This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCTAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 144.92 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 494,908 (15.6%) Reads that were too short: 9,610 (0.3%) Reads written (passing filters): 485,298 (15.3%) Total basepairs processed: 953,096,634 bp Total written (filtered): 101,594,440 bp (10.7%) === Adapter 1 === Sequence: CCTAAT; Type: regular 5'; Length: 6; Trimmed: 494908 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1205 773.1 0 1205 7 89159 773.1 0 89159 8 6963 773.1 0 6963 9 74615 773.1 0 74615 10 143 773.1 0 143 11 37 773.1 0 37 12 51 773.1 0 51 13 83 773.1 0 83 14 80 773.1 0 80 15 79 773.1 0 79 16 52 773.1 0 52 17 30 773.1 0 30 18 39 773.1 0 39 19 55 773.1 0 55 20 44 773.1 0 44 21 52 773.1 0 52 22 29 773.1 0 29 23 39 773.1 0 39 24 55 773.1 0 55 25 70 773.1 0 70 26 116 773.1 0 116 27 759 773.1 0 759 28 1414 773.1 0 1414 29 803 773.1 0 803 30 36 773.1 0 36 31 53 773.1 0 53 32 43 773.1 0 43 33 59 773.1 0 59 34 70 773.1 0 70 35 58 773.1 0 58 36 41 773.1 0 41 37 68 773.1 0 68 38 31 773.1 0 31 39 52 773.1 0 52 40 43 773.1 0 43 41 49 773.1 0 49 42 72 773.1 0 72 43 64 773.1 0 64 44 69 773.1 0 69 45 55 773.1 0 55 46 57 773.1 0 57 47 55 773.1 0 55 48 55 773.1 0 55 49 52 773.1 0 52 50 75 773.1 0 75 51 94 773.1 0 94 52 61 773.1 0 61 53 56 773.1 0 56 54 41 773.1 0 41 55 42 773.1 0 42 56 60 773.1 0 60 57 184 773.1 0 184 58 298 773.1 0 298 59 563 773.1 0 563 60 63 773.1 0 63 61 38 773.1 0 38 62 47 773.1 0 47 63 53 773.1 0 53 64 47 773.1 0 47 65 70 773.1 0 70 66 40 773.1 0 40 67 53 773.1 0 53 68 67 773.1 0 67 69 51 773.1 0 51 70 69 773.1 0 69 71 75 773.1 0 75 72 37 773.1 0 37 73 55 773.1 0 55 74 67 773.1 0 67 75 58 773.1 0 58 76 61 773.1 0 61 77 70 773.1 0 70 78 60 773.1 0 60 79 49 773.1 0 49 80 72 773.1 0 72 81 154 773.1 0 154 82 55 773.1 0 55 83 60 773.1 0 60 84 78 773.1 0 78 85 59 773.1 0 59 86 48 773.1 0 48 87 43 773.1 0 43 88 51 773.1 0 51 89 71 773.1 0 71 90 104 773.1 0 104 91 214 773.1 0 214 92 128 773.1 0 128 93 132 773.1 0 132 94 133 773.1 0 133 95 171 773.1 0 171 96 295 773.1 0 295 97 103 773.1 0 103 98 103 773.1 0 103 99 89 773.1 0 89 100 85 773.1 0 85 101 148 773.1 0 148 102 203 773.1 0 203 103 314 773.1 0 314 104 328 773.1 0 328 105 512 773.1 0 512 106 1043 773.1 0 1043 107 2836 773.1 0 2836 108 40445 773.1 0 40445 109 34537 773.1 0 34537 110 27990 773.1 0 27990 111 314 773.1 0 314 112 73 773.1 0 73 113 61 773.1 0 61 114 48 773.1 0 48 115 62 773.1 0 62 116 114 773.1 0 114 117 125 773.1 0 125 118 80 773.1 0 80 119 98 773.1 0 98 120 120 773.1 0 120 121 194 773.1 0 194 122 105 773.1 0 105 123 107 773.1 0 107 124 99 773.1 0 99 125 119 773.1 0 119 126 90 773.1 0 90 127 250 773.1 0 250 128 250 773.1 0 250 129 361 773.1 0 361 130 158 773.1 0 158 131 227 773.1 0 227 132 202 773.1 0 202 133 274 773.1 0 274 134 3593 773.1 0 3593 135 4316 773.1 0 4316 136 7603 773.1 0 7603 137 219 773.1 0 219 138 99 773.1 0 99 139 79 773.1 0 79 140 138 773.1 0 138 141 205 773.1 0 205 142 270 773.1 0 270 143 195 773.1 0 195 144 238 773.1 0 238 145 242 773.1 0 242 146 308 773.1 0 308 147 552 773.1 0 552 148 176 773.1 0 176 149 291 773.1 0 291 150 420 773.1 0 420 151 233 773.1 0 233 152 255 773.1 0 255 153 457 773.1 0 457 154 803 773.1 0 803 155 2627 773.1 0 2627 156 1883 773.1 0 1883 157 2221 773.1 0 2221 158 1560 773.1 0 1560 159 1924 773.1 0 1924 160 38020 773.1 0 38020 161 71361 773.1 0 71361 162 48121 773.1 0 48121 163 257 773.1 0 257 164 120 773.1 0 120 165 94 773.1 0 94 166 87 773.1 0 87 167 68 773.1 0 68 168 40 773.1 0 40 169 107 773.1 0 107 170 110 773.1 0 110 171 54 773.1 0 54 172 45 773.1 0 45 173 54 773.1 0 54 174 87 773.1 0 87 175 99 773.1 0 99 176 61 773.1 0 61 177 58 773.1 0 58 178 52 773.1 0 52 179 98 773.1 0 98 180 101 773.1 0 101 181 98 773.1 0 98 182 81 773.1 0 81 183 85 773.1 0 85 184 56 773.1 0 56 185 58 773.1 0 58 186 66 773.1 0 66 187 63 773.1 0 63 188 53 773.1 0 53 189 43 773.1 0 43 190 70 773.1 0 70 191 153 773.1 0 153 192 102 773.1 0 102 193 147 773.1 0 147 194 61 773.1 0 61 195 54 773.1 0 54 196 55 773.1 0 55 197 61 773.1 0 61 198 51 773.1 0 51 199 55 773.1 0 55 200 42 773.1 0 42 201 50 773.1 0 50 202 57 773.1 0 57 203 85 773.1 0 85 204 91 773.1 0 91 205 66 773.1 0 66 206 66 773.1 0 66 207 59 773.1 0 59 208 68 773.1 0 68 209 67 773.1 0 67 210 70 773.1 0 70 211 51 773.1 0 51 212 58 773.1 0 58 213 39 773.1 0 39 214 49 773.1 0 49 215 48 773.1 0 48 216 45 773.1 0 45 217 52 773.1 0 52 218 66 773.1 0 66 219 83 773.1 0 83 220 87 773.1 0 87 221 107 773.1 0 107 222 259 773.1 0 259 223 303 773.1 0 303 224 144 773.1 0 144 225 170 773.1 0 170 226 61 773.1 0 61 227 54 773.1 0 54 228 49 773.1 0 49 229 49 773.1 0 49 230 54 773.1 0 54 231 62 773.1 0 62 232 99 773.1 0 99 233 67 773.1 0 67 234 116 773.1 0 116 235 113 773.1 0 113 236 158 773.1 0 158 237 148 773.1 0 148 238 70 773.1 0 70 239 54 773.1 0 54 240 62 773.1 0 62 241 77 773.1 0 77 242 85 773.1 0 85 243 72 773.1 0 72 244 49 773.1 0 49 245 45 773.1 0 45 246 45 773.1 0 45 247 38 773.1 0 38 248 58 773.1 0 58 249 62 773.1 0 62 250 134 773.1 0 134 251 245 773.1 0 245 252 254 773.1 0 254 253 395 773.1 0 395 254 634 773.1 0 634 255 300 773.1 0 300 256 252 773.1 0 252 257 71 773.1 0 71 258 74 773.1 0 74 259 42 773.1 0 42 260 56 773.1 0 56 261 112 773.1 0 112 262 107 773.1 0 107 263 110 773.1 0 110 264 65 773.1 0 65 265 81 773.1 0 81 266 53 773.1 0 53 267 46 773.1 0 46 268 48 773.1 0 48 269 64 773.1 0 64 270 88 773.1 0 88 271 100 773.1 0 100 272 102 773.1 0 102 273 51 773.1 0 51 274 54 773.1 0 54 275 57 773.1 0 57 276 51 773.1 0 51 277 74 773.1 0 74 278 121 773.1 0 121 279 113 773.1 0 113 280 119 773.1 0 119 281 106 773.1 0 106 282 177 773.1 0 177 283 181 773.1 0 181 284 119 773.1 0 119 285 58 773.1 0 58 286 44 773.1 0 44 287 42 773.1 0 42 288 29 773.1 0 29 289 49 773.1 0 49 290 76 773.1 0 76 291 82 773.1 0 82 292 83 773.1 0 83 293 72 773.1 0 72 294 51 773.1 0 51 295 62 773.1 0 62 296 68 773.1 0 68 297 64 773.1 0 64 298 41 773.1 0 41 299 76 773.1 0 76 300 61 773.1 0 61 301 59 773.1 0 59 Finished in 27.52 s (57 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 485,298 Reads with adapters: 92,625 (19.1%) Reads that were too short: 561 (0.1%) Reads written (passing filters): 92,064 (19.0%) Total basepairs processed: 101,594,440 bp Total written (filtered): 25,010,773 bp (24.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 92625 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 19 0.0 2 0 0 0 19 15 28 0.0 3 0 0 18 10 16 549 0.0 3 0 458 62 29 17 87993 0.0 3 83729 3552 590 122 18 177 0.0 3 10 145 10 12 19 147 0.0 3 0 0 81 66 20 208 0.0 3 0 0 0 208 22 1 0.0 3 0 0 0 1 26 2 0.0 3 0 0 0 2 27 2 0.0 3 0 0 0 2 30 2 0.0 3 0 0 0 2 31 2643 0.0 3 0 0 15 2628 33 2 0.0 3 0 0 0 2 34 182 0.0 3 0 0 2 180 37 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 54 2 0.0 3 0 0 0 2 55 4 0.0 3 0 0 0 4 57 35 0.0 3 0 0 0 35 60 5 0.0 3 0 0 0 5 63 24 0.0 3 0 0 0 24 64 1 0.0 3 0 0 0 1 65 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 82 25 0.0 3 0 0 0 25 84 2 0.0 3 0 0 0 2 88 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 90 2 0.0 3 0 0 0 2 91 10 0.0 3 0 0 0 10 92 1 0.0 3 0 0 0 1 93 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 95 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 104 18 0.0 3 0 0 0 18 106 2 0.0 3 0 0 0 2 108 5 0.0 3 0 0 0 5 109 4 0.0 3 0 0 0 4 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 5 0.0 3 0 0 0 5 115 3 0.0 3 0 0 0 3 116 2 0.0 3 0 0 0 2 117 6 0.0 3 0 0 0 6 118 2 0.0 3 0 0 0 2 120 1 0.0 3 0 0 0 1 121 2 0.0 3 0 0 0 2 122 2 0.0 3 0 0 0 2 123 15 0.0 3 0 0 0 15 124 2 0.0 3 0 0 0 2 127 1 0.0 3 0 0 0 1 129 2 0.0 3 0 0 0 2 131 1 0.0 3 0 0 0 1 132 112 0.0 3 0 0 3 109 133 11 0.0 3 0 0 1 10 134 6 0.0 3 0 0 1 5 135 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 140 2 0.0 3 0 0 0 2 145 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 158 9 0.0 3 0 0 0 9 159 23 0.0 3 0 0 0 23 160 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 171 6 0.0 3 0 0 0 6 173 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 177 2 0.0 3 0 0 0 2 179 4 0.0 3 0 0 0 4 180 4 0.0 3 0 0 0 4 181 2 0.0 3 0 0 0 2 184 2 0.0 3 0 0 0 2 185 1 0.0 3 0 0 0 1 188 2 0.0 3 0 0 0 2 189 1 0.0 3 0 0 0 1 191 2 0.0 3 0 0 0 2 192 3 0.0 3 0 0 0 3 194 5 0.0 3 0 0 0 5 195 2 0.0 3 0 0 0 2 205 10 0.0 3 0 0 1 9 214 1 0.0 3 0 0 0 1 217 189 0.0 3 0 0 13 176 218 2 0.0 3 0 0 0 2 221 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 226 1 0.0 3 1 229 2 0.0 3 0 0 0 2 230 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 238 3 0.0 3 0 0 0 3 240 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 278 2 0.0 3 0 0 0 2 279 6 0.0 3 0 0 0 6 280 7 0.0 3 0 0 0 7 281 7 0.0 3 0 0 0 7 282 4 0.0 3 0 0 0 4 283 5 0.0 3 0 0 0 5 284 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.95 s (54 us/read; 1.12 M reads/minute). === Summary === Total reads processed: 92,064 Reads with adapters: 444 (0.5%) Reads written (passing filters): 92,064 (100.0%) Total basepairs processed: 25,010,773 bp Total written (filtered): 25,009,389 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 444 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 15.5% C: 65.8% G: 9.7% T: 9.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 418 1438.5 0 418 4 8 359.6 0 8 5 13 89.9 0 13 6 4 22.5 0 4 9 1 0.4 0 0 1