This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CAGAGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 141.53 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 164,335 (5.2%) Reads that were too short: 31,125 (1.0%) Reads written (passing filters): 133,210 (4.2%) Total basepairs processed: 953,096,634 bp Total written (filtered): 37,317,129 bp (3.9%) === Adapter 1 === Sequence: CAGAGC; Type: regular 5'; Length: 6; Trimmed: 164335 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3607 773.1 0 3607 7 53740 773.1 0 53740 8 892 773.1 0 892 9 57998 773.1 0 57998 10 62 773.1 0 62 11 110 773.1 0 110 12 53 773.1 0 53 13 5 773.1 0 5 14 4 773.1 0 4 15 4 773.1 0 4 16 3 773.1 0 3 17 22 773.1 0 22 18 6 773.1 0 6 19 5 773.1 0 5 20 5 773.1 0 5 21 3 773.1 0 3 22 11 773.1 0 11 23 6 773.1 0 6 24 12 773.1 0 12 25 10 773.1 0 10 26 12 773.1 0 12 27 55 773.1 0 55 28 383 773.1 0 383 29 72 773.1 0 72 30 10 773.1 0 10 31 10 773.1 0 10 32 9 773.1 0 9 33 9 773.1 0 9 34 21 773.1 0 21 35 10 773.1 0 10 36 9 773.1 0 9 37 25 773.1 0 25 38 12 773.1 0 12 39 15 773.1 0 15 40 4 773.1 0 4 41 10 773.1 0 10 42 19 773.1 0 19 43 15 773.1 0 15 44 10 773.1 0 10 45 29 773.1 0 29 46 28 773.1 0 28 47 32 773.1 0 32 48 154 773.1 0 154 49 8 773.1 0 8 50 46 773.1 0 46 51 213 773.1 0 213 52 35 773.1 0 35 53 29 773.1 0 29 54 28 773.1 0 28 55 41 773.1 0 41 56 20 773.1 0 20 57 8 773.1 0 8 58 21 773.1 0 21 59 59 773.1 0 59 60 38 773.1 0 38 61 144 773.1 0 144 62 556 773.1 0 556 63 524 773.1 0 524 64 486 773.1 0 486 65 68 773.1 0 68 66 56 773.1 0 56 67 29 773.1 0 29 68 48 773.1 0 48 69 41 773.1 0 41 70 16 773.1 0 16 71 18 773.1 0 18 72 56 773.1 0 56 73 84 773.1 0 84 74 53 773.1 0 53 75 66 773.1 0 66 76 68 773.1 0 68 77 101 773.1 0 101 78 1214 773.1 0 1214 79 979 773.1 0 979 80 1416 773.1 0 1416 81 353 773.1 0 353 82 115 773.1 0 115 83 831 773.1 0 831 84 36 773.1 0 36 85 22 773.1 0 22 86 160 773.1 0 160 87 16 773.1 0 16 88 11 773.1 0 11 89 18 773.1 0 18 90 22 773.1 0 22 91 22 773.1 0 22 92 29 773.1 0 29 93 31 773.1 0 31 94 26 773.1 0 26 95 22 773.1 0 22 96 18 773.1 0 18 97 16 773.1 0 16 98 10 773.1 0 10 99 15 773.1 0 15 100 27 773.1 0 27 101 29 773.1 0 29 102 124 773.1 0 124 103 20 773.1 0 20 104 8 773.1 0 8 105 19 773.1 0 19 106 8 773.1 0 8 107 18 773.1 0 18 108 38 773.1 0 38 109 115 773.1 0 115 110 92 773.1 0 92 111 94 773.1 0 94 112 21 773.1 0 21 113 20 773.1 0 20 114 23 773.1 0 23 115 73 773.1 0 73 116 131 773.1 0 131 117 120 773.1 0 120 118 40 773.1 0 40 119 35 773.1 0 35 120 26 773.1 0 26 121 52 773.1 0 52 122 48 773.1 0 48 123 57 773.1 0 57 124 62 773.1 0 62 125 92 773.1 0 92 126 94 773.1 0 94 127 52 773.1 0 52 128 42 773.1 0 42 129 43 773.1 0 43 130 66 773.1 0 66 131 58 773.1 0 58 132 35 773.1 0 35 133 28 773.1 0 28 134 12 773.1 0 12 135 20 773.1 0 20 136 20 773.1 0 20 137 30 773.1 0 30 138 34 773.1 0 34 139 18 773.1 0 18 140 22 773.1 0 22 141 79 773.1 0 79 142 87 773.1 0 87 143 44 773.1 0 44 144 60 773.1 0 60 145 54 773.1 0 54 146 92 773.1 0 92 147 26 773.1 0 26 148 47 773.1 0 47 149 20 773.1 0 20 150 82 773.1 0 82 151 35 773.1 0 35 152 33 773.1 0 33 153 26 773.1 0 26 154 39 773.1 0 39 155 33 773.1 0 33 156 27 773.1 0 27 157 83 773.1 0 83 158 31 773.1 0 31 159 30 773.1 0 30 160 44 773.1 0 44 161 27 773.1 0 27 162 36 773.1 0 36 163 28 773.1 0 28 164 38 773.1 0 38 165 66 773.1 0 66 166 71 773.1 0 71 167 80 773.1 0 80 168 46 773.1 0 46 169 25 773.1 0 25 170 36 773.1 0 36 171 55 773.1 0 55 172 55 773.1 0 55 173 34 773.1 0 34 174 35 773.1 0 35 175 36 773.1 0 36 176 49 773.1 0 49 177 43 773.1 0 43 178 41 773.1 0 41 179 62 773.1 0 62 180 100 773.1 0 100 181 394 773.1 0 394 182 343 773.1 0 343 183 423 773.1 0 423 184 110 773.1 0 110 185 82 773.1 0 82 186 335 773.1 0 335 187 234 773.1 0 234 188 156 773.1 0 156 189 31 773.1 0 31 190 201 773.1 0 201 191 187 773.1 0 187 192 245 773.1 0 245 193 137 773.1 0 137 194 77 773.1 0 77 195 47 773.1 0 47 196 92 773.1 0 92 197 79 773.1 0 79 198 117 773.1 0 117 199 89 773.1 0 89 200 41 773.1 0 41 201 36 773.1 0 36 202 38 773.1 0 38 203 62 773.1 0 62 204 126 773.1 0 126 205 107 773.1 0 107 206 79 773.1 0 79 207 36 773.1 0 36 208 107 773.1 0 107 209 138 773.1 0 138 210 328 773.1 0 328 211 186 773.1 0 186 212 99 773.1 0 99 213 89 773.1 0 89 214 87 773.1 0 87 215 115 773.1 0 115 216 109 773.1 0 109 217 573 773.1 0 573 218 638 773.1 0 638 219 632 773.1 0 632 220 126 773.1 0 126 221 128 773.1 0 128 222 198 773.1 0 198 223 177 773.1 0 177 224 206 773.1 0 206 225 3084 773.1 0 3084 226 2662 773.1 0 2662 227 5081 773.1 0 5081 228 561 773.1 0 561 229 206 773.1 0 206 230 82 773.1 0 82 231 67 773.1 0 67 232 89 773.1 0 89 233 106 773.1 0 106 234 73 773.1 0 73 235 125 773.1 0 125 236 138 773.1 0 138 237 609 773.1 0 609 238 635 773.1 0 635 239 846 773.1 0 846 240 393 773.1 0 393 241 232 773.1 0 232 242 226 773.1 0 226 243 227 773.1 0 227 244 155 773.1 0 155 245 264 773.1 0 264 246 354 773.1 0 354 247 302 773.1 0 302 248 205 773.1 0 205 249 124 773.1 0 124 250 122 773.1 0 122 251 69 773.1 0 69 252 52 773.1 0 52 253 56 773.1 0 56 254 70 773.1 0 70 255 92 773.1 0 92 256 84 773.1 0 84 257 82 773.1 0 82 258 66 773.1 0 66 259 84 773.1 0 84 260 591 773.1 0 591 261 388 773.1 0 388 262 656 773.1 0 656 263 206 773.1 0 206 264 221 773.1 0 221 265 108 773.1 0 108 266 117 773.1 0 117 267 194 773.1 0 194 268 85 773.1 0 85 269 69 773.1 0 69 270 55 773.1 0 55 271 71 773.1 0 71 272 179 773.1 0 179 273 164 773.1 0 164 274 163 773.1 0 163 275 98 773.1 0 98 276 75 773.1 0 75 277 73 773.1 0 73 278 121 773.1 0 121 279 159 773.1 0 159 280 218 773.1 0 218 281 164 773.1 0 164 282 151 773.1 0 151 283 84 773.1 0 84 284 80 773.1 0 80 285 274 773.1 0 274 286 557 773.1 0 557 287 710 773.1 0 710 288 711 773.1 0 711 289 98 773.1 0 98 290 116 773.1 0 116 291 151 773.1 0 151 292 125 773.1 0 125 293 140 773.1 0 140 294 139 773.1 0 139 295 133 773.1 0 133 296 152 773.1 0 152 297 146 773.1 0 146 298 121 773.1 0 121 299 287 773.1 0 287 300 257 773.1 0 257 301 511 773.1 0 511 Finished in 9.39 s (70 us/read; 0.85 M reads/minute). === Summary === Total reads processed: 133,210 Reads with adapters: 60,084 (45.1%) Reads that were too short: 105 (0.1%) Reads written (passing filters): 59,979 (45.0%) Total basepairs processed: 37,317,129 bp Total written (filtered): 16,471,824 bp (44.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 60084 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 15 0.0 3 0 0 8 7 16 313 0.0 3 0 254 36 23 17 59007 0.0 3 55446 2926 500 135 18 142 0.0 3 6 115 7 14 19 104 0.0 3 2 1 66 35 20 124 0.0 3 7 5 1 111 21 3 0.0 3 0 0 0 3 22 147 0.0 3 0 0 0 147 23 2 0.0 3 0 0 0 2 27 7 0.0 3 0 0 0 7 28 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 30 2 0.0 3 0 0 0 2 31 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 42 4 0.0 3 0 0 0 4 44 2 0.0 3 0 0 0 2 48 4 0.0 3 1 0 0 3 49 1 0.0 3 0 0 0 1 50 2 0.0 3 0 0 1 1 51 2 0.0 3 0 0 0 2 53 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 5 0.0 3 0 0 0 5 68 1 0.0 3 0 0 0 1 71 4 0.0 3 0 0 0 4 72 1 0.0 3 0 0 0 1 75 2 0.0 3 0 0 0 2 76 3 0.0 3 0 0 0 3 77 1 0.0 3 0 0 0 1 78 2 0.0 3 0 0 0 2 79 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 85 2 0.0 3 0 0 0 2 87 1 0.0 3 0 0 0 1 88 3 0.0 3 0 0 1 2 89 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 91 2 0.0 3 0 0 0 2 93 2 0.0 3 0 0 1 1 94 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 98 2 0.0 3 1 0 0 1 101 3 0.0 3 0 0 0 3 102 1 0.0 3 0 0 0 1 103 2 0.0 3 0 0 0 2 107 4 0.0 3 0 0 0 4 109 2 0.0 3 0 0 0 2 110 2 0.0 3 0 0 0 2 112 1 0.0 3 0 0 0 1 114 3 0.0 3 1 0 0 2 116 1 0.0 3 0 0 0 1 121 3 0.0 3 0 0 0 3 123 3 0.0 3 0 0 0 3 124 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 129 6 0.0 3 0 0 0 6 130 15 0.0 3 0 0 0 15 132 1 0.0 3 0 0 0 1 133 5 0.0 3 0 0 0 5 134 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 2 0.0 3 0 0 0 2 150 5 0.0 3 0 0 0 5 154 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 157 2 0.0 3 0 0 0 2 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 4 0.0 3 0 0 0 4 165 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 168 2 0.0 3 0 0 0 2 169 3 0.0 3 0 0 0 3 172 1 0.0 3 0 0 0 1 180 2 0.0 3 0 0 0 2 181 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 192 4 0.0 3 0 0 0 4 194 9 0.0 3 0 0 1 8 195 2 0.0 3 0 0 0 2 199 1 0.0 3 0 0 0 1 203 2 0.0 3 0 0 0 2 208 2 0.0 3 0 0 0 2 209 3 0.0 3 0 0 0 3 210 4 0.0 3 0 0 0 4 211 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 215 2 0.0 3 0 0 0 2 218 2 0.0 3 0 0 0 2 219 2 0.0 3 0 0 0 2 221 2 0.0 3 0 0 0 2 222 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 270 2 0.0 3 0 0 0 2 279 4 0.0 3 0 0 0 4 281 4 0.0 3 0 0 0 4 282 5 0.0 3 0 0 0 5 284 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.29 s (55 us/read; 1.09 M reads/minute). === Summary === Total reads processed: 59,979 Reads with adapters: 1,349 (2.2%) Reads written (passing filters): 59,979 (100.0%) Total basepairs processed: 16,471,824 bp Total written (filtered): 16,467,716 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1349 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 43.5% C: 8.9% G: 45.5% T: 2.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1300 937.2 0 1300 4 45 234.3 0 45 5 3 58.6 0 3 13 1 0.0 1 0 1