This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACGCGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 139.93 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 285,837 (9.0%) Reads that were too short: 243,130 (7.7%) Reads written (passing filters): 42,707 (1.3%) Total basepairs processed: 953,096,634 bp Total written (filtered): 9,404,803 bp (1.0%) === Adapter 1 === Sequence: ACGCGC; Type: regular 5'; Length: 6; Trimmed: 285837 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3553 773.1 0 3553 7 7753 773.1 0 7753 8 717 773.1 0 717 9 8969 773.1 0 8969 10 37 773.1 0 37 11 25 773.1 0 25 12 17 773.1 0 17 13 31 773.1 0 31 14 15 773.1 0 15 15 17 773.1 0 17 16 24 773.1 0 24 17 19 773.1 0 19 18 40 773.1 0 40 19 49 773.1 0 49 20 57 773.1 0 57 21 35 773.1 0 35 22 36 773.1 0 36 23 22 773.1 0 22 24 9 773.1 0 9 25 17 773.1 0 17 26 15 773.1 0 15 27 21 773.1 0 21 28 11 773.1 0 11 29 14 773.1 0 14 30 14 773.1 0 14 31 18 773.1 0 18 32 16 773.1 0 16 33 17 773.1 0 17 34 15 773.1 0 15 35 27 773.1 0 27 36 20 773.1 0 20 37 17 773.1 0 17 38 15 773.1 0 15 39 27 773.1 0 27 40 24 773.1 0 24 41 30 773.1 0 30 42 23 773.1 0 23 43 29 773.1 0 29 44 18 773.1 0 18 45 34 773.1 0 34 46 58 773.1 0 58 47 212 773.1 0 212 48 57 773.1 0 57 49 68 773.1 0 68 50 23 773.1 0 23 51 25 773.1 0 25 52 29 773.1 0 29 53 49 773.1 0 49 54 52 773.1 0 52 55 93 773.1 0 93 56 40 773.1 0 40 57 25 773.1 0 25 58 21 773.1 0 21 59 30 773.1 0 30 60 32 773.1 0 32 61 30 773.1 0 30 62 39 773.1 0 39 63 42 773.1 0 42 64 38 773.1 0 38 65 21 773.1 0 21 66 21 773.1 0 21 67 23 773.1 0 23 68 12 773.1 0 12 69 50 773.1 0 50 70 47 773.1 0 47 71 84 773.1 0 84 72 132 773.1 0 132 73 117 773.1 0 117 74 166 773.1 0 166 75 14 773.1 0 14 76 27 773.1 0 27 77 12 773.1 0 12 78 14 773.1 0 14 79 13 773.1 0 13 80 13 773.1 0 13 81 22 773.1 0 22 82 22 773.1 0 22 83 60 773.1 0 60 84 48 773.1 0 48 85 43 773.1 0 43 86 44 773.1 0 44 87 40 773.1 0 40 88 19 773.1 0 19 89 29 773.1 0 29 90 28 773.1 0 28 91 30 773.1 0 30 92 12 773.1 0 12 93 20 773.1 0 20 94 18 773.1 0 18 95 22 773.1 0 22 96 17 773.1 0 17 97 19 773.1 0 19 98 14 773.1 0 14 99 13 773.1 0 13 100 19 773.1 0 19 101 18 773.1 0 18 102 16 773.1 0 16 103 26 773.1 0 26 104 22 773.1 0 22 105 32 773.1 0 32 106 16 773.1 0 16 107 28 773.1 0 28 108 31 773.1 0 31 109 19 773.1 0 19 110 20 773.1 0 20 111 19 773.1 0 19 112 30 773.1 0 30 113 50 773.1 0 50 114 63 773.1 0 63 115 41 773.1 0 41 116 78 773.1 0 78 117 57 773.1 0 57 118 72 773.1 0 72 119 28 773.1 0 28 120 42 773.1 0 42 121 27 773.1 0 27 122 64 773.1 0 64 123 328 773.1 0 328 124 275 773.1 0 275 125 706 773.1 0 706 126 132 773.1 0 132 127 46 773.1 0 46 128 46 773.1 0 46 129 44 773.1 0 44 130 46 773.1 0 46 131 57 773.1 0 57 132 33 773.1 0 33 133 33 773.1 0 33 134 46 773.1 0 46 135 48 773.1 0 48 136 49 773.1 0 49 137 40 773.1 0 40 138 33 773.1 0 33 139 58 773.1 0 58 140 39 773.1 0 39 141 71 773.1 0 71 142 30 773.1 0 30 143 37 773.1 0 37 144 44 773.1 0 44 145 42 773.1 0 42 146 38 773.1 0 38 147 71 773.1 0 71 148 24 773.1 0 24 149 71 773.1 0 71 150 245 773.1 0 245 151 183 773.1 0 183 152 241 773.1 0 241 153 94 773.1 0 94 154 64 773.1 0 64 155 57 773.1 0 57 156 60 773.1 0 60 157 45 773.1 0 45 158 56 773.1 0 56 159 114 773.1 0 114 160 419 773.1 0 419 161 276 773.1 0 276 162 542 773.1 0 542 163 130 773.1 0 130 164 153 773.1 0 153 165 1258 773.1 0 1258 166 922 773.1 0 922 167 1550 773.1 0 1550 168 293 773.1 0 293 169 131 773.1 0 131 170 106 773.1 0 106 171 107 773.1 0 107 172 124 773.1 0 124 173 97 773.1 0 97 174 70 773.1 0 70 175 98 773.1 0 98 176 135 773.1 0 135 177 157 773.1 0 157 178 114 773.1 0 114 179 214 773.1 0 214 180 187 773.1 0 187 181 159 773.1 0 159 182 118 773.1 0 118 183 219 773.1 0 219 184 102 773.1 0 102 185 759 773.1 0 759 186 928 773.1 0 928 187 1472 773.1 0 1472 188 776 773.1 0 776 189 728 773.1 0 728 190 94 773.1 0 94 191 105 773.1 0 105 192 97 773.1 0 97 193 84 773.1 0 84 194 103 773.1 0 103 195 150 773.1 0 150 196 191 773.1 0 191 197 228 773.1 0 228 198 211 773.1 0 211 199 171 773.1 0 171 200 263 773.1 0 263 201 106 773.1 0 106 202 66 773.1 0 66 203 65 773.1 0 65 204 84 773.1 0 84 205 77 773.1 0 77 206 63 773.1 0 63 207 74 773.1 0 74 208 60 773.1 0 60 209 88 773.1 0 88 210 263 773.1 0 263 211 73 773.1 0 73 212 108 773.1 0 108 213 100 773.1 0 100 214 175 773.1 0 175 215 116 773.1 0 116 216 150 773.1 0 150 217 268 773.1 0 268 218 498 773.1 0 498 219 665 773.1 0 665 220 1113 773.1 0 1113 221 5828 773.1 0 5828 222 7569 773.1 0 7569 223 7207 773.1 0 7207 224 3679 773.1 0 3679 225 955 773.1 0 955 226 663 773.1 0 663 227 1061 773.1 0 1061 228 238 773.1 0 238 229 251 773.1 0 251 230 235 773.1 0 235 231 354 773.1 0 354 232 520 773.1 0 520 233 713 773.1 0 713 234 883 773.1 0 883 235 1912 773.1 0 1912 236 4438 773.1 0 4438 237 58741 773.1 0 58741 238 38194 773.1 0 38194 239 71166 773.1 0 71166 240 3814 773.1 0 3814 241 1019 773.1 0 1019 242 318 773.1 0 318 243 148 773.1 0 148 244 164 773.1 0 164 245 2513 773.1 0 2513 246 3501 773.1 0 3501 247 4340 773.1 0 4340 248 2316 773.1 0 2316 249 1107 773.1 0 1107 250 847 773.1 0 847 251 263 773.1 0 263 252 108 773.1 0 108 253 39 773.1 0 39 254 41 773.1 0 41 255 47 773.1 0 47 256 98 773.1 0 98 257 51 773.1 0 51 258 51 773.1 0 51 259 45 773.1 0 45 260 112 773.1 0 112 261 115 773.1 0 115 262 166 773.1 0 166 263 71 773.1 0 71 264 87 773.1 0 87 265 64 773.1 0 64 266 111 773.1 0 111 267 274 773.1 0 274 268 738 773.1 0 738 269 712 773.1 0 712 270 930 773.1 0 930 271 383 773.1 0 383 272 192 773.1 0 192 273 124 773.1 0 124 274 211 773.1 0 211 275 118 773.1 0 118 276 120 773.1 0 120 277 109 773.1 0 109 278 123 773.1 0 123 279 115 773.1 0 115 280 204 773.1 0 204 281 635 773.1 0 635 282 423 773.1 0 423 283 593 773.1 0 593 284 123 773.1 0 123 285 183 773.1 0 183 286 191 773.1 0 191 287 491 773.1 0 491 288 438 773.1 0 438 289 854 773.1 0 854 290 651 773.1 0 651 291 492 773.1 0 492 292 227 773.1 0 227 293 132 773.1 0 132 294 838 773.1 0 838 295 698 773.1 0 698 296 704 773.1 0 704 297 201 773.1 0 201 298 116 773.1 0 116 299 136 773.1 0 136 300 92 773.1 0 92 301 290 773.1 0 290 Finished in 2.61 s (61 us/read; 0.98 M reads/minute). === Summary === Total reads processed: 42,707 Reads with adapters: 12,783 (29.9%) Reads that were too short: 174 (0.4%) Reads written (passing filters): 12,609 (29.5%) Total basepairs processed: 9,404,803 bp Total written (filtered): 3,437,721 bp (36.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 12783 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 8 0.0 3 0 0 3 5 16 61 0.0 3 0 48 7 6 17 9314 0.0 3 8719 479 87 29 18 353 0.0 3 234 91 25 3 19 52 0.0 3 1 19 15 17 20 2601 0.0 3 2368 165 39 29 21 45 0.0 3 0 5 0 40 22 11 0.0 3 1 0 8 2 23 2 0.0 3 0 0 0 2 24 14 0.0 3 0 0 1 13 28 1 0.0 3 0 0 0 1 30 2 0.0 3 0 0 0 2 32 58 0.0 3 0 0 0 58 33 1 0.0 3 0 0 0 1 35 2 0.0 3 0 0 1 1 38 2 0.0 3 0 0 0 2 42 5 0.0 3 0 0 0 5 45 2 0.0 3 0 0 1 1 47 3 0.0 3 0 0 0 3 48 45 0.0 3 0 0 3 42 51 5 0.0 3 0 0 0 5 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 55 2 0.0 3 0 0 0 2 57 15 0.0 3 0 0 0 15 59 2 0.0 3 0 0 0 2 60 1 0.0 3 0 0 0 1 61 2 0.0 3 0 0 0 2 66 4 0.0 3 0 0 0 4 68 1 0.0 3 0 0 0 1 69 2 0.0 3 0 0 0 2 74 1 0.0 3 0 0 1 77 1 0.0 3 0 0 0 1 79 2 0.0 3 0 0 0 2 80 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 91 1 0.0 3 0 1 94 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 96 2 0.0 3 0 0 0 2 97 2 0.0 3 0 0 0 2 98 2 0.0 3 0 0 0 2 99 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 101 3 0.0 3 0 0 0 3 102 2 0.0 3 0 0 0 2 103 1 0.0 3 0 0 0 1 107 5 0.0 3 0 0 0 5 108 5 0.0 3 0 0 0 5 109 2 0.0 3 0 0 0 2 110 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 118 7 0.0 3 0 0 0 7 119 7 0.0 3 0 0 0 7 120 2 0.0 3 0 0 0 2 121 4 0.0 3 0 0 0 4 122 3 0.0 3 0 0 0 3 123 3 0.0 3 0 0 1 2 125 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 129 3 0.0 3 0 0 0 3 135 2 0.0 3 0 0 0 2 136 7 0.0 3 0 0 0 7 138 1 0.0 3 0 0 1 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 160 5 0.0 3 0 0 0 5 161 1 0.0 3 0 0 0 1 163 4 0.0 3 0 0 0 4 164 2 0.0 3 0 0 0 2 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 175 2 0.0 3 0 0 0 2 178 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 1 217 24 0.0 3 0 0 0 24 225 2 0.0 3 0 0 0 2 226 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 259 1 0.0 3 0 0 0 1 264 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.69 s (55 us/read; 1.10 M reads/minute). === Summary === Total reads processed: 12,609 Reads with adapters: 496 (3.9%) Reads written (passing filters): 12,609 (100.0%) Total basepairs processed: 3,437,721 bp Total written (filtered): 3,436,220 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 496 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 65.7% C: 11.7% G: 20.4% T: 2.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 484 197.0 0 484 4 11 49.3 0 11 5 1 12.3 0 1