This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 142.45 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 366,136 (11.6%) Reads that were too short: 164,385 (5.2%) Reads written (passing filters): 201,751 (6.4%) Total basepairs processed: 953,096,634 bp Total written (filtered): 52,280,727 bp (5.5%) === Adapter 1 === Sequence: GTGTAG; Type: regular 5'; Length: 6; Trimmed: 366136 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1226 773.1 0 1226 7 64999 773.1 0 64999 8 52346 773.1 0 52346 9 125 773.1 0 125 10 47 773.1 0 47 11 53 773.1 0 53 12 27 773.1 0 27 13 44 773.1 0 44 14 42 773.1 0 42 15 29 773.1 0 29 16 57 773.1 0 57 17 45 773.1 0 45 18 65 773.1 0 65 19 75 773.1 0 75 20 9802 773.1 0 9802 21 9243 773.1 0 9243 22 164 773.1 0 164 23 127 773.1 0 127 24 50 773.1 0 50 25 43 773.1 0 43 26 515 773.1 0 515 27 35 773.1 0 35 28 43 773.1 0 43 29 37 773.1 0 37 30 44 773.1 0 44 31 48 773.1 0 48 32 44 773.1 0 44 33 7223 773.1 0 7223 34 685 773.1 0 685 35 37 773.1 0 37 36 47 773.1 0 47 37 49 773.1 0 49 38 70 773.1 0 70 39 91 773.1 0 91 40 52 773.1 0 52 41 50 773.1 0 50 42 49 773.1 0 49 43 62 773.1 0 62 44 64 773.1 0 64 45 72 773.1 0 72 46 50 773.1 0 50 47 55 773.1 0 55 48 65 773.1 0 65 49 57 773.1 0 57 50 96 773.1 0 96 51 47 773.1 0 47 52 67 773.1 0 67 53 112 773.1 0 112 54 51 773.1 0 51 55 73 773.1 0 73 56 66 773.1 0 66 57 81 773.1 0 81 58 97 773.1 0 97 59 78 773.1 0 78 60 88 773.1 0 88 61 96 773.1 0 96 62 96 773.1 0 96 63 124 773.1 0 124 64 83 773.1 0 83 65 105 773.1 0 105 66 139 773.1 0 139 67 116 773.1 0 116 68 132 773.1 0 132 69 165 773.1 0 165 70 146 773.1 0 146 71 178 773.1 0 178 72 109 773.1 0 109 73 164 773.1 0 164 74 186 773.1 0 186 75 435 773.1 0 435 76 1621 773.1 0 1621 77 1885 773.1 0 1885 78 3282 773.1 0 3282 79 213 773.1 0 213 80 353 773.1 0 353 81 424 773.1 0 424 82 540 773.1 0 540 83 451 773.1 0 451 84 342 773.1 0 342 85 370 773.1 0 370 86 133 773.1 0 133 87 157 773.1 0 157 88 557 773.1 0 557 89 164 773.1 0 164 90 143 773.1 0 143 91 191 773.1 0 191 92 191 773.1 0 191 93 168 773.1 0 168 94 154 773.1 0 154 95 176 773.1 0 176 96 153 773.1 0 153 97 168 773.1 0 168 98 160 773.1 0 160 99 186 773.1 0 186 100 172 773.1 0 172 101 187 773.1 0 187 102 153 773.1 0 153 103 192 773.1 0 192 104 216 773.1 0 216 105 173 773.1 0 173 106 211 773.1 0 211 107 202 773.1 0 202 108 216 773.1 0 216 109 206 773.1 0 206 110 196 773.1 0 196 111 264 773.1 0 264 112 272 773.1 0 272 113 226 773.1 0 226 114 210 773.1 0 210 115 260 773.1 0 260 116 251 773.1 0 251 117 255 773.1 0 255 118 231 773.1 0 231 119 228 773.1 0 228 120 248 773.1 0 248 121 245 773.1 0 245 122 203 773.1 0 203 123 434 773.1 0 434 124 277 773.1 0 277 125 274 773.1 0 274 126 265 773.1 0 265 127 256 773.1 0 256 128 277 773.1 0 277 129 359 773.1 0 359 130 2198 773.1 0 2198 131 1623 773.1 0 1623 132 2043 773.1 0 2043 133 250 773.1 0 250 134 293 773.1 0 293 135 277 773.1 0 277 136 1404 773.1 0 1404 137 1743 773.1 0 1743 138 2574 773.1 0 2574 139 752 773.1 0 752 140 560 773.1 0 560 141 348 773.1 0 348 142 303 773.1 0 303 143 613 773.1 0 613 144 227 773.1 0 227 145 214 773.1 0 214 146 515 773.1 0 515 147 408 773.1 0 408 148 641 773.1 0 641 149 285 773.1 0 285 150 247 773.1 0 247 151 245 773.1 0 245 152 263 773.1 0 263 153 233 773.1 0 233 154 251 773.1 0 251 155 201 773.1 0 201 156 250 773.1 0 250 157 243 773.1 0 243 158 213 773.1 0 213 159 187 773.1 0 187 160 265 773.1 0 265 161 276 773.1 0 276 162 373 773.1 0 373 163 235 773.1 0 235 164 256 773.1 0 256 165 313 773.1 0 313 166 244 773.1 0 244 167 254 773.1 0 254 168 354 773.1 0 354 169 184 773.1 0 184 170 225 773.1 0 225 171 278 773.1 0 278 172 251 773.1 0 251 173 217 773.1 0 217 174 200 773.1 0 200 175 217 773.1 0 217 176 257 773.1 0 257 177 225 773.1 0 225 178 210 773.1 0 210 179 247 773.1 0 247 180 193 773.1 0 193 181 367 773.1 0 367 182 217 773.1 0 217 183 225 773.1 0 225 184 241 773.1 0 241 185 239 773.1 0 239 186 359 773.1 0 359 187 327 773.1 0 327 188 286 773.1 0 286 189 363 773.1 0 363 190 309 773.1 0 309 191 238 773.1 0 238 192 319 773.1 0 319 193 275 773.1 0 275 194 291 773.1 0 291 195 302 773.1 0 302 196 370 773.1 0 370 197 482 773.1 0 482 198 323 773.1 0 323 199 251 773.1 0 251 200 217 773.1 0 217 201 248 773.1 0 248 202 275 773.1 0 275 203 215 773.1 0 215 204 225 773.1 0 225 205 238 773.1 0 238 206 257 773.1 0 257 207 226 773.1 0 226 208 279 773.1 0 279 209 290 773.1 0 290 210 295 773.1 0 295 211 323 773.1 0 323 212 260 773.1 0 260 213 335 773.1 0 335 214 626 773.1 0 626 215 313 773.1 0 313 216 263 773.1 0 263 217 269 773.1 0 269 218 281 773.1 0 281 219 288 773.1 0 288 220 312 773.1 0 312 221 792 773.1 0 792 222 379 773.1 0 379 223 318 773.1 0 318 224 404 773.1 0 404 225 492 773.1 0 492 226 714 773.1 0 714 227 820 773.1 0 820 228 891 773.1 0 891 229 640 773.1 0 640 230 801 773.1 0 801 231 787 773.1 0 787 232 640 773.1 0 640 233 799 773.1 0 799 234 606 773.1 0 606 235 395 773.1 0 395 236 438 773.1 0 438 237 613 773.1 0 613 238 1237 773.1 0 1237 239 561 773.1 0 561 240 709 773.1 0 709 241 1250 773.1 0 1250 242 727 773.1 0 727 243 675 773.1 0 675 244 931 773.1 0 931 245 653 773.1 0 653 246 1074 773.1 0 1074 247 825 773.1 0 825 248 1361 773.1 0 1361 249 1314 773.1 0 1314 250 2078 773.1 0 2078 251 40622 773.1 0 40622 252 28422 773.1 0 28422 253 47209 773.1 0 47209 254 723 773.1 0 723 255 317 773.1 0 317 256 487 773.1 0 487 257 485 773.1 0 485 258 687 773.1 0 687 259 357 773.1 0 357 260 378 773.1 0 378 261 309 773.1 0 309 262 335 773.1 0 335 263 278 773.1 0 278 264 307 773.1 0 307 265 379 773.1 0 379 266 399 773.1 0 399 267 312 773.1 0 312 268 297 773.1 0 297 269 341 773.1 0 341 270 381 773.1 0 381 271 361 773.1 0 361 272 368 773.1 0 368 273 284 773.1 0 284 274 251 773.1 0 251 275 255 773.1 0 255 276 318 773.1 0 318 277 304 773.1 0 304 278 501 773.1 0 501 279 273 773.1 0 273 280 278 773.1 0 278 281 374 773.1 0 374 282 234 773.1 0 234 283 398 773.1 0 398 284 410 773.1 0 410 285 529 773.1 0 529 286 401 773.1 0 401 287 372 773.1 0 372 288 332 773.1 0 332 289 289 773.1 0 289 290 395 773.1 0 395 291 487 773.1 0 487 292 403 773.1 0 403 293 313 773.1 0 313 294 344 773.1 0 344 295 324 773.1 0 324 296 337 773.1 0 337 297 615 773.1 0 615 298 795 773.1 0 795 299 509 773.1 0 509 300 422 773.1 0 422 301 390 773.1 0 390 Finished in 13.11 s (65 us/read; 0.92 M reads/minute). === Summary === Total reads processed: 201,751 Reads with adapters: 64,176 (31.8%) Reads that were too short: 185 (0.1%) Reads written (passing filters): 63,991 (31.7%) Total basepairs processed: 52,280,727 bp Total written (filtered): 17,673,327 bp (33.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 64176 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 17 0.0 2 0 0 0 17 15 71 0.0 3 0 0 24 47 16 389 0.0 3 0 273 58 58 17 62566 0.0 3 57231 4022 987 326 18 146 0.0 3 13 106 10 17 19 241 0.0 3 4 0 121 116 20 219 0.0 3 2 2 0 215 21 1 0.0 3 0 0 1 24 2 0.0 3 0 0 0 2 27 1 0.0 3 0 0 0 1 29 5 0.0 3 0 0 0 5 33 2 0.0 3 0 0 0 2 34 2 0.0 3 0 0 0 2 35 40 0.0 3 0 0 1 39 36 2 0.0 3 0 0 0 2 38 3 0.0 3 0 0 0 3 39 1 0.0 3 0 0 0 1 40 4 0.0 3 0 0 1 3 43 3 0.0 3 0 0 0 3 45 1 0.0 3 0 0 0 1 46 3 0.0 3 0 0 0 3 47 1 0.0 3 0 1 49 3 0.0 3 0 0 0 3 50 8 0.0 3 0 0 0 8 51 3 0.0 3 0 0 0 3 53 4 0.0 3 0 0 0 4 56 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 61 76 0.0 3 0 0 0 76 62 1 0.0 3 0 0 0 1 63 4 0.0 3 0 1 0 3 64 12 0.0 3 0 0 0 12 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 69 2 0.0 3 0 0 0 2 70 4 0.0 3 0 0 0 4 71 2 0.0 3 0 0 0 2 72 4 0.0 3 0 0 0 4 73 1 0.0 3 0 0 0 1 74 3 0.0 3 0 0 0 3 75 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 79 2 0.0 3 0 0 0 2 80 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 2 0.0 3 0 0 0 2 85 1 0.0 3 0 0 0 1 87 2 0.0 3 0 0 0 2 89 1 0.0 3 0 0 0 1 90 4 0.0 3 0 0 0 4 91 3 0.0 3 0 0 0 3 92 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 95 2 0.0 3 0 0 0 2 96 1 0.0 3 0 0 0 1 97 9 0.0 3 0 0 1 8 98 2 0.0 3 0 0 0 2 100 32 0.0 3 0 0 0 32 101 2 0.0 3 0 0 0 2 103 3 0.0 3 0 0 0 3 104 2 0.0 3 0 0 0 2 107 1 0.0 3 0 0 0 1 109 2 0.0 3 0 0 0 2 110 6 0.0 3 0 0 0 6 111 9 0.0 3 0 0 1 8 112 4 0.0 3 0 0 1 3 113 6 0.0 3 0 0 1 5 114 2 0.0 3 0 0 0 2 115 6 0.0 3 0 0 0 6 116 1 0.0 3 0 0 0 1 117 2 0.0 3 0 0 0 2 118 2 0.0 3 0 0 0 2 121 1 0.0 3 0 0 0 1 122 2 0.0 3 0 0 0 2 126 2 0.0 3 0 0 0 2 127 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 1 129 4 0.0 3 0 0 0 4 131 2 0.0 3 0 0 0 2 133 2 0.0 3 0 0 0 2 134 1 0.0 3 0 0 0 1 135 2 0.0 3 0 0 1 1 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 143 2 0.0 3 0 0 0 2 145 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 3 0.0 3 0 0 0 3 150 12 0.0 3 0 0 0 12 151 2 0.0 3 0 0 1 1 152 3 0.0 3 0 0 1 2 153 4 0.0 3 0 0 1 3 154 2 0.0 3 0 0 0 2 155 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 159 1 0.0 3 0 0 0 1 161 2 0.0 3 0 0 1 1 162 5 0.0 3 0 0 0 5 163 1 0.0 3 0 0 0 1 164 6 0.0 3 0 0 0 6 165 1 0.0 3 0 0 0 1 166 2 0.0 3 0 0 0 2 167 2 0.0 3 0 0 1 1 168 1 0.0 3 0 0 0 1 170 3 0.0 3 0 0 0 3 171 6 0.0 3 0 0 0 6 173 2 0.0 3 0 0 0 2 174 2 0.0 3 0 0 0 2 176 6 0.0 3 0 0 0 6 177 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 184 2 0.0 3 0 0 0 2 185 1 0.0 3 0 0 0 1 186 2 0.0 3 0 0 0 2 188 1 0.0 3 0 0 0 1 189 2 0.0 3 0 0 0 2 190 2 0.0 3 0 0 0 2 192 2 0.0 3 0 0 0 2 193 1 0.0 3 0 0 0 1 194 4 0.0 3 0 0 0 4 197 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 205 4 0.0 3 0 0 0 4 207 2 0.0 3 0 0 0 2 208 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 7 0.0 3 0 0 0 7 218 2 0.0 3 0 0 0 2 219 2 0.0 3 0 0 0 2 222 1 0.0 3 0 0 0 1 223 3 0.0 3 0 0 0 3 224 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 242 2 0.0 3 0 0 0 2 243 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 247 2 0.0 3 0 0 0 2 251 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 255 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 260 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 264 1 0.0 3 0 0 0 1 265 1 0.0 3 0 0 0 1 266 2 0.0 3 0 0 0 2 267 2 0.0 3 0 0 1 1 268 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 270 2 0.0 3 0 0 0 2 271 2 0.0 3 0 0 0 2 272 1 0.0 3 0 0 0 1 273 2 0.0 3 0 0 0 2 275 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 278 2 0.0 3 0 0 0 2 279 2 0.0 3 0 0 0 2 280 4 0.0 3 0 0 0 4 281 6 0.0 3 0 0 0 6 282 5 0.0 3 0 0 0 5 283 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.39 s (53 us/read; 1.13 M reads/minute). === Summary === Total reads processed: 63,991 Reads with adapters: 201 (0.3%) Reads written (passing filters): 63,991 (100.0%) Total basepairs processed: 17,673,327 bp Total written (filtered): 17,672,682 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 201 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 18.9% C: 41.3% G: 29.9% T: 10.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 176 999.9 0 176 4 12 250.0 0 12 5 9 62.5 0 9 6 4 15.6 0 4