This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGCCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 138.67 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 149,649 (4.7%) Reads that were too short: 11,079 (0.3%) Reads written (passing filters): 138,570 (4.4%) Total basepairs processed: 953,096,634 bp Total written (filtered): 37,417,236 bp (3.9%) === Adapter 1 === Sequence: GTGCCT; Type: regular 5'; Length: 6; Trimmed: 149649 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4235 773.1 0 4235 7 35473 773.1 0 35473 8 43445 773.1 0 43445 9 112 773.1 0 112 10 32 773.1 0 32 11 28 773.1 0 28 12 10 773.1 0 10 13 16 773.1 0 16 14 10 773.1 0 10 15 10 773.1 0 10 16 11 773.1 0 11 17 28 773.1 0 28 18 43 773.1 0 43 19 21 773.1 0 21 20 18 773.1 0 18 21 26 773.1 0 26 22 18 773.1 0 18 23 17 773.1 0 17 24 28 773.1 0 28 25 27 773.1 0 27 26 18 773.1 0 18 27 41 773.1 0 41 28 75 773.1 0 75 29 15 773.1 0 15 30 20 773.1 0 20 31 23 773.1 0 23 32 12 773.1 0 12 33 18 773.1 0 18 34 164 773.1 0 164 35 15 773.1 0 15 36 12 773.1 0 12 37 37 773.1 0 37 38 85 773.1 0 85 39 18 773.1 0 18 40 44 773.1 0 44 41 44 773.1 0 44 42 39 773.1 0 39 43 81 773.1 0 81 44 43 773.1 0 43 45 33 773.1 0 33 46 23 773.1 0 23 47 43 773.1 0 43 48 74 773.1 0 74 49 99 773.1 0 99 50 120 773.1 0 120 51 215 773.1 0 215 52 231 773.1 0 231 53 476 773.1 0 476 54 915 773.1 0 915 55 17680 773.1 0 17680 56 11705 773.1 0 11705 57 14145 773.1 0 14145 58 64 773.1 0 64 59 19 773.1 0 19 60 18 773.1 0 18 61 24 773.1 0 24 62 30 773.1 0 30 63 28 773.1 0 28 64 12 773.1 0 12 65 25 773.1 0 25 66 19 773.1 0 19 67 12 773.1 0 12 68 17 773.1 0 17 69 43 773.1 0 43 70 34 773.1 0 34 71 46 773.1 0 46 72 27 773.1 0 27 73 27 773.1 0 27 74 23 773.1 0 23 75 23 773.1 0 23 76 36 773.1 0 36 77 37 773.1 0 37 78 27 773.1 0 27 79 17 773.1 0 17 80 17 773.1 0 17 81 29 773.1 0 29 82 35 773.1 0 35 83 35 773.1 0 35 84 46 773.1 0 46 85 602 773.1 0 602 86 553 773.1 0 553 87 404 773.1 0 404 88 29 773.1 0 29 89 20 773.1 0 20 90 20 773.1 0 20 91 18 773.1 0 18 92 21 773.1 0 21 93 36 773.1 0 36 94 18 773.1 0 18 95 15 773.1 0 15 96 20 773.1 0 20 97 19 773.1 0 19 98 20 773.1 0 20 99 21 773.1 0 21 100 32 773.1 0 32 101 30 773.1 0 30 102 113 773.1 0 113 103 83 773.1 0 83 104 184 773.1 0 184 105 28 773.1 0 28 106 28 773.1 0 28 107 24 773.1 0 24 108 33 773.1 0 33 109 30 773.1 0 30 110 21 773.1 0 21 111 15 773.1 0 15 112 66 773.1 0 66 113 22 773.1 0 22 114 14 773.1 0 14 115 12 773.1 0 12 116 22 773.1 0 22 117 29 773.1 0 29 118 267 773.1 0 267 119 196 773.1 0 196 120 73 773.1 0 73 121 17 773.1 0 17 122 26 773.1 0 26 123 20 773.1 0 20 124 25 773.1 0 25 125 13 773.1 0 13 126 53 773.1 0 53 127 28 773.1 0 28 128 74 773.1 0 74 129 37 773.1 0 37 130 34 773.1 0 34 131 29 773.1 0 29 132 44 773.1 0 44 133 25 773.1 0 25 134 27 773.1 0 27 135 29 773.1 0 29 136 34 773.1 0 34 137 122 773.1 0 122 138 279 773.1 0 279 139 284 773.1 0 284 140 83 773.1 0 83 141 55 773.1 0 55 142 26 773.1 0 26 143 32 773.1 0 32 144 19 773.1 0 19 145 34 773.1 0 34 146 71 773.1 0 71 147 52 773.1 0 52 148 43 773.1 0 43 149 20 773.1 0 20 150 56 773.1 0 56 151 28 773.1 0 28 152 21 773.1 0 21 153 31 773.1 0 31 154 27 773.1 0 27 155 31 773.1 0 31 156 211 773.1 0 211 157 80 773.1 0 80 158 75 773.1 0 75 159 78 773.1 0 78 160 17 773.1 0 17 161 39 773.1 0 39 162 44 773.1 0 44 163 53 773.1 0 53 164 50 773.1 0 50 165 32 773.1 0 32 166 37 773.1 0 37 167 95 773.1 0 95 168 104 773.1 0 104 169 143 773.1 0 143 170 24 773.1 0 24 171 11 773.1 0 11 172 25 773.1 0 25 173 32 773.1 0 32 174 31 773.1 0 31 175 25 773.1 0 25 176 62 773.1 0 62 177 18 773.1 0 18 178 18 773.1 0 18 179 14 773.1 0 14 180 18 773.1 0 18 181 29 773.1 0 29 182 35 773.1 0 35 183 100 773.1 0 100 184 72 773.1 0 72 185 72 773.1 0 72 186 29 773.1 0 29 187 277 773.1 0 277 188 102 773.1 0 102 189 137 773.1 0 137 190 89 773.1 0 89 191 41 773.1 0 41 192 54 773.1 0 54 193 14 773.1 0 14 194 35 773.1 0 35 195 31 773.1 0 31 196 11 773.1 0 11 197 19 773.1 0 19 198 44 773.1 0 44 199 64 773.1 0 64 200 40 773.1 0 40 201 31 773.1 0 31 202 32 773.1 0 32 203 30 773.1 0 30 204 18 773.1 0 18 205 30 773.1 0 30 206 35 773.1 0 35 207 35 773.1 0 35 208 29 773.1 0 29 209 59 773.1 0 59 210 25 773.1 0 25 211 13 773.1 0 13 212 21 773.1 0 21 213 19 773.1 0 19 214 22 773.1 0 22 215 51 773.1 0 51 216 31 773.1 0 31 217 52 773.1 0 52 218 63 773.1 0 63 219 982 773.1 0 982 220 643 773.1 0 643 221 804 773.1 0 804 222 14 773.1 0 14 223 10 773.1 0 10 224 18 773.1 0 18 225 22 773.1 0 22 226 36 773.1 0 36 227 21 773.1 0 21 228 27 773.1 0 27 229 25 773.1 0 25 230 18 773.1 0 18 231 31 773.1 0 31 232 27 773.1 0 27 233 20 773.1 0 20 234 20 773.1 0 20 235 30 773.1 0 30 236 29 773.1 0 29 237 19 773.1 0 19 238 17 773.1 0 17 239 40 773.1 0 40 240 64 773.1 0 64 241 67 773.1 0 67 242 22 773.1 0 22 243 31 773.1 0 31 244 17 773.1 0 17 245 18 773.1 0 18 246 21 773.1 0 21 247 41 773.1 0 41 248 19 773.1 0 19 249 36 773.1 0 36 250 70 773.1 0 70 251 39 773.1 0 39 252 27 773.1 0 27 253 16 773.1 0 16 254 19 773.1 0 19 255 18 773.1 0 18 256 21 773.1 0 21 257 65 773.1 0 65 258 89 773.1 0 89 259 139 773.1 0 139 260 228 773.1 0 228 261 154 773.1 0 154 262 124 773.1 0 124 263 19 773.1 0 19 264 22 773.1 0 22 265 36 773.1 0 36 266 33 773.1 0 33 267 40 773.1 0 40 268 34 773.1 0 34 269 38 773.1 0 38 270 27 773.1 0 27 271 19 773.1 0 19 272 22 773.1 0 22 273 24 773.1 0 24 274 45 773.1 0 45 275 66 773.1 0 66 276 191 773.1 0 191 277 273 773.1 0 273 278 498 773.1 0 498 279 181 773.1 0 181 280 217 773.1 0 217 281 65 773.1 0 65 282 56 773.1 0 56 283 78 773.1 0 78 284 52 773.1 0 52 285 78 773.1 0 78 286 35 773.1 0 35 287 106 773.1 0 106 288 120 773.1 0 120 289 157 773.1 0 157 290 35 773.1 0 35 291 80 773.1 0 80 292 71 773.1 0 71 293 69 773.1 0 69 294 118 773.1 0 118 295 59 773.1 0 59 296 91 773.1 0 91 297 112 773.1 0 112 298 761 773.1 0 761 299 939 773.1 0 939 300 1384 773.1 0 1384 301 65 773.1 0 65 Finished in 9.96 s (72 us/read; 0.83 M reads/minute). === Summary === Total reads processed: 138,570 Reads with adapters: 47,851 (34.5%) Reads that were too short: 10 (0.0%) Reads written (passing filters): 47,841 (34.5%) Total basepairs processed: 37,417,236 bp Total written (filtered): 13,050,425 bp (34.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 47851 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 4 0.0 3 0 0 4 19 115 0.0 3 0 109 4 0 2 20 43977 0.0 4 43340 608 25 3 1 21 90 0.0 4 8 74 5 2 1 22 27 0.0 4 3 15 7 1 1 23 3540 0.0 4 3471 45 3 21 24 7 0.0 4 0 5 0 1 1 26 3 0.0 4 0 0 0 1 2 33 1 0.0 4 1 37 1 0.0 4 1 46 1 0.0 4 0 1 50 1 0.0 4 1 51 1 0.0 4 1 55 1 0.0 4 0 0 0 0 1 59 18 0.0 4 0 0 0 0 18 97 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 1 126 1 0.0 4 0 0 0 0 1 127 51 0.0 4 0 0 0 0 51 128 2 0.0 4 0 0 0 0 2 158 1 0.0 4 0 0 0 1 170 1 0.0 4 0 0 0 0 1 194 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 238 1 0.0 4 0 0 0 0 1 282 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.58 s (54 us/read; 1.11 M reads/minute). === Summary === Total reads processed: 47,841 Reads with adapters: 4,032 (8.4%) Reads written (passing filters): 47,841 (100.0%) Total basepairs processed: 13,050,425 bp Total written (filtered): 13,007,739 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4032 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.0% C: 2.9% G: 94.4% T: 1.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 16 747.5 0 16 4 9 186.9 0 9 5 1 46.7 0 1 6 1 11.7 0 1 9 410 0.2 0 13 397 10 1409 0.0 1 44 1365 11 2177 0.0 1 8 2169 12 3 0.0 1 0 3 15 1 0.0 1 0 1 151 3 0.0 1 0 3 170 1 0.0 1 1 190 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.