This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CAGAGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 140.80 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 144,334 (4.6%) Reads that were too short: 13,915 (0.4%) Reads written (passing filters): 130,419 (4.1%) Total basepairs processed: 953,096,634 bp Total written (filtered): 37,126,234 bp (3.9%) === Adapter 1 === Sequence: CAGAGC; Type: regular 5'; Length: 6; Trimmed: 144334 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1511 773.1 0 1511 7 62725 773.1 0 62725 8 579 773.1 0 579 9 54715 773.1 0 54715 10 40 773.1 0 40 11 25 773.1 0 25 12 7 773.1 0 7 13 3 773.1 0 3 14 6 773.1 0 6 15 6 773.1 0 6 16 7 773.1 0 7 17 4 773.1 0 4 18 4 773.1 0 4 19 5 773.1 0 5 20 4 773.1 0 4 21 1 773.1 0 1 22 3 773.1 0 3 23 5 773.1 0 5 24 5 773.1 0 5 25 2 773.1 0 2 26 9 773.1 0 9 27 53 773.1 0 53 28 344 773.1 0 344 29 46 773.1 0 46 30 2 773.1 0 2 31 9 773.1 0 9 32 5 773.1 0 5 33 11 773.1 0 11 34 13 773.1 0 13 35 3 773.1 0 3 36 11 773.1 0 11 37 20 773.1 0 20 38 13 773.1 0 13 39 13 773.1 0 13 40 7 773.1 0 7 41 13 773.1 0 13 42 13 773.1 0 13 43 16 773.1 0 16 44 12 773.1 0 12 45 19 773.1 0 19 46 14 773.1 0 14 47 24 773.1 0 24 48 147 773.1 0 147 49 7 773.1 0 7 50 40 773.1 0 40 51 246 773.1 0 246 52 44 773.1 0 44 53 15 773.1 0 15 54 15 773.1 0 15 55 15 773.1 0 15 56 14 773.1 0 14 57 5 773.1 0 5 58 8 773.1 0 8 59 29 773.1 0 29 60 21 773.1 0 21 61 53 773.1 0 53 62 274 773.1 0 274 63 231 773.1 0 231 64 271 773.1 0 271 65 42 773.1 0 42 66 25 773.1 0 25 67 22 773.1 0 22 68 21 773.1 0 21 69 25 773.1 0 25 70 11 773.1 0 11 71 10 773.1 0 10 72 9 773.1 0 9 73 26 773.1 0 26 74 29 773.1 0 29 75 30 773.1 0 30 76 32 773.1 0 32 77 46 773.1 0 46 78 620 773.1 0 620 79 691 773.1 0 691 80 637 773.1 0 637 81 349 773.1 0 349 82 88 773.1 0 88 83 804 773.1 0 804 84 23 773.1 0 23 85 12 773.1 0 12 86 159 773.1 0 159 87 23 773.1 0 23 88 8 773.1 0 8 89 12 773.1 0 12 90 19 773.1 0 19 91 25 773.1 0 25 92 19 773.1 0 19 93 32 773.1 0 32 94 32 773.1 0 32 95 17 773.1 0 17 96 17 773.1 0 17 97 15 773.1 0 15 98 12 773.1 0 12 99 20 773.1 0 20 100 31 773.1 0 31 101 23 773.1 0 23 102 148 773.1 0 148 103 18 773.1 0 18 104 10 773.1 0 10 105 9 773.1 0 9 106 12 773.1 0 12 107 16 773.1 0 16 108 26 773.1 0 26 109 57 773.1 0 57 110 50 773.1 0 50 111 46 773.1 0 46 112 8 773.1 0 8 113 14 773.1 0 14 114 14 773.1 0 14 115 17 773.1 0 17 116 87 773.1 0 87 117 52 773.1 0 52 118 29 773.1 0 29 119 19 773.1 0 19 120 20 773.1 0 20 121 23 773.1 0 23 122 18 773.1 0 18 123 27 773.1 0 27 124 30 773.1 0 30 125 50 773.1 0 50 126 78 773.1 0 78 127 30 773.1 0 30 128 29 773.1 0 29 129 20 773.1 0 20 130 51 773.1 0 51 131 22 773.1 0 22 132 22 773.1 0 22 133 16 773.1 0 16 134 22 773.1 0 22 135 13 773.1 0 13 136 14 773.1 0 14 137 18 773.1 0 18 138 15 773.1 0 15 139 8 773.1 0 8 140 22 773.1 0 22 141 46 773.1 0 46 142 66 773.1 0 66 143 31 773.1 0 31 144 27 773.1 0 27 145 38 773.1 0 38 146 38 773.1 0 38 147 12 773.1 0 12 148 41 773.1 0 41 149 16 773.1 0 16 150 39 773.1 0 39 151 19 773.1 0 19 152 11 773.1 0 11 153 21 773.1 0 21 154 16 773.1 0 16 155 19 773.1 0 19 156 30 773.1 0 30 157 79 773.1 0 79 158 27 773.1 0 27 159 24 773.1 0 24 160 14 773.1 0 14 161 23 773.1 0 23 162 13 773.1 0 13 163 15 773.1 0 15 164 18 773.1 0 18 165 29 773.1 0 29 166 28 773.1 0 28 167 34 773.1 0 34 168 20 773.1 0 20 169 20 773.1 0 20 170 24 773.1 0 24 171 20 773.1 0 20 172 13 773.1 0 13 173 14 773.1 0 14 174 13 773.1 0 13 175 18 773.1 0 18 176 13 773.1 0 13 177 24 773.1 0 24 178 31 773.1 0 31 179 36 773.1 0 36 180 80 773.1 0 80 181 456 773.1 0 456 182 408 773.1 0 408 183 491 773.1 0 491 184 89 773.1 0 89 185 29 773.1 0 29 186 124 773.1 0 124 187 88 773.1 0 88 188 80 773.1 0 80 189 25 773.1 0 25 190 116 773.1 0 116 191 99 773.1 0 99 192 122 773.1 0 122 193 44 773.1 0 44 194 25 773.1 0 25 195 24 773.1 0 24 196 24 773.1 0 24 197 25 773.1 0 25 198 31 773.1 0 31 199 37 773.1 0 37 200 13 773.1 0 13 201 11 773.1 0 11 202 22 773.1 0 22 203 32 773.1 0 32 204 47 773.1 0 47 205 35 773.1 0 35 206 38 773.1 0 38 207 10 773.1 0 10 208 43 773.1 0 43 209 39 773.1 0 39 210 64 773.1 0 64 211 61 773.1 0 61 212 42 773.1 0 42 213 44 773.1 0 44 214 49 773.1 0 49 215 131 773.1 0 131 216 37 773.1 0 37 217 305 773.1 0 305 218 340 773.1 0 340 219 325 773.1 0 325 220 48 773.1 0 48 221 51 773.1 0 51 222 48 773.1 0 48 223 64 773.1 0 64 224 78 773.1 0 78 225 1153 773.1 0 1153 226 915 773.1 0 915 227 1838 773.1 0 1838 228 273 773.1 0 273 229 87 773.1 0 87 230 41 773.1 0 41 231 33 773.1 0 33 232 51 773.1 0 51 233 48 773.1 0 48 234 48 773.1 0 48 235 65 773.1 0 65 236 73 773.1 0 73 237 270 773.1 0 270 238 306 773.1 0 306 239 369 773.1 0 369 240 178 773.1 0 178 241 127 773.1 0 127 242 94 773.1 0 94 243 100 773.1 0 100 244 85 773.1 0 85 245 99 773.1 0 99 246 148 773.1 0 148 247 84 773.1 0 84 248 71 773.1 0 71 249 39 773.1 0 39 250 42 773.1 0 42 251 26 773.1 0 26 252 33 773.1 0 33 253 35 773.1 0 35 254 28 773.1 0 28 255 37 773.1 0 37 256 35 773.1 0 35 257 32 773.1 0 32 258 42 773.1 0 42 259 47 773.1 0 47 260 520 773.1 0 520 261 315 773.1 0 315 262 566 773.1 0 566 263 125 773.1 0 125 264 144 773.1 0 144 265 48 773.1 0 48 266 77 773.1 0 77 267 63 773.1 0 63 268 52 773.1 0 52 269 47 773.1 0 47 270 35 773.1 0 35 271 46 773.1 0 46 272 116 773.1 0 116 273 85 773.1 0 85 274 110 773.1 0 110 275 46 773.1 0 46 276 34 773.1 0 34 277 30 773.1 0 30 278 58 773.1 0 58 279 59 773.1 0 59 280 100 773.1 0 100 281 104 773.1 0 104 282 80 773.1 0 80 283 38 773.1 0 38 284 42 773.1 0 42 285 126 773.1 0 126 286 289 773.1 0 289 287 300 773.1 0 300 288 369 773.1 0 369 289 41 773.1 0 41 290 49 773.1 0 49 291 63 773.1 0 63 292 69 773.1 0 69 293 60 773.1 0 60 294 77 773.1 0 77 295 75 773.1 0 75 296 72 773.1 0 72 297 53 773.1 0 53 298 63 773.1 0 63 299 91 773.1 0 91 300 73 773.1 0 73 301 200 773.1 0 200 Finished in 9.68 s (74 us/read; 0.81 M reads/minute). === Summary === Total reads processed: 130,419 Reads with adapters: 64,610 (49.5%) Reads that were too short: 34 (0.0%) Reads written (passing filters): 64,576 (49.5%) Total basepairs processed: 37,126,234 bp Total written (filtered): 17,574,360 bp (47.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 64610 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 3 0.0 3 0 0 3 19 191 0.0 3 0 184 4 3 20 63057 0.0 4 62026 1001 27 3 21 140 0.0 4 21 119 22 9 0.0 4 0 1 7 0 1 23 74 0.0 4 33 0 0 38 3 24 2 0.0 4 1 0 0 0 1 31 1 0.0 4 1 61 1 0.0 4 0 0 0 0 1 85 1 0.0 4 1 86 1 0.0 4 0 0 0 1 94 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 6 0.0 4 0 0 0 0 6 127 1075 0.0 4 0 0 0 0 1075 128 7 0.0 4 1 0 0 0 6 130 4 0.0 4 2 0 0 0 2 135 2 0.0 4 0 0 0 0 2 150 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 1 171 1 0.0 4 1 176 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 198 1 0.0 4 1 222 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 10 0.0 4 0 0 0 0 10 229 5 0.0 4 0 0 0 0 5 230 1 0.0 4 0 0 0 0 1 239 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 270 1 0.0 4 0 0 0 0 1 277 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 289 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.35 s (52 us/read; 1.16 M reads/minute). === Summary === Total reads processed: 64,576 Reads with adapters: 947 (1.5%) Reads written (passing filters): 64,576 (100.0%) Total basepairs processed: 17,574,360 bp Total written (filtered): 17,559,618 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 947 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 11.8% C: 4.9% G: 80.7% T: 2.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 106 1009.0 0 106 4 64 252.2 0 64 5 2 63.1 0 2 6 1 15.8 0 1 8 1 1.0 0 1 9 7 0.2 0 4 3 10 25 0.1 1 2 23 11 640 0.0 1 3 637 12 65 0.0 1 0 65 13 4 0.0 1 0 4 15 2 0.0 1 0 2 21 1 0.0 1 1 152 1 0.0 1 0 1 162 3 0.0 1 2 1 171 1 0.0 1 1 209 1 0.0 1 1 210 19 0.0 1 17 2 225 4 0.0 1 4 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.