This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGCCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 140.71 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 152,471 (4.8%) Reads that were too short: 17,802 (0.6%) Reads written (passing filters): 134,669 (4.3%) Total basepairs processed: 953,096,634 bp Total written (filtered): 35,799,151 bp (3.8%) === Adapter 1 === Sequence: GTGCCT; Type: regular 5'; Length: 6; Trimmed: 152471 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3872 773.1 0 3872 7 41499 773.1 0 41499 8 34464 773.1 0 34464 9 127 773.1 0 127 10 353 773.1 0 353 11 30 773.1 0 30 12 37 773.1 0 37 13 34 773.1 0 34 14 25 773.1 0 25 15 12 773.1 0 12 16 14 773.1 0 14 17 15 773.1 0 15 18 38 773.1 0 38 19 23 773.1 0 23 20 22 773.1 0 22 21 43 773.1 0 43 22 43 773.1 0 43 23 22 773.1 0 22 24 43 773.1 0 43 25 28 773.1 0 28 26 24 773.1 0 24 27 23 773.1 0 23 28 52 773.1 0 52 29 17 773.1 0 17 30 27 773.1 0 27 31 40 773.1 0 40 32 31 773.1 0 31 33 32 773.1 0 32 34 165 773.1 0 165 35 17 773.1 0 17 36 19 773.1 0 19 37 44 773.1 0 44 38 74 773.1 0 74 39 34 773.1 0 34 40 43 773.1 0 43 41 46 773.1 0 46 42 44 773.1 0 44 43 82 773.1 0 82 44 32 773.1 0 32 45 30 773.1 0 30 46 34 773.1 0 34 47 32 773.1 0 32 48 83 773.1 0 83 49 94 773.1 0 94 50 151 773.1 0 151 51 229 773.1 0 229 52 246 773.1 0 246 53 436 773.1 0 436 54 923 773.1 0 923 55 14809 773.1 0 14809 56 9784 773.1 0 9784 57 11600 773.1 0 11600 58 84 773.1 0 84 59 18 773.1 0 18 60 31 773.1 0 31 61 36 773.1 0 36 62 29 773.1 0 29 63 44 773.1 0 44 64 19 773.1 0 19 65 55 773.1 0 55 66 26 773.1 0 26 67 24 773.1 0 24 68 25 773.1 0 25 69 82 773.1 0 82 70 69 773.1 0 69 71 102 773.1 0 102 72 33 773.1 0 33 73 41 773.1 0 41 74 49 773.1 0 49 75 38 773.1 0 38 76 88 773.1 0 88 77 56 773.1 0 56 78 44 773.1 0 44 79 33 773.1 0 33 80 27 773.1 0 27 81 44 773.1 0 44 82 38 773.1 0 38 83 48 773.1 0 48 84 63 773.1 0 63 85 669 773.1 0 669 86 503 773.1 0 503 87 474 773.1 0 474 88 73 773.1 0 73 89 68 773.1 0 68 90 31 773.1 0 31 91 33 773.1 0 33 92 41 773.1 0 41 93 47 773.1 0 47 94 23 773.1 0 23 95 34 773.1 0 34 96 35 773.1 0 35 97 26 773.1 0 26 98 36 773.1 0 36 99 41 773.1 0 41 100 63 773.1 0 63 101 70 773.1 0 70 102 419 773.1 0 419 103 137 773.1 0 137 104 350 773.1 0 350 105 44 773.1 0 44 106 39 773.1 0 39 107 35 773.1 0 35 108 41 773.1 0 41 109 48 773.1 0 48 110 26 773.1 0 26 111 50 773.1 0 50 112 78 773.1 0 78 113 32 773.1 0 32 114 40 773.1 0 40 115 39 773.1 0 39 116 38 773.1 0 38 117 44 773.1 0 44 118 414 773.1 0 414 119 353 773.1 0 353 120 215 773.1 0 215 121 34 773.1 0 34 122 46 773.1 0 46 123 36 773.1 0 36 124 63 773.1 0 63 125 50 773.1 0 50 126 93 773.1 0 93 127 53 773.1 0 53 128 99 773.1 0 99 129 32 773.1 0 32 130 48 773.1 0 48 131 53 773.1 0 53 132 93 773.1 0 93 133 123 773.1 0 123 134 77 773.1 0 77 135 72 773.1 0 72 136 48 773.1 0 48 137 283 773.1 0 283 138 501 773.1 0 501 139 579 773.1 0 579 140 151 773.1 0 151 141 61 773.1 0 61 142 52 773.1 0 52 143 43 773.1 0 43 144 43 773.1 0 43 145 69 773.1 0 69 146 115 773.1 0 115 147 97 773.1 0 97 148 100 773.1 0 100 149 40 773.1 0 40 150 74 773.1 0 74 151 47 773.1 0 47 152 50 773.1 0 50 153 47 773.1 0 47 154 52 773.1 0 52 155 43 773.1 0 43 156 196 773.1 0 196 157 173 773.1 0 173 158 165 773.1 0 165 159 152 773.1 0 152 160 43 773.1 0 43 161 74 773.1 0 74 162 104 773.1 0 104 163 80 773.1 0 80 164 128 773.1 0 128 165 88 773.1 0 88 166 74 773.1 0 74 167 335 773.1 0 335 168 233 773.1 0 233 169 279 773.1 0 279 170 64 773.1 0 64 171 36 773.1 0 36 172 40 773.1 0 40 173 39 773.1 0 39 174 50 773.1 0 50 175 36 773.1 0 36 176 77 773.1 0 77 177 48 773.1 0 48 178 39 773.1 0 39 179 33 773.1 0 33 180 39 773.1 0 39 181 82 773.1 0 82 182 85 773.1 0 85 183 167 773.1 0 167 184 153 773.1 0 153 185 97 773.1 0 97 186 65 773.1 0 65 187 353 773.1 0 353 188 378 773.1 0 378 189 347 773.1 0 347 190 184 773.1 0 184 191 54 773.1 0 54 192 69 773.1 0 69 193 28 773.1 0 28 194 55 773.1 0 55 195 63 773.1 0 63 196 44 773.1 0 44 197 36 773.1 0 36 198 81 773.1 0 81 199 79 773.1 0 79 200 105 773.1 0 105 201 56 773.1 0 56 202 42 773.1 0 42 203 59 773.1 0 59 204 40 773.1 0 40 205 53 773.1 0 53 206 80 773.1 0 80 207 77 773.1 0 77 208 63 773.1 0 63 209 49 773.1 0 49 210 39 773.1 0 39 211 29 773.1 0 29 212 45 773.1 0 45 213 50 773.1 0 50 214 51 773.1 0 51 215 101 773.1 0 101 216 53 773.1 0 53 217 71 773.1 0 71 218 159 773.1 0 159 219 1921 773.1 0 1921 220 1130 773.1 0 1130 221 1596 773.1 0 1596 222 46 773.1 0 46 223 52 773.1 0 52 224 40 773.1 0 40 225 53 773.1 0 53 226 40 773.1 0 40 227 33 773.1 0 33 228 51 773.1 0 51 229 53 773.1 0 53 230 22 773.1 0 22 231 39 773.1 0 39 232 44 773.1 0 44 233 49 773.1 0 49 234 49 773.1 0 49 235 87 773.1 0 87 236 69 773.1 0 69 237 37 773.1 0 37 238 66 773.1 0 66 239 117 773.1 0 117 240 142 773.1 0 142 241 118 773.1 0 118 242 41 773.1 0 41 243 52 773.1 0 52 244 51 773.1 0 51 245 63 773.1 0 63 246 38 773.1 0 38 247 66 773.1 0 66 248 41 773.1 0 41 249 76 773.1 0 76 250 96 773.1 0 96 251 125 773.1 0 125 252 83 773.1 0 83 253 50 773.1 0 50 254 40 773.1 0 40 255 33 773.1 0 33 256 48 773.1 0 48 257 145 773.1 0 145 258 177 773.1 0 177 259 361 773.1 0 361 260 481 773.1 0 481 261 427 773.1 0 427 262 321 773.1 0 321 263 39 773.1 0 39 264 47 773.1 0 47 265 54 773.1 0 54 266 75 773.1 0 75 267 107 773.1 0 107 268 104 773.1 0 104 269 141 773.1 0 141 270 62 773.1 0 62 271 34 773.1 0 34 272 54 773.1 0 54 273 52 773.1 0 52 274 95 773.1 0 95 275 77 773.1 0 77 276 164 773.1 0 164 277 190 773.1 0 190 278 414 773.1 0 414 279 232 773.1 0 232 280 341 773.1 0 341 281 157 773.1 0 157 282 114 773.1 0 114 283 176 773.1 0 176 284 120 773.1 0 120 285 139 773.1 0 139 286 76 773.1 0 76 287 286 773.1 0 286 288 295 773.1 0 295 289 276 773.1 0 276 290 86 773.1 0 86 291 123 773.1 0 123 292 127 773.1 0 127 293 137 773.1 0 137 294 149 773.1 0 149 295 116 773.1 0 116 296 231 773.1 0 231 297 221 773.1 0 221 298 681 773.1 0 681 299 914 773.1 0 914 300 1196 773.1 0 1196 301 170 773.1 0 170 Finished in 9.08 s (67 us/read; 0.89 M reads/minute). === Summary === Total reads processed: 134,669 Reads with adapters: 41,737 (31.0%) Reads that were too short: 177 (0.1%) Reads written (passing filters): 41,560 (30.9%) Total basepairs processed: 35,799,151 bp Total written (filtered): 11,495,450 bp (32.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 41737 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 14 0.0 3 0 0 9 5 16 179 0.0 3 0 145 19 15 17 41055 0.0 3 38860 1831 287 77 18 85 0.0 3 6 75 4 19 79 0.0 3 2 1 47 29 20 35 0.0 3 0 0 0 35 25 1 0.0 3 0 0 0 1 30 4 0.0 3 0 0 0 4 31 1 0.0 3 1 34 34 0.0 3 0 0 1 33 35 1 0.0 3 0 0 0 1 37 9 0.0 3 0 0 0 9 40 2 0.0 3 1 0 0 1 41 1 0.0 3 0 1 42 1 0.0 3 1 43 1 0.0 3 0 1 45 5 0.0 3 0 0 0 5 46 2 0.0 3 0 0 0 2 49 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 57 3 0.0 3 0 0 0 3 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 64 2 0.0 3 0 0 0 2 66 2 0.0 3 0 0 0 2 67 4 0.0 3 0 0 0 4 70 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 85 2 0.0 3 0 0 0 2 86 1 0.0 3 0 0 0 1 94 3 0.0 3 0 0 0 3 95 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 106 2 0.0 3 0 0 0 2 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 112 2 0.0 3 0 0 0 2 113 1 0.0 3 0 0 0 1 115 2 0.0 3 0 0 0 2 116 2 0.0 3 0 0 0 2 118 3 0.0 3 0 0 0 3 119 2 0.0 3 0 0 1 1 123 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 135 4 0.0 3 0 0 0 4 136 3 0.0 3 0 0 0 3 137 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 6 0.0 3 0 0 0 6 144 1 0.0 3 0 0 0 1 146 10 0.0 3 0 0 1 9 152 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 159 1 0.0 3 0 0 0 1 161 2 0.0 3 0 0 0 2 162 1 0.0 3 0 0 0 1 164 4 0.0 3 0 0 0 4 167 1 0.0 3 0 0 0 1 168 2 0.0 3 0 0 0 2 181 3 0.0 3 0 0 0 3 184 3 0.0 3 0 0 0 3 185 2 0.0 3 0 0 0 2 187 2 0.0 3 0 0 0 2 190 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 194 3 0.0 3 0 0 0 3 195 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 205 4 0.0 3 0 0 0 4 206 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 72 0.0 3 0 0 6 66 220 6 0.0 3 0 0 0 6 221 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 233 6 0.0 3 0 0 0 6 234 4 0.0 3 0 0 0 4 235 2 0.0 3 0 0 0 2 236 1 0.0 3 0 0 0 1 237 2 0.0 3 0 0 0 2 240 1 0.0 3 0 0 0 1 252 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 284 3 0.0 3 0 0 1 2 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.20 s (53 us/read; 1.13 M reads/minute). === Summary === Total reads processed: 41,560 Reads with adapters: 697 (1.7%) Reads written (passing filters): 41,560 (100.0%) Total basepairs processed: 11,495,450 bp Total written (filtered): 11,493,345 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 697 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 35.2% C: 54.1% G: 6.5% T: 4.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 689 649.4 0 689 4 4 162.3 0 4 5 2 40.6 0 2 6 2 10.1 0 2