This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCTAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 147.41 s (47 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 462,801 (14.6%) Reads that were too short: 6,463 (0.2%) Reads written (passing filters): 456,338 (14.4%) Total basepairs processed: 953,096,634 bp Total written (filtered): 98,740,283 bp (10.4%) === Adapter 1 === Sequence: CCTAAT; Type: regular 5'; Length: 6; Trimmed: 462801 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 928 773.1 0 928 7 86864 773.1 0 86864 8 5521 773.1 0 5521 9 84651 773.1 0 84651 10 142 773.1 0 142 11 30 773.1 0 30 12 36 773.1 0 36 13 64 773.1 0 64 14 60 773.1 0 60 15 88 773.1 0 88 16 29 773.1 0 29 17 25 773.1 0 25 18 32 773.1 0 32 19 39 773.1 0 39 20 38 773.1 0 38 21 59 773.1 0 59 22 40 773.1 0 40 23 39 773.1 0 39 24 36 773.1 0 36 25 37 773.1 0 37 26 54 773.1 0 54 27 176 773.1 0 176 28 228 773.1 0 228 29 139 773.1 0 139 30 36 773.1 0 36 31 28 773.1 0 28 32 39 773.1 0 39 33 40 773.1 0 40 34 45 773.1 0 45 35 41 773.1 0 41 36 37 773.1 0 37 37 54 773.1 0 54 38 29 773.1 0 29 39 37 773.1 0 37 40 38 773.1 0 38 41 50 773.1 0 50 42 41 773.1 0 41 43 51 773.1 0 51 44 35 773.1 0 35 45 52 773.1 0 52 46 38 773.1 0 38 47 46 773.1 0 46 48 29 773.1 0 29 49 43 773.1 0 43 50 81 773.1 0 81 51 78 773.1 0 78 52 46 773.1 0 46 53 51 773.1 0 51 54 28 773.1 0 28 55 39 773.1 0 39 56 57 773.1 0 57 57 147 773.1 0 147 58 217 773.1 0 217 59 258 773.1 0 258 60 49 773.1 0 49 61 34 773.1 0 34 62 29 773.1 0 29 63 60 773.1 0 60 64 53 773.1 0 53 65 61 773.1 0 61 66 36 773.1 0 36 67 45 773.1 0 45 68 51 773.1 0 51 69 34 773.1 0 34 70 46 773.1 0 46 71 70 773.1 0 70 72 44 773.1 0 44 73 46 773.1 0 46 74 58 773.1 0 58 75 35 773.1 0 35 76 35 773.1 0 35 77 44 773.1 0 44 78 59 773.1 0 59 79 47 773.1 0 47 80 39 773.1 0 39 81 157 773.1 0 157 82 44 773.1 0 44 83 56 773.1 0 56 84 60 773.1 0 60 85 49 773.1 0 49 86 57 773.1 0 57 87 47 773.1 0 47 88 59 773.1 0 59 89 71 773.1 0 71 90 122 773.1 0 122 91 214 773.1 0 214 92 120 773.1 0 120 93 128 773.1 0 128 94 107 773.1 0 107 95 147 773.1 0 147 96 294 773.1 0 294 97 102 773.1 0 102 98 69 773.1 0 69 99 75 773.1 0 75 100 70 773.1 0 70 101 148 773.1 0 148 102 173 773.1 0 173 103 273 773.1 0 273 104 330 773.1 0 330 105 493 773.1 0 493 106 1094 773.1 0 1094 107 2495 773.1 0 2495 108 46741 773.1 0 46741 109 39882 773.1 0 39882 110 32577 773.1 0 32577 111 362 773.1 0 362 112 47 773.1 0 47 113 35 773.1 0 35 114 24 773.1 0 24 115 57 773.1 0 57 116 101 773.1 0 101 117 70 773.1 0 70 118 80 773.1 0 80 119 77 773.1 0 77 120 97 773.1 0 97 121 178 773.1 0 178 122 73 773.1 0 73 123 67 773.1 0 67 124 78 773.1 0 78 125 76 773.1 0 76 126 66 773.1 0 66 127 169 773.1 0 169 128 164 773.1 0 164 129 205 773.1 0 205 130 106 773.1 0 106 131 159 773.1 0 159 132 146 773.1 0 146 133 186 773.1 0 186 134 3190 773.1 0 3190 135 3811 773.1 0 3811 136 7023 773.1 0 7023 137 112 773.1 0 112 138 74 773.1 0 74 139 50 773.1 0 50 140 90 773.1 0 90 141 69 773.1 0 69 142 108 773.1 0 108 143 110 773.1 0 110 144 159 773.1 0 159 145 175 773.1 0 175 146 233 773.1 0 233 147 417 773.1 0 417 148 147 773.1 0 147 149 205 773.1 0 205 150 347 773.1 0 347 151 185 773.1 0 185 152 196 773.1 0 196 153 386 773.1 0 386 154 634 773.1 0 634 155 2964 773.1 0 2964 156 1996 773.1 0 1996 157 2247 773.1 0 2247 158 973 773.1 0 973 159 1153 773.1 0 1153 160 27207 773.1 0 27207 161 53469 773.1 0 53469 162 35554 773.1 0 35554 163 152 773.1 0 152 164 70 773.1 0 70 165 59 773.1 0 59 166 83 773.1 0 83 167 45 773.1 0 45 168 36 773.1 0 36 169 82 773.1 0 82 170 117 773.1 0 117 171 38 773.1 0 38 172 30 773.1 0 30 173 30 773.1 0 30 174 46 773.1 0 46 175 58 773.1 0 58 176 29 773.1 0 29 177 45 773.1 0 45 178 37 773.1 0 37 179 45 773.1 0 45 180 81 773.1 0 81 181 69 773.1 0 69 182 32 773.1 0 32 183 44 773.1 0 44 184 42 773.1 0 42 185 40 773.1 0 40 186 31 773.1 0 31 187 42 773.1 0 42 188 32 773.1 0 32 189 30 773.1 0 30 190 42 773.1 0 42 191 103 773.1 0 103 192 76 773.1 0 76 193 94 773.1 0 94 194 43 773.1 0 43 195 30 773.1 0 30 196 27 773.1 0 27 197 35 773.1 0 35 198 29 773.1 0 29 199 33 773.1 0 33 200 37 773.1 0 37 201 42 773.1 0 42 202 46 773.1 0 46 203 51 773.1 0 51 204 69 773.1 0 69 205 46 773.1 0 46 206 38 773.1 0 38 207 30 773.1 0 30 208 72 773.1 0 72 209 41 773.1 0 41 210 37 773.1 0 37 211 25 773.1 0 25 212 29 773.1 0 29 213 32 773.1 0 32 214 28 773.1 0 28 215 41 773.1 0 41 216 43 773.1 0 43 217 52 773.1 0 52 218 64 773.1 0 64 219 41 773.1 0 41 220 51 773.1 0 51 221 71 773.1 0 71 222 189 773.1 0 189 223 241 773.1 0 241 224 95 773.1 0 95 225 100 773.1 0 100 226 37 773.1 0 37 227 45 773.1 0 45 228 40 773.1 0 40 229 30 773.1 0 30 230 42 773.1 0 42 231 47 773.1 0 47 232 60 773.1 0 60 233 55 773.1 0 55 234 89 773.1 0 89 235 92 773.1 0 92 236 125 773.1 0 125 237 145 773.1 0 145 238 41 773.1 0 41 239 35 773.1 0 35 240 27 773.1 0 27 241 37 773.1 0 37 242 52 773.1 0 52 243 37 773.1 0 37 244 33 773.1 0 33 245 37 773.1 0 37 246 14 773.1 0 14 247 38 773.1 0 38 248 31 773.1 0 31 249 45 773.1 0 45 250 111 773.1 0 111 251 259 773.1 0 259 252 234 773.1 0 234 253 293 773.1 0 293 254 291 773.1 0 291 255 94 773.1 0 94 256 95 773.1 0 95 257 27 773.1 0 27 258 61 773.1 0 61 259 33 773.1 0 33 260 46 773.1 0 46 261 78 773.1 0 78 262 85 773.1 0 85 263 79 773.1 0 79 264 38 773.1 0 38 265 39 773.1 0 39 266 32 773.1 0 32 267 20 773.1 0 20 268 33 773.1 0 33 269 38 773.1 0 38 270 61 773.1 0 61 271 47 773.1 0 47 272 68 773.1 0 68 273 41 773.1 0 41 274 38 773.1 0 38 275 46 773.1 0 46 276 39 773.1 0 39 277 62 773.1 0 62 278 92 773.1 0 92 279 98 773.1 0 98 280 71 773.1 0 71 281 62 773.1 0 62 282 88 773.1 0 88 283 112 773.1 0 112 284 85 773.1 0 85 285 51 773.1 0 51 286 43 773.1 0 43 287 26 773.1 0 26 288 32 773.1 0 32 289 31 773.1 0 31 290 47 773.1 0 47 291 58 773.1 0 58 292 62 773.1 0 62 293 38 773.1 0 38 294 33 773.1 0 33 295 40 773.1 0 40 296 45 773.1 0 45 297 37 773.1 0 37 298 27 773.1 0 27 299 43 773.1 0 43 300 39 773.1 0 39 301 49 773.1 0 49 Finished in 28.61 s (63 us/read; 0.96 M reads/minute). === Summary === Total reads processed: 456,338 Reads with adapters: 91,480 (20.0%) Reads that were too short: 29 (0.0%) Reads written (passing filters): 91,451 (20.0%) Total basepairs processed: 98,740,283 bp Total written (filtered): 24,859,623 bp (25.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 91480 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 9 0.0 3 0 0 8 1 19 218 0.0 3 0 205 7 4 2 20 90732 0.0 4 89335 1344 49 2 2 21 213 0.0 4 29 177 7 22 17 0.0 4 0 2 13 1 1 23 53 0.0 4 1 0 1 43 8 24 1 0.0 4 0 0 0 1 28 1 0.0 4 0 0 1 35 1 0.0 4 1 46 1 0.0 4 1 61 2 0.0 4 0 0 0 0 2 77 8 0.0 4 0 0 0 0 8 81 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 104 1 0.0 4 1 122 2 0.0 4 0 0 0 0 2 123 2 0.0 4 0 0 0 1 1 124 1 0.0 4 1 126 2 0.0 4 0 0 0 0 2 127 187 0.0 4 0 0 0 0 187 137 1 0.0 4 0 1 151 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 154 1 0.0 4 0 0 0 0 1 155 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 1 189 1 0.0 4 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 3 0.0 4 0 0 0 0 3 232 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 251 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 1 280 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 283 2 0.0 4 0 0 0 0 2 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.09 s (56 us/read; 1.08 M reads/minute). === Summary === Total reads processed: 91,451 Reads with adapters: 14,676 (16.0%) Reads written (passing filters): 91,451 (100.0%) Total basepairs processed: 24,859,623 bp Total written (filtered): 24,718,652 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 14676 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.7% C: 2.1% G: 96.0% T: 1.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 19 1428.9 0 19 4 21 357.2 0 21 5 9 89.3 0 9 6 1 22.3 0 1 7 1 5.6 0 1 8 2 1.4 0 2 9 9272 0.3 0 206 9066 10 4254 0.1 1 123 4131 11 1051 0.0 1 5 1046 12 12 0.0 1 0 12 13 16 0.0 1 5 11 22 1 0.0 1 1 23 1 0.0 1 1 120 2 0.0 1 2 121 1 0.0 1 1 156 4 0.0 1 4 161 1 0.0 1 1 208 8 0.0 1 4 4 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.