This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 146.11 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 153,527 (4.8%) Reads that were too short: 2,465 (0.1%) Reads written (passing filters): 151,062 (4.8%) Total basepairs processed: 953,096,634 bp Total written (filtered): 43,526,838 bp (4.6%) === Adapter 1 === Sequence: GTGATC; Type: regular 5'; Length: 6; Trimmed: 153527 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3846 773.1 0 3846 7 5207 773.1 0 5207 8 55411 773.1 0 55411 9 79925 773.1 0 79925 10 143 773.1 0 143 11 6 773.1 0 6 12 4 773.1 0 4 13 2 773.1 0 2 14 1 773.1 0 1 15 1 773.1 0 1 16 1 773.1 0 1 17 1 773.1 0 1 18 3 773.1 0 3 19 3 773.1 0 3 20 3 773.1 0 3 22 1 773.1 0 1 23 4 773.1 0 4 24 1 773.1 0 1 25 2 773.1 0 2 26 2 773.1 0 2 27 2 773.1 0 2 29 7 773.1 0 7 30 14 773.1 0 14 31 4 773.1 0 4 32 3 773.1 0 3 33 7 773.1 0 7 34 9 773.1 0 9 35 8 773.1 0 8 36 35 773.1 0 35 37 2 773.1 0 2 38 18 773.1 0 18 39 36 773.1 0 36 40 13 773.1 0 13 41 5 773.1 0 5 42 3 773.1 0 3 43 8 773.1 0 8 44 2 773.1 0 2 45 3 773.1 0 3 46 17 773.1 0 17 47 4 773.1 0 4 48 3 773.1 0 3 49 2 773.1 0 2 50 3 773.1 0 3 51 2 773.1 0 2 52 2 773.1 0 2 53 2 773.1 0 2 54 9 773.1 0 9 55 6 773.1 0 6 56 2 773.1 0 2 57 4 773.1 0 4 58 25 773.1 0 25 59 129 773.1 0 129 60 50 773.1 0 50 61 1 773.1 0 1 62 3 773.1 0 3 63 6 773.1 0 6 64 3 773.1 0 3 65 6 773.1 0 6 66 6 773.1 0 6 67 5 773.1 0 5 68 6 773.1 0 6 69 9 773.1 0 9 70 12 773.1 0 12 71 34 773.1 0 34 72 8 773.1 0 8 73 13 773.1 0 13 74 3 773.1 0 3 75 4 773.1 0 4 76 79 773.1 0 79 77 204 773.1 0 204 78 81 773.1 0 81 79 2 773.1 0 2 80 86 773.1 0 86 81 75 773.1 0 75 82 24 773.1 0 24 83 8 773.1 0 8 84 1 773.1 0 1 85 3 773.1 0 3 86 5 773.1 0 5 87 3 773.1 0 3 88 2 773.1 0 2 89 7 773.1 0 7 90 4 773.1 0 4 91 4 773.1 0 4 92 5 773.1 0 5 93 4 773.1 0 4 94 13 773.1 0 13 95 13 773.1 0 13 96 6 773.1 0 6 97 12 773.1 0 12 98 5 773.1 0 5 99 21 773.1 0 21 100 277 773.1 0 277 101 476 773.1 0 476 102 456 773.1 0 456 103 8 773.1 0 8 104 9 773.1 0 9 105 13 773.1 0 13 106 16 773.1 0 16 107 18 773.1 0 18 108 19 773.1 0 19 109 117 773.1 0 117 110 1208 773.1 0 1208 111 841 773.1 0 841 112 512 773.1 0 512 113 8 773.1 0 8 114 3 773.1 0 3 115 7 773.1 0 7 116 20 773.1 0 20 117 7 773.1 0 7 118 2 773.1 0 2 119 2 773.1 0 2 120 3 773.1 0 3 121 2 773.1 0 2 122 3 773.1 0 3 123 2 773.1 0 2 124 7 773.1 0 7 125 6 773.1 0 6 126 16 773.1 0 16 127 29 773.1 0 29 128 64 773.1 0 64 129 46 773.1 0 46 130 49 773.1 0 49 131 3 773.1 0 3 132 4 773.1 0 4 133 7 773.1 0 7 134 11 773.1 0 11 135 14 773.1 0 14 136 3 773.1 0 3 137 13 773.1 0 13 138 9 773.1 0 9 139 3 773.1 0 3 140 6 773.1 0 6 141 6 773.1 0 6 142 5 773.1 0 5 143 8 773.1 0 8 144 7 773.1 0 7 145 38 773.1 0 38 146 5 773.1 0 5 147 60 773.1 0 60 148 21 773.1 0 21 149 19 773.1 0 19 150 13 773.1 0 13 151 6 773.1 0 6 152 17 773.1 0 17 153 32 773.1 0 32 154 13 773.1 0 13 155 6 773.1 0 6 156 4 773.1 0 4 157 8 773.1 0 8 158 3 773.1 0 3 159 8 773.1 0 8 160 7 773.1 0 7 161 6 773.1 0 6 162 7 773.1 0 7 163 6 773.1 0 6 164 2 773.1 0 2 165 3 773.1 0 3 166 6 773.1 0 6 167 12 773.1 0 12 168 76 773.1 0 76 169 106 773.1 0 106 170 85 773.1 0 85 171 4 773.1 0 4 172 4 773.1 0 4 173 2 773.1 0 2 174 2 773.1 0 2 175 2 773.1 0 2 176 7 773.1 0 7 177 7 773.1 0 7 178 4 773.1 0 4 179 7 773.1 0 7 180 7 773.1 0 7 181 6 773.1 0 6 182 6 773.1 0 6 183 12 773.1 0 12 184 20 773.1 0 20 185 14 773.1 0 14 186 6 773.1 0 6 187 5 773.1 0 5 188 10 773.1 0 10 189 4 773.1 0 4 190 11 773.1 0 11 191 10 773.1 0 10 192 3 773.1 0 3 193 10 773.1 0 10 194 10 773.1 0 10 195 7 773.1 0 7 196 15 773.1 0 15 197 28 773.1 0 28 198 21 773.1 0 21 199 51 773.1 0 51 200 38 773.1 0 38 201 23 773.1 0 23 202 70 773.1 0 70 203 38 773.1 0 38 204 90 773.1 0 90 205 16 773.1 0 16 206 11 773.1 0 11 207 5 773.1 0 5 208 10 773.1 0 10 209 10 773.1 0 10 210 5 773.1 0 5 211 9 773.1 0 9 212 7 773.1 0 7 213 6 773.1 0 6 214 11 773.1 0 11 215 10 773.1 0 10 216 10 773.1 0 10 217 34 773.1 0 34 218 31 773.1 0 31 219 75 773.1 0 75 220 23 773.1 0 23 221 6 773.1 0 6 222 85 773.1 0 85 223 113 773.1 0 113 224 92 773.1 0 92 225 21 773.1 0 21 226 10 773.1 0 10 227 15 773.1 0 15 228 22 773.1 0 22 229 22 773.1 0 22 230 6 773.1 0 6 231 6 773.1 0 6 232 9 773.1 0 9 233 7 773.1 0 7 234 7 773.1 0 7 235 16 773.1 0 16 236 11 773.1 0 11 237 83 773.1 0 83 238 16 773.1 0 16 239 19 773.1 0 19 240 18 773.1 0 18 241 20 773.1 0 20 242 24 773.1 0 24 243 24 773.1 0 24 244 14 773.1 0 14 245 4 773.1 0 4 246 5 773.1 0 5 247 8 773.1 0 8 248 13 773.1 0 13 249 15 773.1 0 15 250 11 773.1 0 11 251 17 773.1 0 17 252 26 773.1 0 26 253 11 773.1 0 11 254 17 773.1 0 17 255 28 773.1 0 28 256 66 773.1 0 66 257 61 773.1 0 61 258 88 773.1 0 88 259 31 773.1 0 31 260 28 773.1 0 28 261 14 773.1 0 14 262 31 773.1 0 31 263 17 773.1 0 17 264 25 773.1 0 25 265 8 773.1 0 8 266 17 773.1 0 17 267 13 773.1 0 13 268 12 773.1 0 12 269 20 773.1 0 20 270 21 773.1 0 21 271 16 773.1 0 16 272 12 773.1 0 12 273 9 773.1 0 9 274 15 773.1 0 15 275 16 773.1 0 16 276 21 773.1 0 21 277 24 773.1 0 24 278 25 773.1 0 25 279 14 773.1 0 14 280 17 773.1 0 17 281 9 773.1 0 9 282 14 773.1 0 14 283 15 773.1 0 15 284 15 773.1 0 15 285 18 773.1 0 18 286 20 773.1 0 20 287 21 773.1 0 21 288 20 773.1 0 20 289 29 773.1 0 29 290 44 773.1 0 44 291 42 773.1 0 42 292 31 773.1 0 31 293 22 773.1 0 22 294 19 773.1 0 19 295 27 773.1 0 27 296 31 773.1 0 31 297 30 773.1 0 30 298 28 773.1 0 28 299 35 773.1 0 35 300 57 773.1 0 57 301 25 773.1 0 25 Finished in 11.82 s (78 us/read; 0.77 M reads/minute). === Summary === Total reads processed: 151,062 Reads with adapters: 86,687 (57.4%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 86,675 (57.4%) Total basepairs processed: 43,526,838 bp Total written (filtered): 23,544,004 bp (54.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 86687 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 8 0.0 3 0 0 6 2 19 200 0.0 3 0 196 1 0 3 20 81570 0.0 4 80317 1191 55 3 4 21 4449 0.0 4 4238 200 9 1 1 22 25 0.0 4 0 10 15 23 47 0.0 4 1 0 0 41 5 24 5 0.0 4 1 0 0 2 2 25 2 0.0 4 2 32 1 0.0 4 1 36 1 0.0 4 1 41 1 0.0 4 1 43 1 0.0 4 1 107 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 127 62 0.0 4 0 0 0 0 62 128 299 0.0 4 0 0 0 0 299 129 2 0.0 4 0 0 0 0 2 152 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 273 1 0.0 4 1 282 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.96 s (57 us/read; 1.05 M reads/minute). === Summary === Total reads processed: 86,675 Reads with adapters: 13,943 (16.1%) Reads written (passing filters): 86,675 (100.0%) Total basepairs processed: 23,544,004 bp Total written (filtered): 23,408,360 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 13943 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.5% C: 2.0% G: 96.5% T: 1.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 10 1354.3 0 10 4 12 338.6 0 12 5 8 84.6 0 8 6 15 21.2 0 15 7 1 5.3 0 1 8 4 1.3 0 4 9 9142 0.3 0 162 8980 10 3897 0.1 1 109 3788 11 819 0.0 1 6 813 12 7 0.0 1 1 6 13 1 0.0 1 0 1 14 1 0.0 1 0 1 32 1 0.0 1 1 69 1 0.0 1 1 81 1 0.0 1 1 150 5 0.0 1 0 5 208 1 0.0 1 0 1 210 1 0.0 1 0 1 220 1 0.0 1 0 1 223 11 0.0 1 11 224 1 0.0 1 1 258 1 0.0 1 1 259 1 0.0 1 1 265 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.