This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 142.03 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 315,891 (10.0%) Reads that were too short: 159,472 (5.0%) Reads written (passing filters): 156,419 (4.9%) Total basepairs processed: 953,096,634 bp Total written (filtered): 42,731,057 bp (4.5%) === Adapter 1 === Sequence: GTGTAG; Type: regular 5'; Length: 6; Trimmed: 315891 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1174 773.1 0 1174 7 56704 773.1 0 56704 8 68097 773.1 0 68097 9 139 773.1 0 139 10 63 773.1 0 63 11 63 773.1 0 63 12 45 773.1 0 45 13 44 773.1 0 44 14 44 773.1 0 44 15 33 773.1 0 33 16 40 773.1 0 40 17 49 773.1 0 49 18 66 773.1 0 66 19 50 773.1 0 50 20 49 773.1 0 49 21 71 773.1 0 71 22 70 773.1 0 70 23 82 773.1 0 82 24 50 773.1 0 50 25 44 773.1 0 44 26 42 773.1 0 42 27 37 773.1 0 37 28 64 773.1 0 64 29 44 773.1 0 44 30 55 773.1 0 55 31 58 773.1 0 58 32 49 773.1 0 49 33 43 773.1 0 43 34 45 773.1 0 45 35 41 773.1 0 41 36 65 773.1 0 65 37 41 773.1 0 41 38 88 773.1 0 88 39 78 773.1 0 78 40 77 773.1 0 77 41 44 773.1 0 44 42 35 773.1 0 35 43 67 773.1 0 67 44 60 773.1 0 60 45 63 773.1 0 63 46 59 773.1 0 59 47 69 773.1 0 69 48 110 773.1 0 110 49 80 773.1 0 80 50 199 773.1 0 199 51 43 773.1 0 43 52 56 773.1 0 56 53 57 773.1 0 57 54 67 773.1 0 67 55 84 773.1 0 84 56 51 773.1 0 51 57 61 773.1 0 61 58 101 773.1 0 101 59 62 773.1 0 62 60 81 773.1 0 81 61 78 773.1 0 78 62 45 773.1 0 45 63 94 773.1 0 94 64 59 773.1 0 59 65 73 773.1 0 73 66 65 773.1 0 65 67 83 773.1 0 83 68 63 773.1 0 63 69 116 773.1 0 116 70 87 773.1 0 87 71 84 773.1 0 84 72 69 773.1 0 69 73 76 773.1 0 76 74 74 773.1 0 74 75 315 773.1 0 315 76 1380 773.1 0 1380 77 1427 773.1 0 1427 78 2170 773.1 0 2170 79 140 773.1 0 140 80 191 773.1 0 191 81 198 773.1 0 198 82 182 773.1 0 182 83 387 773.1 0 387 84 314 773.1 0 314 85 327 773.1 0 327 86 42 773.1 0 42 87 59 773.1 0 59 88 58 773.1 0 58 89 60 773.1 0 60 90 56 773.1 0 56 91 93 773.1 0 93 92 75 773.1 0 75 93 70 773.1 0 70 94 70 773.1 0 70 95 73 773.1 0 73 96 63 773.1 0 63 97 58 773.1 0 58 98 60 773.1 0 60 99 63 773.1 0 63 100 74 773.1 0 74 101 61 773.1 0 61 102 83 773.1 0 83 103 73 773.1 0 73 104 84 773.1 0 84 105 87 773.1 0 87 106 83 773.1 0 83 107 112 773.1 0 112 108 80 773.1 0 80 109 74 773.1 0 74 110 67 773.1 0 67 111 107 773.1 0 107 112 143 773.1 0 143 113 75 773.1 0 75 114 64 773.1 0 64 115 87 773.1 0 87 116 76 773.1 0 76 117 87 773.1 0 87 118 69 773.1 0 69 119 81 773.1 0 81 120 106 773.1 0 106 121 110 773.1 0 110 122 89 773.1 0 89 123 127 773.1 0 127 124 92 773.1 0 92 125 71 773.1 0 71 126 96 773.1 0 96 127 85 773.1 0 85 128 80 773.1 0 80 129 109 773.1 0 109 130 1525 773.1 0 1525 131 1069 773.1 0 1069 132 1355 773.1 0 1355 133 124 773.1 0 124 134 119 773.1 0 119 135 120 773.1 0 120 136 1230 773.1 0 1230 137 1615 773.1 0 1615 138 2154 773.1 0 2154 139 259 773.1 0 259 140 71 773.1 0 71 141 88 773.1 0 88 142 109 773.1 0 109 143 83 773.1 0 83 144 60 773.1 0 60 145 70 773.1 0 70 146 487 773.1 0 487 147 278 773.1 0 278 148 524 773.1 0 524 149 126 773.1 0 126 150 44 773.1 0 44 151 52 773.1 0 52 152 40 773.1 0 40 153 66 773.1 0 66 154 117 773.1 0 117 155 48 773.1 0 48 156 48 773.1 0 48 157 41 773.1 0 41 158 64 773.1 0 64 159 49 773.1 0 49 160 80 773.1 0 80 161 154 773.1 0 154 162 164 773.1 0 164 163 86 773.1 0 86 164 149 773.1 0 149 165 178 773.1 0 178 166 87 773.1 0 87 167 95 773.1 0 95 168 199 773.1 0 199 169 58 773.1 0 58 170 64 773.1 0 64 171 149 773.1 0 149 172 58 773.1 0 58 173 59 773.1 0 59 174 44 773.1 0 44 175 67 773.1 0 67 176 63 773.1 0 63 177 61 773.1 0 61 178 54 773.1 0 54 179 33 773.1 0 33 180 36 773.1 0 36 181 44 773.1 0 44 182 72 773.1 0 72 183 74 773.1 0 74 184 76 773.1 0 76 185 76 773.1 0 76 186 155 773.1 0 155 187 94 773.1 0 94 188 105 773.1 0 105 189 190 773.1 0 190 190 130 773.1 0 130 191 55 773.1 0 55 192 47 773.1 0 47 193 45 773.1 0 45 194 44 773.1 0 44 195 47 773.1 0 47 196 61 773.1 0 61 197 43 773.1 0 43 198 55 773.1 0 55 199 36 773.1 0 36 200 36 773.1 0 36 201 79 773.1 0 79 202 58 773.1 0 58 203 37 773.1 0 37 204 32 773.1 0 32 205 37 773.1 0 37 206 48 773.1 0 48 207 63 773.1 0 63 208 49 773.1 0 49 209 69 773.1 0 69 210 59 773.1 0 59 211 51 773.1 0 51 212 41 773.1 0 41 213 66 773.1 0 66 214 55 773.1 0 55 215 52 773.1 0 52 216 71 773.1 0 71 217 71 773.1 0 71 218 58 773.1 0 58 219 73 773.1 0 73 220 61 773.1 0 61 221 82 773.1 0 82 222 93 773.1 0 93 223 82 773.1 0 82 224 147 773.1 0 147 225 296 773.1 0 296 226 453 773.1 0 453 227 489 773.1 0 489 228 506 773.1 0 506 229 382 773.1 0 382 230 539 773.1 0 539 231 572 773.1 0 572 232 347 773.1 0 347 233 554 773.1 0 554 234 183 773.1 0 183 235 202 773.1 0 202 236 305 773.1 0 305 237 504 773.1 0 504 238 1175 773.1 0 1175 239 300 773.1 0 300 240 482 773.1 0 482 241 824 773.1 0 824 242 329 773.1 0 329 243 286 773.1 0 286 244 461 773.1 0 461 245 394 773.1 0 394 246 852 773.1 0 852 247 677 773.1 0 677 248 1375 773.1 0 1375 249 1159 773.1 0 1159 250 1838 773.1 0 1838 251 46970 773.1 0 46970 252 33644 773.1 0 33644 253 54894 773.1 0 54894 254 431 773.1 0 431 255 94 773.1 0 94 256 116 773.1 0 116 257 112 773.1 0 112 258 159 773.1 0 159 259 66 773.1 0 66 260 96 773.1 0 96 261 79 773.1 0 79 262 138 773.1 0 138 263 105 773.1 0 105 264 105 773.1 0 105 265 111 773.1 0 111 266 118 773.1 0 118 267 115 773.1 0 115 268 93 773.1 0 93 269 116 773.1 0 116 270 141 773.1 0 141 271 129 773.1 0 129 272 100 773.1 0 100 273 100 773.1 0 100 274 83 773.1 0 83 275 82 773.1 0 82 276 83 773.1 0 83 277 87 773.1 0 87 278 76 773.1 0 76 279 82 773.1 0 82 280 87 773.1 0 87 281 78 773.1 0 78 282 69 773.1 0 69 283 232 773.1 0 232 284 233 773.1 0 233 285 315 773.1 0 315 286 242 773.1 0 242 287 134 773.1 0 134 288 130 773.1 0 130 289 118 773.1 0 118 290 149 773.1 0 149 291 205 773.1 0 205 292 180 773.1 0 180 293 149 773.1 0 149 294 144 773.1 0 144 295 148 773.1 0 148 296 126 773.1 0 126 297 337 773.1 0 337 298 361 773.1 0 361 299 224 773.1 0 224 300 189 773.1 0 189 301 158 773.1 0 158 Finished in 11.55 s (74 us/read; 0.81 M reads/minute). === Summary === Total reads processed: 156,419 Reads with adapters: 70,950 (45.4%) Reads that were too short: 59 (0.0%) Reads written (passing filters): 70,891 (45.3%) Total basepairs processed: 42,731,057 bp Total written (filtered): 19,328,117 bp (45.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 70950 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 11 0.0 3 0 0 11 19 232 0.0 3 0 224 5 2 1 20 70133 0.0 4 68905 1177 48 3 21 193 0.0 4 37 155 1 22 11 0.0 4 1 0 9 0 1 23 44 0.0 4 0 0 0 40 4 24 3 0.0 4 0 0 0 1 2 26 1 0.0 4 0 0 0 0 1 29 1 0.0 4 1 33 1 0.0 4 1 54 12 0.0 4 0 0 0 0 12 63 1 0.0 4 0 0 0 0 1 66 3 0.0 4 0 0 0 0 3 85 1 0.0 4 1 97 1 0.0 4 0 0 0 0 1 99 4 0.0 4 0 0 0 0 4 100 4 0.0 4 0 0 0 0 4 113 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 121 6 0.0 4 0 0 0 0 6 126 3 0.0 4 0 0 0 0 3 127 236 0.0 4 0 0 0 0 236 128 2 0.0 4 0 0 0 0 2 150 1 0.0 4 0 1 152 1 0.0 4 1 153 2 0.0 4 2 154 6 0.0 4 2 2 1 0 1 155 3 0.0 4 2 1 156 4 0.0 4 1 1 1 1 157 2 0.0 4 1 0 0 0 1 159 3 0.0 4 0 0 0 0 3 160 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 0 0 1 228 12 0.0 4 0 0 0 0 12 229 1 0.0 4 0 0 0 0 1 237 2 0.0 4 0 0 0 0 2 248 1 0.0 4 0 0 0 0 1 263 2 0.0 4 0 0 0 0 2 265 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.84 s (54 us/read; 1.11 M reads/minute). === Summary === Total reads processed: 70,891 Reads with adapters: 3,757 (5.3%) Reads written (passing filters): 70,891 (100.0%) Total basepairs processed: 19,328,117 bp Total written (filtered): 19,286,241 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3757 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.9% C: 3.0% G: 93.1% T: 2.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 49 1107.7 0 49 4 10 276.9 0 10 5 3 69.2 0 3 7 1 4.3 0 1 9 282 0.3 0 6 276 10 676 0.1 1 22 654 11 2709 0.0 1 13 2696 12 12 0.0 1 1 11 13 1 0.0 1 0 1 15 1 0.0 1 0 1 123 2 0.0 1 2 151 2 0.0 1 0 2 190 4 0.0 1 0 4 209 2 0.0 1 1 1 224 3 0.0 1 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.