This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 145.17 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 157,358 (5.0%) Reads that were too short: 7,779 (0.2%) Reads written (passing filters): 149,579 (4.7%) Total basepairs processed: 953,096,634 bp Total written (filtered): 42,677,008 bp (4.5%) === Adapter 1 === Sequence: GTGATC; Type: regular 5'; Length: 6; Trimmed: 157358 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4340 773.1 0 4340 7 4928 773.1 0 4928 8 75520 773.1 0 75520 9 54082 773.1 0 54082 10 251 773.1 0 251 11 9 773.1 0 9 12 3 773.1 0 3 13 4 773.1 0 4 14 1 773.1 0 1 16 3 773.1 0 3 17 2 773.1 0 2 18 3 773.1 0 3 19 6 773.1 0 6 20 3 773.1 0 3 21 3 773.1 0 3 22 8 773.1 0 8 23 22 773.1 0 22 24 6 773.1 0 6 25 10 773.1 0 10 26 2 773.1 0 2 27 2 773.1 0 2 28 2 773.1 0 2 29 17 773.1 0 17 30 18 773.1 0 18 31 8 773.1 0 8 32 6 773.1 0 6 33 95 773.1 0 95 34 36 773.1 0 36 35 15 773.1 0 15 36 43 773.1 0 43 37 10 773.1 0 10 38 11 773.1 0 11 39 32 773.1 0 32 40 50 773.1 0 50 41 3 773.1 0 3 42 9 773.1 0 9 43 4 773.1 0 4 44 7 773.1 0 7 45 10 773.1 0 10 46 10 773.1 0 10 47 3 773.1 0 3 48 10 773.1 0 10 49 5 773.1 0 5 50 6 773.1 0 6 51 7 773.1 0 7 52 8 773.1 0 8 53 11 773.1 0 11 54 13 773.1 0 13 55 6 773.1 0 6 56 4 773.1 0 4 57 6 773.1 0 6 58 71 773.1 0 71 59 182 773.1 0 182 60 109 773.1 0 109 61 4 773.1 0 4 62 5 773.1 0 5 63 7 773.1 0 7 64 7 773.1 0 7 65 3 773.1 0 3 66 5 773.1 0 5 67 6 773.1 0 6 68 3 773.1 0 3 69 9 773.1 0 9 70 22 773.1 0 22 71 45 773.1 0 45 72 13 773.1 0 13 73 26 773.1 0 26 74 15 773.1 0 15 75 13 773.1 0 13 76 103 773.1 0 103 77 238 773.1 0 238 78 129 773.1 0 129 79 16 773.1 0 16 80 172 773.1 0 172 81 162 773.1 0 162 82 135 773.1 0 135 83 10 773.1 0 10 84 6 773.1 0 6 85 25 773.1 0 25 86 7 773.1 0 7 87 16 773.1 0 16 88 23 773.1 0 23 89 15 773.1 0 15 90 15 773.1 0 15 91 12 773.1 0 12 92 5 773.1 0 5 93 17 773.1 0 17 94 18 773.1 0 18 95 17 773.1 0 17 96 9 773.1 0 9 97 16 773.1 0 16 98 15 773.1 0 15 99 33 773.1 0 33 100 591 773.1 0 591 101 992 773.1 0 992 102 843 773.1 0 843 103 12 773.1 0 12 104 13 773.1 0 13 105 15 773.1 0 15 106 16 773.1 0 16 107 9 773.1 0 9 108 28 773.1 0 28 109 110 773.1 0 110 110 1360 773.1 0 1360 111 867 773.1 0 867 112 550 773.1 0 550 113 11 773.1 0 11 114 4 773.1 0 4 115 11 773.1 0 11 116 26 773.1 0 26 117 3 773.1 0 3 118 6 773.1 0 6 119 3 773.1 0 3 120 4 773.1 0 4 121 12 773.1 0 12 122 6 773.1 0 6 123 8 773.1 0 8 124 8 773.1 0 8 125 12 773.1 0 12 126 13 773.1 0 13 127 34 773.1 0 34 128 55 773.1 0 55 129 39 773.1 0 39 130 88 773.1 0 88 131 17 773.1 0 17 132 33 773.1 0 33 133 20 773.1 0 20 134 9 773.1 0 9 135 13 773.1 0 13 136 12 773.1 0 12 137 7 773.1 0 7 138 16 773.1 0 16 139 2 773.1 0 2 140 13 773.1 0 13 141 10 773.1 0 10 142 23 773.1 0 23 143 11 773.1 0 11 144 14 773.1 0 14 145 45 773.1 0 45 146 11 773.1 0 11 147 86 773.1 0 86 148 55 773.1 0 55 149 46 773.1 0 46 150 53 773.1 0 53 151 11 773.1 0 11 152 28 773.1 0 28 153 22 773.1 0 22 154 20 773.1 0 20 155 7 773.1 0 7 156 20 773.1 0 20 157 40 773.1 0 40 158 32 773.1 0 32 159 20 773.1 0 20 160 18 773.1 0 18 161 21 773.1 0 21 162 17 773.1 0 17 163 11 773.1 0 11 164 13 773.1 0 13 165 5 773.1 0 5 166 12 773.1 0 12 167 15 773.1 0 15 168 65 773.1 0 65 169 97 773.1 0 97 170 89 773.1 0 89 171 6 773.1 0 6 172 9 773.1 0 9 173 9 773.1 0 9 174 6 773.1 0 6 175 8 773.1 0 8 176 9 773.1 0 9 177 31 773.1 0 31 178 14 773.1 0 14 179 15 773.1 0 15 180 17 773.1 0 17 181 17 773.1 0 17 182 37 773.1 0 37 183 47 773.1 0 47 184 79 773.1 0 79 185 32 773.1 0 32 186 17 773.1 0 17 187 17 773.1 0 17 188 18 773.1 0 18 189 40 773.1 0 40 190 11 773.1 0 11 191 12 773.1 0 12 192 14 773.1 0 14 193 15 773.1 0 15 194 26 773.1 0 26 195 28 773.1 0 28 196 43 773.1 0 43 197 107 773.1 0 107 198 99 773.1 0 99 199 244 773.1 0 244 200 177 773.1 0 177 201 135 773.1 0 135 202 362 773.1 0 362 203 254 773.1 0 254 204 618 773.1 0 618 205 63 773.1 0 63 206 41 773.1 0 41 207 18 773.1 0 18 208 28 773.1 0 28 209 30 773.1 0 30 210 30 773.1 0 30 211 31 773.1 0 31 212 22 773.1 0 22 213 22 773.1 0 22 214 39 773.1 0 39 215 24 773.1 0 24 216 19 773.1 0 19 217 103 773.1 0 103 218 121 773.1 0 121 219 202 773.1 0 202 220 52 773.1 0 52 221 32 773.1 0 32 222 354 773.1 0 354 223 485 773.1 0 485 224 431 773.1 0 431 225 82 773.1 0 82 226 25 773.1 0 25 227 36 773.1 0 36 228 32 773.1 0 32 229 57 773.1 0 57 230 24 773.1 0 24 231 15 773.1 0 15 232 13 773.1 0 13 233 19 773.1 0 19 234 33 773.1 0 33 235 25 773.1 0 25 236 27 773.1 0 27 237 129 773.1 0 129 238 28 773.1 0 28 239 39 773.1 0 39 240 23 773.1 0 23 241 60 773.1 0 60 242 46 773.1 0 46 243 72 773.1 0 72 244 34 773.1 0 34 245 26 773.1 0 26 246 16 773.1 0 16 247 24 773.1 0 24 248 23 773.1 0 23 249 32 773.1 0 32 250 39 773.1 0 39 251 39 773.1 0 39 252 47 773.1 0 47 253 34 773.1 0 34 254 53 773.1 0 53 255 83 773.1 0 83 256 159 773.1 0 159 257 167 773.1 0 167 258 251 773.1 0 251 259 93 773.1 0 93 260 82 773.1 0 82 261 37 773.1 0 37 262 56 773.1 0 56 263 46 773.1 0 46 264 48 773.1 0 48 265 35 773.1 0 35 266 24 773.1 0 24 267 31 773.1 0 31 268 29 773.1 0 29 269 28 773.1 0 28 270 39 773.1 0 39 271 58 773.1 0 58 272 47 773.1 0 47 273 50 773.1 0 50 274 26 773.1 0 26 275 41 773.1 0 41 276 55 773.1 0 55 277 66 773.1 0 66 278 93 773.1 0 93 279 56 773.1 0 56 280 61 773.1 0 61 281 34 773.1 0 34 282 38 773.1 0 38 283 53 773.1 0 53 284 37 773.1 0 37 285 59 773.1 0 59 286 36 773.1 0 36 287 51 773.1 0 51 288 47 773.1 0 47 289 87 773.1 0 87 290 109 773.1 0 109 291 139 773.1 0 139 292 106 773.1 0 106 293 62 773.1 0 62 294 47 773.1 0 47 295 82 773.1 0 82 296 101 773.1 0 101 297 54 773.1 0 54 298 96 773.1 0 96 299 80 773.1 0 80 300 104 773.1 0 104 301 63 773.1 0 63 Finished in 11.06 s (74 us/read; 0.81 M reads/minute). === Summary === Total reads processed: 149,579 Reads with adapters: 79,218 (53.0%) Reads that were too short: 114 (0.1%) Reads written (passing filters): 79,104 (52.9%) Total basepairs processed: 42,677,008 bp Total written (filtered): 21,818,449 bp (51.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 79218 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 10 0.0 2 0 0 0 10 15 33 0.0 3 0 0 22 11 16 486 0.0 3 0 400 55 31 17 74521 0.0 3 70549 3302 542 128 18 3588 0.0 3 3249 283 40 16 19 228 0.0 3 18 8 131 71 20 92 0.0 3 2 1 2 87 21 15 0.0 3 0 0 0 15 23 1 0.0 3 0 0 1 24 2 0.0 3 0 1 1 25 1 0.0 3 0 0 0 1 27 1 0.0 3 1 31 1 0.0 3 0 1 32 2 0.0 3 0 0 0 2 34 2 0.0 3 0 0 0 2 35 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 42 2 0.0 3 0 0 0 2 43 5 0.0 3 0 0 0 5 44 1 0.0 3 0 0 0 1 46 2 0.0 3 0 0 0 2 48 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 52 1 0.0 3 0 0 1 57 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 68 2 0.0 3 0 0 0 2 75 2 0.0 3 1 0 1 76 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 82 47 0.0 3 0 0 1 46 84 1 0.0 3 0 0 0 1 85 10 0.0 3 0 0 0 10 86 2 0.0 3 0 0 0 2 88 1 0.0 3 0 0 0 1 90 2 0.0 3 0 0 0 2 91 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 0 1 101 3 0.0 3 0 0 0 3 104 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 5 0.0 3 0 0 0 5 112 3 0.0 3 0 0 0 3 114 1 0.0 3 0 0 0 1 118 2 0.0 3 0 0 0 2 123 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 133 2 0.0 3 0 0 0 2 134 1 0.0 3 0 0 0 1 137 2 0.0 3 0 0 0 2 138 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 159 2 0.0 3 0 0 0 2 161 1 0.0 3 0 0 0 1 163 4 0.0 3 0 0 0 4 164 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 1 183 3 0.0 3 0 0 0 3 186 1 0.0 3 0 0 0 1 187 3 0.0 3 0 0 0 3 189 4 0.0 3 0 0 0 4 192 2 0.0 3 0 0 0 2 194 3 0.0 3 0 0 0 3 195 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 205 3 0.0 3 0 0 0 3 206 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 217 53 0.0 3 0 0 1 52 218 4 0.0 3 0 0 0 4 222 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 3 0.0 3 0 0 0 3 280 4 0.0 3 0 0 0 4 281 3 0.0 3 0 0 0 3 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.33 s (55 us/read; 1.10 M reads/minute). === Summary === Total reads processed: 79,104 Reads with adapters: 511 (0.6%) Reads written (passing filters): 79,104 (100.0%) Total basepairs processed: 21,818,449 bp Total written (filtered): 21,816,665 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 511 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 28.2% C: 8.8% G: 58.5% T: 4.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 275 1236.0 0 275 4 223 309.0 0 223 5 12 77.2 0 12 7 1 4.8 0 1