This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATTCTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 148.02 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 371,323 (11.7%) Reads that were too short: 3,604 (0.1%) Reads written (passing filters): 367,719 (11.6%) Total basepairs processed: 953,096,634 bp Total written (filtered): 63,290,720 bp (6.6%) === Adapter 1 === Sequence: ATTCTA; Type: regular 5'; Length: 6; Trimmed: 371323 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 337 773.1 0 337 7 17114 773.1 0 17114 8 22042 773.1 0 22042 9 15 773.1 0 15 10 6 773.1 0 6 11 43 773.1 0 43 12 89 773.1 0 89 13 101 773.1 0 101 14 11 773.1 0 11 15 8 773.1 0 8 16 12 773.1 0 12 17 13 773.1 0 13 18 13 773.1 0 13 19 10 773.1 0 10 20 25 773.1 0 25 21 16 773.1 0 16 22 19 773.1 0 19 23 9 773.1 0 9 24 14 773.1 0 14 25 12 773.1 0 12 26 13 773.1 0 13 27 31 773.1 0 31 28 14 773.1 0 14 29 6 773.1 0 6 30 6 773.1 0 6 31 13 773.1 0 13 32 8 773.1 0 8 33 8 773.1 0 8 34 29 773.1 0 29 35 11 773.1 0 11 36 5 773.1 0 5 37 5 773.1 0 5 38 5 773.1 0 5 39 5 773.1 0 5 40 12 773.1 0 12 41 9 773.1 0 9 42 10 773.1 0 10 43 36 773.1 0 36 44 23 773.1 0 23 45 21 773.1 0 21 46 13 773.1 0 13 47 8 773.1 0 8 48 9 773.1 0 9 49 7 773.1 0 7 50 6 773.1 0 6 51 12 773.1 0 12 52 7 773.1 0 7 53 10 773.1 0 10 54 25 773.1 0 25 55 20 773.1 0 20 56 8 773.1 0 8 57 11 773.1 0 11 58 17 773.1 0 17 59 19 773.1 0 19 60 16 773.1 0 16 61 24 773.1 0 24 62 8 773.1 0 8 63 11 773.1 0 11 64 17 773.1 0 17 65 8 773.1 0 8 66 11 773.1 0 11 67 8 773.1 0 8 68 13 773.1 0 13 69 5 773.1 0 5 70 15 773.1 0 15 71 19 773.1 0 19 72 35 773.1 0 35 73 36 773.1 0 36 74 34 773.1 0 34 75 65 773.1 0 65 76 26 773.1 0 26 77 8 773.1 0 8 78 13 773.1 0 13 79 11 773.1 0 11 80 8 773.1 0 8 81 8 773.1 0 8 82 14 773.1 0 14 83 5 773.1 0 5 84 14 773.1 0 14 85 24 773.1 0 24 86 21 773.1 0 21 87 18 773.1 0 18 88 19 773.1 0 19 89 47 773.1 0 47 90 24 773.1 0 24 91 48 773.1 0 48 92 22 773.1 0 22 93 23 773.1 0 23 94 25 773.1 0 25 95 19 773.1 0 19 96 18 773.1 0 18 97 35 773.1 0 35 98 53 773.1 0 53 99 35 773.1 0 35 100 30 773.1 0 30 101 15 773.1 0 15 102 64 773.1 0 64 103 29 773.1 0 29 104 61 773.1 0 61 105 117 773.1 0 117 106 62 773.1 0 62 107 64 773.1 0 64 108 52 773.1 0 52 109 35 773.1 0 35 110 31 773.1 0 31 111 39 773.1 0 39 112 77 773.1 0 77 113 44 773.1 0 44 114 45 773.1 0 45 115 262 773.1 0 262 116 35 773.1 0 35 117 16 773.1 0 16 118 27 773.1 0 27 119 22 773.1 0 22 120 31 773.1 0 31 121 54 773.1 0 54 122 527 773.1 0 527 123 267 773.1 0 267 124 372 773.1 0 372 125 454 773.1 0 454 126 119 773.1 0 119 127 206 773.1 0 206 128 159 773.1 0 159 129 190 773.1 0 190 130 664 773.1 0 664 131 225 773.1 0 225 132 106 773.1 0 106 133 72 773.1 0 72 134 118 773.1 0 118 135 284 773.1 0 284 136 353 773.1 0 353 137 584 773.1 0 584 138 837 773.1 0 837 139 1253 773.1 0 1253 140 2769 773.1 0 2769 141 5993 773.1 0 5993 142 122599 773.1 0 122599 143 70833 773.1 0 70833 144 92968 773.1 0 92968 145 383 773.1 0 383 146 48 773.1 0 48 147 48 773.1 0 48 148 31 773.1 0 31 149 46 773.1 0 46 150 53 773.1 0 53 151 29 773.1 0 29 152 20 773.1 0 20 153 16 773.1 0 16 154 19 773.1 0 19 155 34 773.1 0 34 156 56 773.1 0 56 157 70 773.1 0 70 158 75 773.1 0 75 159 129 773.1 0 129 160 260 773.1 0 260 161 479 773.1 0 479 162 8772 773.1 0 8772 163 5366 773.1 0 5366 164 6417 773.1 0 6417 165 38 773.1 0 38 166 19 773.1 0 19 167 31 773.1 0 31 168 14 773.1 0 14 169 27 773.1 0 27 170 16 773.1 0 16 171 7 773.1 0 7 172 6 773.1 0 6 173 2 773.1 0 2 174 9 773.1 0 9 175 12 773.1 0 12 176 24 773.1 0 24 177 39 773.1 0 39 178 25 773.1 0 25 179 10 773.1 0 10 180 9 773.1 0 9 181 12 773.1 0 12 182 7 773.1 0 7 183 16 773.1 0 16 184 18 773.1 0 18 185 19 773.1 0 19 186 11 773.1 0 11 187 16 773.1 0 16 188 182 773.1 0 182 189 85 773.1 0 85 190 191 773.1 0 191 191 6 773.1 0 6 192 11 773.1 0 11 193 6 773.1 0 6 194 10 773.1 0 10 195 19 773.1 0 19 196 20 773.1 0 20 197 20 773.1 0 20 198 11 773.1 0 11 199 14 773.1 0 14 200 16 773.1 0 16 201 12 773.1 0 12 202 18 773.1 0 18 203 20 773.1 0 20 204 19 773.1 0 19 205 18 773.1 0 18 206 21 773.1 0 21 207 17 773.1 0 17 208 31 773.1 0 31 209 21 773.1 0 21 210 39 773.1 0 39 211 32 773.1 0 32 212 31 773.1 0 31 213 27 773.1 0 27 214 123 773.1 0 123 215 156 773.1 0 156 216 132 773.1 0 132 217 51 773.1 0 51 218 12 773.1 0 12 219 17 773.1 0 17 220 11 773.1 0 11 221 15 773.1 0 15 222 16 773.1 0 16 223 35 773.1 0 35 224 38 773.1 0 38 225 31 773.1 0 31 226 31 773.1 0 31 227 49 773.1 0 49 228 21 773.1 0 21 229 19 773.1 0 19 230 8 773.1 0 8 231 11 773.1 0 11 232 27 773.1 0 27 233 36 773.1 0 36 234 38 773.1 0 38 235 20 773.1 0 20 236 22 773.1 0 22 237 21 773.1 0 21 238 22 773.1 0 22 239 26 773.1 0 26 240 27 773.1 0 27 241 24 773.1 0 24 242 18 773.1 0 18 243 26 773.1 0 26 244 25 773.1 0 25 245 29 773.1 0 29 246 19 773.1 0 19 247 20 773.1 0 20 248 15 773.1 0 15 249 15 773.1 0 15 250 18 773.1 0 18 251 19 773.1 0 19 252 17 773.1 0 17 253 20 773.1 0 20 254 21 773.1 0 21 255 42 773.1 0 42 256 37 773.1 0 37 257 48 773.1 0 48 258 31 773.1 0 31 259 34 773.1 0 34 260 36 773.1 0 36 261 145 773.1 0 145 262 140 773.1 0 140 263 198 773.1 0 198 264 37 773.1 0 37 265 14 773.1 0 14 266 12 773.1 0 12 267 22 773.1 0 22 268 17 773.1 0 17 269 35 773.1 0 35 270 20 773.1 0 20 271 32 773.1 0 32 272 7 773.1 0 7 273 26 773.1 0 26 274 17 773.1 0 17 275 24 773.1 0 24 276 33 773.1 0 33 277 33 773.1 0 33 278 30 773.1 0 30 279 93 773.1 0 93 280 71 773.1 0 71 281 143 773.1 0 143 282 38 773.1 0 38 283 45 773.1 0 45 284 33 773.1 0 33 285 38 773.1 0 38 286 18 773.1 0 18 287 31 773.1 0 31 288 24 773.1 0 24 289 20 773.1 0 20 290 37 773.1 0 37 291 18 773.1 0 18 292 33 773.1 0 33 293 25 773.1 0 25 294 33 773.1 0 33 295 39 773.1 0 39 296 56 773.1 0 56 297 57 773.1 0 57 298 34 773.1 0 34 299 17 773.1 0 17 300 22 773.1 0 22 301 14 773.1 0 14 Finished in 20.76 s (56 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 367,719 Reads with adapters: 22,761 (6.2%) Reads that were too short: 11 (0.0%) Reads written (passing filters): 22,750 (6.2%) Total basepairs processed: 63,290,720 bp Total written (filtered): 6,182,070 bp (9.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 22761 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 3 0.0 3 0 0 0 0 3 19 54 0.0 3 0 51 3 20 22356 0.0 4 22043 303 9 0 1 21 47 0.0 4 7 38 2 22 7 0.0 4 4 1 2 23 13 0.0 4 0 0 0 12 1 25 2 0.0 4 2 26 1 0.0 4 1 30 1 0.0 4 1 37 6 0.0 4 0 0 0 0 6 38 5 0.0 4 0 0 0 0 5 46 1 0.0 4 0 0 0 1 47 1 0.0 4 0 0 0 1 70 1 0.0 4 0 0 0 0 1 81 2 0.0 4 0 0 0 0 2 86 1 0.0 4 1 108 1 0.0 4 0 0 0 0 1 123 3 0.0 4 0 0 0 0 3 125 1 0.0 4 0 0 0 0 1 127 248 0.0 4 0 0 0 0 248 130 1 0.0 4 0 0 0 0 1 181 1 0.0 4 1 228 2 0.0 4 0 0 0 0 2 234 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.30 s (57 us/read; 1.05 M reads/minute). === Summary === Total reads processed: 22,750 Reads with adapters: 1,876 (8.2%) Reads written (passing filters): 22,750 (100.0%) Total basepairs processed: 6,182,070 bp Total written (filtered): 6,160,973 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1876 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.0% C: 3.3% G: 94.0% T: 1.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 5 355.5 0 5 4 7 88.9 0 7 6 1 5.6 0 1 9 8 0.1 0 0 8 10 281 0.0 1 8 273 11 1565 0.0 1 7 1558 12 3 0.0 1 0 3 14 1 0.0 1 0 1 151 3 0.0 1 0 3 224 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.