This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATTCTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 147.63 s (47 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 337,106 (10.6%) Reads that were too short: 5,133 (0.2%) Reads written (passing filters): 331,973 (10.5%) Total basepairs processed: 953,096,634 bp Total written (filtered): 57,543,588 bp (6.0%) === Adapter 1 === Sequence: ATTCTA; Type: regular 5'; Length: 6; Trimmed: 337106 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 379 773.1 0 379 7 20064 773.1 0 20064 8 17809 773.1 0 17809 9 24 773.1 0 24 10 16 773.1 0 16 11 161 773.1 0 161 12 127 773.1 0 127 13 280 773.1 0 280 14 15 773.1 0 15 15 14 773.1 0 14 16 7 773.1 0 7 17 8 773.1 0 8 18 15 773.1 0 15 19 18 773.1 0 18 20 26 773.1 0 26 21 16 773.1 0 16 22 19 773.1 0 19 23 6 773.1 0 6 24 7 773.1 0 7 25 37 773.1 0 37 26 36 773.1 0 36 27 43 773.1 0 43 28 9 773.1 0 9 29 4 773.1 0 4 30 7 773.1 0 7 31 32 773.1 0 32 32 20 773.1 0 20 33 23 773.1 0 23 34 27 773.1 0 27 35 19 773.1 0 19 36 15 773.1 0 15 37 20 773.1 0 20 38 48 773.1 0 48 39 19 773.1 0 19 40 51 773.1 0 51 41 7 773.1 0 7 42 11 773.1 0 11 43 43 773.1 0 43 44 21 773.1 0 21 45 16 773.1 0 16 46 26 773.1 0 26 47 8 773.1 0 8 48 18 773.1 0 18 49 7 773.1 0 7 50 8 773.1 0 8 51 5 773.1 0 5 52 12 773.1 0 12 53 17 773.1 0 17 54 19 773.1 0 19 55 20 773.1 0 20 56 10 773.1 0 10 57 16 773.1 0 16 58 20 773.1 0 20 59 19 773.1 0 19 60 15 773.1 0 15 61 16 773.1 0 16 62 11 773.1 0 11 63 9 773.1 0 9 64 9 773.1 0 9 65 8 773.1 0 8 66 5 773.1 0 5 67 7 773.1 0 7 68 7 773.1 0 7 69 8 773.1 0 8 70 21 773.1 0 21 71 24 773.1 0 24 72 35 773.1 0 35 73 52 773.1 0 52 74 35 773.1 0 35 75 76 773.1 0 76 76 28 773.1 0 28 77 14 773.1 0 14 78 12 773.1 0 12 79 13 773.1 0 13 80 18 773.1 0 18 81 11 773.1 0 11 82 16 773.1 0 16 83 10 773.1 0 10 84 16 773.1 0 16 85 21 773.1 0 21 86 20 773.1 0 20 87 6 773.1 0 6 88 12 773.1 0 12 89 34 773.1 0 34 90 15 773.1 0 15 91 27 773.1 0 27 92 15 773.1 0 15 93 15 773.1 0 15 94 18 773.1 0 18 95 20 773.1 0 20 96 21 773.1 0 21 97 53 773.1 0 53 98 56 773.1 0 56 99 39 773.1 0 39 100 31 773.1 0 31 101 19 773.1 0 19 102 85 773.1 0 85 103 62 773.1 0 62 104 86 773.1 0 86 105 103 773.1 0 103 106 79 773.1 0 79 107 88 773.1 0 88 108 64 773.1 0 64 109 47 773.1 0 47 110 35 773.1 0 35 111 55 773.1 0 55 112 72 773.1 0 72 113 45 773.1 0 45 114 87 773.1 0 87 115 259 773.1 0 259 116 30 773.1 0 30 117 23 773.1 0 23 118 29 773.1 0 29 119 28 773.1 0 28 120 46 773.1 0 46 121 96 773.1 0 96 122 476 773.1 0 476 123 360 773.1 0 360 124 426 773.1 0 426 125 379 773.1 0 379 126 95 773.1 0 95 127 221 773.1 0 221 128 150 773.1 0 150 129 196 773.1 0 196 130 605 773.1 0 605 131 176 773.1 0 176 132 136 773.1 0 136 133 74 773.1 0 74 134 112 773.1 0 112 135 299 773.1 0 299 136 331 773.1 0 331 137 594 773.1 0 594 138 882 773.1 0 882 139 1210 773.1 0 1210 140 2758 773.1 0 2758 141 6652 773.1 0 6652 142 106795 773.1 0 106795 143 61634 773.1 0 61634 144 81361 773.1 0 81361 145 387 773.1 0 387 146 91 773.1 0 91 147 72 773.1 0 72 148 37 773.1 0 37 149 58 773.1 0 58 150 68 773.1 0 68 151 47 773.1 0 47 152 25 773.1 0 25 153 24 773.1 0 24 154 23 773.1 0 23 155 41 773.1 0 41 156 61 773.1 0 61 157 97 773.1 0 97 158 88 773.1 0 88 159 151 773.1 0 151 160 281 773.1 0 281 161 627 773.1 0 627 162 8986 773.1 0 8986 163 5391 773.1 0 5391 164 6305 773.1 0 6305 165 36 773.1 0 36 166 21 773.1 0 21 167 42 773.1 0 42 168 26 773.1 0 26 169 41 773.1 0 41 170 24 773.1 0 24 171 13 773.1 0 13 172 14 773.1 0 14 173 4 773.1 0 4 174 4 773.1 0 4 175 6 773.1 0 6 176 22 773.1 0 22 177 58 773.1 0 58 178 23 773.1 0 23 179 18 773.1 0 18 180 21 773.1 0 21 181 9 773.1 0 9 182 22 773.1 0 22 183 31 773.1 0 31 184 22 773.1 0 22 185 17 773.1 0 17 186 20 773.1 0 20 187 35 773.1 0 35 188 171 773.1 0 171 189 88 773.1 0 88 190 178 773.1 0 178 191 19 773.1 0 19 192 9 773.1 0 9 193 9 773.1 0 9 194 19 773.1 0 19 195 16 773.1 0 16 196 25 773.1 0 25 197 15 773.1 0 15 198 18 773.1 0 18 199 21 773.1 0 21 200 16 773.1 0 16 201 17 773.1 0 17 202 31 773.1 0 31 203 22 773.1 0 22 204 20 773.1 0 20 205 19 773.1 0 19 206 20 773.1 0 20 207 21 773.1 0 21 208 30 773.1 0 30 209 49 773.1 0 49 210 54 773.1 0 54 211 51 773.1 0 51 212 53 773.1 0 53 213 64 773.1 0 64 214 187 773.1 0 187 215 307 773.1 0 307 216 251 773.1 0 251 217 97 773.1 0 97 218 31 773.1 0 31 219 18 773.1 0 18 220 16 773.1 0 16 221 12 773.1 0 12 222 31 773.1 0 31 223 55 773.1 0 55 224 42 773.1 0 42 225 45 773.1 0 45 226 38 773.1 0 38 227 50 773.1 0 50 228 21 773.1 0 21 229 15 773.1 0 15 230 17 773.1 0 17 231 13 773.1 0 13 232 45 773.1 0 45 233 58 773.1 0 58 234 58 773.1 0 58 235 22 773.1 0 22 236 21 773.1 0 21 237 25 773.1 0 25 238 39 773.1 0 39 239 49 773.1 0 49 240 48 773.1 0 48 241 26 773.1 0 26 242 21 773.1 0 21 243 39 773.1 0 39 244 29 773.1 0 29 245 38 773.1 0 38 246 39 773.1 0 39 247 23 773.1 0 23 248 20 773.1 0 20 249 23 773.1 0 23 250 20 773.1 0 20 251 28 773.1 0 28 252 18 773.1 0 18 253 15 773.1 0 15 254 23 773.1 0 23 255 63 773.1 0 63 256 69 773.1 0 69 257 56 773.1 0 56 258 57 773.1 0 57 259 37 773.1 0 37 260 62 773.1 0 62 261 182 773.1 0 182 262 163 773.1 0 163 263 218 773.1 0 218 264 66 773.1 0 66 265 30 773.1 0 30 266 22 773.1 0 22 267 19 773.1 0 19 268 26 773.1 0 26 269 57 773.1 0 57 270 54 773.1 0 54 271 40 773.1 0 40 272 20 773.1 0 20 273 30 773.1 0 30 274 36 773.1 0 36 275 49 773.1 0 49 276 73 773.1 0 73 277 50 773.1 0 50 278 47 773.1 0 47 279 116 773.1 0 116 280 117 773.1 0 117 281 153 773.1 0 153 282 64 773.1 0 64 283 56 773.1 0 56 284 54 773.1 0 54 285 62 773.1 0 62 286 30 773.1 0 30 287 40 773.1 0 40 288 18 773.1 0 18 289 27 773.1 0 27 290 49 773.1 0 49 291 23 773.1 0 23 292 39 773.1 0 39 293 35 773.1 0 35 294 31 773.1 0 31 295 42 773.1 0 42 296 67 773.1 0 67 297 60 773.1 0 60 298 45 773.1 0 45 299 24 773.1 0 24 300 33 773.1 0 33 301 15 773.1 0 15 Finished in 17.75 s (53 us/read; 1.12 M reads/minute). === Summary === Total reads processed: 331,973 Reads with adapters: 20,047 (6.0%) Reads that were too short: 72 (0.0%) Reads written (passing filters): 19,975 (6.0%) Total basepairs processed: 57,543,588 bp Total written (filtered): 5,525,772 bp (9.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 20047 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 23 0.0 3 0 0 21 2 16 391 0.0 3 0 349 35 7 17 19392 0.0 3 18402 829 129 32 18 59 0.0 3 4 36 11 8 19 47 0.0 3 4 0 18 25 20 11 0.0 3 1 0 0 10 21 1 0.0 3 0 0 1 23 1 0.0 3 0 0 1 25 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 30 2 0.0 3 0 0 0 2 32 1 0.0 3 1 34 1 0.0 3 0 0 1 35 1 0.0 3 1 36 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 59 17 0.0 3 0 0 0 17 60 2 0.0 3 0 0 0 2 61 2 0.0 3 0 0 0 2 72 2 0.0 3 0 0 0 2 76 1 0.0 3 0 0 1 78 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 82 2 0.0 3 1 0 0 1 83 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 1 1 90 2 0.0 3 0 0 0 2 92 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 144 5 0.0 3 0 0 0 5 145 1 0.0 3 0 0 0 1 146 5 0.0 3 0 0 0 5 147 11 0.0 3 0 0 0 11 149 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 155 2 0.0 3 0 0 1 1 156 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 2 0.0 3 0 0 0 2 163 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 180 3 0.0 3 0 0 0 3 181 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 2 0.0 3 0 0 0 2 205 2 0.0 3 0 0 0 2 207 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 4 0.0 3 0 0 0 4 219 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 274 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 1 1 282 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.09 s (55 us/read; 1.10 M reads/minute). === Summary === Total reads processed: 19,975 Reads with adapters: 231 (1.2%) Reads written (passing filters): 19,975 (100.0%) Total basepairs processed: 5,525,772 bp Total written (filtered): 5,525,074 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 231 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 12.1% C: 77.5% G: 7.8% T: 2.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 228 312.1 0 228 4 2 78.0 0 2 6 1 4.9 0 1