This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GACGGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 152.19 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 740,358 (23.4%) Reads that were too short: 44,055 (1.4%) Reads written (passing filters): 696,303 (22.0%) Total basepairs processed: 953,096,634 bp Total written (filtered): 148,678,051 bp (15.6%) === Adapter 1 === Sequence: GACGGC; Type: regular 5'; Length: 6; Trimmed: 740358 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3527 773.1 0 3527 7 35361 773.1 0 35361 8 718 773.1 0 718 9 42441 773.1 0 42441 10 135 773.1 0 135 11 50 773.1 0 50 12 58 773.1 0 58 13 44 773.1 0 44 14 59 773.1 0 59 15 88 773.1 0 88 16 101 773.1 0 101 17 95 773.1 0 95 18 69 773.1 0 69 19 122 773.1 0 122 20 122 773.1 0 122 21 85 773.1 0 85 22 105 773.1 0 105 23 76 773.1 0 76 24 83 773.1 0 83 25 73 773.1 0 73 26 65 773.1 0 65 27 76 773.1 0 76 28 74 773.1 0 74 29 67 773.1 0 67 30 90 773.1 0 90 31 57 773.1 0 57 32 73 773.1 0 73 33 76 773.1 0 76 34 106 773.1 0 106 35 136 773.1 0 136 36 99 773.1 0 99 37 72 773.1 0 72 38 68 773.1 0 68 39 75 773.1 0 75 40 89 773.1 0 89 41 224 773.1 0 224 42 230 773.1 0 230 43 191 773.1 0 191 44 99 773.1 0 99 45 174 773.1 0 174 46 123 773.1 0 123 47 156 773.1 0 156 48 102 773.1 0 102 49 448 773.1 0 448 50 522 773.1 0 522 51 921 773.1 0 921 52 86 773.1 0 86 53 157 773.1 0 157 54 99 773.1 0 99 55 131 773.1 0 131 56 92 773.1 0 92 57 89 773.1 0 89 58 130 773.1 0 130 59 261 773.1 0 261 60 930 773.1 0 930 61 1535 773.1 0 1535 62 1209 773.1 0 1209 63 176 773.1 0 176 64 116 773.1 0 116 65 183 773.1 0 183 66 136 773.1 0 136 67 308 773.1 0 308 68 551 773.1 0 551 69 719 773.1 0 719 70 1061 773.1 0 1061 71 1964 773.1 0 1964 72 3163 773.1 0 3163 73 792 773.1 0 792 74 661 773.1 0 661 75 1326 773.1 0 1326 76 529 773.1 0 529 77 640 773.1 0 640 78 1075 773.1 0 1075 79 1079 773.1 0 1079 80 1591 773.1 0 1591 81 1565 773.1 0 1565 82 3011 773.1 0 3011 83 4073 773.1 0 4073 84 6008 773.1 0 6008 85 176286 773.1 0 176286 86 126760 773.1 0 126760 87 173341 773.1 0 173341 88 684 773.1 0 684 89 73 773.1 0 73 90 71 773.1 0 71 91 83 773.1 0 83 92 45 773.1 0 45 93 59 773.1 0 59 94 63 773.1 0 63 95 55 773.1 0 55 96 59 773.1 0 59 97 56 773.1 0 56 98 55 773.1 0 55 99 49 773.1 0 49 100 47 773.1 0 47 101 42 773.1 0 42 102 58 773.1 0 58 103 38 773.1 0 38 104 38 773.1 0 38 105 40 773.1 0 40 106 47 773.1 0 47 107 63 773.1 0 63 108 58 773.1 0 58 109 64 773.1 0 64 110 51 773.1 0 51 111 61 773.1 0 61 112 50 773.1 0 50 113 63 773.1 0 63 114 50 773.1 0 50 115 64 773.1 0 64 116 50 773.1 0 50 117 61 773.1 0 61 118 54 773.1 0 54 119 51 773.1 0 51 120 49 773.1 0 49 121 77 773.1 0 77 122 143 773.1 0 143 123 154 773.1 0 154 124 183 773.1 0 183 125 75 773.1 0 75 126 65 773.1 0 65 127 77 773.1 0 77 128 118 773.1 0 118 129 120 773.1 0 120 130 313 773.1 0 313 131 345 773.1 0 345 132 236 773.1 0 236 133 221 773.1 0 221 134 203 773.1 0 203 135 209 773.1 0 209 136 115 773.1 0 115 137 85 773.1 0 85 138 68 773.1 0 68 139 91 773.1 0 91 140 99 773.1 0 99 141 83 773.1 0 83 142 102 773.1 0 102 143 119 773.1 0 119 144 81 773.1 0 81 145 86 773.1 0 86 146 100 773.1 0 100 147 151 773.1 0 151 148 181 773.1 0 181 149 1298 773.1 0 1298 150 847 773.1 0 847 151 974 773.1 0 974 152 1641 773.1 0 1641 153 2309 773.1 0 2309 154 1974 773.1 0 1974 155 1467 773.1 0 1467 156 338 773.1 0 338 157 100 773.1 0 100 158 93 773.1 0 93 159 127 773.1 0 127 160 166 773.1 0 166 161 169 773.1 0 169 162 312 773.1 0 312 163 129 773.1 0 129 164 216 773.1 0 216 165 447 773.1 0 447 166 206 773.1 0 206 167 183 773.1 0 183 168 482 773.1 0 482 169 343 773.1 0 343 170 578 773.1 0 578 171 483 773.1 0 483 172 569 773.1 0 569 173 772 773.1 0 772 174 1253 773.1 0 1253 175 22991 773.1 0 22991 176 19888 773.1 0 19888 177 27630 773.1 0 27630 178 142 773.1 0 142 179 55 773.1 0 55 180 69 773.1 0 69 181 174 773.1 0 174 182 162 773.1 0 162 183 161 773.1 0 161 184 69 773.1 0 69 185 77 773.1 0 77 186 124 773.1 0 124 187 76 773.1 0 76 188 62 773.1 0 62 189 89 773.1 0 89 190 111 773.1 0 111 191 106 773.1 0 106 192 91 773.1 0 91 193 81 773.1 0 81 194 80 773.1 0 80 195 92 773.1 0 92 196 89 773.1 0 89 197 74 773.1 0 74 198 84 773.1 0 84 199 79 773.1 0 79 200 62 773.1 0 62 201 100 773.1 0 100 202 65 773.1 0 65 203 85 773.1 0 85 204 110 773.1 0 110 205 109 773.1 0 109 206 157 773.1 0 157 207 94 773.1 0 94 208 126 773.1 0 126 209 160 773.1 0 160 210 207 773.1 0 207 211 251 773.1 0 251 212 352 773.1 0 352 213 8400 773.1 0 8400 214 3597 773.1 0 3597 215 6749 773.1 0 6749 216 130 773.1 0 130 217 205 773.1 0 205 218 320 773.1 0 320 219 232 773.1 0 232 220 173 773.1 0 173 221 139 773.1 0 139 222 104 773.1 0 104 223 61 773.1 0 61 224 79 773.1 0 79 225 80 773.1 0 80 226 90 773.1 0 90 227 162 773.1 0 162 228 379 773.1 0 379 229 296 773.1 0 296 230 403 773.1 0 403 231 1345 773.1 0 1345 232 537 773.1 0 537 233 902 773.1 0 902 234 140 773.1 0 140 235 148 773.1 0 148 236 125 773.1 0 125 237 92 773.1 0 92 238 114 773.1 0 114 239 82 773.1 0 82 240 101 773.1 0 101 241 100 773.1 0 100 242 86 773.1 0 86 243 97 773.1 0 97 244 82 773.1 0 82 245 87 773.1 0 87 246 110 773.1 0 110 247 156 773.1 0 156 248 201 773.1 0 201 249 242 773.1 0 242 250 217 773.1 0 217 251 196 773.1 0 196 252 147 773.1 0 147 253 585 773.1 0 585 254 853 773.1 0 853 255 743 773.1 0 743 256 497 773.1 0 497 257 398 773.1 0 398 258 339 773.1 0 339 259 204 773.1 0 204 260 145 773.1 0 145 261 130 773.1 0 130 262 168 773.1 0 168 263 74 773.1 0 74 264 83 773.1 0 83 265 69 773.1 0 69 266 114 773.1 0 114 267 116 773.1 0 116 268 116 773.1 0 116 269 109 773.1 0 109 270 126 773.1 0 126 271 132 773.1 0 132 272 106 773.1 0 106 273 68 773.1 0 68 274 109 773.1 0 109 275 127 773.1 0 127 276 245 773.1 0 245 277 533 773.1 0 533 278 706 773.1 0 706 279 838 773.1 0 838 280 160 773.1 0 160 281 148 773.1 0 148 282 152 773.1 0 152 283 123 773.1 0 123 284 103 773.1 0 103 285 501 773.1 0 501 286 543 773.1 0 543 287 475 773.1 0 475 288 231 773.1 0 231 289 188 773.1 0 188 290 223 773.1 0 223 291 158 773.1 0 158 292 176 773.1 0 176 293 362 773.1 0 362 294 334 773.1 0 334 295 335 773.1 0 335 296 330 773.1 0 330 297 729 773.1 0 729 298 830 773.1 0 830 299 730 773.1 0 730 300 476 773.1 0 476 301 393 773.1 0 393 Finished in 39.20 s (56 us/read; 1.07 M reads/minute). === Summary === Total reads processed: 696,303 Reads with adapters: 61,094 (8.8%) Reads that were too short: 2,192 (0.3%) Reads written (passing filters): 58,902 (8.5%) Total basepairs processed: 148,678,051 bp Total written (filtered): 14,437,953 bp (9.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 61094 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 8 0.0 2 0 0 0 8 15 18 0.0 3 0 0 6 12 16 214 0.0 3 0 171 26 17 17 43756 0.0 3 40130 2260 409 957 18 133 0.0 3 5 92 20 16 19 3531 0.0 3 3257 150 75 49 20 185 0.0 3 6 12 2 165 21 10 0.0 3 0 0 5 5 22 5 0.0 3 0 0 0 5 23 1 0.0 3 0 0 0 1 26 1 0.0 3 1 31 1 0.0 3 0 0 0 1 33 2 0.0 3 0 0 0 2 35 4 0.0 3 0 0 0 4 36 5 0.0 3 0 0 0 5 37 718 0.0 3 0 0 11 707 38 115 0.0 3 0 0 1 114 39 5 0.0 3 0 0 0 5 41 24 0.0 3 0 0 1 23 42 1 0.0 3 0 0 0 1 43 3 0.0 3 0 0 0 3 44 1 0.0 3 1 48 1 0.0 3 0 0 0 1 49 3 0.0 3 0 0 0 3 50 2 0.0 3 0 0 0 2 51 13 0.0 3 0 0 1 12 52 6 0.0 3 0 0 1 5 53 15 0.0 3 0 0 0 15 55 2 0.0 3 0 0 0 2 56 8 0.0 3 0 0 0 8 57 1 0.0 3 0 0 0 1 58 2 0.0 3 0 0 0 2 59 3 0.0 3 0 0 0 3 60 2 0.0 3 0 0 0 2 62 4 0.0 3 0 0 0 4 63 5 0.0 3 0 0 0 5 64 11 0.0 3 0 0 0 11 65 40 0.0 3 0 0 0 40 66 1 0.0 3 0 0 0 1 67 5 0.0 3 0 0 0 5 68 16 0.0 3 0 0 1 15 69 4 0.0 3 0 0 0 4 70 4 0.0 3 0 0 0 4 71 1 0.0 3 0 0 0 1 72 2 0.0 3 0 0 0 2 77 14 0.0 3 0 0 0 14 79 2 0.0 3 0 0 0 2 80 1 0.0 3 0 0 0 1 81 27 0.0 3 0 0 0 27 82 3 0.0 3 0 0 0 3 83 6 0.0 3 0 0 0 6 84 9 0.0 3 0 0 1 8 85 13 0.0 3 0 0 0 13 86 6384 0.0 3 0 0 83 6301 87 788 0.0 3 0 0 5 783 88 165 0.0 3 0 0 2 163 89 867 0.0 3 0 0 44 823 90 145 0.0 3 0 0 4 141 91 49 0.0 3 0 0 6 43 92 18 0.0 3 0 0 0 18 93 6 0.0 3 0 0 0 6 94 70 0.0 3 0 0 1 69 95 4 0.0 3 0 0 1 3 96 8 0.0 3 0 0 0 8 97 17 0.0 3 0 0 1 16 98 13 0.0 3 0 0 0 13 99 5 0.0 3 0 0 0 5 100 495 0.0 3 0 0 6 489 101 22 0.0 3 0 0 0 22 102 38 0.0 3 0 0 1 37 103 84 0.0 3 0 0 5 79 104 8 0.0 3 0 0 0 8 105 11 0.0 3 0 0 0 11 106 100 0.0 3 0 0 0 100 107 265 0.0 3 0 0 2 263 108 6 0.0 3 0 0 0 6 109 28 0.0 3 0 0 0 28 110 62 0.0 3 0 0 1 61 111 8 0.0 3 0 0 0 8 112 142 0.0 3 0 0 2 140 113 26 0.0 3 0 0 0 26 114 8 0.0 3 0 0 0 8 115 3 0.0 3 0 0 0 3 116 2 0.0 3 0 0 0 2 117 1 0.0 3 0 0 0 1 118 11 0.0 3 0 0 0 11 119 6 0.0 3 0 0 0 6 120 2 0.0 3 0 0 0 2 121 3 0.0 3 0 0 0 3 123 1 0.0 3 0 0 0 1 124 21 0.0 3 0 0 0 21 125 5 0.0 3 0 0 0 5 126 4 0.0 3 0 0 0 4 127 2 0.0 3 0 0 0 2 128 3 0.0 3 0 0 0 3 129 1 0.0 3 0 0 0 1 130 5 0.0 3 0 0 0 5 131 74 0.0 3 0 0 0 74 132 15 0.0 3 0 0 0 15 133 2 0.0 3 0 0 0 2 134 11 0.0 3 0 0 0 11 135 3 0.0 3 0 0 0 3 137 78 0.0 3 0 0 3 75 138 34 0.0 3 0 0 1 33 139 41 0.0 3 0 0 2 39 140 5 0.0 3 0 0 0 5 141 11 0.0 3 0 0 0 11 142 2 0.0 3 0 0 0 2 143 30 0.0 3 0 0 0 30 144 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 146 10 0.0 3 0 0 0 10 147 9 0.0 3 0 0 0 9 148 2 0.0 3 0 0 0 2 149 7 0.0 3 0 0 0 7 150 1 0.0 3 0 0 0 1 151 8 0.0 3 0 0 0 8 152 3 0.0 3 0 0 0 3 153 4 0.0 3 0 0 0 4 154 2 0.0 3 0 0 0 2 155 2 0.0 3 0 0 0 2 156 3 0.0 3 0 0 0 3 157 14 0.0 3 0 0 0 14 158 45 0.0 3 0 0 0 45 159 2 0.0 3 0 0 0 2 160 2 0.0 3 0 0 0 2 161 3 0.0 3 0 0 0 3 162 1 0.0 3 0 0 0 1 163 3 0.0 3 0 0 0 3 164 5 0.0 3 0 0 0 5 165 2 0.0 3 0 0 1 1 166 6 0.0 3 0 0 0 6 167 8 0.0 3 0 0 0 8 168 2 0.0 3 0 0 0 2 169 1 0.0 3 0 0 0 1 170 3 0.0 3 0 0 1 2 171 4 0.0 3 0 0 0 4 172 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 174 2 0.0 3 0 0 0 2 176 5 0.0 3 0 0 0 5 177 4 0.0 3 0 0 0 4 178 5 0.0 3 0 0 0 5 179 4 0.0 3 0 0 0 4 180 3 0.0 3 0 0 0 3 181 2 0.0 3 0 0 0 2 182 7 0.0 3 0 0 0 7 183 4 0.0 3 0 0 1 3 184 2 0.0 3 0 0 0 2 185 3 0.0 3 0 0 1 2 186 11 0.0 3 0 0 0 11 187 21 0.0 3 0 0 1 20 188 53 0.0 3 0 0 4 49 189 75 0.0 3 0 0 1 74 190 6 0.0 3 0 0 0 6 191 15 0.0 3 0 0 0 15 192 1 0.0 3 0 0 0 1 194 8 0.0 3 0 0 1 7 195 2 0.0 3 0 0 0 2 196 1 0.0 3 0 0 0 1 197 4 0.0 3 0 0 0 4 198 303 0.0 3 0 0 3 300 199 146 0.0 3 0 0 3 143 200 88 0.0 3 0 0 0 88 201 172 0.0 3 0 0 4 168 202 150 0.0 3 0 0 10 140 203 40 0.0 3 0 0 1 39 204 14 0.0 3 0 0 1 13 205 10 0.0 3 0 0 0 10 206 61 0.0 3 0 0 1 60 207 60 0.0 3 0 0 1 59 208 21 0.0 3 0 0 0 21 209 17 0.0 3 0 0 1 16 210 1 0.0 3 0 0 0 1 211 14 0.0 3 0 0 0 14 212 53 0.0 3 0 0 3 50 213 13 0.0 3 0 0 0 13 214 37 0.0 3 0 0 2 35 215 50 0.0 3 0 0 0 50 216 190 0.0 3 0 0 5 185 217 5 0.0 3 0 0 0 5 218 7 0.0 3 0 0 0 7 219 1 0.0 3 0 0 0 1 221 3 0.0 3 0 0 0 3 222 2 0.0 3 0 0 0 2 225 1 0.0 3 0 0 0 1 226 23 0.0 3 0 0 0 23 229 2 0.0 3 0 0 0 2 232 3 0.0 3 0 0 0 3 234 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 244 2 0.0 3 0 0 0 2 246 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 265 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 3 0.0 3 0 0 1 2 281 3 0.0 3 0 0 0 3 282 3 0.0 3 0 0 0 3 283 4 0.0 3 0 0 0 4 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.15 s (53 us/read; 1.12 M reads/minute). === Summary === Total reads processed: 58,902 Reads with adapters: 2,269 (3.9%) Reads written (passing filters): 58,902 (100.0%) Total basepairs processed: 14,437,953 bp Total written (filtered): 14,431,089 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2269 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 66.8% C: 14.5% G: 16.7% T: 1.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2228 920.3 0 2228 4 34 230.1 0 34 5 5 57.5 0 5 6 1 14.4 0 1 13 1 0.0 1 0 1