This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCCTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 139.47 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 112,364 (3.5%) Reads that were too short: 15,944 (0.5%) Reads written (passing filters): 96,420 (3.0%) Total basepairs processed: 953,096,634 bp Total written (filtered): 23,685,665 bp (2.5%) === Adapter 1 === Sequence: TTCCTT; Type: regular 5'; Length: 6; Trimmed: 112364 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 309 773.1 0 309 7 638 773.1 0 638 8 25281 773.1 0 25281 9 22727 773.1 0 22727 10 55 773.1 0 55 11 166 773.1 0 166 12 151 773.1 0 151 13 317 773.1 0 317 14 53 773.1 0 53 15 42 773.1 0 42 16 37 773.1 0 37 17 57 773.1 0 57 18 65 773.1 0 65 19 41 773.1 0 41 20 27 773.1 0 27 21 31 773.1 0 31 22 54 773.1 0 54 23 37 773.1 0 37 24 41 773.1 0 41 25 78 773.1 0 78 26 67 773.1 0 67 27 52 773.1 0 52 28 38 773.1 0 38 29 35 773.1 0 35 30 48 773.1 0 48 31 407 773.1 0 407 32 499 773.1 0 499 33 435 773.1 0 435 34 39 773.1 0 39 35 39 773.1 0 39 36 24 773.1 0 24 37 62 773.1 0 62 38 64 773.1 0 64 39 92 773.1 0 92 40 74 773.1 0 74 41 46 773.1 0 46 42 66 773.1 0 66 43 77 773.1 0 77 44 35 773.1 0 35 45 37 773.1 0 37 46 52 773.1 0 52 47 62 773.1 0 62 48 99 773.1 0 99 49 76 773.1 0 76 50 87 773.1 0 87 51 95 773.1 0 95 52 200 773.1 0 200 53 69 773.1 0 69 54 81 773.1 0 81 55 84 773.1 0 84 56 117 773.1 0 117 57 113 773.1 0 113 58 61 773.1 0 61 59 51 773.1 0 51 60 54 773.1 0 54 61 53 773.1 0 53 62 82 773.1 0 82 63 62 773.1 0 62 64 32 773.1 0 32 65 48 773.1 0 48 66 82 773.1 0 82 67 91 773.1 0 91 68 67 773.1 0 67 69 57 773.1 0 57 70 63 773.1 0 63 71 75 773.1 0 75 72 71 773.1 0 71 73 66 773.1 0 66 74 59 773.1 0 59 75 57 773.1 0 57 76 66 773.1 0 66 77 84 773.1 0 84 78 50 773.1 0 50 79 34 773.1 0 34 80 41 773.1 0 41 81 55 773.1 0 55 82 90 773.1 0 90 83 66 773.1 0 66 84 79 773.1 0 79 85 164 773.1 0 164 86 1079 773.1 0 1079 87 554 773.1 0 554 88 827 773.1 0 827 89 166 773.1 0 166 90 253 773.1 0 253 91 95 773.1 0 95 92 91 773.1 0 91 93 138 773.1 0 138 94 118 773.1 0 118 95 69 773.1 0 69 96 98 773.1 0 98 97 117 773.1 0 117 98 119 773.1 0 119 99 188 773.1 0 188 100 215 773.1 0 215 101 393 773.1 0 393 102 760 773.1 0 760 103 11726 773.1 0 11726 104 5726 773.1 0 5726 105 7785 773.1 0 7785 106 723 773.1 0 723 107 760 773.1 0 760 108 561 773.1 0 561 109 72 773.1 0 72 110 109 773.1 0 109 111 97 773.1 0 97 112 104 773.1 0 104 113 58 773.1 0 58 114 36 773.1 0 36 115 46 773.1 0 46 116 37 773.1 0 37 117 30 773.1 0 30 118 55 773.1 0 55 119 60 773.1 0 60 120 73 773.1 0 73 121 35 773.1 0 35 122 30 773.1 0 30 123 41 773.1 0 41 124 62 773.1 0 62 125 73 773.1 0 73 126 76 773.1 0 76 127 106 773.1 0 106 128 55 773.1 0 55 129 81 773.1 0 81 130 63 773.1 0 63 131 60 773.1 0 60 132 96 773.1 0 96 133 140 773.1 0 140 134 68 773.1 0 68 135 74 773.1 0 74 136 55 773.1 0 55 137 57 773.1 0 57 138 120 773.1 0 120 139 151 773.1 0 151 140 148 773.1 0 148 141 86 773.1 0 86 142 52 773.1 0 52 143 148 773.1 0 148 144 181 773.1 0 181 145 231 773.1 0 231 146 78 773.1 0 78 147 78 773.1 0 78 148 101 773.1 0 101 149 293 773.1 0 293 150 269 773.1 0 269 151 283 773.1 0 283 152 247 773.1 0 247 153 99 773.1 0 99 154 74 773.1 0 74 155 192 773.1 0 192 156 80 773.1 0 80 157 117 773.1 0 117 158 106 773.1 0 106 159 97 773.1 0 97 160 93 773.1 0 93 161 53 773.1 0 53 162 38 773.1 0 38 163 35 773.1 0 35 164 62 773.1 0 62 165 47 773.1 0 47 166 53 773.1 0 53 167 59 773.1 0 59 168 36 773.1 0 36 169 49 773.1 0 49 170 60 773.1 0 60 171 59 773.1 0 59 172 60 773.1 0 60 173 74 773.1 0 74 174 41 773.1 0 41 175 38 773.1 0 38 176 54 773.1 0 54 177 54 773.1 0 54 178 279 773.1 0 279 179 261 773.1 0 261 180 228 773.1 0 228 181 73 773.1 0 73 182 52 773.1 0 52 183 75 773.1 0 75 184 81 773.1 0 81 185 144 773.1 0 144 186 72 773.1 0 72 187 88 773.1 0 88 188 66 773.1 0 66 189 103 773.1 0 103 190 81 773.1 0 81 191 100 773.1 0 100 192 42 773.1 0 42 193 47 773.1 0 47 194 44 773.1 0 44 195 67 773.1 0 67 196 55 773.1 0 55 197 66 773.1 0 66 198 47 773.1 0 47 199 74 773.1 0 74 200 78 773.1 0 78 201 53 773.1 0 53 202 69 773.1 0 69 203 58 773.1 0 58 204 71 773.1 0 71 205 171 773.1 0 171 206 233 773.1 0 233 207 210 773.1 0 210 208 139 773.1 0 139 209 68 773.1 0 68 210 92 773.1 0 92 211 276 773.1 0 276 212 121 773.1 0 121 213 162 773.1 0 162 214 152 773.1 0 152 215 148 773.1 0 148 216 107 773.1 0 107 217 73 773.1 0 73 218 70 773.1 0 70 219 143 773.1 0 143 220 190 773.1 0 190 221 178 773.1 0 178 222 144 773.1 0 144 223 98 773.1 0 98 224 118 773.1 0 118 225 181 773.1 0 181 226 114 773.1 0 114 227 78 773.1 0 78 228 76 773.1 0 76 229 97 773.1 0 97 230 67 773.1 0 67 231 105 773.1 0 105 232 69 773.1 0 69 233 169 773.1 0 169 234 172 773.1 0 172 235 174 773.1 0 174 236 106 773.1 0 106 237 69 773.1 0 69 238 71 773.1 0 71 239 98 773.1 0 98 240 91 773.1 0 91 241 135 773.1 0 135 242 154 773.1 0 154 243 114 773.1 0 114 244 82 773.1 0 82 245 70 773.1 0 70 246 63 773.1 0 63 247 64 773.1 0 64 248 84 773.1 0 84 249 54 773.1 0 54 250 58 773.1 0 58 251 92 773.1 0 92 252 67 773.1 0 67 253 86 773.1 0 86 254 88 773.1 0 88 255 77 773.1 0 77 256 108 773.1 0 108 257 141 773.1 0 141 258 134 773.1 0 134 259 318 773.1 0 318 260 387 773.1 0 387 261 1885 773.1 0 1885 262 1391 773.1 0 1391 263 1226 773.1 0 1226 264 111 773.1 0 111 265 60 773.1 0 60 266 88 773.1 0 88 267 92 773.1 0 92 268 70 773.1 0 70 269 51 773.1 0 51 270 65 773.1 0 65 271 55 773.1 0 55 272 79 773.1 0 79 273 90 773.1 0 90 274 171 773.1 0 171 275 133 773.1 0 133 276 130 773.1 0 130 277 65 773.1 0 65 278 124 773.1 0 124 279 221 773.1 0 221 280 320 773.1 0 320 281 297 773.1 0 297 282 144 773.1 0 144 283 115 773.1 0 115 284 165 773.1 0 165 285 78 773.1 0 78 286 62 773.1 0 62 287 78 773.1 0 78 288 141 773.1 0 141 289 79 773.1 0 79 290 52 773.1 0 52 291 42 773.1 0 42 292 65 773.1 0 65 293 93 773.1 0 93 294 103 773.1 0 103 295 104 773.1 0 104 296 74 773.1 0 74 297 76 773.1 0 76 298 73 773.1 0 73 299 87 773.1 0 87 300 181 773.1 0 181 301 204 773.1 0 204 Finished in 6.14 s (64 us/read; 0.94 M reads/minute). === Summary === Total reads processed: 96,420 Reads with adapters: 25,296 (26.2%) Reads that were too short: 50 (0.1%) Reads written (passing filters): 25,246 (26.2%) Total basepairs processed: 23,685,665 bp Total written (filtered): 6,959,651 bp (29.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 25296 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 5 0.0 2 0 0 0 5 15 15 0.0 3 0 0 6 9 16 265 0.0 3 0 222 30 13 17 24703 0.0 3 23274 1194 191 44 18 65 0.0 3 4 54 3 4 19 70 0.0 3 0 1 37 32 20 69 0.0 3 0 0 0 69 21 1 0.0 3 0 1 33 5 0.0 3 0 0 0 5 34 5 0.0 3 0 0 0 5 37 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 2 0.0 3 0 0 0 2 67 2 0.0 3 0 0 0 2 69 2 0.0 3 0 0 0 2 72 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 75 5 0.0 3 0 0 0 5 84 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 91 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 96 4 0.0 3 0 0 0 4 98 2 0.0 3 0 0 0 2 99 4 0.0 3 0 0 0 4 100 2 0.0 3 0 0 0 2 106 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 142 3 0.0 3 0 0 0 3 145 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 180 2 0.0 3 0 0 0 2 182 3 0.0 3 0 0 1 2 185 6 0.0 3 0 0 0 6 187 2 0.0 3 0 0 0 2 188 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 195 2 0.0 3 0 0 0 2 201 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.33 s (53 us/read; 1.14 M reads/minute). === Summary === Total reads processed: 25,246 Reads with adapters: 244 (1.0%) Reads written (passing filters): 25,246 (100.0%) Total basepairs processed: 6,959,651 bp Total written (filtered): 6,958,901 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 244 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 68.4% C: 11.5% G: 15.6% T: 4.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 231 394.5 0 231 4 8 98.6 0 8 5 5 24.7 0 5