This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGGCCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 140.38 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 203,991 (6.4%) Reads that were too short: 72,662 (2.3%) Reads written (passing filters): 131,329 (4.1%) Total basepairs processed: 953,096,634 bp Total written (filtered): 33,741,101 bp (3.5%) === Adapter 1 === Sequence: CGGCCA; Type: regular 5'; Length: 6; Trimmed: 203991 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 9805 773.1 0 9805 7 38311 773.1 0 38311 8 2026 773.1 0 2026 9 45996 773.1 0 45996 10 80 773.1 0 80 11 184 773.1 0 184 12 67 773.1 0 67 13 17 773.1 0 17 14 24 773.1 0 24 15 12 773.1 0 12 16 22 773.1 0 22 17 23 773.1 0 23 18 15 773.1 0 15 19 30 773.1 0 30 20 33 773.1 0 33 21 47 773.1 0 47 22 31 773.1 0 31 23 21 773.1 0 21 24 36 773.1 0 36 25 22 773.1 0 22 26 21 773.1 0 21 27 28 773.1 0 28 28 18 773.1 0 18 29 15 773.1 0 15 30 25 773.1 0 25 31 29 773.1 0 29 32 58 773.1 0 58 33 28 773.1 0 28 34 28 773.1 0 28 35 27 773.1 0 27 36 20 773.1 0 20 37 41 773.1 0 41 38 37 773.1 0 37 39 33 773.1 0 33 40 61 773.1 0 61 41 298 773.1 0 298 42 343 773.1 0 343 43 247 773.1 0 247 44 30 773.1 0 30 45 25 773.1 0 25 46 34 773.1 0 34 47 39 773.1 0 39 48 37 773.1 0 37 49 44 773.1 0 44 50 65 773.1 0 65 51 66 773.1 0 66 52 57 773.1 0 57 53 30 773.1 0 30 54 45 773.1 0 45 55 48 773.1 0 48 56 42 773.1 0 42 57 24 773.1 0 24 58 31 773.1 0 31 59 30 773.1 0 30 60 23 773.1 0 23 61 18 773.1 0 18 62 33 773.1 0 33 63 46 773.1 0 46 64 266 773.1 0 266 65 245 773.1 0 245 66 266 773.1 0 266 67 25 773.1 0 25 68 29 773.1 0 29 69 30 773.1 0 30 70 35 773.1 0 35 71 41 773.1 0 41 72 57 773.1 0 57 73 110 773.1 0 110 74 765 773.1 0 765 75 488 773.1 0 488 76 566 773.1 0 566 77 27 773.1 0 27 78 42 773.1 0 42 79 32 773.1 0 32 80 46 773.1 0 46 81 40 773.1 0 40 82 29 773.1 0 29 83 34 773.1 0 34 84 30 773.1 0 30 85 23 773.1 0 23 86 33 773.1 0 33 87 142 773.1 0 142 88 132 773.1 0 132 89 236 773.1 0 236 90 26 773.1 0 26 91 23 773.1 0 23 92 46 773.1 0 46 93 41 773.1 0 41 94 31 773.1 0 31 95 30 773.1 0 30 96 27 773.1 0 27 97 25 773.1 0 25 98 29 773.1 0 29 99 40 773.1 0 40 100 39 773.1 0 39 101 23 773.1 0 23 102 25 773.1 0 25 103 21 773.1 0 21 104 29 773.1 0 29 105 32 773.1 0 32 106 18 773.1 0 18 107 17 773.1 0 17 108 27 773.1 0 27 109 30 773.1 0 30 110 25 773.1 0 25 111 28 773.1 0 28 112 32 773.1 0 32 113 30 773.1 0 30 114 29 773.1 0 29 115 38 773.1 0 38 116 31 773.1 0 31 117 44 773.1 0 44 118 47 773.1 0 47 119 28 773.1 0 28 120 30 773.1 0 30 121 23 773.1 0 23 122 35 773.1 0 35 123 34 773.1 0 34 124 35 773.1 0 35 125 62 773.1 0 62 126 50 773.1 0 50 127 64 773.1 0 64 128 57 773.1 0 57 129 43 773.1 0 43 130 25 773.1 0 25 131 38 773.1 0 38 132 58 773.1 0 58 133 35 773.1 0 35 134 45 773.1 0 45 135 37 773.1 0 37 136 35 773.1 0 35 137 76 773.1 0 76 138 70 773.1 0 70 139 54 773.1 0 54 140 27 773.1 0 27 141 35 773.1 0 35 142 88 773.1 0 88 143 92 773.1 0 92 144 73 773.1 0 73 145 44 773.1 0 44 146 59 773.1 0 59 147 58 773.1 0 58 148 65 773.1 0 65 149 100 773.1 0 100 150 194 773.1 0 194 151 1275 773.1 0 1275 152 1082 773.1 0 1082 153 905 773.1 0 905 154 349 773.1 0 349 155 243 773.1 0 243 156 2871 773.1 0 2871 157 2872 773.1 0 2872 158 3110 773.1 0 3110 159 727 773.1 0 727 160 109 773.1 0 109 161 85 773.1 0 85 162 73 773.1 0 73 163 114 773.1 0 114 164 603 773.1 0 603 165 344 773.1 0 344 166 351 773.1 0 351 167 182 773.1 0 182 168 196 773.1 0 196 169 178 773.1 0 178 170 525 773.1 0 525 171 900 773.1 0 900 172 691 773.1 0 691 173 257 773.1 0 257 174 127 773.1 0 127 175 208 773.1 0 208 176 1155 773.1 0 1155 177 1464 773.1 0 1464 178 1436 773.1 0 1436 179 670 773.1 0 670 180 273 773.1 0 273 181 220 773.1 0 220 182 242 773.1 0 242 183 146 773.1 0 146 184 102 773.1 0 102 185 112 773.1 0 112 186 100 773.1 0 100 187 59 773.1 0 59 188 225 773.1 0 225 189 192 773.1 0 192 190 143 773.1 0 143 191 55 773.1 0 55 192 48 773.1 0 48 193 41 773.1 0 41 194 65 773.1 0 65 195 75 773.1 0 75 196 82 773.1 0 82 197 48 773.1 0 48 198 57 773.1 0 57 199 60 773.1 0 60 200 236 773.1 0 236 201 299 773.1 0 299 202 192 773.1 0 192 203 74 773.1 0 74 204 47 773.1 0 47 205 73 773.1 0 73 206 71 773.1 0 71 207 68 773.1 0 68 208 44 773.1 0 44 209 43 773.1 0 43 210 49 773.1 0 49 211 33 773.1 0 33 212 42 773.1 0 42 213 42 773.1 0 42 214 87 773.1 0 87 215 153 773.1 0 153 216 131 773.1 0 131 217 54 773.1 0 54 218 60 773.1 0 60 219 75 773.1 0 75 220 57 773.1 0 57 221 59 773.1 0 59 222 67 773.1 0 67 223 81 773.1 0 81 224 88 773.1 0 88 225 91 773.1 0 91 226 79 773.1 0 79 227 82 773.1 0 82 228 115 773.1 0 115 229 144 773.1 0 144 230 176 773.1 0 176 231 95 773.1 0 95 232 277 773.1 0 277 233 820 773.1 0 820 234 420 773.1 0 420 235 364 773.1 0 364 236 554 773.1 0 554 237 314 773.1 0 314 238 482 773.1 0 482 239 126 773.1 0 126 240 165 773.1 0 165 241 76 773.1 0 76 242 77 773.1 0 77 243 87 773.1 0 87 244 74 773.1 0 74 245 106 773.1 0 106 246 69 773.1 0 69 247 86 773.1 0 86 248 167 773.1 0 167 249 501 773.1 0 501 250 496 773.1 0 496 251 456 773.1 0 456 252 211 773.1 0 211 253 837 773.1 0 837 254 559 773.1 0 559 255 910 773.1 0 910 256 159 773.1 0 159 257 342 773.1 0 342 258 455 773.1 0 455 259 800 773.1 0 800 260 1020 773.1 0 1020 261 6042 773.1 0 6042 262 9920 773.1 0 9920 263 13565 773.1 0 13565 264 10258 773.1 0 10258 265 4441 773.1 0 4441 266 92 773.1 0 92 267 74 773.1 0 74 268 81 773.1 0 81 269 95 773.1 0 95 270 81 773.1 0 81 271 52 773.1 0 52 272 66 773.1 0 66 273 91 773.1 0 91 274 99 773.1 0 99 275 85 773.1 0 85 276 113 773.1 0 113 277 282 773.1 0 282 278 215 773.1 0 215 279 128 773.1 0 128 280 136 773.1 0 136 281 129 773.1 0 129 282 254 773.1 0 254 283 209 773.1 0 209 284 185 773.1 0 185 285 123 773.1 0 123 286 119 773.1 0 119 287 872 773.1 0 872 288 1487 773.1 0 1487 289 927 773.1 0 927 290 179 773.1 0 179 291 229 773.1 0 229 292 621 773.1 0 621 293 589 773.1 0 589 294 481 773.1 0 481 295 561 773.1 0 561 296 472 773.1 0 472 297 691 773.1 0 691 298 1367 773.1 0 1367 299 1455 773.1 0 1455 300 1979 773.1 0 1979 301 335 773.1 0 335 Finished in 8.84 s (67 us/read; 0.89 M reads/minute). === Summary === Total reads processed: 131,329 Reads with adapters: 49,798 (37.9%) Reads that were too short: 360 (0.3%) Reads written (passing filters): 49,438 (37.6%) Total basepairs processed: 33,741,101 bp Total written (filtered): 13,498,891 bp (40.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 49798 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 6 0.0 2 0 0 0 6 15 507 0.0 3 0 0 20 487 16 266 0.0 3 0 217 30 19 17 48202 0.0 3 45465 2277 365 95 18 178 0.0 3 5 100 8 65 19 125 0.0 3 0 2 82 41 20 49 0.0 3 2 0 0 47 21 1 0.0 3 0 0 1 23 2 0.0 3 0 0 0 2 24 2 0.0 3 0 0 0 2 27 1 0.0 3 1 28 1 0.0 3 0 0 0 1 29 4 0.0 3 0 0 0 4 31 3 0.0 3 1 0 1 1 32 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 35 2 0.0 3 0 0 0 2 36 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 38 4 0.0 3 0 0 0 4 39 5 0.0 3 0 0 0 5 41 25 0.0 3 0 0 1 24 42 11 0.0 3 1 0 0 10 43 2 0.0 3 0 0 0 2 44 1 0.0 3 0 0 0 1 46 2 0.0 3 0 0 0 2 47 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 54 2 0.0 3 0 0 0 2 55 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 24 0.0 3 0 0 1 23 67 2 0.0 3 0 0 0 2 68 4 0.0 3 0 0 0 4 69 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 75 7 0.0 3 0 0 0 7 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 9 0.0 3 0 0 0 9 87 2 0.0 3 0 0 0 2 88 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 3 0.0 3 0 0 2 1 95 1 0.0 3 0 0 0 1 98 5 0.0 3 0 0 0 5 99 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 103 2 0.0 3 0 0 0 2 104 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 106 4 0.0 3 0 0 0 4 107 3 0.0 3 0 0 0 3 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 1 115 3 0.0 3 0 0 1 2 116 14 0.0 3 0 0 0 14 117 12 0.0 3 0 0 1 11 118 5 0.0 3 0 0 0 5 120 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 31 0.0 3 0 0 1 30 128 2 0.0 3 0 0 0 2 129 4 0.0 3 0 0 0 4 130 21 0.0 3 0 0 2 19 131 3 0.0 3 0 0 0 3 132 1 0.0 3 0 0 0 1 135 3 0.0 3 0 0 0 3 136 5 0.0 3 0 0 1 4 137 2 0.0 3 0 0 0 2 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 144 1 0.0 3 0 0 0 1 145 19 0.0 3 0 0 2 17 146 1 0.0 3 1 147 3 0.0 3 0 0 0 3 151 9 0.0 3 0 0 0 9 154 4 0.0 3 0 0 0 4 156 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 165 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 0 2 168 1 0.0 3 0 0 1 169 1 0.0 3 0 0 0 1 176 2 0.0 3 0 0 0 2 178 2 0.0 3 0 0 0 2 180 3 0.0 3 0 0 0 3 181 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 187 2 0.0 3 0 0 0 2 188 2 0.0 3 0 0 0 2 199 2 0.0 3 0 0 0 2 202 3 0.0 3 0 0 1 2 203 1 0.0 3 0 0 0 1 205 6 0.0 3 0 0 0 6 211 3 0.0 3 0 0 0 3 214 2 0.0 3 0 0 0 2 216 1 0.0 3 0 0 0 1 217 71 0.0 3 0 0 1 70 221 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 1 225 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 242 3 0.0 3 0 0 0 3 244 1 0.0 3 0 0 0 1 275 2 0.0 3 0 0 0 2 276 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 5 0.0 3 0 0 0 5 281 7 0.0 3 0 0 1 6 282 4 0.0 3 0 0 0 4 284 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.74 s (55 us/read; 1.08 M reads/minute). === Summary === Total reads processed: 49,438 Reads with adapters: 2,191 (4.4%) Reads written (passing filters): 49,438 (100.0%) Total basepairs processed: 13,498,891 bp Total written (filtered): 13,492,265 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2191 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 69.8% C: 14.2% G: 14.5% T: 1.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2150 772.5 0 2150 4 38 193.1 0 38 5 2 48.3 0 2 14 1 0.0 1 0 1