 123 3 773.1 0 3 124 4 773.1 0 4 125 27 773.1 0 27 126 19 773.1 0 19 127 11 773.1 0 11 128 24 773.1 0 24 129 7 773.1 0 7 130 16 773.1 0 16 131 33 773.1 0 33 132 7 773.1 0 7 133 4 773.1 0 4 134 6 773.1 0 6 135 4 773.1 0 4 136 5 773.1 0 5 137 8 773.1 0 8 138 5 773.1 0 5 139 8 773.1 0 8 140 3 773.1 0 3 141 20 773.1 0 20 142 6 773.1 0 6 143 14 773.1 0 14 144 4 773.1 0 4 145 10 773.1 0 10 146 42 773.1 0 42 147 16 773.1 0 16 148 34 773.1 0 34 149 18 773.1 0 18 150 18 773.1 0 18 151 2 773.1 0 2 152 6 773.1 0 6 153 7 773.1 0 7 154 4 773.1 0 4 155 6 773.1 0 6 156 15 773.1 0 15 157 55 773.1 0 55 158 11 773.1 0 11 159 7 773.1 0 7 160 8 773.1 0 8 161 7 773.1 0 7 162 33 773.1 0 33 163 14 773.1 0 14 164 12 773.1 0 12 165 1 773.1 0 1 166 17 773.1 0 17 167 46 773.1 0 46 168 13 773.1 0 13 169 79 773.1 0 79 170 102 773.1 0 102 171 81 773.1 0 81 172 4 773.1 0 4 173 9 773.1 0 9 174 5 773.1 0 5 175 3 773.1 0 3 176 3 773.1 0 3 177 10 773.1 0 10 178 16 773.1 0 16 179 10 773.1 0 10 180 6 773.1 0 6 181 13 773.1 0 13 182 16 773.1 0 16 183 54 773.1 0 54 184 357 773.1 0 357 185 57 773.1 0 57 186 213 773.1 0 213 187 67 773.1 0 67 188 22 773.1 0 22 189 21 773.1 0 21 190 5 773.1 0 5 191 10 773.1 0 10 192 6 773.1 0 6 193 11 773.1 0 11 194 15 773.1 0 15 195 10 773.1 0 10 196 28 773.1 0 28 197 126 773.1 0 126 198 272 773.1 0 272 199 165 773.1 0 165 200 259 773.1 0 259 201 43 773.1 0 43 202 27 773.1 0 27 203 60 773.1 0 60 204 28 773.1 0 28 205 75 773.1 0 75 206 14 773.1 0 14 207 10 773.1 0 10 208 6 773.1 0 6 209 6 773.1 0 6 210 3 773.1 0 3 211 8 773.1 0 8 212 7 773.1 0 7 213 9 773.1 0 9 214 11 773.1 0 11 215 10 773.1 0 10 216 10 773.1 0 10 217 7 773.1 0 7 218 26 773.1 0 26 219 19 773.1 0 19 220 33 773.1 0 33 221 25 773.1 0 25 222 21 773.1 0 21 223 75 773.1 0 75 224 114 773.1 0 114 225 111 773.1 0 111 226 373 773.1 0 373 227 451 773.1 0 451 228 1056 773.1 0 1056 229 441 773.1 0 441 230 245 773.1 0 245 231 4 773.1 0 4 232 5 773.1 0 5 233 3 773.1 0 3 234 5 773.1 0 5 235 12 773.1 0 12 236 11 773.1 0 11 237 5 773.1 0 5 238 14 773.1 0 14 239 8 773.1 0 8 240 4 773.1 0 4 241 13 773.1 0 13 242 5 773.1 0 5 243 17 773.1 0 17 244 6 773.1 0 6 245 10 773.1 0 10 246 5 773.1 0 5 247 7 773.1 0 7 248 11 773.1 0 11 249 11 773.1 0 11 250 11 773.1 0 11 251 6 773.1 0 6 252 3 773.1 0 3 253 7 773.1 0 7 254 7 773.1 0 7 255 37 773.1 0 37 256 47 773.1 0 47 257 79 773.1 0 79 258 40 773.1 0 40 259 33 773.1 0 33 260 16 773.1 0 16 261 13 773.1 0 13 262 8 773.1 0 8 263 12 773.1 0 12 264 15 773.1 0 15 265 15 773.1 0 15 266 8 773.1 0 8 267 11 773.1 0 11 268 11 773.1 0 11 269 6 773.1 0 6 270 13 773.1 0 13 271 24 773.1 0 24 272 34 773.1 0 34 273 60 773.1 0 60 274 53 773.1 0 53 275 146 773.1 0 146 276 54 773.1 0 54 277 130 773.1 0 130 278 8 773.1 0 8 279 17 773.1 0 17 280 18 773.1 0 18 281 16 773.1 0 16 282 5 773.1 0 5 283 11 773.1 0 11 284 7 773.1 0 7 285 32 773.1 0 32 286 30 773.1 0 30 287 34 773.1 0 34 288 15 773.1 0 15 289 14 773.1 0 14 290 22 773.1 0 22 291 34 773.1 0 34 292 66 773.1 0 66 293 31 773.1 0 31 294 30 773.1 0 30 295 11 773.1 0 11 296 10 773.1 0 10 297 12 773.1 0 12 298 15 773.1 0 15 299 16 773.1 0 16 300 13 773.1 0 13 301 22 773.1 0 22 Finished in 41.22 s (65 us/read; 0.92 M reads/minute). === Summary === Total reads processed: 630,596 Reads with adapters: 30,121 (4.8%) Reads that were too short: 118 (0.0%) Reads written (passing filters): 30,003 (4.8%) Total basepairs processed: 159,830,257 bp Total written (filtered): 6,333,786 bp (4.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 30121 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 2 0.0 3 0 0 2 19 51 0.0 3 0 50 1 20 11866 0.0 4 11682 175 7 1 1 21 31 0.0 4 1 29 1 22 12 0.0 4 0 11 1 23 621 0.0 4 604 14 0 3 24 2 0.0 4 1 1 28 1 0.0 4 0 0 0 0 1 40 2 0.0 4 0 0 0 0 2 43 1 0.0 4 0 0 0 0 1 45 1 0.0 4 1 72 2 0.0 4 0 0 0 0 2 73 20 0.0 4 0 0 0 0 20 74 16 0.0 4 0 0 0 0 16 75 16331 0.0 4 0 0 0 0 16331 76 1 0.0 4 0 0 0 0 1 77 2 0.0 4 0 0 0 0 2 78 5 0.0 4 0 0 0 0 5 83 3 0.0 4 0 0 0 0 3 85 1 0.0 4 0 0 0 0 1 87 1 0.0 4 1 105 1 0.0 4 1 107 1 0.0 4 1 113 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 71 0.0 4 0 0 0 0 71 127 948 0.0 4 0 0 0 0 948 128 6 0.0 4 0 0 0 0 6 151 1 0.0 4 0 0 0 0 1 155 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 158 3 0.0 4 0 0 0 0 3 164 1 0.0 4 0 0 0 0 1 171 2 0.0 4 0 0 0 0 2 173 1 0.0 4 0 0 0 0 1 176 36 0.0 4 0 0 0 0 36 177 12 0.0 4 0 0 0 2 10 178 2 0.0 4 0 0 0 0 2 181 2 0.0 4 0 0 0 0 2 182 4 0.0 4 0 0 0 0 4 183 5 0.0 4 0 0 0 0 5 184 5 0.0 4 0 0 0 0 5 186 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 212 1 0.0 4 0 0 0 0 1 220 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 230 16 0.0 4 0 0 0 0 16 231 9 0.0 4 0 0 0 0 9 232 2 0.0 4 0 0 0 0 2 233 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 261 1 0.0 4 0 0 0 0 1 272 1 0.0 4 1 281 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.45 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 30,003 Reads with adapters: 1,286 (4.3%) Reads written (passing filters): 30,003 (100.0%) Total basepairs processed: 6,333,786 bp Total written (filtered): 6,319,544 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1286 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 13.1% C: 3.2% G: 82.7% T: 1.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 46 468.8 0 46 4 123 117.2 0 123 9 53 0.1 0 2 51 10 85 0.0 1 4 81 11 969 0.0 1 9 960 12 1 0.0 1 0 1 13 1 0.0 1 0 1 56 1 0.0 1 1 58 1 0.0 1 1 209 1 0.0 1 0 1 224 1 0.0 1 1 259 1 0.0 1 1 265 3 0.0 1 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.